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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for PD 1 Human HEK293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: HEK293 (XX female) cells and HepG2 cells were cultured in high glucose Dulbecco’s modified eagle medium (DMEM) (41965, Thermo Fisher) and supplemented with 10% fetal bovine serum (10270 ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... slides were washed thrice for 5 min in PBS before incubation with Alexa 594 goat-anti rabbit secondary antibody at 1:1,000 and Alexa Fluor 488 goat anti-human IgG(H+L) at 1:500 (both Invitrogen; in PBS) for 2 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... EpiLCs were induced by plating 1.5 x 106 ESCs on a 10 cm dish coated with human plasma fibronectin (16.7 mg/ml) in the 1:1 mix of DMEM/F12 (Gibco, 11302-33) and Neurobasal medium containing N2 supplements ...
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bioRxiv - Evolutionary Biology 2020Quote: ... a formulation optimal for human fibroblasts [(Dickson et al., 2000): 1:1 (vol/vol) medium M199 and medium M106 (ThermoFisher Scientific) + 15% BCS + 10 ng/ml EGF + 0.4 ug/ml hydrocortisone + 1% Penicillin-Streptomycin] ...
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bioRxiv - Biophysics 2024Quote: ... primary stained with goat anti-Syndecan-1 antibody and mouse anti-human choriogonadotropin (β-hCG) antibody (#14-6508-82, 1:500 dilution in the buffer solution, ThermoFisher), and secondary stained as previously described ...
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bioRxiv - Microbiology 2023Quote: ... and was then incubated (1:1000 dilution) for 1 hour with a mouse anti-human E-Cad ectodomain antibody (HECD-131700, 1:1000 dilution; Life Technologies). After two washes ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used in this study were all anti-human: STING (1:1000, CST, #13647 or 1:500, Thermo Fisher, MA526030), UBIQUITIN (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
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bioRxiv - Bioengineering 2023Quote: ... primary T cells were activated and stimulated with a 1:1 ratio of anti-human CD3/CD28 magnetic Dynabeads (Thermo Fisher) to cells in X-vivo 15 medium supplemented with 5% human AB serum (Heat inactivated) ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were incubated with a 1:200 dilution of human cTnT primary mouse antibody (ThermoFisher) overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... cells were stained with a PE-conjugated anti-human IgG1(Fc) secondary antibody (1:50) (ThermoFisher) for additional 15min ...
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bioRxiv - Neuroscience 2019Quote: ... or 10 μM of freshly prepared human synthetic amyloid-β peptide 1-42 (Aβ42, Life technologies) for 24 h as described (Kiyota et al. ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human TNF-α a well established trigger of HIV-1 reactivation was purchased from Gibco. The PKC activator Bryostatin and Sodium Butyrate (NaBu ...
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bioRxiv - Microbiology 2020Quote: The human acute leukemia monocyte cell line (THP-1) was cultivated in RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated FBS (Thermo Scientific Hyclone ...
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bioRxiv - Biochemistry 2022Quote: ... The ORF of human MYBL2/B-Myb isoform 1 (NM_002466.4) was cloned into pcDNA3.1+ (ThermoFisher Scientific) and fused with an N-terminal Flag tag ...
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bioRxiv - Molecular Biology 2022Quote: ... Mouse-anti-human H3K9/14/18/23/27ac (Thermo Fisher Scientific, Carlsbad, CA, USA; 1:500) was used to detect this histone modification in HPdLF and goat-anti-Mouse-Cy5 (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2021Quote: ... Alexa488- or Rhodamine-conjugated goat anti-human secondary antibody was added (1/500 dilution) (Thermo Fisher), and incubated for 2~3 hr at room temperature in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... Immortalized human fibroblasts were cultured in DMEM (sigma) supplemented with 1% of penicillin and streptomycin (Gibco) and 10% fetal bovine serum (Corning ...
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bioRxiv - Immunology 2020Quote: ... plates were incubated with goat polyclonal anti-human IgG HRP-conjugate (1/1000; Thermo Fisher Scientific) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... plates were incubated with a goat anti-human IgG HRP-conjugate (1/1000; Thermo Fisher Scientific). For IgG subclasses and IgM detection ...
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bioRxiv - Immunology 2021Quote: Human THP-1 cells (ATCC® TIB-202™) were cultured in RPMI media (22400089, ThermoFisher) supplemented with 1% (v/v ...
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bioRxiv - Immunology 2021Quote: ... diluted 1:3000 in blocking buffer or HRP-labelled goat anti-human IgM (Thermo Fisher Scientific) diluted 1:4000 at 37°C for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... and 1:5,000 diluted goat anti-human-IgG Fc horseradish peroxidase (HRP) conjugated secondary antibodies (Invitrogen) were added ...
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bioRxiv - Developmental Biology 2022Quote: ... CD31-APC with human specificity for HUVEC experiments (1:500, Thermo Fisher Scientific, 17-0319-42) together with its IgG1 kappa APC-Isotype control (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ng/mL human basic FGF2 (Miltenyi Biotech, 130-093-840) and kanamycin (Gibco, 15160-054). Culture medium was exchanged every 2 days ...
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bioRxiv - Bioengineering 2023Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-human IgM and IgA (Invitrogen, A18835 and A18781, 1:5,000), rabbit anti-human IgG antibody (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: DNA encoding the ECR of human ADGRB2/BAI2 (aa 1-921) was synthesized by Thermo Fisher GeneArt ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-Human IgG (H+L) Alexa Fluor 488 conjugated (Invitrogen, A-11013, dilution 1:500); Donkey Anti-Mouse IgG H&L Alexa Fluor 647 conjugated (Abcam ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-Human IgG (H+L) Alexa Fluor 488 conjugated (Invitrogen, A-11013, dilution 1:500). Data acquisition was performed with FACSVerse™ (BD ...
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bioRxiv - Neuroscience 2024Quote: ... the mean fluorescence intensity (MFI) of Alexa488-coupled goat anti-human IgG (1:500; Life Technologies) was evaluated ...
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bioRxiv - Cancer Biology 2023Quote: 1 × 105 OCSC1-F2 human OC cells were resuspended in serum-free Opti-MEM (Thermo Scientific) and seeded on trans-well inserts with a polyethylene terephthalate membrane pore size of 8.0 μm (Cat ...
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bioRxiv - Neuroscience 2023Quote: ... A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA, 88881, Life Technologies) enabled in vitro translation of these constructs ...
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bioRxiv - Immunology 2024Quote: ... plates were incubated with a goat anti-human IgG HRP-conjugate (1/1000; Thermo Fisher Scientific). For IgG subclasses and IgM detection ...
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bioRxiv - Cell Biology 2024Quote: ... Samples with 1×106 isolated human platelets were prepared in binding buffer (Invitrogen, Carlsbad, CA, USA). Hemin and CPR-XL were incubated at the indicated concentrations for 1h at room temperature.
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bioRxiv - Neuroscience 2024Quote: Human primary Müller cells (P3) after siRNA treatments were incubated with JC-1 dye (#T3168, Invitrogen) at a concentration of 1.0 μg/mL in DMEM at 37 °C for 30 minutes to measure the mitochondrial membrane potential ...
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bioRxiv - Cell Biology 2024Quote: ... PC3 cells were transfected with cDNA encoding human talin-1 (NM_006289) using Lipofectamine 3000 reagent (Invitrogen) according to the manufacturer’s recommendation ...
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bioRxiv - Immunology 2024Quote: ... plates were incubated with goat polyclonal anti-human IgG HRP-conjugate (1/1000; Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: The raw data generated for each sample were analyzed using the Proteome Discoverer (PD) 1.4 software (Thermo Scientific, San Jose, CA). Protein identification was done by mapping against a customized protein sequence database combined from UniProt Homo sapiens proteome ...
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bioRxiv - Biophysics 2021Quote: NCI-H1299 human non-small cell lung cancer cells with high expression of PD-L1 were purchased from the American Type Culture Collection (ATCC) and cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... PD-L1: Hs00204257_m1) for PCR amplification and detection on the HT Fast Real-Time PCR System (Applied Biosystems from Life Technologies). GAPDH was used as an endogenous normalization control ...
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bioRxiv - Cancer Biology 2020Quote: ... PD-L1: Hs00204257_m1) for PCR amplification and detection on the HT Fast Real-Time PCR System (Applied Biosystems from Life Technologies). GAPDH was used as an endogenous normalization control ...
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bioRxiv - Biochemistry 2022Quote: ... Excess dye was removed by desalting using either a PD-10 gravity column (Cytiva Life Sciences) or a Zeba desalting spin column (Thermo-Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: HK1 grown in 100 mm plates were transfected with 5 μg of RFP-LMP1 or GFP-PD-L1 plasmid using Lipofectamine 3000 transfection kit (Invitrogen, L3000015). Lipofectamine reagents were diluted in Opti-MEM medium (Gibco ...
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bioRxiv - Biochemistry 2020Quote: Raw files obtained from IGX-MS of GroEL were analyzed with the Proteome Discoverer (PD) software suite version 2.3 (Thermo Fisher Scientific) with the incorporated XLinkX node for analysis of cross-linked peptides ...
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bioRxiv - Cell Biology 2022Quote: Human dermal primary fibroblasts isolated from a PD patient (ND39528) and a healthy control (ND36091) were cultured in DMEM (11995-065, Thermo Fisher) supplemented with 10% fetal bovine serum (900-108 ...
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bioRxiv - Plant Biology 2022Quote: ... The .raw mass spectral files were searched using the SEQUEST algorithm of the Proteome Discoverer (PD) software version 2.1 (Thermo Fisher Scientific). Tolerances were set to 10 ppm and 0.8 Da to match precursor and fragment monoisotopic masses ...