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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for CD8 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The sorted CD8+ cells were then cultured in RPMI-160 medium supplemented with 10% FBS and activated with anti-CD3 antibody (Thermo Fisher Scientific), anti-CD28 antibody (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: Naive CD8 T cells were isolated from spleen and LN using two rounds of negative depletion with Streptavidin DynaBeads™ (Thermo Fisher) using 1*108 and 4*107 beads per 1*108 total splenocytes/LN cells ...
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bioRxiv - Immunology 2024Quote: ... mice were intravenously injected with 1x106 (optimal) or 0.5x106 (suboptimal) activated OTI CD8+ T cells in 100 μL of sterile PBS (ThermoFisher Scienfic, ref 10010023).
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bioRxiv - Immunology 2024Quote: ... we co-transfected 3×106 3-day activated CD8+T cells with 150 pmol/106 cells of human TSP-4-specific siRNAs (#s14100, Thermo Fisher Scientific) and 1.5 μg/106 of the pMax-TSP-1-GFPSpark construct ...
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bioRxiv - Neuroscience 2019Quote: ... and rabbit (1:500 goat anti-rabbit, ThermoFisher A11012) conjugated with two-photon-excitable fluorophores (488nm and 594nm ...
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bioRxiv - Microbiology 2024Quote: ... or rabbit (Donkey anti-rabbit AF594, Invitrogen, Cat# A11012). Cells were washed in 1x PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-TRF1 antibody (Goat Pab sc-1977, SantaCruz), or anti-PAR (Mouse Mab 10H ALX-804220, Alexis) recovered with Protein-G dynabeads (Invitrogen), run on PAGE together with input sample (1:20 of amount of immunoprecipitated proteins ...
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bioRxiv - Immunology 2022Quote: ... we labeled the gp120-specific antibody (A32 mAb) with Allophycocyanin (APC) following the manufacturer instructions (Zenon Human IgG labeling kit, Invitrogen) and measured cell binding by flow cytometry ...
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bioRxiv - Microbiology 2022Quote: ... anti-mouse surface antigen 1 (SAG1) mab DG52 1:500 46 and then with goat anti-rat-Alexa546 1:300 (Invitrogen), goat anti-rat-Alexa375 1:300 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... The purified mAbs were immediately passed over a custom made rhTNFα affinity column (NHS-activated agarose beads, Thermo Fisher Scientific) in gravity mode ...
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bioRxiv - Neuroscience 2020Quote: ... 1 ml of Dynal sheep-anti mouse IgG paramagnetic beads were non-covalently coupled with 60 µg of anti-His mAb (Invitrogen) in PBS plus 0.1% immunoglobulin-free BSA (Sigma ...
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bioRxiv - Pathology 2021Quote: Recombinant Fab fragments were produced in HEK cells by introducing a stop codon at the end of CH1 and purified by affinity chromatography on AKTA Xpress Mab System (Cytiva) with UNICORN 5.11 software version (Build 407) using CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific), buffer exchanged to PBS using a HiPrep 26/10 desalting columns (Cytiva) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: After SDS-PAGE and Western blotting the membranes were probed with anti-NP MAbs R001 and 1C7 at 1:1000 diluted in Superblock Buffer (ThermoFisher). Then antigen-antibody complexes were detected using appropriate anti species HRP-conjugates and West Femto substrate.
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bioRxiv - Microbiology 2022Quote: ... non permeabilized samples were incubated with an anti-SAG1 mAb followed by Alexa 488-conjugated anti-mouse IgG antibodies (Invitrogen). After permeabilization with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... used at 1:5,000 in MAB buffer (100 mM Maleic acid, 150 mM NaCl, and pH 7.5) + 20% heat-inactivated lamb serum (Gibco 16070096) + 2% blocking reagent (Sigma 11096176001) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins bound to GP96 were immunoprecipitated with an antibody against Grp94 (Rat, mAb, Enzo) and Dynabeads Protein G (Thermo Scientific), then examined using mass spectrometry analysis ...
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bioRxiv - Microbiology 2020Quote: ... Cleavage of junctional proteins was detected by Western blot analysis using specific antibodies against E-cadherin (Mouse, mAb, Thermo Scientific), p120-catenin (Rabbit ...
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bioRxiv - Microbiology 2021Quote: ... with the following changes: goat anti-rat and streptavidin Molecular Probes Alexa-Fluor-488 and 555 conjugated secondary mAbs (Invitrogen) were used at 4µg/ml ...
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bioRxiv - Immunology 2020Quote: ... The half-maximal inhibitory concentrations (IC50) of the evaluated mAbs were tested by the Varioskan LUX Microplate Spectrophotometer (Thermo Fisher), and calculated by a four-parameter logistic regression using GraphPad Prism 8.0.
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bioRxiv - Microbiology 2021Quote: ... The sequencing of the selected mAbs was performed according with the protocol described in the commercial kit BigDye Terminator v3.1 (Life Technologies), and for that purpose the same primers described for PCR were used ...
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bioRxiv - Microbiology 2019Quote: ... paired heavy and light chain plasmids for each mAb were expressed in Expi293F cells (Cat# A14527, ThermoFisher Scientific, Waltham, MA) in a 96-well format ...
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bioRxiv - Immunology 2021Quote: ... was performed overnight with 500 µl/well of sterile ddH2O supplemented with 1 µg/ml anti-CD3 mAb (clone OKT3, Invitrogen) and 1 µg/ml anti-CD28 mAb (clone CD28.2 ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone) primary mAb and FITC goat anti-mouse secondary antibody (Invitrogen). The cells were then fixed using 4% paraformaldehyde before staining the nuclei with Hoescht 33342 (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... The excess biotin of biotinylated mAbs was removed with 7k molecular weight-cutoff (MWCO) Zeba spin desalting columns (Thermo Scientific). Plates were coated with 2 μg/ml RBD antigen overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: The Fab fragments of anti-Muc1 mAbs 14A and 16A were expressed by Bac-to-Bac baculovirus expression system (Invitrogen). The heavy-chain (Hc ...
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bioRxiv - Microbiology 2022Quote: ... Surface PCDH1 was stained using human anti-EC7 mAb-3677 (5 µg/mL) followed by anti-human Alexa FluorTM 555 (ThermoFisher) for 1 h at 4°C each ...
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bioRxiv - Immunology 2024Quote: ... Bound mAb was detected using goat anti-human (H+L)-HRP (1:1000 dilution) (Thermo Fisher Scientific, Waltham, MA, USA). In the case of His-tag detection ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked for 1h at room temperature with blocking buffer (3% skim milk powder dissolved in tris-buffered saline supplemented with 0.1% Tween-20 [TBS-T]) and then probed with CV3-25 mAb (1ug/mL) followed by HRP-conjugated anti-human IgG (1:3000, Invitrogen). HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were incubated with 5 μL of anti-SAG1 mAb DG52 and 50 μL of Dynabead protein G magnetic beads (Invitrogen) at 4 ℃ for 2 hr ...
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bioRxiv - Immunology 2024Quote: ... and resuspended in perm/wash buffer containing 1 μg of mAb conjugated with Zenon 647/ 488 IgG1 (ThermoFisher Scientific, USA) for 30 min in the dark on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... used at 1:5000 in MAB buffer (100 mM Maleic acid, 150 mM NaCl, and pH 7.5) + 20% heat-inactivated lamb serum (Gibco 16070096) + 2% blocking reagent (Sigma 11096176001) ...
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bioRxiv - Microbiology 2023Quote: ... Fixed cells were first incubated with a mouse anti-E-cad cytoplasmic domain mAb (1:1000) (4A2C7, Life Technologies, USA) for 1 h at room temperature and then with 1:500 rabbit polyclonal anti-C ...
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bioRxiv - Microbiology 2023Quote: ... β-actin expression was measured using an anti-human β-actin horseradish peroxidase-conjugated mAb (1:25000, Life Technologies, USA) as the loading control ...
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bioRxiv - Neuroscience 2023Quote: ... Final sample purity and OTR ratio was confirmed by reverse phase LC/MS (Mab pac RP, Thermo Fisher Scientific, #088644) with mobile phase A ...
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bioRxiv - Molecular Biology 2023Quote: ... and immediate collection of bead bound RNA and transcription complexes in Trizol following immunoprecipitation by Rpb1 CTD (4H8) mouse mAB conjugated to Dynabeads M-280 sheep anti-mouse IgG (ThermoFisher). mNET-seq experiments were completed in biological replicate ...
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs were seeded onto 6-well plate at density 2 × 106 cells/mL in full RPMI-1640 medium and stimulated for 48h with anti-CD3 (1:1000) and anti-CD28 (1:1000) mAbs (Invitrogen). After 48h ...
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bioRxiv - Cancer Biology 2024Quote: ... The purification of bispecific antibodies was done by affinity chromatography on a mAb SelectSure resin (Cytiva) followed by a first polishing step by affinity chromatography on a CaptureSelect LC Lambda resin (Thermofisher) and a second polishing step by size exclusion chromatography ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were also probed with a mouse monoclonal antibody targeting glyceraldehyde-3-phosphate dehydrogenase (mAb-α-GAPDH; 1:8000; ThermoFisher).
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Fezf2 and rabbit anti-involucrin were revealed with Cy3-conjugated anti-rabbit IgG (Invitrogen) and biotinylated anti-TPA was revealed with Alexa Fluor 488-conjugated streptavidin (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... and CD8+ T cells (OT-I) were purified from TCR transgenic mice were purified by sorting and labeled with CTV (Invitrogen, ThermoFisher, Waltham, MA) (as per manufacturer's protocol) ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with human MR1-5-OP-RU tetramer for 40 minutes at RT in the dark prior to the addition of anti-bovine CD8-biotin (Clone ILA51) and Near-Infrared Fixable LIVE/DEAD stain (Invitrogen or Molecular Probes) for 20 minutes at 4 °C ...
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bioRxiv - Immunology 2019Quote: Spleen cells from 5 month animals were sorted for CD8 positive cells and cDNA was prepared using SuperScript III First-Strand Synthesis System for RT-PCR from Invitrogen (cat # 18080-051). The cDNA was then used in the TCRExpress™ MouseTCRVβ screening kit (BioMed Immunotech ...
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bioRxiv - Immunology 2021Quote: Secreted IFNγ was analyzed using CD8+ T cells (5 × 104) that were stimulated with α-CD3ε and α-CD28 conjugated beads (Life Technologies). IFNγ was detected with the BD OptEIA™ Mouse IFNγ ELISA Set (BD Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... Proliferation of CD4+ and CD8+ T cells was induced by stimulating cells with plate bound anti-CD3 [0.1 µg/mL] (Thermo Fisher Scientific, MA, USA) and soluble anti-CD28 [1 µg/mL] (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD3 PE, Life Technologies; CD8 FITC, Life Technologies; CD11b AF700, Life Technologies CD19 PerCP-Cy5.5 ...
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bioRxiv - Neuroscience 2022Quote: ... brains were fixed for 14 min in freshly prepared 4% paraformaldehyde and incubated overnight in rat anti-CD8 (1:50, Thermo Fisher #MA5-17594) and mouse nc82 (1:40 ...
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bioRxiv - Genomics 2024Quote: CD4+ and CD8+ T cells were extracted from the lymphocyte fraction using the Dynabeads CD4 Positive Isolation Kit and Dynabeads CD8 Positive Isolation Kit (Invitrogen, Waltham, MA, USA) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: Plasmids encoding for recombinant His-tagged proteins were transformed into Escherichia coli BL21(DE3) (Invitrogen). Bacterial cultures were grown at 37°C in LB media to OD600 of 0.6-0.8 and induced with 0.5 mM IPTG at 18 °C for 16h (overnight) ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then stimulated with 20 ng/mL of recombinant human IL-4 (Gibco) and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich ...