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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... containing 2’-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 50 μM; Thermo Fisher Scientific). To determine FA uptake ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Immunology 2023Quote: ... stained with Ghost-510 and 2-(N-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 60 µM) (Thermo Fisher Scientific) (30 min at 37 C) ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of 100 μM oligonucleotide bridge 2 and 2 units of T4 PNK (ThermoFisher) were added to 20 μl of the purified smDNA solution ...
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bioRxiv - Cancer Biology 2021Quote: ... PXDN activity was measured by adding 50 μL/well of 50 μM Amplex™ UltraRed Reagent (Thermo Fisher Scientific), 20 μM hydrogen peroxide and 50 mM bromide in 50 mM phosphate buffer pH 7.4 ...
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bioRxiv - Genomics 2020Quote: ... media were replaced with 100 μL full melanoma media without phenol-red containing 4μM Caspase-3/7 activity dye (CellEvent, ThermoFisher). Plates where imaged using a Celigo Imaging Cytometer (Nexcelom ...
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bioRxiv - Cancer Biology 2019Quote: ... Enzyme activity was stopped by addition of equal volume of 4% BSA in Earle’s Balanced Salt Solution (EBSS, Gibco). The cell suspension was filtered through a 70 μm cell strainer and centrifuged at 1200 rpm for 5 min and the pellet was re-suspended in 0.9 M sucrose in 0.5 x HBSS (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies were conjugated to horseradish peroxidase (HRP) and peroxidase activity was visualized using Chemiluminescent HRP substrate (Thermo Scientific).
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bioRxiv - Bioengineering 2022Quote: Metabolic activity of hMSC seeded scaffolds was determined using a non-destructive alamarBlue® assay (Invitrogen, Carlsbad, CA, USA) at days 3 ...
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bioRxiv - Developmental Biology 2022Quote: ... ETC complex activity assays were normalized to cell lysate protein content via Bicinchoninic Acid (BCA) assay (Pierce, ThermoFisher Scientific) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies were conjugated to horseradish peroxidase (HRP) and peroxidase activity was visualized using Chemiluminescent HRP substrate (Thermo Scientific). Western blot densitometry analysis was performed using ImageJ and are expressed relative to the first lane control and normalized to the indicated loading control ...
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bioRxiv - Microbiology 2019Quote: Stationary-phase reductase and electron transport chain activities were measured with Redox Sensor Green (RSG) dye (ThermoFisher, catalog# B34954) according to manufacturer’s instructors ...
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bioRxiv - Genomics 2022Quote: ... After incubation enzyme activity was inactivated by adding 50 μl of 20 mM EDTA/PBS (Lonza, pH 7.4 Gibco) shaking at 300 rpm 37 °C incubating for 5 min ...
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bioRxiv - Plant Biology 2023Quote: ... Epitope-tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... Myc-tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... mYFP tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Genomics 2023Quote: ... cells were assayed for their lysosomal hydrolase activity by incubating with 0.2 ug/mL Hoechst 33342 and either 200 uM PFB-FDGlu (for glucosylceramidase; P11947, Invitrogen) or 33 uM C12FDG (for beta galactosidase ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were detached by trypsinization and its activity was neutralized by Soybean trypsin inhibitor (Life technologies, Grand Island, NY). Culture conditions for HL-1 cells have been described previously.21 HL-1 cells were incubated with a MRCK inhibitor for 30 min at 37°C (BDP5290 ...
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bioRxiv - Biochemistry 2023Quote: NMA1982 phosphatase activity was measured using a standard fluorescence intensity phosphatase assay utilizing 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP; Invitrogen) as the substrate [26] ...
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bioRxiv - Evolutionary Biology 2023Quote: ... residual amidase activities were measured at A405 for 20 min using a Varioskan Flash multimode reader (Thermo Scientific, USA). One unit of amidase activity was defined as 0.00001 △A405/min.
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bioRxiv - Bioengineering 2023Quote: Cell viability and metabolic activity were measured with alamarBlue™ HS Cell Viability Reagent (Thermo Fisher Scientific, Waltham, MA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies were conjugated to horseradish peroxidase (HRP) and peroxidase activity was visualized using Chemiluminescent HRP substrate (Thermo Scientific). For quantification of protein samples ...
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bioRxiv - Immunology 2024Quote: ... Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific, Waltham, MA, USA). Densitometric analysis of immunoblots was done using ImageJ software.
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bioRxiv - Microbiology 2024Quote: ... and enzyme activity was normalized to protein concentration as determined by Pierce BCA protein quantification (Thermo Fisher Scientific, 23225) relative to a BSA standard.
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bioRxiv - Microbiology 2020Quote: ... The amplified DNA from one 96-well plate was normalized using a SequalPrep Normalization Plate (Invitrogen), and pooled into a single library ...
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bioRxiv - Bioengineering 2020Quote: ... or rectangular uniwell plates with overall dimensions of 96-well plates (Thermo Fisher Scientific, Waltham, MA) using custom-written software (see also below ...
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bioRxiv - Microbiology 2022Quote: ... Supplementary Table 3) and added (100 μL/well) to ELISA plates (Maxisorp Nunc-immuno plates; Thermo). Since Iwate/2019(H3N2 ...
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bioRxiv - Immunology 2022Quote: HLA-G+ EVTs were seeded on fibronectin-coated tissue culture plates (Nunc Cell-Culture Plates, ThermoFisher) at a density of 1 million cells/ml EVT culture medium ...
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bioRxiv - Molecular Biology 2020Quote: ... The assay was performed on 96-well plates (MicroAmp Fast 96-Well Reaction Plate, Applied Biosystems), in a total volume of 25 μL for each reaction ...
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bioRxiv - Immunology 2022Quote: HLA-G+ EVTs were seeded on fibronectin-coated tissue culture plates (Nunc Cell-Culture Plates, ThermoFisher) at a density of 1 million cells/ml EVT culture medium ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were prepared as follows in 384 well plates (MicroAmp Optical 184-Well Reaction Plate, Thermofisher): 5 µL Luna one-step reaction mix ...
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bioRxiv - Microbiology 2023Quote: ... with a collection plate (deep well storage plate, 96-well, 2,2 mL; Thermo Fisher Scientific, Germany).
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bioRxiv - Genetics 2021Quote: ... 2 ug were used as template for in vitro transcription with mMESSAGE mMACHINE T7 ULTRA Transcription Kit (Ambion), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The two tceA clone libraries were generated using the TOPO™ XL-2 Complete PCR Cloning Kit (Invitrogen). PCR primers were designed to amplify the 2200 bp tceAB region (Table S1) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 µg of total RNA from HEK293 cells or tissue samples was treated with DNAseI Free kit (Thermofisher) followed by cDNA generation using the High capacity cDNA kit (Thermofisher) ...
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bioRxiv - Genomics 2020Quote: ... according to manufacturer instructions and subjected to 2 rounds of DNA digestion using the DNA-free kit (Ambion) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... for 2 h at 55°C and DNA was purified using DNA clean-up kit (Thermo Fisher Scientific). DNA fragmentation range was assessed by electrophoresis.
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bioRxiv - Genetics 2019Quote: ... Complementary DNA was synthesized from 2 μg total RNA using a High Capacity cDNA Reverse Transcription Kit (Invitrogen). Primers were designed using Primer Premier Software (Primer Premier 5.0) ...
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bioRxiv - Immunology 2021Quote: Approximately 2 × 106 cells were fractionated using the Subcellular Protein Fractionation Kit for Cultured Cells (Thermo scientific, #78840).
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bioRxiv - Bioengineering 2021Quote: ... 2 μg of total RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Invitrogen, Brazil). The obtained cDNA was used as a template for gene amplification using specific primers ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized from 2 μg RNA by either the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) or the iScript Reverse Transcription Supermix (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... SARS-CoV-2 titration was obtained using a TaqMan 2019nCoV assay kit v1 RealTime-PCR (Thermo Fisher, Italy).
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from 2 µg of RNA using High Capacity cDNA RT kit (Applied Biosystems, cat#4368814). qPCR was performed in a CFX96 Real-time system (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... First-strand cDNA was synthesized from 2 µg of RNAusing High-Capacity cDNA Reverse Transcription Kits (Applied Biosystems, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... 0.5 to 2 μg of RNA was reverse transcribed with the high-capacity cDNA RT kit (Applied Biosystems), using random hexamers ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 μg of RNA was reverse transcribed into cDNA using the high-capacity reverse transcription kit (Applied Biosystems). cDNA and relevant primers were added to PowerUp SYBR green master mix (Applied Biosystems ...