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Citations for Thermo Fisher :
1201 - 1250 of 6996 citations for sCD4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: The HEK293 cells (CRL-1573, ATCC, USA) were kept in the Dulbecco’s Modified Eagle Medium (High Glucose)-DMEM (Invitrogen, USA) supplemented with 10% fetal bovine serum-FBS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 Phoenix cells (National Gene Vector Biorepository, Indianapolis, IN) were transfected with each construct using Lipofectamine 3000 (Thermo Fisher Scientific) per manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2019Quote: ... Transient transfection of 2N3T-Tip60 or the corresponding control empty vector was carried out for 48 hours into HEK293 cells using Lipofectamine 2000 transfection reagent (Invitrogen); UV irradiation was carried out at a dose of 15mJ/cm2 using Stratagene Stratalinker 1800 ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells (ATCC Cat# CRL-1573) were maintained at 37° C and 5% CO2 in 1X DMEM medium (Thermo Scientific; Waltham ...
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bioRxiv - Molecular Biology 2019Quote: Expression constructs for repeat RNAs or GFP-tagged codon-optimized DPRs were transfected in HEK293 cells or U-2 OS cells with Lipofectamine 2000 (Invitrogen) or FuGENE HD (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... pEGFP-C2-IQGAP1 and pcDNA3/RH-p122RhoGAP/DLC-1 plasmids were transfected into HEK293 cells using Lipofectamine 3000 (Invitrogen, MA).
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bioRxiv - Microbiology 2021Quote: ... Plasmids encoding the SARS-COV-2 spike glycoprotein or its mutated variants were cloned into a G418 resistance gene containing backbone and transfected into HEK293 cells using Lipofectamine 2000 (Thermofisher) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... P2 or P3 baculovirus was produced in Sf9 cells and used to transfect HEK293S GnTI- suspension cells cultured in Freestyle 293 (Gibco) with 2% FBS at 37°C with 8% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... The inter-molecular (oligomer) and intra-molecular (monomer-conformation) aSyn FRET biosensors were generated by transiently transfecting HEK293 cells using Lipofectamine 3000 (Invitrogen) with GFP-aSyn and aSyn-RFP (1:8 DNA plasmid concentration ratio ...
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bioRxiv - Systems Biology 2021Quote: ... HEK293 and Ptk2 cell lines were cultured in DMEM (high glucose, pyruvate) supplemented with 10 % FBS and antibiotic-antimycotic (Invitrogen) and passaged routinely ...
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bioRxiv - Systems Biology 2020Quote: HEK293 cell lines 293-F and Freestyle 293-F were cultivated in Freestyle 293 expression medium (Gibco, Thermo Fisher Scientific) at 37°C ...
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bioRxiv - Systems Biology 2020Quote: HEK293 cell lines 293-F and Freestyle 293-F were cultivated in Freestyle 293 expression medium (Gibco, Thermo Fisher Scientific) at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells that express mNRK in a dox-dependent manner were developed using Flp-In T-REx 293 cells (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... pX330 plasmid harboring a p53-specific gRNA (Supplementary Table 1) was transfected into HEK293 and HCT116 cells using Lipofectamine 2000 (Invitrogen). Two days later ...
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bioRxiv - Molecular Biology 2022Quote: Inducible HEK293 cell lines expressing mt-ELP3 were generated by using the Flp-In T-REx Core Kit from Invitrogen. Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the IFN-β–Luc was co-transfected with pRL-TK in wild type and 4EHP-KO HEK293 cells using Lipofectamine 2000 according to the manufacturer′s protocol (Invitrogen). 24 h after transfection ...
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bioRxiv - Immunology 2022Quote: Binding to P-selectin of the A48 and D48 variants of PTX3 from HEK293 cells was then assessed using 96 well Maxisorp plates (Nunc) coated with a recombinant form of the human P-selectin ectodomain (spanning the 42-771 sequence of the preprotein ...
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bioRxiv - Cell Biology 2019Quote: ... Stable tetracycline inducible Flp-In T-REx HEK293 cell lines were generated using the T-Rex system (Thermo Fisher Scientific) as previously described (Kang et al. ...
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bioRxiv - Cancer Biology 2019Quote: ∼1 x 106 HEK293 cells grown at 70% confluency on 35 mm dish embedded with 12 mm glass viewing area (Nunc, Thermo Fisher Scientific Inc. ...
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bioRxiv - Developmental Biology 2019Quote: HEK293 cells were passaged in HyClone DMEM (GE) supplemented with 5% FBS and 100 units pen/strep per ml (Gibco) at 37°C and 5% CO2 under humidified conditions ...
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bioRxiv - Biochemistry 2019Quote: ... 1.5 μg psPAX2 packaging plasmid and 500 ng pMD2.G envelope plasmid were co-transfected in HEK293 FT cells in 10-cm dishes using Lipofectamine (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293-B3GALT4 KO and HEK293-B3GALT4-PIGZ DKO cells stably expressing HFGF-CD59 were treated with 0.5 unit/mL PI-PLC (Thermo Scientific) in 5 mL of PI-PLC buffer (Opti-MEM containing 10 mM HEPES-NaOH (pH 7.4) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: African green monkey kidney epithelial cells (Vero, ATCC) and HEK293 T cells (ATCC) were cultured in DMEM containing 10% fetal bovine serum (FBS, Gibco Invitrogen) at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 Flp-In T-Rex cells were maintained in DMEM supplemented with 10% tetracyclin-free fetal bovine serum (Gibco, 10082139) and 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... 15 × 104 wildtype HEK293 cells or SMAP1 KO A2 cells were transfected with GFP-vWF with Lipofectamine LTX (Thermo Fisher) by reverse transfection ...
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bioRxiv - Physiology 2020Quote: HEK293 cells were purchased from ATCC (Manassas, VA) and incubated in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 and CHO cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F-12; Gibco, #11320033), supplemented with 10% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Transfections were carried out using 1 mg of total DNA (500 μg each of VH and Vκ plasmid DNA) and 1 l of cultured HEK293-F cell suspension maintained in sterile Freestyle 293 expression medium (Invitrogen) without antibiotics at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... The mixtures were kept still for 10 min and then added separately to a 24-well plate with each well containing 1mL of 106/mL HEK293-Freestyle cells (Invitrogen). The transfected cells were cultured by shaking at a speed of 200 rpm at 37 °C for 72 h followed the transfection ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were seeded at ∼10-15 % confluency 48 hours before a triple transfection with Lipofectamine 2000 (Thermo Fisher Scientific) as per the manufacturers protocol.
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bioRxiv - Systems Biology 2021Quote: ... HEK293 transfected cells were stained with Hoechst 3342 nuclear stain and a wheat germ agglutinin (WGA, Invitrogen Alexa Fluor 647) plasma membrane (PM ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293 cells were obtained from ATCC (CRL-1573) and cultured in DMEM with Glutamax and 25 mM glucose (Life Technologies) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2021Quote: HEK293 HeLa and M17 cells were cultured at 95% air and 5% CO2 in DMEM (for HEK293 and HeLa) or DMEM-F12 (1:1; for M17) supplemented with 10% fetal bovine serum (Gibco) and penicillin-streptomycin (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: HEK293 were transfected with 3xFlag-tagged plasmids encoding each SARS-CoV-2 protein by Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015). 3×107 HEK293 cells were harvested in 10 ml lysis buffer (50 mM Tris-HCl [pH 7.5] ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... The lentivirus of gRNA or shRNA library was produced by co-transfection of library plasmids with two viral packaging plasmids psPAX and pMD2.G into HEK293 cells using Lipofactamine 2000 (Invitrogen). Huh7 cells were transduced with lentivirus libraries at multiplicity of infection (MOI ...
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bioRxiv - Microbiology 2020Quote: ... and HEK293S GnTI-/- cells (ATCC CRL-3022) in Freestyle 293 Expression Medium supplemented with 2% ultra-low IgG FBS (Gibco). We maintained ...
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable and HEK293S GnTI-cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2022Quote: HEK293 cells were obtained from ATCC (CRL-1573) and grown in DMEM (high glucose, L-glutamine, no sodium pyruvate) (ThermoFisher), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2023Quote: ... non-transfected HEK293 cells were labeled with the CFSE CellTrace and the Soromba expressing cells were labeled with Violet CellTrace (Invitrogen). After washing ...
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bioRxiv - Biochemistry 2024Quote: ... plasmid DNA into 1 × 106 HEK293 cells plated per well of a 6-well plate using Lipofectamine 2000 transfection reagent (Invitrogen). Each well was transfected with 2 µg of total plasmid DNA (50 ng Cg4-puro ...
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bioRxiv - Neuroscience 2024Quote: ... ASAP3 construct was cloned in pcDNA3.1/Puro-CAG plasmid backbone and used to transfect HEK293 cells with Lipofectamine 3000 (ThermoFisher, US) using 400 ng DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... fused to a C-terminal trimeric GCN4 (tGNC4) zipper tag via a 5XGGS linker region was secreted from suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
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bioRxiv - Molecular Biology 2023Quote: ... The 3’ ends of the sperm and HEK293 TFAM mRNA species were mapped by 3’ RLM-RACE using the FirstChoice RLM-RACE Kit (Ambion) with modifications ...
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bioRxiv - Biochemistry 2023Quote: ... HeLa (ATCC CCL-2) and HEK293 (ATCC CRL-1573) cell lines were maintained in Dulbecco’s Modified Eagle Medium (Invitrogen 11965092) supplemented with 10% fetal bovine serum (Invitrogen 16140071) ...
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen). After 6-8h transfection ...
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bioRxiv - Microbiology 2023Quote: ... suspension-adapted and serum-free HEK293S cells were seeded at 3 × 106 cells/mL in Freestyle-293 medium (ThermoFisher Scientific). Next ...
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bioRxiv - Cell Biology 2023Quote: ... Lentivirus was generated according to the manufacturer’s specifications by co-transfecting HEK293-FT’s with a mixture of packaging plasmids (ViraPower Lentivirus Expression System; ThermoFisher #K497500). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... To generate stable expression cell lines Flp-In T-Rex HEK293 were grown in 100 μg/ml Zeocin (Invitrogen, R25001) for at least a week before transfection ...
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bioRxiv - Biochemistry 2023Quote: ... PANX1 and its mutants and PANX3 were transiently transfected into HEK293 cells with an enhanced green fluorescent protein (eGFP) using the transfection agent Lipofectamine 2000 (Invitrogen). The cells expressing GFP were selected for patch clamp electrophysiological recordings after 24-36 hours of transfection.
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen) as described previously (31) ...