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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for lefty A TGF beta 4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Hs06560655 (human) (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg/ml for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FBS (0.01 M HEPES, 1x sodium pyruvate, 0.05 mM 2-beta-mercaptoethanol (Gibco) and 0.1 mg/ml Penicillin-streptomycin (Sigma ...
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bioRxiv - Microbiology 2021Quote: The IFN-β levels in BALF were determined using mouse IFN beta ProQuantum Immunoassay kit (ThermoFisher). IFN-α and IFN-β levels in the supernatants of cultured BMDCs were determined by ELISA (PBL Biomedical Laboratories).
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bioRxiv - Developmental Biology 2022Quote: ... ELISA was performed using the ultrasensitive Amyloid beta ELISA kit from Invitrogen (Paina et al.,2011KHB3544) per manufacturer’s instructions with media samples diluted 1:2 in standard diluent buffer ...
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bioRxiv - Bioengineering 2021Quote: ... Neuronal bodies and projections were labeled with antibodies against Beta-3 Tubulin (mouse monoclonal, Thermo Fisher, MA1-118 ...
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bioRxiv - Neuroscience 2024Quote: ... Levels of secreted amyloid-beta 1-42 were quantified using a sandwich ELISA kit (Invitrogen, USA) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... ULBP6 (Hs04194671_s1) and beta actin (Hs99999903_m1) with TaqMan MGB probes all conjugated with fluorochrome FAM (Thermofisher). The cycling conditions were 50°C for 2 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Beta-Mercaptoethanol Thermo Fisher Scientific 21985023) with a SMAD inhibitor (SB-431542 Fisher Scientific 16-141) and WNT activator (CHIR-99021 Fisher Scientific S12632) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated with 3X cholesterol or 5mM methyl beta cyclodextrin (MßC) (#377110050, Thermo Fisher) (doses chosen for biological effectiveness but minimal cellular toxicity [Supplementary Figures 2 and 3] ...
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bioRxiv - Bioengineering 2024Quote: ... and TGF-β1 conditions using a lysis buffer solution containing Pierce RIPA Buffer and Halt Protease & Phosphatase Inhibitor Cocktail (Thermo Scientific; Waltham, MA). Sample lysates were mixed for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... cells were pulsed in starvation buffer supplemented with 0.1% w/v bovine serum albumin at 4 °C for 40 min with 50 ng/mL human recombinant EGF (Thermo-Fisher, Gibco™, PHG0311L) to allow ligand bind to the EGFR ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated 20 min at 4°C with AF647-conjugated goat anti-human IgG antibodies (1:1000 dilution; Thermo Fisher Scientific) and LIVE/DEAD Fixable Viability dye Aqua (1:1000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... AlexaFluor488-labeled human transferrin was dissolved in PBS 5 at mg/ml and stored at 4°C (Thermo Fisher, ref. no. 13342). TexasRed-labelled neutral 3’000 and 40’000 Da dextran was dissolved in PBS at 10 mg/ml and stored at -20°C (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... we use 1µg/ml final concentration of ethidium bromide to stain parasitized erythrocytes and 4 µl/ml Alexa Fluor 488-conjugated goat anti-human IgG (H+L) (Invitrogen, Life Technologies), based on optimal signal to noise between positive control plasma (10 Mali highly exposed individual plasma ...
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bioRxiv - Cell Biology 2023Quote: ... E6 medium was changed daily up to 4 days before switching to human endothelial serum free medium (hESFM) (Thermo Fisher Scientific, 11111) supplemented with 10nM bFGF (Fibroblast growth factor ...
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bioRxiv - Immunology 2024Quote: ... we co-transfected 3×106 3-day activated CD8+T cells with 150 pmol/106 cells of human TSP-4-specific siRNAs (#s14100, Thermo Fisher Scientific) and 1.5 μg/106 of the pMax-TSP-1-GFPSpark construct ...
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bioRxiv - Immunology 2024Quote: ... Program T-023 with 150 pmol/106 cells of human TSP-1 and TSP-4-specific siRNAs (#s14108 and #s14100, Thermo Fisher Scientific) and control scrambled siRNAs (#4390846 ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µg of total RNA from HEK293 cells or tissue samples was treated with DNAseI Free kit (Thermofisher) followed by cDNA generation using the High capacity cDNA kit (Thermofisher) ...
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bioRxiv - Biochemistry 2020Quote: Dvl2-GFP/dsRed-Axin1 HEK293 cells were seeded at ∼60% confluence in Lab-Tek chamber slides (Nunc 177380) that were pre-coated with 0.1 mg/mL poly-D-lysine (Sigma P6407) ...
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bioRxiv - Biophysics 2019Quote: ... The protein was expressed in HEK293S GnTI− cells (ATCC CRL-3022) grown in Expi293 Expression Media (Life Technologies) to densities 2.5 × 106 cells ml−1 ...
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bioRxiv - Biochemistry 2020Quote: The CR3 headpiece stably transfected HEK293S cells were kept as adhesion cell culture growing in Dulbecco’s Modification of Eagle’s Medium (DMEM) GlutaMAX (Gibco) supplemented with 10 % (v/v ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were grown in DMEM with 10% fetal bovine serum (FBS) and 1:100 penicillin-streptomycin (ThermoFisher). In 24-well plates ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Mutations introduced into the δ-subunit were established as stable HEK293 Flp-InTM cell lines (Invitrogen, Paisley, UK), generating a stable cell line for each mutant ...
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bioRxiv - Neuroscience 2020Quote: HEK293 and SH-SY5Y cells (ATCC) were cultured in phenol red-free Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 2 mM L-Glutamine (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: HEK293 or HeLa cells were co-transfected with pBABED and pX335 plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Human Embryonic Kidney 293 (HEK293) cells (ATCC CRL-1573) were cultured in complete growth Dulbecco’s modified Eagle’s medium (DMEM, Gibco), supplemented with 10% fetal bovine serum (UCSF Cell Culture Facility) ...
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bioRxiv - Microbiology 2021Quote: HeLa cells (ATCC CCL-2) and HEK293 cells (ATCC CRL-1573) were maintained in DMEM high glucose (Gibco) containing 10% heat-inactivated fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant constructs were transfected into HEK293 cells either individually or together using Lipofectamine® 3000 reagent (Invitrogen) following the instructions provided ...
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bioRxiv - Systems Biology 2022Quote: ... HEK293 cells were prepared as 2 × 105 cells/mL in DMEM (Dulbecco s Modified Eagle Media, Life Technologies) + 10% FBS (Foetal Bovine Serum ...
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bioRxiv - Biophysics 2022Quote: ... The Fab version of the antibody fusion (Fab-ShK) was expressed in freestyle HEK293 (293F) cells (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Human embryonic kidney cells (HEK293) were cultured in culture flask (150 cm2) to 80 % confluence in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) supplemented with 50 units/ml penicillin G (Gibco) ...
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bioRxiv - Biochemistry 2020Quote: ... HELA cells (DSMZ ACC-57) and HEK293 cells (DSMZ ACC-305) were cultured in DMEM (Thermo Fisher Scientific) supplemented with 10% FCS ...
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bioRxiv - Molecular Biology 2020Quote: ... and renilla luciferase plasmid (1 ng) were transfected into HEK293 cells or HCT116 cells by lipofectamine2000 (Invitrogen, 11668030) following manufacturer’s manual ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293-T cells were seeded into 10 cm plates with DMEM containing 0.5 mg/mL geneticin G418 (Thermofisher). Once the cells reached approximately 80% confluence ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 FcγRII cells (a gift from Dr. Craig Roy) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% heat-inactivated fetal bovine serum (HIFBS ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 Flp-In T-Rex cells were co-transfected with pOG44 Flp-Recombinase Expression Vector (Invitrogen™, V600520), and pcDNA5 FRT TO plasmids expressing appropriate miniTurbo gene fusions ...
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bioRxiv - Neuroscience 2019Quote: HEK293 and SH-SY5Y cells (ATCC) were cultured in phenol red-free Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 2 mM L-Glutamine (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected with EBV-BAC DNA using Lipofectamine 2000 reagent (Thermo Fisher Scientific, Waltham, MA, USA) followed by hygromycin selection ...
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bioRxiv - Cell Biology 2020Quote: ... siRNA knockdown of AC1 and AC9 expression in HEK293 cells was carried out using Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: Two HEK293 cell lines stably expressing mouse STAC3 were generated using the Flp-In T-Rex system (Invitrogen). Host cells ...
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal 8xHis-TwinStrep-GFP-tagged NKCC1 constructs were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in 24 well plates ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 Flp-In™ T-REx™ cell lines were generated according to the manufacturer’s instructions (ThermoFisher Scientific). BirA-containing plasmids (0.4 µg) ...
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bioRxiv - Genomics 2022Quote: Adherent HEK293 cell lines were grown in 10 mLs of DMEM (ATCC 302002) containing 10% FBS (Gibco 16000044) and 1% Pen/Strep (Gibco 15070063) ...
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bioRxiv - Microbiology 2022Quote: ... as well as the HEK293 cell line were grown in Dulbecco’s Modified Eagle’s Medium F-12 50/50 (DMEM/F-12, GIBCO) supplemented with 10% FBS at 37°C with 5% CO2 in a humidified chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... Lentivirus production was performed according to the manufacturer’s recommendations in HEK293 E cells using Lipofectamine 3000 (ThermoFisher Scientific). Virus-containing supernatant was collected after 24 and 48 h and combined ...
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bioRxiv - Immunology 2023Quote: HEK293 cells (ATCC CRL-1573) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Paneco) supplemented with glutamine (Gibco), 10% fetal bovine serum (HyClone) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Stable HEK293 cell lines were achieved by antibiotic selection with G-418 (1mg/ml, Gibco-BRL, Paisley, UK) added to the medium 3 days after transfection ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK293 cells were cultured in a 2L suspension using FreeStyle 293 Expression Medium (Life Technologies, Carlsbad, CA, USA) with a cell density maintained at 1 × 10^6 cells/mL on the day of transfection ...
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bioRxiv - Microbiology 2024Quote: ... HEK293 ACE2 and lung carcinoma epithelial cells A549 ACE2 were cultivated with Dulbecco’s Modified Eagle Medium (DMEM; Gibco) with 10% fetal bovine serum (Sigma-Alrich ...