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Citations for Thermo Fisher :
1001 - 1050 of 6996 citations for sCD4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK293) cells obtained from ATCC (Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293S GnTI− cells (ATCC CRL-3022) were grown in suspension at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Biophysics 2024Quote: ... the HEK293 cells were incubated with 1 μM Alexa Fluor-647 conjugated α-bungarotoxin (BTX-647; Life Technologies) at 4°C for 1 hr ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: The HEK293 Flp-In T-REx cell line was obtained from Life Technologies (Invitrogen catalogue number R780-07). HEK293 and HCT-116 cells (isolated from the colon of an adult male ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting plasmid was transfected into HEK293 cells at 70% confluency using Genejuice (Fisher Scientific 70-967-3) at a 1:3 ratio (DNA ...
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bioRxiv - Genomics 2024Quote: HEK293 were also cultured in Dulbecco’s Modified Eagle Medium 1X (41966-029) with 10% FBS (GIBCO, 10270-106) and 1% PIS in a 10 cm dish and maintained in an incubator at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... and VIC labelled human β actin endogenous control probe (Human - 4326315E) or RNA28S5 (Human - Hs03654441_s1) (Thermo Fisher Scientific) so that amplified mRNA can be normalised to β actin or RNA28S5 ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant fibroblast growth factor (human FGF2) (10 ng/ml, Invitrogen) was added to Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used for human samples were anti-human CD31 (#17031942, Invitrogen), anti-human CD45 (#17945942 ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% (V/V) fetal bovine serum (HI FBS heat inactivated, Gibco) and GlutaMAX-I (Gibco)39 ...
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bioRxiv - Microbiology 2019Quote: ... 1x Pen/Strep/Glu and 10% ultralow IgG HI-FBS (Thermo Fisher Scientific), and pelleted at 350 xg for 5 min ...
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bioRxiv - Immunology 2022Quote: ... The plate was coated with 2 µg/mL mouse anti-His antibody (Invitrogen cat ...
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bioRxiv - Immunology 2021Quote: ... HI sera were tested using PrioCHECK PRRSV Antibody ELISA Kit (Thermo Fisher Scientific) for detection of PRRSV N protein-specific antibodies ...
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bioRxiv - Plant Biology 2019Quote: ... and anti-His tag (Alexa Fluor 555 conjugated, Thermo Fisher MA1-21315-A555) for 3 hours at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... It was first amplified and inserted into pMT/V5-His B (ThermoFisher Scientific), using primers that introduced EcoRI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2020Quote: ... Gβ1 and His-Gγ2 (C68S) were synthesised and cloned into pFastBac Dual (Invitrogen). For mammalian expression ...
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bioRxiv - Biochemistry 2020Quote: N-terminally His-tagged P-Rex2 was synthesised and cloned into pFastBac1 (Invitrogen). His-tagged PTEN ...
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bioRxiv - Molecular Biology 2019Quote: ... Results were detected using Western Blotting using anti-His primary antibody (ThermoFisher, His.H8).
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bioRxiv - Biophysics 2019Quote: ... dye free liposome was prepared with TexasRed conjugated anti-His tag Antibody (ThermoFisher) by mixing lipids with antibody containing buffer ...
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bioRxiv - Microbiology 2021Quote: ... and subcloned into expression vector pBAD/myc-His A (Invitrogen, Carlsbad, CA, USA) that produces a fusion six-histidine tag at the end ...
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bioRxiv - Bioengineering 2021Quote: ... Blocking buffer was refreshed and mouse anti-His antibody (Thermofisher, Cat#MA1-21315) was added at a dilution factor of 1:2000 in blocking buffer ...
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bioRxiv - Biophysics 2021Quote: ... We used a rabbit anti-6X-His antibody (Invitrogen™, Waltham, MA, USA) to detect histidine-tagged proteins or mouse 1A9 antibody (GeneTex ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing was performed with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific) using Ion Proton instrument ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10 % V/V heat inactivated Fetal Calf Serum (Hi FCS, Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS- HI; Gibco) at 37 °C in a humidified 5% CO2 incubator ...
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bioRxiv - Cell Biology 2021Quote: The RNAP2 Ser2ph-mintbody sequence was inserted into pTrc-His (Thermo Fisher Scientific) to harbor the His-tag at the N-terminus ...
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bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using the Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher). Sequence reads were mapped to the hg19_AmpliSeq_Transcriptome_ERCC_v1.fasta reference ...
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bioRxiv - Microbiology 2020Quote: ... and His-PbChiB4 were purified with Ni-NTA agarose (Thermo Fisher Scientific, CAD) through native conditions following the manufacturer‘s recommendations ...
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bioRxiv - Biochemistry 2020Quote: ... Anti-His (C-term)-HRP (#46-0707, 1:2000 for dot blot, Invitrogen); Aβ (6E10 ...
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bioRxiv - Microbiology 2021Quote: ... PCR-mediated mutagenesis was performed using Phusion Hi Fidelity polymerase (Thermo Fisher Scientific) with the following thermocycler settings ...
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A novel immunopeptidomic-based pipeline for the generation of personalized oncolytic cancer vaccinesbioRxiv - Cancer Biology 2021Quote: ... 10% heat inactivated fetal bovine serum (HI-FBS, GIBCO, Invitrogen, Carlsbad, CA, USA) and 1% Penicillin-Streptomycin (10,000 U/mL ...
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A novel immunopeptidomic-based pipeline for the generation of personalized oncolytic cancer vaccinesbioRxiv - Cancer Biology 2021Quote: ... 10% heat inactivated fetal bovine serum (HI-FBS, GIBCO, Invitrogen, Carlsbad, CA, USA) and 1% Penicillin-Streptomycin (10,000 U/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Samples (2 µL) were suspended in 10 µL Hi-Di formamide (Thermo Fisher) and 0.5 µL MapMarker ROX 1000 (Eurogentec ...
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bioRxiv - Genetics 2020Quote: ... reactions were diluted with Novex Hi-Density TBE Sample Buffer (Thermo Fisher LC6678), and run on precast TBE gels ...
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bioRxiv - Molecular Biology 2022Quote: ... PfP2 sample was purified after binding with Hi-Bind Ni-NTA beads (Invitrogen) for 4h followed by elution of the sample with 20mM Tris-HCl (pH 7.4) ...
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bioRxiv - Developmental Biology 2020Quote: ... each experiment sample was separated on a Hi-pH column (Thermo Fisher Scientific) according to the vendor’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... The His-tagged proteins were purified on Ni-NTA resin (Thermo Scientific #88222) and washed into a buffer containing 50 mM Tris (pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: MBP-His-TEV-Epe1 was cloned into a pFastBac vector (Thermo Fisher Scientific) and used for Bacmid generation ...
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bioRxiv - Microbiology 2021Quote: ... The products were cloned into the pcDNA3.1D/V5-His’s Topo expression vector (Invitrogen) and NL4.3 ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His Tag Antibody (clone HIS.H8) (1:1000, # MA1-21315) was from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and into pVL847 (His-MBP-tag overexpression vector) by Gateway cloning method (Invitrogen). ΔnahA mutant of Bacillus subtilis was constructed by CRISPR/Cas9 editing method (Ref) ...
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bioRxiv - Molecular Biology 2021Quote: Western blot analysis was performed using an anti-His antibody (Invitrogen, LOT 1902132) to confirm the identity of splicing and cleavage products ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Thermo Fisher Scientific) and 1% Pen-Strep (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... plates were coated with 1 μg/mL 6x-His tag polyclonal antibody (Invitrogen) in 1x PBS for 6 hours at ambient temperature and blocked overnight with 0.5% NCS/5% Goat Serum/5% Milk/0.2% PBS-T ...
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bioRxiv - Immunology 2021Quote: ... or left unstimulated in 10% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) containing Brefeldin A (eBioscience cat ...
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bioRxiv - Microbiology 2021Quote: ... gingivalis were stained with 15 mg/l hexidium iodide (HI; Thermo Fisher Scientific) and 4 mg/l 5-(and-6)-carboxyfluorescein and succinimidyl ester (FITC ...
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bioRxiv - Cell Biology 2021Quote: ... before being incubated with the primary antibody (6x-His Tag Monoclonal Antibody, Invitrogen) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... and His-tagged mCherry (strain He/80/FR) were synthesized by Thermo Fisher Scientific (Waltham ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...