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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for PD 1 Human HEK293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 1:500 dilution) (goat anti-human IgG Fc Alexa 488 conjugated, Thermo Fisher, Cat #: H10120) (goat anti-human IgG (H + L ...
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bioRxiv - Immunology 2022Quote: ... mouse-anti-mouse/human/rat monoclonal PP1a (1:1000 dilution, number MA5-17239, ThermoFisher Scientific), rabbit-anti-Y.pestis polyclonal YopM (1:1000 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... at a dilution 1:2000 and rabbit anti-OCN antibody (Reactivity: human, Invitrogen, PA5-96529) at a dilution 1:100 in 1% BSA in PBS-T for 1h00 at room temperature ...
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bioRxiv - Bioengineering 2024Quote: Primary human hepatocytes were transfected 1 day after thawing using Lipofectamine MessengerMAX Transfection Reagent (Invitrogen) using the following protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Human-Kinesin-1-EGFP-expressing Drosophila S2 cells were grown in Schneider’s medium (Life Technologies) supplemented with 10% heat inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... and Alexa Fluor 488 goat anti-human IgG (H+L) (Invitrogen, cat # A11013, 1:1000).
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bioRxiv - Genetics 2023Quote: Cultured human iPSC-derived neurons (differentiation protocol 1) were harvested in TRIzol reagent (Invitrogen #15596026). Following TRIzol/chloroform extraction ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed in PBS and incubated with anti-human Alexa-647 (1:500, ThermoFisher), and Hoechst 33342 (1:5000 ...
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bioRxiv - Genetics 2022Quote: ... and stained with a monoclonal mouse anti-human HbF antibody (clone HBF-1, ThermoFisher scientific). Cells were analyzed by flow cytometry using a BD LSRFortessa cell analyzer (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant human Epithelial Growth Factor 5 µg.L-1 (Ref AF-100-15; PEPROTECH, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were pretreated for 1 h with 10 ng/mL human recombinant FGF-2 (Gibco) or vehicle (sterile water) ...
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bioRxiv - Cell Biology 2023Quote: Human primary monocytes and THP-1 cell lines (ATCC) were cultured in RPMI 1640 (Gibco) supplemented with 10% FBS (Sigma ...
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bioRxiv - Immunology 2023Quote: ... in presence of Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, from Thermofisher, USA) and IL-2 (50 U/mL ...
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bioRxiv - Cell Biology 2024Quote: ... and Alexa Fluor 555 goat anti-human IgG (H+L) (A21433) (1:500, Thermo Fisher).
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated with primary antibodies against human αS (1:1000; 32-8100, mouse; Invitrogen), α-phospho-Serine129 (pS129 ...
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bioRxiv - Molecular Biology 2020Quote: ... Human transferrin-568 (ThermoFisher) was added at 10μg mL−1 concentration in serum free media and incubated at 37°C to allow for internalization ...
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bioRxiv - Immunology 2021Quote: ... then anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 50 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: Probes Hs00171064_m1 (human, ThermoFisher), Mm00440280_g1 (mouse ...
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bioRxiv - Bioengineering 2022Quote: ... Primary human hepatocytes (Gibco) were seeded in the top channel at a density of 3.5 x 106 cells/mL using complete hepatocyte seeding media ...
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bioRxiv - Immunology 2020Quote: ... or anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 25 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... or human IgG (Invitrogen) as control ...
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bioRxiv - Bioengineering 2022Quote: ... and human fibronectin (Gibco) at 50 μg/mL in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... human (ThermoFisher Scientific, 902927). Analysis was performed with Transcriptome Analysis Console 4.0 software (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... then anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 50 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... CD36 (Human tissue Thermofisher: PA1-16813 1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... human plasma (ThermoFisher, #33016015); Laminin 111 ...
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bioRxiv - Immunology 2023Quote: ... A Human ProcartaPlexTM (Invitrogen) immunoassay was additionally used to detect 45 human cytokines ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human-HRP (Invitrogen) was diluted 1:5000 in 1% BSA/PBS ...
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bioRxiv - Cell Biology 2024Quote: ... human recombinant insulin (ThermoFisher) was added at 100 nM for 30 minutes and Smoothened agonist (SAG ...
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bioRxiv - Cancer Biology 2024Quote: ... human CD3-PE (Invitrogen), and mouse CD45-erpCP Cy5.5 (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
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bioRxiv - Cancer Biology 2024Quote: Isolated human PBMCs (ATCC PCS-800-011) were activated with human monoclonal antibodies against human CD3 (OKT3, Life Technologies, 16-0037) coated onto a flask and 2 ug/ml CD28 (CD28.2 ...
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bioRxiv - Neuroscience 2020Quote: ... MS/MS database search was performed against in silico tryptic digest of mouse proteins UniProt FASTA database using SEQUEST HT within Proteome Discoverer 2.2 (PD 2.2, Thermo Fisher Scientific) with precursor mass tolerance of 10 ppm and fragment mass tolerance of 0.02 Da ...
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bioRxiv - Physiology 2019Quote: ... Peptides were identified from the resultant spectra using SEQUEST in the Proteome Discoverer 2.2 (PD 2.2) software package (Thermo Fisher Scientific) and custom-built databases ...
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bioRxiv - Immunology 2022Quote: ... Human peritoneal macrophages (hPMφ) were isolated from dialysate of PD patients as previously described[20] and cultured in RPMI 1640 medium (Gibco) with 10% FBS (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... Isotopically resolved masses in precursor (MS) and fragmentation (MS/MS) spectra were processed in Proteome Discoverer (PD) software (v2.4, Thermo Scientific). All MS/MS samples were analyzed using Mascot (Matrix Science ...
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bioRxiv - Cell Biology 2019Quote: All MS/MS spectra were searched by Sequest/Percolator under the software platform Proteome Discoverer (PD 1.4, Thermo Fisher Scientific) using a target-decoy strategy ...
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bioRxiv - Cell Biology 2021Quote: ... medium was exchanged to DMEM with 10% PDS and cells were transfected with 40 nM siRNA using lipofectamine 3000 (Invitrogen). On day six ...
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bioRxiv - Cancer Biology 2021Quote: ... 80% confluence MRC5 cells (PD 15-25) were transfected using 500ng of plasmid per well with Lipofectamine 3000 (ThermoFisher L3000001). For selection ...
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bioRxiv - Biophysics 2020Quote: Peptides were identified from the resultant MS/MS spectra by searching against a drosophila proteome database downloaded from UniProt using SEQUEST in the Proteome Discoverer 2.2 (PD 2.2) software package (Thermo Fisher Scientific). The potential loss of methionine from the N-terminus of each protein (−131.20 Da) ...
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bioRxiv - Neuroscience 2023Quote: ... Peptide search was performed with Sequest HT and TMT quantification was performed with the reporter ions node in Proteome Discoverer 2.5 (PD, Thermo Fisher). Data was searched against reviewed mouse sequences in Uniprot ...
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bioRxiv - Cancer Biology 2023Quote: PD-L1 expression on tumor cells: A549 cells incubated with stimulated T cells were also analyzed for PD-L1 expression (APC, Invitrogen) and viability with 7AAD staining.
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bioRxiv - Physiology 2024Quote: ... raw data files were searched against a composite target/decoy database using SEQUEST from Proteome Discoverer software (PD, v.2.2, ThermoFisher Scientific). The proteome reference UP000000589 database ...
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bioRxiv - Biophysics 2024Quote: ... or Danio rerio (downloaded from UniProt 2/2015) proteome database using SEQUEST in the Proteome Discoverer 2.2 (PD 2.2) software package (Thermo Fisher Scientific). The potential loss of methionine from the N-terminus of each protein (-131.20 Da) ...
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bioRxiv - Neuroscience 2024Quote: Isotopically resolved masses in the precursor (MS) and fragmentation (MS/MS) spectra were analyzed with Proteome Discoverer (PD) software (v2.4, Thermo Scientific). All data were searched by Mascot (2.6.23 ...
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bioRxiv - Cell Biology 2020Quote: ... A Lsm12-KO cell line of HEK293 cells was generated with Synthego’s chemically modified sgRNA 5’-CCAGAAUGUCCCUCUUCCAG-3’ and GeneArt Platinium Cas9 nuclease (ThermoFisher Scientific) that were transfected together into cells using Lipofectamine CRISPRMAX Cas9 transfection reagent (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... After 24 hours the media was removed from the HEK293 CLK (NL-CLK1, CLK2-NL or CLK4-NL) transfected cells and replaced with 85 μL OPTI-MEM media (Gibco). NanoBRET Tracer 5 (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... The wild-type complemented HEK293 RAD51 S14A mutant was generated by co-transfecting pcDNA5/FRT encoding wild-type RAD51 and pcDNA-DEST26 (Invitrogen), followed by single cell sorting (FACS ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells were purchased from ATCC and cultivated in DMEM medium supplemented with 10% fetal calf serum (Thermo Fisher Scientific). Cells were maintained at 37°C ...
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bioRxiv - Neuroscience 2020Quote: The HEK293 cells (CRL-1573, ATCC, USA) were kept in the Dulbecco’s Modified Eagle Medium (High Glucose)-DMEM (Invitrogen, USA) supplemented with 10% fetal bovine serum-FBS (Sigma-Aldrich ...