Labshake search
Citations for Thermo Fisher :
1151 - 1200 of 6996 citations for sCD4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2020Quote: ... rabbit monoclonal anti-6x-His tag at 1:1,000 (Thermo Fisher Scientific, MA5-33032), and mouse monoclonal anti-β-actin at 1:10,000 (R&D Systems ...
-
bioRxiv - Molecular Biology 2021Quote: ... The cleared lysate was incubated with 1mL His-Pur nickel-NTA resin (Thermo Fisher) with rotation at 4 °C for 1 hour ...
-
bioRxiv - Molecular Biology 2020Quote: ... ZAP-S and ZAP-L were subcloned into pEF1-V5/His (Thermo Fisher Scientific) via the KpnI/XbaI sites to generate pEF1-ZAP-S-V5/His and pEF1-ZAP-L-V5/His ...
-
bioRxiv - Neuroscience 2022Quote: ... the pEx-FGD4-His-V5 vector was generated by Gateway cloning technology (Thermofisher, USA). Transfection experiments were performed using promofectin reagent (#PK-CT-2000-50 ...
-
bioRxiv - Synthetic Biology 2022Quote: All antigens were His-tagged and purified using HisPur™ Ni-NTA resin (ThermoFisher). Cell supernatants were diluted with 1/3rd volume wash buffer [20 mM imidazole ...
-
bioRxiv - Microbiology 2022Quote: ... or membrane probed with mouse monoclonal 6x-His tag antibody (4A12E4) (Thermofisher, Waltham, MA) (Supplementary Figure 2).
-
bioRxiv - Immunology 2022Quote: ... unbiotinylated form were incubated with 2μg/mL anti-His biotin (Invitrogen, MA1-21315-BTIN) for 20 min at room temperature before being used to label the streptavidin beads ...
-
Assessment of Human Renal Transporter Based Drug-Drug Interactions Using Proximal Tubule Kidney-ChipbioRxiv - Cell Biology 2022Quote: ... Heat inactivated fetal bovine serum (HI FBS) and trypan blue were procured from Gibco Life Technologies (Waltham ...
-
bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Life Technologies unless indicated), 100 U/mL penicillin-streptomycin ...
-
bioRxiv - Microbiology 2020Quote: ... 3 μg of mammalian expression vector plasmids based on pEF1α V5 His C (Invitrogen) encoding codon-optimized gH ...
-
bioRxiv - Microbiology 2019Quote: Qst was fused to a His-tag using the pEXP5-CT/TOPO vector (Invitrogen) following the TA-cloning protocol provided by the manufacturer ...
-
bioRxiv - Immunology 2019Quote: ... 1xPen/Strep/Glu and 10% ultralow IgG HI-FBS (Thermo Fisher Scientific, Waltham, MA), and pelleted at 350xg for 5min ...
-
bioRxiv - Biochemistry 2019Quote: ... A full length cDNA of Matriptase constructed in pcDNA3.1-V5-His (Thermo Fisher Scientific) was provided by Dr ...
-
bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified using HisPur Ni-NTA resin (Thermo Fisher Scientific), at RT ...
-
bioRxiv - Molecular Biology 2021Quote: ... Then the blot was incubated with HRP conjugated anti-His antibody (Invitrogen, LOT 1902132) at 1:5000 dilutions for 16h at 40 C ...
-
bioRxiv - Immunology 2021Quote: ... and the protein was purified on His-Pur Ni-NTA resin (Thermo Fisher Scientific) followed by size-exclusion chromatography ...
-
bioRxiv - Cancer Biology 2020Quote: ... HA-tagged RON was cloned into the expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) by fusion PCR ...
-
bioRxiv - Microbiology 2020Quote: ... 1U Platinum High Fidelity (Hi-Fi) proof reading Taq polymerase (Invitrogen, Carlsbad, CA, USA). Two colonies of each G ...
-
bioRxiv - Microbiology 2020Quote: ... using the primers shown in Table II and cloned into pcDNA-V5/His (Invitrogen). pCAGGS-EboGP-V5 (strain Zaire 1976 Mayinga ...
-
bioRxiv - Immunology 2020Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher). To eliminate nonspecific binding proteins ...
-
bioRxiv - Molecular Biology 2020Quote: ... His-tagged hAgo2 was purified from Sf9 cells using a baculovirus system (Thermo Fisher). Cells were lysed in Lysis Buffer (300mM NaCl ...
-
bioRxiv - Microbiology 2022Quote: Pull-down assays were carried out using Dynabeads His-tag Isolation and Pulldown (Invitrogen). The 6His-tagged soluble pilin domains were used as bait ...
-
bioRxiv - Biochemistry 2022Quote: ... and cultured in house) were grown to 70% confluency in DMEM-Hi glucose (Gibco) supplemented with 10% heat-inactivated foetal bovine serum (Sigma) ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons were digested and ligated into pcDNA3.1 myc-His B expression vectors (Invitrogen), with a 3xFlag epitope tag also fused to the N-terminus of Ptchd1 ...
-
bioRxiv - Biochemistry 2022Quote: ... His-tagged protein was labeled with Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) per manufacturer’s recommendations ...
-
bioRxiv - Biochemistry 2022Quote: ... redundant anti-His antibody was washed away and 15 μL chemiluminescence substrate (Thermo Scientific) was added onto each well to generate chemiluminescence signal ...
-
bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI FBS, Gibco™ Cat. #10438026) and 1% antibiotic/antimycotic (Gibco™) ...
-
bioRxiv - Plant Biology 2022Quote: ... and purified by Dynabeads™ His-Tag Isolation and Pulldown (ThermoFisher, Catalog number 10103D). DA1 swapped protein ubiquitylation reactions used E1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... incubated for 30 minutes on ice with His-Pur Ni-NTA resin (Thermo Scientific), packed to a height of approximately 5 cm in polypropylene columns ...
-
bioRxiv - Microbiology 2024Quote: ... Supernatant was then processed through a His-tag cobalt resin (ThermoFisher, Waltham, MA, USA) following manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... and His-p115 were affinity purified using Ni-NTA HisPur beads (Thermo Fisher Scientific) from crude E ...
-
bioRxiv - Microbiology 2023Quote: ... A standard curve of 10-fold dilutions of plasmid pAc5.1-V5-His-A (Invitrogen) containing the DCV ORF-2 sequence was used to convert Ct values to genome copy numbers.
-
bioRxiv - Cell Biology 2024Quote: ... The His-tagged dAsapPZA protein was expressed and purified following manufacturer guidelines (Invitrogen, USA).
-
bioRxiv - Evolutionary Biology 2022Quote: ... and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (Thermo Fisher Scientific #MA1-21315) diluted in 5% milk in PBS-T (1:500) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Junction PCR was performed with Extensor Hi-Fidelity PCR Master Mix (Thermo Fisher Scientific) in a 20 μL reaction using 500 nM of target-specific primers and the following cycling conditions ...
-
bioRxiv - Immunology 2023Quote: ... M4 cells are maintained in DMEM/F12 media with 5% HI HS (Gibco #26050088).
-
bioRxiv - Neuroscience 2023Quote: ... and subcloned into the pcDNA™3.1/myc-His(−)A vector (Thermo Fisher Scientific). We accidentally obtained the pcDNA™3.1/myc-His(−)A-(G4C2)9 vector at this step ...
-
bioRxiv - Bioengineering 2023Quote: ... were first coated with 100 ng of mouse anti-His antibody (Invitrogen, MA1-21315) and blocked ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... We then used the Dynabeads His-Tag Isolation and Pull- down kit (ThermoFisher 10103D) to isolate his-tagged Fcy1 following manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... HI samples were incubated with 1 mL of IgG Fc Capture Select (Thermo Fisher) resin at 4C for 20 to 48 h to bind IgG ...
-
bioRxiv - Cancer Biology 2024Quote: ... The sample was then heated with Novex Hi-Density TBE sample buffer (Invitrogen LC6678) at 95°C for 5 min ...
-
bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat inactivated fetal bovine serum (HI-FBS) (10082147, Thermo Fisher Scientific) and 1% penicillin/streptomycin 1000 U/mL (Pen/Strep ...
-
bioRxiv - Developmental Biology 2024Quote: ... wild type Cic and mutant variants were subcloned into pMT-V5-His vector (Invitrogen).
-
bioRxiv - Microbiology 2024Quote: ... the His-tagged protein was purified using the ProBond Purification System (Thermo Fisher Scientific) and the eluted protein was dialyzed against PBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
-
bioRxiv - Cancer Biology 2024Quote: Isolated human PBMCs (ATCC PCS-800-011) were activated with human monoclonal antibodies against human CD3 (OKT3, Life Technologies, 16-0037) coated onto a flask and 2 ug/ml CD28 (CD28.2 ...
-
bioRxiv - Cell Biology 2020Quote: ... A Lsm12-KO cell line of HEK293 cells was generated with Synthego’s chemically modified sgRNA 5’-CCAGAAUGUCCCUCUUCCAG-3’ and GeneArt Platinium Cas9 nuclease (ThermoFisher Scientific) that were transfected together into cells using Lipofectamine CRISPRMAX Cas9 transfection reagent (ThermoFisher Scientific).
-
bioRxiv - Cancer Biology 2021Quote: ... After 24 hours the media was removed from the HEK293 CLK (NL-CLK1, CLK2-NL or CLK4-NL) transfected cells and replaced with 85 μL OPTI-MEM media (Gibco). NanoBRET Tracer 5 (Promega ...
-
bioRxiv - Molecular Biology 2020Quote: ... The wild-type complemented HEK293 RAD51 S14A mutant was generated by co-transfecting pcDNA5/FRT encoding wild-type RAD51 and pcDNA-DEST26 (Invitrogen), followed by single cell sorting (FACS ...
-
bioRxiv - Molecular Biology 2021Quote: HEK293 cells were purchased from ATCC and cultivated in DMEM medium supplemented with 10% fetal calf serum (Thermo Fisher Scientific). Cells were maintained at 37°C ...