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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Variants of those MDCK cells with increased levels of α2,6-linked Sia cells were prepared in the laboratory by transfection with the ST6Gal1 gene in a plasmid under the control of the CMV promoter (pcDNA3.1, Invitrogen). Cell clones with increased levels of α2,6-linked Sia were identified by staining with the Sambucus nigra (SNA ...
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bioRxiv - Physiology 2019Quote: ... and a non-targeting pre-miRNA under bicistronic control of the CMV promoter in the pcDNA6.2GW/EmGFP-miR plasmid (Invitrogen). The UBR5 RNAi plasmid encodes EmGFP and an artificial pre-miRNA targeting mouse UBR5 under bicistronic control of the CMV promoter ...
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bioRxiv - Physiology 2020Quote: ... and a non-targeting pre-miRNA under bicistronic control of the CMV promoter in the pcDNA6.2GW/EmGFP-miR plasmid (Invitrogen). The UBR5 RNAi plasmid also encodes EmGFP plus an artificial pre-miRNA targeting mouse UBR5 under bicistronic control of the CMV promoter ...
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bioRxiv - Molecular Biology 2020Quote: ... pAAV-CMV-eGFP3 was co-transfected into PC3 cells with TRIBE construct using Lipofectamine™ 2000 (Thermo Fisher Scientific). One day or two days later ...
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bioRxiv - Evolutionary Biology 2019Quote: ... or HDHD-NDA-RA-pGL4.27 along with 500 ng pRL-CMV (Renilla reporter plasmid) via electroporation (Neon Transfection System, Invitrogen). Firefly and Renilla luciferase activity were analyzed using the Dual-Glo Luciferase Assay System (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... Transfections of transfer vector (pLentiCRISPRv2 SCRA, pLentiCRISPRv2 LAMA5) and packaging plasmids (CMV-VSVg and Delta8.9) were performed using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Successful incorporation of pShuttle-CMV construct into AdEasier-1 cells confirmed via digestion with PacI (Thermo Fisher Scientific, IVGN0184). 5000 ng plasmid was then digested at 37°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... For overexpression of miR-7704 the MDA-MB-231 cells were grown in six-well plates and transfected for 48h with pPRIME-miR-7704-CMV-GFP-FF3 plasmid (5.5 µg/well) using Lipofectamine-2000 reagent (ThermoFisher). As control ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pAAV-CMV-tKiMBI-T2A-caMEK-bGH_pA plasmids were purified by PureLink Expi Endotoxin-free Maxi plasmid Purification kit (Invitrogen), and then were sent to Stanford Gene Vector and Virus Core to produce and titer AAV.DJ vectors.
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bioRxiv - Cell Biology 2024Quote: ... 50 ng of pRL-CMV renilla vector or 1μg of pGL3 empty in 24-well plates using Lipofectamine LTX plus (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... The fragments were digested in digestion medium (advanced DMEM/F12 with 2% FBS, Pen/Strep, 100 U/mL collagenase type XI [SigmaAldrich], and 125 μg/mL dispase type II [Invitrogen, Waltham, MA, USA]) at 37 °C for 40 min and then further digested for 10 min by adding a half-volume of TrypLE Express ...
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bioRxiv - Biophysics 2019Quote: ... To calibrate the point spread function 100 nm and 50 nm fluorescent particles (ThermoFisher Scientific) were used ...
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bioRxiv - Cancer Biology 2020Quote: Lentiviral particles were produced in human 293T cultured in in Dulbecco’s Modified Eagle’s Medium (ThermoFisher), supplemented with 10% Fetal Bovine Serum (EuroBio ...
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bioRxiv - Microbiology 2021Quote: ... and the number of virus particles was determined using a Nanodrop 2000 (Thermo Fisher Scientific). The adenovirus stocks were aliquoted and stored at -80°C.
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: HIV-1 particle production was carried out using Lipofectamine 2000 transfection reagent (Invitrogen, Life Technologies), according to the manufacturer’s protocol ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: HIV-1 particle production was carried out using Lipofectamine 2000 transfection reagent (Invitrogen, Life Technologies), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: Lentiviral particles for pCDH vectors were produced using third generation ViraPower Lentiviral Packaging Mix (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Native kinetochore particles were purified (see kinetochore purification assay) and kept bound to Dynabeads (Invitrogen). Beads were washed once with phosphatase buffer (Buffer H ...
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bioRxiv - Molecular Biology 2022Quote: ... Images of VSV particles were collected on a Titan Krios G3i microscope (Thermo Fisher Scientific) operated at 300 kV ...
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bioRxiv - Immunology 2022Quote: ... Single-particle cryo-EM data were collected on a Glacios transmission electron microscope (Thermo Fisher) operating at 200 kV ...
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bioRxiv - Biochemistry 2022Quote: ... Data acquisition was performed with EPU Automated Data Acquisition Software for Single Particle Analysis (ThermoFisher) with three shots per hole at −1.0 μm to −2.4 μm defocus (0.2 μm steps).
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bioRxiv - Biochemistry 2022Quote: ... Data acquisition was performed with EPU Automated Data Acquisition Software for Single Particle Analysis (ThermoFisher) with three shots per hole at −1.0 μm to −2.4 μm defocus (0.2 μm steps).
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bioRxiv - Biophysics 2022Quote: ... Data acquisition was performed with EPU Automated Data Acquisition Software for Single Particle Analysis (ThermoFisher) with three shots per hole at -0.50 µm to -4.0 µm defocus for all samples.
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bioRxiv - Molecular Biology 2022Quote: ... Data acquisition was performed with EPU Automated Data Acquisition Software for Single Particle Analysis (ThermoFisher) with three shots per hole at -1.3 µm to -3.5 µm defocus.
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bioRxiv - Microbiology 2021Quote: ... PEG precipitated phage particles were incubated with Sybr Green (Invitrogen, Molecular probes Cat. no. S7563) at 80°C for 10 minutes in 10−4 final dilution of commercial stock as described earlier (35 ...
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bioRxiv - Microbiology 2021Quote: ... These particles were first coated with the pH-insensitive dye Alexa Fluor-647 (Life Technologies) for 1 h at 37°C in 0.1M sodium tetra borate decahydrate (pH ...
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bioRxiv - Biophysics 2020Quote: ... Therefor fluorescent marker stock supension (0.1 µm, ∼1.8 × 1011 particles/mL, TetraSpeck Microspheres, Thermo Fisher) was vortexed for ∼1 minute and then diluted 1:50 in the corresponding monomer solution ...
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bioRxiv - Evolutionary Biology 2022Quote: ... where an Acclaim PepMap 100 (2 cm x 75 μm, 3 μm particles; Thermo Fisher) pre-column was connected in front of an EASY-Spray PepMap RSLC C18 reversed phase column (50 cm x 75 μm ...
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bioRxiv - Immunology 2022Quote: Single-particle cryo-EM data were collected on a Talos Arctica transmission electron microscope (ThermoFisher) operating at 200 kV ...
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bioRxiv - Molecular Biology 2019Quote: ... an Acclaim PepMap 100 (2 cm x 75 μm, 3 μm particles, Thermo Fisher Scientific) pre-column was connected in front of an EASY-Spray PepMap RSLC C18 reversed phase column (50 cm x 75 μm ...
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bioRxiv - Biochemistry 2019Quote: ... serving as reference particles and 2.8 μm streptavidin-coated magnetic beads (Life Technologies, Darmstadt, Germany) with prebound DNA molecules were flushed into the flowcell ...
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bioRxiv - Microbiology 2020Quote: ... Exo samples (1 x 109 particles/mL) and RNase (20 µg/mL) (Thermo Fisher Scientific) were incubated with 0.1 mg/ml saponin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Data acquisition was performed with EPU Automated Data Acquisition Software for Single Particle Analysis (ThermoFisher) at −0.7 µm to −2.5 µm defocus.
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bioRxiv - Cancer Biology 2019Quote: ... The pGIPZ lentiviral particles were generated by transfection of 293Ts using Lipofectamine 2000 (ThermoFisher Scientific), per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... packed with 2 μm particles with a pore size of 100 Å (Thermo Fisher Scientific), applying a linear gradient from 2% to 40% solvent B (80% acetonitrile ...
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bioRxiv - Biochemistry 2021Quote: ... Data acquisition was performed with EPU Automated Data Acquisition Software for Single Particle Analysis (ThermoFisher) with three shots per hole at −1.0 μm to −2.4 μm defocus (0.2 μm steps).
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bioRxiv - Cell Biology 2021Quote: Images for single-particle analysis were collected using a Lab6 G2 Tecnai microscope (Thermo Scientific) operated at an acceleration voltage of 200 kV ...
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bioRxiv - Immunology 2020Quote: ... For separation of peptides Hypersil gold C18 (100x2.1 mm; particle size: 1.9 µm, ThermoFisher, USA) column was used ...
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bioRxiv - Microbiology 2021Quote: ... PEG precipitated phage particles were incubated with Sybr Green (Invitrogen, Molecular probes Cat. no. S7563) at 80°C for 10 minutes in 10−4 final dilution of commercial stock as described earlier (35 ...
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bioRxiv - Cancer Biology 2022Quote: CD11b+ cells were incubated with AlexaFluor® (AF)488-coupled acLDL particles (Invitrogen, Karlsruhe, Germany) diluted 1:500 in culture medium for 4 hours at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... were used to assemble lentiviral particles using HEK293T cells via transfection using TurboFect Reagent (Invitrogen). Transduction of mES cells was performed overnight by addition of lentivirus to culture medium and washed the following day (Moris et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Lentiviral particles were generated by first transfecting 1 million HEK293FT with Lipofectamine 2000 (Thermo Fisher) according to manufacturer’s protocols using 2.5 µg plasmid DNA ...
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bioRxiv - Cell Biology 2024Quote: ... Submicron Particle Size Reference Beads (0.5 µm, 1 µm and 2 µm, Thermo Fisher Scientific) were also used to identify mitochondria.
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bioRxiv - Molecular Biology 2023Quote: ... and the particles remaining on the filter were stained with SYBR Gold (Life Technologies, USA), mounted on slides ...
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bioRxiv - Systems Biology 2023Quote: ... The particle eluates were pooled and concentrated by 100-kDa cutoff centrifugal filtration (Thermo Scientific), resulting in the final volume of 1 mL ...
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bioRxiv - Biochemistry 2023Quote: ... Particles were imaged in a Titan Krios G3i Cryo Transmission Electron Microscope (Thermo Fisher Scientific) at 300 kV with a K2 Summit direct electron detector camera with GIF Quantum energy filter (Gatan) ...
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bioRxiv - Bioengineering 2023Quote: ... we blocked the particle and plate surface using a protein-free blocking buffer (ThermoFisher Scientific) for 1 hour.
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bioRxiv - Microbiology 2023Quote: RNA was extracted from cells and viral particles using the Trizol Reagent (ThermoFisher Scientific, USA) and quantified using a Nanodrop spectrophotometer ...
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bioRxiv - Immunology 2023Quote: ... approximately 1×1013 phage particles were incubated in 1 ml SuperBlock™ (Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Blockade of β-actin: differentiated neurons and primary neurons were treated with 2µg of either anti β-actin (mouse IgG1) Antibody (Thermo Fisher Scientific), or normal mouse IgG1(Abcam ...