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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... cDNA was synthesized from 2 µg RNA using the High-Capacity cDNA Reverse Transcriptase Kit (Thermo Scientific). 20 µL cDNA reactions were diluted 1:10 in UltraPure water ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µl of purified RNA was reverse transcribed using the SuperScript IV Reverse Transcriptase Kit (Invitrogen; 18090050) with random hexamer priming ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were assayed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™, Thermo Fisher Scientific, cat. no. V13154). Cells were plated in triplicate in a 96-well plate (5,000 cell/well) ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were determined using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™, Thermo Fisher Scientific, cat. no. V13154). Cells were plated in triplicate in a 96-well plate (3,000 cell/well in 200 μL of complete medium and cultured under standard conditions for 48 h ...
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... The plates were read at 450 nm on a VarioSkan Plate Reader (Thermo Fisher Scientific) within 10 minutes of adding stop solution.
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bioRxiv - Immunology 2021Quote: ... The plates were read at 450 nm on a VarioSkan Plate Reader (Thermo Fisher Scientific) within 10 minutes of adding stop solution.
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bioRxiv - Microbiology 2019Quote: ... Ninety-six-well plates (MicroAmp EnduratePlate Optical 96-well fast clear reaction plates; Applied Biosystems) were used and heated from 25 to 95°C with a ramp rate of 0.05°C/s and read by a thermocycler (QuantumStudio 5 real-time PCR system ...
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bioRxiv - Molecular Biology 2020Quote: ... Each well of the microtiter plate (MicroAmp fast optical 96-well reaction plate, Applied Biosystems) was filled with 20 µL of final solution ...
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bioRxiv - Neuroscience 2022Quote: Plates were transported from plate hotels using a CRS CataLyst Express robot from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: Assay plates (Nunc™ MaxiSorp™ 96-well polystyrene plate catalog #449824; Thermo Fisher Scientific) were coated with 100 μL/well of the capture antibody (2B7 or anti-PP2A ...
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bioRxiv - Microbiology 2023Quote: ... Cells were cultured in 96-well plates using a spectrophotometer plate reader (Thermo Fisher Scientific) with antibiotic concentrations ranging from 0.25 μg/mL to 2048 μg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... The plate absorbance was measured by a plate reader at 450nm (Multiscan Sky, Thermo Scientific). The number of epitopes was calculated by comparison with a calibration line obtained using rabbit red blood cells [27].
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bioRxiv - Bioengineering 2023Quote: ... The plate absorbance was measured by a plate reader at 450nm (Multiscan Sky, Thermo Scientific).
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bioRxiv - Plant Biology 2023Quote: After coating of the 96-well plates (Nunc-Immuno® Plates, Thermo Scientific, Waltham, USA) with 100 µl per well of the sample in triplicate in different concentrations in ddH2O (12.5 µg mL-1 for AmOx and KOH ...
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bioRxiv - Immunology 2024Quote: ... The plate was analyzed every hour using a Multiskan FC plate reader (Thermo Fisher Scientific), and absorbance was measured at 620 nm.
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bioRxiv - Immunology 2024Quote: ... All plates were read on a MultiSkan Sky ELISA plate reader (ThermoFisher Scientific MA USA) at 492 nm ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM Tris(2-carboxyethyl)phosphine (TCEP, ThermoFisher)) supplemented with a protease inhibitor tablet (Roche ...
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bioRxiv - Genetics 2019Quote: ... 2 μl of probe (2 μM; Applied Biosystems) and 2 μl of template DNA (where extracts were normalised to 34 ng/μl) ...
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bioRxiv - Molecular Biology 2023Quote: ... cell culture plates (12-well plates were Corning™ Costar™ cat# 3513 and 6-well plates were Thermo Fisher Scientific Nunc™ cat# 140675) were coated with GeltrexTM (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... cell culture plates (12-well plates were Corning™ Costar™ cat# 3513 and 6-well plates were Thermo Fisher Scientific Nunc™ cat# 140675) were coated with GeltrexTM (Gibco ...
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Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... Clones were scraped off the LB plates and plasmid DNA was extracted using PureLink™ HiPure Plasmid Maxiprep Kit (ThermoFisher, K210007). The libraries were submitted for next generation sequencing to confirm the coverage and diversity of gRNA and shRNA libraries ...
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bioRxiv - Genomics 2020Quote: ... Cells were sorted with a BD Influx Sorter into 96-well plates and lysed using the Arcturus PicoPure Kit (Applied Biosystems) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... Transformed cells were recovered in S.O.C media (from ElectroMAX Stabl4 kit) for 1h and spread on Luria–Bertani agar plates (245×245 mm, Thermo Fisher Scientific) containing 100 µg/mL ampicillin ...
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bioRxiv - Microbiology 2022Quote: HK1 grown in 100 mm plates were transfected with 5 μg of RFP-LMP1 or GFP-PD-L1 plasmid using Lipofectamine 3000 transfection kit (Invitrogen, L3000015). Lipofectamine reagents were diluted in Opti-MEM medium (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Total protein content was determined by the Lowry method (77) adapted to a 96-well plate assay with the Microplate BCA Protein Assay Kit (23252, Thermo Scientific), using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2020Quote: Single cells were sorted in 96-well PCR plates in 9 µl of RT/pre-amp mix from the CellsDirect One-Step qRT-PCR Kit (Life Technologies) and were kept at −80°C at least overnight ...
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bioRxiv - Microbiology 2021Quote: ... The resulting amplification products were verified by gel electrophoresis and cleaning and normalization of individual PCR products was performed with SequalPrep™ Normalization Plate (96) Kit (Invitrogen). The normalized PCR amplicons were mixed ...
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bioRxiv - Microbiology 2019Quote: ... The DNA amplicons in each PCR reaction was normalized using the SequalPrep Normalization Plate Kit (Thermo Fisher Scientific, catalog no. A1051001). The normalized reactions were pooled and quantified using the Kapa Biosystems Library qPCR MasterMix (ROX Low ...
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bioRxiv - Cell Biology 2019Quote: ... The incubation and cycling conditions were set as described in the kit and the plates were analysed in a StepOnePlus Real-Time PCR System (Thermo Scientific).
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bioRxiv - Pathology 2021Quote: ... Cells grown for 24h in 6-well plates were transfected with 1 μg of cDNA construct (Asic2b or t-Asic2b) using a Lipofectamine™ Plus kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Samples were quantified in a 96-well plate reader using the Quant-IT DNA high sensitivity assay kit (Invitrogen, Cat #Q33232) according to the manufacturer’s protocol and pooled into 1 tube ...
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bioRxiv - Immunology 2020Quote: ... or luminal cells collected in vaginal washes were plated in 96-well plates and incubated with Live/Dead Fixable Aqua Dead Cell Stain kit (Molecular Probes) for 15 minutes at room temperature (RT ...
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bioRxiv - Genomics 2021Quote: Protein lysates derived from same plates used for metabolomics collection were assayed for total protein concentration with Pierce BCA Protein Assay Kit (ThermoFisher 23225) for normalizing raw metabolite values ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were plated into black 96-well clear bottom plates and analyzed using CyQuant cell proliferation assay kits (Thermo Scientific, c7026).
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bioRxiv - Immunology 2022Quote: ... Amplified linear DNA cassettes encoding monoclonal heavy and light chain genes were co-transfected into Expi293 cells in 96-well deep-well plates using the Expi293 Transfection Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... A volume of 200 μL of milk samples were used as starting material for magneticbased DNA extraction using a 96-well plate and CORE kit in a KingFisher instrument (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately two million colonies were scraped from plates and pooled and plasmid DNA was purified using PureLink® HiPure plasmid filter Midiprep kit (Invitrogen). The resulting plasmid library was transformed into TRy1392 pdr5∆ yeast strain ...
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bioRxiv - Neuroscience 2023Quote: Calcium flux was measured in MNs seeded in a 96 well plate using the Fluo-4 NW Calcium Assay Kit (ThermoFisher, #F36206) per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: RNA was extracted from 8-μm snap frozen sections of distal femur and proximal tibial growth plates in Axin2CreERT2;R26RTdTomato mice using the Picopure RNA Isolation Kit (KIT0204, Thermo Fisher) as previously described.56 Microdissections of resting chondrocytes and proliferative chondrocytes were performed using a Leica LMD 7000 laser microdissection system (Leica Microsystems) ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: Calcium flux was measured in MNs seeded in a 96 well plate using the Fluo-4 NW Calcium Assay Kit (ThermoFisher, #F36206) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Cells were seeded in 96 well plates (6055302, Revvity) and stained with Blue/Green ReadyProbes Cell Viability Imaging Kit (Invitrogen, R37609) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 20 clones from each plate were expanded and genomic DNA extracted by a GeneJet Genomic DNA Purification Kit (ThermoFisher Scientific, K0722) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 (Thermofisher). A reverse transfection approach was used to deplete AurkA and CEP135 ...
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bioRxiv - Systems Biology 2023Quote: ... 2% (ThermoFisher). Purified amplicons were quantified by Qubit™ dsDNA Quantification Assay Kits (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected with the EGFP-Caspase 2 or EGFP-Δ Caspase 2 plasmid and the synthesized miR-2 (miR-2-mimic) using Cellfectin II transfection reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Macrophages were incubated with the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (10 μM, Invitrogen, California, USA) in PBS for 30 min ...