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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... in PBS and incubated for 2-3 hr in secondary antibodies (conjugated to Alexa-488, Alexa-568, and Alexa 647, Life Technologies,1:500) at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Rehydrated embryos were blocked with 10% BSA (in PBST) for 2-3 hours and incubated with anti-GFP antibody (1:1000; ThermoFisher Scientific, A-11120) overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... We incubated overnight with the anti-GFP primary antibody at 4 degrees (1:1000, A-6455 Invitrogen, 0.1M PBS 0.3% tx100, 3% NGS). After abundant PBS washes ...
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bioRxiv - Neuroscience 2021Quote: ... They were then washed 3 times in PBS and incubated with a secondary anti-rabbit GFP antibody dissolved in PBST (1:1000, AF546, ThermoFisher, Waltham, MA, USA) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... gels were incubated for 3 hr with a secondary antibody solution containing goat anti-rabbit Alexa Fluor 488 IgG (1:400, Thermo Fisher Scientific, A11008) and goat anti-mouse Alexa Fluor 568 IgG H +L (1:400 ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then washed 3 times in PBS and incubated with appropriate fluorochrome secondary antibody (AlexaFluor® 488, AlexaFluor® 568, 1:400, Life Technologies), or biotinylated secondary antibody (Vector Labs ...
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bioRxiv - Biophysics 2021Quote: ... cells were incubated in blocking buffer with 8 ng/μL of custom labeled secondary antibodies or of commercial IgG-gam-F(ab’)2-Alexa Fluor 647 (DOL ~ 3) (Thermo Fisher, A-21237) for 45 min ...
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bioRxiv - Cell Biology 2021Quote: ... 3 x 1 h) and incubated with the secondary antibody (Alexa-labelled goat anti-mouse 555 plus, A32727, Thermo Fisher Scientific, 1:1000), an anti-GFP antibody conjugated with Alexa 488 (A-21311 ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were washed 3× for 10 min with PBS and incubated with the anti-rabbit secondary antibody (1:1000; Alexa Invitrogen, Walthan, United States), diluted in blocking solution ...
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bioRxiv - Biophysics 2022Quote: ... Cells were rinsed 3 times by 1X PBS and labeled with 500 μL of 1:100 anti-alpha Tubulin primary antibody (Thermo Fisher Scientific, 322500), 1:200 anti-Tomm20 primary antibody (Abcam ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed twice in PBS with 0.10% Tween-20 for 3 minutes each and treated with secondary antibodies conjugated with Alexa Fluor 488 (Invitrogen A-11039, 1:200) and/or Alexa Fluor 568 (Invitrogen A-11004 ...
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Synaptic Zn2+ potentiates the effects of cocaine on striatal dopamine neurotransmission and behaviorbioRxiv - Neuroscience 2021Quote: ... Tissue was washed with washing buffer 3 × 10 min at RT then incubated in secondary antibody (Alexa 488-Rabbit #A1134, Thermo Fisher (1:400), Topro (1:1200) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in blocking buffer at room temperature 3 x for 15 minutes each after which secondary antibodies were prepared using goat anti-rabbit (Invitrogen A10520; 1:500) and goat anti-rat (Invitrogen A11006 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed with Bond Wash solution 3× 3 minutes then incubated with a goat anti-rabbit IgG (H+L) AlexaFluor 633-conjugated secondary antibody (A-21070, ThermoFisher Scientific, Waltham, MA) 1:500 in PBS containing 3% goat serum ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed with 1X PBS 3 times prior to incubation with fluorochrome-conjugated secondary antibody (goat anti-rabbit Alexa Fluor 647, ThermoFisher, Cat. #A-21245) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with a secondary goat anti-rabbit IgG antibody (10–3) coupled to Alexa Fluor Plus 594 (Molecular Probes Life Tech.) for 60 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 7.4] for 3 hours at room temperature (RT) followed by incubating with a rabbit anti-EGFP antibody (ThermoFisher G10362, 1:1000 dilution) at 4 °C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... HLA-DR Antibody (Monoclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:50 Clone: LN-3, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), TMEM119 (Rabbit Anti-Human ...
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bioRxiv - Neuroscience 2022Quote: ... samples were washed three times for 3 minutes and then incubated 1 hour at RT with secondary antibody (1:500, #A21202, #A21449, #A10042, #A32731, Life Technologies, Carlsbad, California) and Hoechst 33342 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Dishes were washed with PBS 5 times for 3 min each before applying donkey anti-mouse Alexa Fluor 568 secondary antibodies (A10037, Invitrogen; 1:2000 diluted) and Alexa Fluor 647 phalloidin (A22287 ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then washed 3 times with PTw before incubating with the secondary antibody (goat anti-rabbit alexa488, 1:500, Thermo Fisher A-11008) and DNA stain (Hoechst34580 1:1000 Sigma Aldrich 63493) ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed 3 times with 200 µL PBS for 4 min before secondary antibodies and 1 µg/mL Hoechst (Thermo Fisher Scientific #33342) were added in 35 µL per well and incubated for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed 3-times with 1× PBS and stained with Alexa Fluor 568 goat anti-mouse IgG1 secondary antibody (Life Technologies, catalog # A21124) at room temperature for one hour ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed 3 times and then incubated with the goat anti-rabbit secondary antibodies Alexa Fluor® 488 (1:1,000, Thermo Fisher Scientific, USA) for ESCRT-III ...
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bioRxiv - Immunology 2022Quote: PGT145 and VRC26-family antibodies were captured with Protein A columns (Cytiva, 11003493) and gently eluted with 3 M MgCl2 solution (Thermo Fisher Scientific, 21027). Elutions were then buffer exchanged to HEPES buffer (10 mM HEPES pH 8.0 and 75 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were rinsed (3 times) with 2 ml incubation buffer and resuspended with anti-mouse Alexa Fluor 647 secondary antibody (1:2000, Invitrogen, Carlsbad, CA, USA) for 60 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... Tissues were incubated with primary antibodies (mouse anti-HuC/D IgG clone 16A11 at 1:200 [ThermoFisher], rabbit anti-tubulin β-3 (TuJ1) polyclonal IgG at 1:500 [Biolegend] ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with cold 1× PBS-T for 3 times each for 10 min then incubated for 2 hours at RT with secondary antibodies (AF546-Ms: Invitrogen A11003, 1:500) diluted in 1× PBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... retinas were incubated for 3 hours at room temperature with Alexa Fluor-conjugated secondary antibodies (1:1,000; Thermo Fisher Scientific or Jackson ImmunoResearch). Whole retinas were flattened with photoreceptor side down onto nitrocellulose filters ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated at room-temperature for 3-4 hours with secondary antibody mixture containing Alexa Fluor 568 anti-mouse (1:500; Thermo Fisher Scientific, A11031) and Alexa Fluor 647 anti-chicken (1:500 ...
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bioRxiv - Molecular Biology 2024Quote: ... washed 3 times with PBS and incubated with the appropriate anti-species secondary antibodies coupled with Alexa fluorochromes (Thermo Fisher Scientific, 1:2000) and DAPI (Sigma ...
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bioRxiv - Immunology 2024Quote: ... The membranes were washed 3 times with TBS for 5 minutes each and then incubated with a secondary antibody (Thermo Fisher Scientific, US). An enhanced chemiluminescence (ECL ...
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bioRxiv - Neuroscience 2024Quote: ... in 1X PBS-0.3% Triton X-100 (0.3% PBST) before 2-3 day incubation with primary antibodies at 4C for PCP4 (1:250, Invitrogen Cat# PA5-52209, RRID:AB_2645298) or NECAB2 (1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated with secondary antibody (AlexaFluor 546 Donkey anti-mouse for PV, goat anti-rabbit for GluR2/3 or NPY, both 1:400, Thermo Fisher Scientific Inc.) in secondary antibody solution (1% normal donkey serum for PV or 1% normal goat serum for GluR2/3 or NPY ...
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bioRxiv - Cancer Biology 2024Quote: ... The lysate was precleared with IgG for 3-4h at 4 degrees before the overnight incubation with human TROP2 antibody (Invitrogen, 14-6024-82) or mouse TROP2 (R&D ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed in PBS (3 x 10 minutes) and then incubated with secondary antibodies consisting of Alexa 488 goat anti-mouse (Invitrogen #A11029, 1:1000) and Alexa 555 goat anti-rabbit (Invitrogen #A21429 ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were then washed in PBS 3 times and incubated with appropriate fluorescence–conjugated secondary antibodies (Jackson ImmunoResearch Laboratories or Thermo Scientific Invitrogen) at 1:1000 dilution and DAPI (1:1000 dilution ...
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bioRxiv - Plant Biology 2020Quote: ... Total phosphorus concentrations were analyzed by sector-field high-resolution inductively coupled plasma mass spectrometry (HR-ICP-MS; ELEMENT 2, Thermo Fisher Scientific, Germany). Element standards were prepared from certified reference standards from CPI-International (USA).
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bioRxiv - Evolutionary Biology 2020Quote: ... The Peltier elements were mounted with thermal paste to aluminum blocks through which distilled water was pumped from a circulating chiller (ThermoFisher TF2500 Recirculating Chiller). Design files for the behavioral arena and Arduino controller are available in the data and analysis code repositories ...
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bioRxiv - Plant Biology 2020Quote: ... LHCSR3.1 promoter was then placed upstream of the secretion signal and reporter gene by digesting vector and 1.5-kb element with BamHI and XbaI FastDigest® restriction endonucleases (Thermo Scientific, Waltham, Massachusetts). Construct ProLHCSR3.1:cCAgLuc was then transformed into cc124 and hit1 by electroporation as previously described (Jaeger et al. ...
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bioRxiv - Microbiology 2022Quote: ... Analyses were done using the inductively coupled plasma sector-field mass spectrometer (ICP sfMS) Element XR-2 with jet interface (Thermo Fisher Scientific, Germany) following a described protocol [50] ...
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bioRxiv - Physiology 2022Quote: ... Major/minor element analyses for digested samples were carried out at Princeton University using a quadrupole inductively coupled plasma mass spectrometer (Thermo Scientific iCap Q). Concentrations and elemental ratios were determined using externally calibrated standards and average uncertainties (element/element ...
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bioRxiv - Genetics 2024Quote: All LG4 and promoter elements used in electrophoretic mobility shift assays were cloned into TOPO TA pCR2.1 (Invitrogen cat no. 45-064-1) in both the sense and antisense orientations and verified by sequencing ...
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bioRxiv - Plant Biology 2023Quote: ... Detection was achieved with a customized sector field ICP-MS (Element XR-2 with jet interface and desolvating injection, Thermo Scientific, Bremen Germany) and a diode array detector coupled to a metal-free HPLC system (Azury system ...
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bioRxiv - Immunology 2022Quote: Murine 3XFLAG-tagged Spi-C cDNA (35) was PCR amplified and ligated into the pRetro-Tre3G (Tet-regulatable element third generation) plasmid (Thermo-Fisher Scientific, Waltham MA). MIG-Tet3G was previously described (24) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and mass concentrations was performed using a scanning mobility particle sizer (SMPS) and a tapered element oscillating microbalance (TEOM 1400a, Thermo Fisher Scientific, USA). The SMPS was operated at an aerosol flow of 0.3 L min−1 with an X-ray neutralizer (TSI ...
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bioRxiv - Genetics 2021Quote: ... The CRL-1730 adherent human vascular endothelial cell line derived from umbilical cord vein (ATCC, (Shioda et al., 2018)) was cultured in F-12K medium (Gibco/ThermoFisher Scientific) supplemented with 10% heat inactivated foetal bovine serum (Gibco/ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: Bone marrow derived dendritic cells (BM-DCs) cells from either wt or ifitm3-/- mice were grown in SILAC RPMI media (Gibco, Thermo-Fisher) either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Viral supernatants were derived by transient co-transfection of 293T (6×106 in 100 mm3 petri-plates) cells using lipofectamine 2000 (Thermo Fisher Scientific). A total of 19.5 μg of plasmid in the proportion of 12 μg of lentiviral plasmid carrying human/murine FAP-α cDNA ...
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bioRxiv - Neuroscience 2020Quote: ... HCV-29 human derived urothelial cells were obtained as a gift from Paul Brindley and grown in MEM (Thermofisher Scientific, Waltham, MA) with 10% fetal bovine serum (Sigma-Aldrich ...