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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for lefty A TGF beta 4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s serum (DMEM) (Thermo Fisher Scientific, Oberhausen, Germany) supplemented with 10% FBS (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... pH change experiment for oROS-HT-C199S were performed with HEK293s in PBS (10010001, Gibco) prepared at pH of 6 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HEK293 cells were transiently transfected following the reverse transfection method using LipofectamineTM 3000 (Thermo Fisher). Two transfection mixes (A & B ...
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bioRxiv - Biochemistry 2024Quote: SH-SY5Y and HEK293/T cells were transfected with siRNA using Lipofectamine 3000 (Thermo Fisher) per the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Transfections were performed in HEK293 cells using Lipofectamine™ 3000 Transfection Reagent (L3000015, Thermo Fisher) and serum-free Opti-MEM I medium (31985 ...
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bioRxiv - Neuroscience 2024Quote: ... sgRNAs were tested for efficacy in HEK293 (ATCC) using a genomic cleavage kit (GeneArt, ThermoFisher). The best sgRNA was used for editing and had the sequence ...
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bioRxiv - Microbiology 2024Quote: ... and human (goat anti-human IgG, ThermoFisher) were also used ...
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bioRxiv - Neuroscience 2020Quote: Human sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and coverslips were mounted using Prolong Gold (Invitrogen) for imaging ...
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bioRxiv - Microbiology 2021Quote: ... falciparum NF54 parasites were cultivated at 4% hematocrit in human B+ blood in RPMI-HEPES supplemented with 0.25% Albumax 1 (Invitrogen) and human donor plasma type B+ ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended in S12 media supplemented with 4 mg/L Recombinant Human Insulin (ThermoFisher Scientific, Waltham, MA, USA) (S12+ media ...
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bioRxiv - Immunology 2023Quote: ... purified cells were plated in 96-well round bottom plate in Yssel’s media (IMDM + 4% v/v Yssel’s supplement (made in house, Amsterdam UMC) + 1% v/v human AB serum (Invitrogen)) ...
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bioRxiv - Immunology 2023Quote: CHO cells that were stably transfected with human CTLA-4 have been reported.23 CHO cells were grown in DMEM (Dulbecco’s Modified Eagle Medium, Gibco) supplemented with 10% FBS (Hyclone) ...
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bioRxiv - Immunology 2023Quote: ... human stool extracts and mouse colonic tissue lysates were resolved on Bolt 4-12% Bis-Tris Plus Gels (Invitrogen), transferred onto polyvinylidene difluoride membranes ...
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bioRxiv - Immunology 2023Quote: ... and TNF-α (cat#BMS223-4) were measured in duplicate using human ELISA kits following the manufacturer’s instructions (ThermoFisher) and SpectraMax M3 (Molecular devices).
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bioRxiv - Microbiology 2024Quote: T24 human epithelial bladder cells (ATCC HTB-4) were grown in McCoy’s 5A modified medium (Gibco 16-600-082) with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2019Quote: ... from the 24-well plate were trypsinized and plated as passage 1 cells at a density of ∼3,000 cells/cm2 in media supplemented with 1 ng/ml TGF-β1 (PHG9214, Life technologies) and 5 ng/ml bFGF (PHG0264 ...
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bioRxiv - Microbiology 2023Quote: ... lyophilized and resuspended in Procartaplex Universal Assay Buffer and stored at −80°C prior to measurement of TGF-B using Procartaplex Single-plex assay (ThermoFisher).
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bioRxiv - Immunology 2019Quote: ... Samples were prepared using the Ion PGM™ Hi-Q™ View OT2 and Ion PGM™ Hi-Q™ View Sequencing kits (ThermoFisher Scientific) and 4 barcoded libraries were combined per Ion 316™ Chip kit (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... a 6x stock solution of the beta-lactamase substrate CCF2-AM (Thermo Fisher, K1032) was added to the media over 10 T1/2 cells so that the final concentration of CCF2-AM was 1x ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated in beta actin primary antibody (1:1000, Thermo Fisher Scientific, Cat. #MA515739) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... mouse monoclonal beta-catenin Alexa Fluor 647-conjugated antibody (Thermo Fisher Scientific; 1:100); mouse monoclonal VE-cadherin Alexa Fluor 488-conjugated antibody (Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Each reaction was normalised to a beta-2-microglobulin (B2M) control (Hs00984230_m1; Applied Biosystems). For quantifications using SYBR-green ...
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bioRxiv - Synthetic Biology 2020Quote: ... 3.3 nM of 5-chloromethylfluoresecein di-beta-D-galactopyranoside (CMFDG) (Invitrogen, Waltham, MA, USA) was added to the reaction solution ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies include Anti-beta tubulin (1:500, mouse monoclonal, 2 28 33, Invitrogen), Anti-Hec1 (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... and beta Actin mouse Monoclonal Antibody (AC-15) (# Cat. No. MA1-91399, Thermo Scientific). The secondary antibodies used were Anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: Primary pancreatic beta cells were dispersed from the purified islets using trypsin (GIBCO, 25200072) digestion and cultured in RPMI 1640 medium (GIBCO ...
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bioRxiv - Genetics 2023Quote: ... 0.1 mM beta-mercaptoethanol,100 U/ml penicillin and 100 mg/ml streptomycin (Gibco)) on SNL-HBP feeder cells ...
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bioRxiv - Biophysics 2023Quote: Primary antibodies used were: Mouse-beta tubulin monoclonal antibody (Thermo Fisher Scientific, 32–2600), Rabbit-anti-Tom20 polyclonal antibody (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated overnight with primary antibody directed against Aβ (Invitrogen, beta Amyloid (1-40) Polyclonal Antibody ...
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bioRxiv - Biophysics 2024Quote: ... Primary antibodies include Anti-beta tubulin (1:500, mouse monoclonal, 2 28 33, Invitrogen) and Anti-Hec1 (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... AM (#F1225) and the anti-beta-actin BA3R (#MAS-15739) antibody was from ThermoFisher Scientific.
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bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Purified recombinant plasmid DNA was digested with PacI and transfected into HEK293 cells with Lipofectamine (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The fibroblasts and HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (#11965–092; Gibco, NY) supplemented with 10% FBS (#12483-020 ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells (ATCC Cat# CRL-1573, RRID:CVCL_0045) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa and HEK293 cells were cultured in DMEM with sodium pyruvate and GlutaMAX (Life Technologies 10569044) or 2 mM L-glutamine supplemented with 10% heat-inactivated fetal bovine serum (Sigma F4135 ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were subjected to drug selection in the medium with 2 μg/ml puromicin (Invitrogen) for 14 days ...
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bioRxiv - Systems Biology 2020Quote: Flp-In™ T-Rex™ HEK293 cells (Life Technologies, we did not test for mycoplasm) were cultured following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... expressing mouse ATG16L1 variants was transfected into HEK293 ATG13_KO GFP-LC3B cells via Lipofectamine 2000 (Invitrogen). Cells were selected with 2.5 μg/ml puromycin (P8833 ...
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bioRxiv - Biophysics 2020Quote: ... HEK293 cells were transfected (in 35-mm-diameter wells) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) and plated onto coverslips coated with Matrigel (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: BiP-3xFLAG HEK293 cells (0.12 × 106) were plated on 12 mm round glass coverslips (Fisher Scientific) coated with 0.15 mg/mL poly-lysine in 24-well plates ...
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bioRxiv - Plant Biology 2022Quote: HEK293 cells were transfected with the respective eYFP-TPC1-fused constructs with lipofectamine2000 (ThermoFisher, Waltham, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... siRNA duplexes (siRNA B and C) were transfected into HEK293-PSMB2-HB cells using RNAiMAX (Invitrogen) dissolved in Opti-MEM (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: HEK293 (ATCC) were transfected using Lipofectamine LTX® Reagent and OptiMEM Reduced Serum Medium (Thermo Fisher) with pCMV-MAPK14 Wt (DU1765 ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293 cells were transfected with relevant plasmids or siRNA using Lipofectamine 2000 reagent (Thermo Fisher Scientific), and harvested 48 h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... B(-)S13−) bacmids were transfected into HEK293 cells using Neon transfection system (Invitrogen, Carlsbad, CA, USA). 5 x 104 cells were resuspended in 100 μl of serum-free media containing 5ug of each mutant recombinant bacmid ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were rinsed with cold PBS then scraped in PBS+1xHALT protease/phosphatase inhibitor (ThermoFisher). The cell slurry was centrifuged for 5 min at 1,000xg at 4°C to pellet cells ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 cells were seeded at 2 ×105 cells per well in six well plates (Thermo Fisher) in DMEM/10% FBS ...
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bioRxiv - Genetics 2022Quote: ... HEK293 cell cultures were transfected with 15 μg of the plasmid DNA using Lipofectamine 2000 (Invitrogen). Transfected cells were harvested 24 hours after transfection and total RNA was isolated using RNeasy mini kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: HEK293 and A549 cells were transfected with 15 pmol of siRNA using Lipofectamine RNAiMAX (Life Technologies). siRNAs (Dharmacon ...