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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade D UG28R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Clarified lysates were incubated with 1 ug of YB-1 antibody and antibody/protein/RNA complexes were isolated by using 20 μl packed volume protein A sepharose beads (Invitrogen). Beads were washed with polysome extraction buffer and RNA isolated using the PeqGold RNA isolation kit ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein pellets were resuspended in 1% SDS and protein concentration was measured using the Bradford assay kit (Thermo Scientific, 1863028), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... which were pre-conjugated to 1:1 mix of 63 µL protein A/G dynabeads (Invitrogen), and incubated (4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... HeLa cells in 35-mm dishes were transfected with 100 pmol of each siRNA in the Stealth siRNA library targeting 154 human nuclear proteins (Invitrogen–Thermo Fisher Scientific) using Lipofectamine RNAiMax (Invitrogen–Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: Cell culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel for MAGPIX (Thermofisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Cell culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel for MAGPIX (Thermofisher Scientific, Carlsbad, CA) as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: Epidermal growth factor receptor (EGFR) protein expression levels on the surface of Gli36-derived EVs were quantified using an EGFR Human ELISA kit (ThermoFisher Scientific, Waltham, MA). EVs were spiked in healthy donor serum at different concentrations ranging from 0 to 1.0E11 particles/mL while maintaining the serum-derived EV concentration at 1.0E9 particles/mL ...
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bioRxiv - Bioengineering 2023Quote: ESCs were maintained on Recombinant Human Protein Vitronectin (Thermo Fischer # A14700) coated plates using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001) ...
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bioRxiv - Immunology 2023Quote: To assess thermodynamic stability of human and bat (M. myotis and M. capaccinii) purified IgG molecules we used Protein Thermal Shift Dye Kit (Applied Biosystems, Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... were determined by matching the UniProt human protein database (release 2023_01) with the acquired fragmentation pattern using Sequest (Thermo Fisher Scientific, Waltham, MA) (Eng et al. ...
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bioRxiv - Neuroscience 2020Quote: ... and combined with 50μl 1× D-Luciferin Working Solution supplemented with 1× Firefly Signal Enhancer (Thermo Scientific Pierce Firefly Signal Enhancer; Thermo Scientific 16180). Following a 10 min dark incubation period to allow for signal stabilization ...
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bioRxiv - Molecular Biology 2021Quote: ... and shINTS11 cells were seeded at a density of 1 × 106 cells in 10 cm dishes at d-1 in DMEM medium (Gibco, #11965-084), supplemented with 10% FBS (Atlas Biologicals ...
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bioRxiv - Cell Biology 2020Quote: Naïve mouse ESCs and D-EPSCs were cultured in a base medium of N2B27 prepared as follows: 1:1 ratio of DMEM/F12 (ThermoFisher Scientific 21331020) and Neurobasal (ThermoFisher Scientific 21103049) ...
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bioRxiv - Molecular Biology 2023Quote: ... and lungs were dissected and placed in ice cold FACS buffer (1% BSA, 3% FBS, 96% Ca2+ and Mg2+ free PBS) with 1 μg/ml Actinomycin D (ThermoFisher BP606-5). After isolation ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-APP (22C11, 1:1000 dilution), Anti-β Amyloid (D-11 Santacruz, Cat. Sc-374527, 1:1000) and anti-β-tubulin (Invitrogen Cat.32–2600 or Proteintech Europe ...
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bioRxiv - Synthetic Biology 2024Quote: ... HEK293FT and HEK293FT-LP cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco 25300-054). All HEK293FT cell lines generated by engineering either the HEK293FT or HEK293FT-LP parent cell lines were cultured in the same way ...
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bioRxiv - Cancer Biology 2024Quote: ... The membrane was then blocked using 5% w/v non-fat dry milk in TBST for one hour at room temperature and then incubated with primary antibody (anti-Alix, 1:5000; Protein Tech, 12422-1-AP or anA-FloAllin-1, 1:5000; Fisher Scientific, BDB610820) at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Lentiviral vector CSII-CMV-MCS-IRES2-Bsd harboring a cDNA was transfected with the packaging vectors pCAG-HIV-gp and pCMV-VSV-G-RSV-Rev into X293T cells using Lipofectamine2000 transfection reagent (Life Technologies/Thermo Fisher Scientific, Carlsbad, CA, USA). To generate S2-CP8 stable cells above ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured in complete DMEM (4.5 g/L D-Glucose, L-Glutamine, 1% penicillin/streptomycin, Gibco) supplemented with 10% FBS (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and 100 μM D-luciferin) for 1 hour then cycloheximide (CHX) (Fisher Scientific, Hampton, NH, cat # 50255724) was added at a final concentration of 100μg/ml diluted in serum free media.
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bioRxiv - Immunology 2022Quote: ... total RNA was prepared using lysing matrix D (MP Biomedical) containing 1 mL of TRIzol reagent (ThermoFisher) and homogenization at 30 s at 6.0 m/s twice using MP Biomedical Fastprep 24 Tissue Homogenizer ...
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bioRxiv - Microbiology 2021Quote: ... Tissues were incubated with primary antibodies (mouse anti-HuC/D IgG clone 16A11 at 1:200 [Thermofisher] ...
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bioRxiv - Neuroscience 2020Quote: ... Transfected HEK293 cells were solubilized in 0.1-1% (w/v) n-dodecyl-β-D-maltoside (ThermoFisher Scientific), 1 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2019Quote: ... at which point it was induced with 0.4 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, ThermoFisher Scientific) and grown overnight at 200 rpm at 20°C ...
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bioRxiv - Plant Biology 2022Quote: The coding sequence of MpHYP without the stop codon was amplified from Tak-1 cDNA using the primer set MpHEC1_CDS_F and MpHEC1_CDS_nsR and cloned into pENTR/D-TOPO (Thermo Fisher), followed by an LR reaction with pMpGWB313 (Ishizaki et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... The embryos were then permeabilized by 1 min incubation in EPS solution (90% D[+] Limonene [Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed thrice for 5 min in PBS before incubation with Alexa 594 goat-anti rabbit secondary antibody at 1:1,000 and Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... EpiLCs were induced by plating 1.5 x 106 ESCs on a 10 cm dish coated with human plasma fibronectin (16.7 mg/ml) in the 1:1 mix of DMEM/F12 (Gibco, 11302-33) and Neurobasal medium containing N2 supplements ...
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bioRxiv - Evolutionary Biology 2020Quote: ... a formulation optimal for human fibroblasts [(Dickson et al., 2000): 1:1 (vol/vol) medium M199 and medium M106 (ThermoFisher Scientific) + 15% BCS + 10 ng/ml EGF + 0.4 ug/ml hydrocortisone + 1% Penicillin-Streptomycin] ...
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bioRxiv - Microbiology 2023Quote: ... and was then incubated (1:1000 dilution) for 1 hour with a mouse anti-human E-Cad ectodomain antibody (HECD-131700, 1:1000 dilution; Life Technologies). After two washes ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used in this study were all anti-human: STING (1:1000, CST, #13647 or 1:500, Thermo Fisher, MA526030), UBIQUITIN (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at room temperature and then exposed to antibodies specific for human PD-1 (ThermoFisher Scientific; 14-9969-82), Iba1 (Wako Chemicals ...
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bioRxiv - Bioengineering 2023Quote: ... primary T cells were activated and stimulated with a 1:1 ratio of anti-human CD3/CD28 magnetic Dynabeads (Thermo Fisher) to cells in X-vivo 15 medium supplemented with 5% human AB serum (Heat inactivated) ...
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bioRxiv - Biophysics 2024Quote: ... primary stained with goat anti-Syndecan-1 antibody and mouse anti-human choriogonadotropin (β-hCG) antibody (#14-6508-82, 1:500 dilution in the buffer solution, ThermoFisher), and secondary stained as previously described ...
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bioRxiv - Plant Biology 2019Quote: ... and cloned into pENTR-D-TOPO vector to give pENTR-D-GUN1 (Thermo Fisher, Tokyo, Japan). p511G1pro was digested with XhoI ...
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bioRxiv - Developmental Biology 2021Quote: ... full-length open reading frame sequences coding for SlCRCa and SlCRCb proteins were cloned into the pENTR/D-TOPO vector (Invitrogen Life Technologies, San Diego, CA, USA) and recombined into the Gateway binary vector pGWB6 (Nakagawa et al. ...
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bioRxiv - Neuroscience 2021Quote: ... frozen EVs were resuspended in RLT lysis buffer and RNAse-free water in a ratio of 1:3 and loaded based on protein concentration measured by Micro BCA Protein Assay Kit (Thermo Scientific). 2,8 μg of proteins were loaded.
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bioRxiv - Cancer Biology 2022Quote: Cells were lysed in 1% NP-40 lysis buffer (58) and protein levels were quantified using the Bicinchoninic acid protein assay (Thermo Scientific). Samples were run on SDS-PAGE gels before being transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... Lysates from 30 wt eye imaginal discs (1 mg of total protein) was precleared by incubating with protein A-sepharose beads (Thermo Scientific) for 1h at 4 °C followed by centrifugation ...
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bioRxiv - Biochemistry 2022Quote: ... and 150 µg of purified PPSA from SG and SEC fractions at 1 mg/mL total protein concentration (measured by a Pierce BCA protein assay, Thermo Scientific, according to manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were incubated with a 1:200 dilution of human cTnT primary mouse antibody (ThermoFisher) overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with a PE-conjugated anti-human IgG1(Fc) secondary antibody (1:50) (ThermoFisher) for additional 15min ...
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bioRxiv - Neuroscience 2019Quote: ... or 10 μM of freshly prepared human synthetic amyloid-β peptide 1-42 (Aβ42, Life technologies) for 24 h as described (Kiyota et al. ...
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bioRxiv - Microbiology 2020Quote: The human acute leukemia monocyte cell line (THP-1) was cultivated in RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated FBS (Thermo Scientific Hyclone ...
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bioRxiv - Biochemistry 2022Quote: ... The ORF of human MYBL2/B-Myb isoform 1 (NM_002466.4) was cloned into pcDNA3.1+ (ThermoFisher Scientific) and fused with an N-terminal Flag tag ...