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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were lysed in 96 well plates and RNA was extracted using Applied Biosystems MagMax 96 total RNA isolation kit (Thermo Fisher, # AM1830) with on bead DNase digestion ...
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bioRxiv - Biochemistry 2021Quote: ... A positive clone was selected from Petri-plates and then plasmid from positive clone was extracted using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). The integrity of cloned gene in the pET28a in terms of orientation and sequence was checked at this stage ...
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bioRxiv - Immunology 2020Quote: ... Each plate was pooled and the plate libraries were quantified on a Qubit Fluorometer 3.0 using a dsDNA High-sensitivity Kit (Life Technologies, Carlsbad, CA). The plates were then pooled in equal volumes and the pooled library was then diluted to 6 μM and sequenced using a 500-cycle V3 reagent on an Illumina MiSeq (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Amplicon sizes were determined using an Agilent 2200 TapeStation system (∼550bp) and libraries normalized using SequalPrep Normalization Plate Kit (Thermo Fisher Scientific). Library concentration was calculated using a SYBR green quantitative PCR (qPCR ...
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bioRxiv - Physiology 2023Quote: All real-time PCRs were carried out in 96-well plates using ChamQ SYBR qPCR Master Mix kit in the ABI Prism 7500 Sequence Detection System (Applied Biosystems, USA) at 95°C ...
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bioRxiv - Biochemistry 2023Quote: ... All assays were performed on a 100-μl scale in a 96-well plate according to the “Amplex™ Red Cholesterol Assay Kit” protocol (ThermoFisher Scientific). No cholesterol esterase was added ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted in a 96-well format from the consolidated Prospector® culture plates using the Extract All Kit (Applied Biosystems). 20µL of culture was combined with 20µL of Lysis Solution and incubated for 10 minutes at 95°C ...
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bioRxiv - Biochemistry 2023Quote: ... The amount of lactate in the reaction mix was measured with the Lactate Assay Kit (colorimetric assay) by reading the absorbance using Multiskan SkyHigh plate reader (Thermo Fisher Scientific). Specifically ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of SAGs were transferred to replica plates for DNA yield quantification using Quant-iT dsDNA Broad-Range (BR) Assay Kit (Thermo Fisher Scientific) prior to library preparation ...
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bioRxiv - Developmental Biology 2021Quote: ... 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]- D-glucose (2-NBDG) (Invitrogen N13195) was used as a probe for monitoring glucose uptake ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... the plate was then read at 450 nm using a Multiskan® EX plate reader (Thermo Scientific), and the data analysed in GraphPad Prism version 7.0c ...
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bioRxiv - Bioengineering 2021Quote: ... Fluorescence readings were conducted using a Spectramax plate reader in a black-walled clear bottom plate (Nunc), with the excitation/emission for TO1 and TO3 being 510/535 nm and 637/658 nm ...
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bioRxiv - Microbiology 2021Quote: ... pneumoniae (one colony per species per plate) were further sub-cultured on Sheep Blood Agar plates (ThermoFisher) and species identity confirmed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were coated onto the ELISA plate (Nunc-Immuno™ plate; Thermo Fisher Scientific, Roskilde, Denmark). The antigen-coated wells were then blocked with 20% Blocking One (Nacalai Tesque ...
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bioRxiv - Neuroscience 2021Quote: ... the plate was washed 4 times with PBST using a plate washer (Thermo Fisher Scientific Wellwash Versa), and anti-pTRKB antibody (1:1000 in the blocking buffer ...
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bioRxiv - Plant Biology 2023Quote: Stratified seeds were germinated on square plates (100 mm × 100 mm square plates; Fisher Scientific; Cat#FB0875711A) containing sterile full-strength (1x ...
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bioRxiv - Microbiology 2023Quote: ... the plate was transferred to 20° C pre-cooled plate reader (Multiskan GO, Thermo scientific, Waltham, MA) and shaken slowly for 10 seconds ...
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bioRxiv - Biophysics 2023Quote: 6-well plate cell proliferation – Cells were seeded into 6-well plates (Fisher Scientific, 07-200-83) 150,000 cells/well and left at ambient temperature for 20 minutes to ensure homogeneous settling ...
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bioRxiv - Biochemistry 2024Quote: ... if a 96-well plate (SureSTARTTM WebSealTM 96-Well Microtiter Plate, SN: 60180-P210B, Thermo Fisher Scientific) was used for injection ...
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bioRxiv - Physiology 2022Quote: Maximal enzyme activity of citrate synthase was determined from muscle homogenate using fluorometry (Fluoroscan Ascent, Thermo Fisher Scientific, Waltham, USA) at 25°C as previously described (Lowry ...
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bioRxiv - Cell Biology 2022Quote: ... the metabolic activity of viable cells was tested by a colorimetric dimethyl-thiazole-diphenyltetrazolium bromide (MTT) assay (cat. no. M6494, Invitrogen). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA fragments resulting from nuclease activity during the Surveyor assay were separated by electrophoresis in 10% TBE-polyacrylamide gels (ThermoFisher). A quantitative measurement of optical density through Image J was performed to quantify efficiency of CRISPR/Cas9 editing ...
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bioRxiv - Immunology 2022Quote: ... TG2 enzymatic activity was measured using 5-BP crosslinking and was visualized by costaining with AF594-conjugated streptavidin (Invitrogen, S32356). Images were acquired at 20x magnification using a Leica SP8 Laser Scanning Confocal microscope ...
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bioRxiv - Cell Biology 2022Quote: ... The metabolic activity of the cells was quantified by treating cells with the PrestoBlue Cell Viability Reagent (Invitrogen, catalog # A13262) for two hours and measuring the optical density at 570 nm per manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Proteins were visualized by chemiluminescent detection (Image Quant) of peroxidase substrate activity (SuperSignal West Femto Maximum Sensitivity Substrate, Thermo Scientific).
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bioRxiv - Bioengineering 2020Quote: ... hMSCs cultured on flat and topographically-textured microparticle discs were also assessed for metabolic activity using PrestoBlue™ (Invitrogen, UK) at 4 hours post-seeding (for assessment of initial cell attachment ...
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bioRxiv - Cancer Biology 2022Quote: ... Luciferase activity was normalized to the number of viable cells measured by a PrestoBlue cell viability reagent (ThermoFisher Scientific, A13261). When using TOPFlash or FOPFlash for TCF/LEF-luciferase reporter assays ...
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bioRxiv - Plant Biology 2023Quote: ... HA and LexA fusion proteins were detected by HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... Trypsin activity was stopped by diluting 1+1 with a 1 mg/mL solution of soy bean trypsin inhibitor (#10684033 Gibco) in PBS ...
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bioRxiv - Biochemistry 2023Quote: The phosphatase activity of SHIP1 and SHIP2 was determined by measuring inorganic phosphate production using Phosphate Sensor (ThermoFisher Scientific, PV4407). Soluble inositol 1,3,4,5-tetraphosphate (I(1,3,4,5)P4 ...
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bioRxiv - Biochemistry 2023Quote: To study the effect of PfRKIP on the transphosphorylation activity of PfCDPK1 we used Pro-QTM diamond stain (ThermoFisher Scientific). PfCDPK1 was incubated with different concentrations PfRKIP (2 μg-16 μg ...
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bioRxiv - Immunology 2023Quote: ... PCLS containing bronchia and showing 100% ciliary activity were selected by light microscopy (EVOS FL Auto; Thermo Fisher Scientific, USA) for subsequent experiments ...
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bioRxiv - Biochemistry 2023Quote: hBMSC-TERT were plated at a density of 20 000 cells/cm2 (Alizarin Red S staining) and 5 000 cells/well (ALP activity assay) in MEM medium (Gibco), supplemented with 10 % FBS (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... The measurement of lysosomal pH and Cathepsin B activity was evaluated with LysoTrackerTM Red DND-99 probe (InvitrogenTM, ThermoFisher Scientific) and the Magic Red® kit (Immunochemistry Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Transfections into NRK and COS-7 cells for use in fixed or live imaging experiments and GST-RILP Rab7 activity pulldowns were performed using Lipofectamine 2000 (Invitrogen) per manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were inoculated at the top of a test tube containing agar and tributyrin for lipolytic activity measure (10 mL.L-1, ACROS Organics) or semi-skimmed cow milk for the proteolytic activity measure (40 g.L-1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 to 2 µg of RNA was reverse transcribed with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) using random hexamers for amplification of cellular genes or gene specific primers for amplification of either minus or plus strand viral RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized from 2 μg of total RNA using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). The expression of 370 ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA (2 µg) was then reverse transcribed with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems; ThermoFisher Scientific). RT-qPCR was then performed via the CFX384 Real Time system (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA (2 µg) was then reverse transcribed with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems; ThermoFisher Scientific). RT-qPCR was then performed via the CFX384 Real Time system (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µg of the extracted RNA were reverse-transcribed using the high capacity cDNA reverse transcription kit (Applied Biosystems) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... using a 1:2 mass ratio of light and heavy chain DNA with the Expifectamine transfection kit (Life Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 0.5 to 2 µg of RNA was reverse transcribed with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) using random hexamers for amplification of cellular genes or gene specific primers for amplification of either minus or plus strand viral RNA ...
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bioRxiv - Molecular Biology 2019Quote: ... Reverse transcription was performed using first-strand cDNA synthesis kit containing SuperScriptIII® (2 units/µL; Invitrogen, Life Technologies).
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bioRxiv - Molecular Biology 2019Quote: ... Reverse transcription was performed using first-strand cDNA synthesis kit containing SuperScriptIII® (2 units/µL; Invitrogen, Life Technologies).
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bioRxiv - Bioengineering 2021Quote: ... DNA quality was assessed using a 2% agarose gel and purified using the GeneJet PCR kit (Thermo Fisher Scientific). DNA was then eluted to a concentration of between 25-100 ng/µL and amplified using the T7 MegaShortScript RNA synthesis kit (Ambion ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed with 2 μg RNA per sample using RevertAid First Strand cDNA Synthesis kit (Thermo Fisher). PR1 expression was detected using primers qPR1_F and qPR1_R by quantitative real-time PCR using Maxima SYBR green mix (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... Detection of EdU (5-ethynyl-2’-deoxyuridine) was performed as per the manufacturer’s guidelines using Click-it EdU Alexa Fluor 488 imaging kit (Invitrogen).