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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for CD8 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The resin was subsequently washed with 3 resin volumes (RV) of the same buffer before mAb P3.2 (Thermo Fisher) was eluted using 3 RV of 0.2 M glycine pH 2.5 ...
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bioRxiv - Microbiology 2021Quote: ... COV2-2196 and COV2-2130 mAbs were produced after transient transfection using the Gibco ExpiCHO Expression System (ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... used at 1:5,000 in MAB buffer (100 mM Maleic acid, 150 mM NaCl, pH7.5) + 10% heat-inactivated lamb serum (Gibco 16070096) + 2% blocking reagent (Sigma 11096176001) ...
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bioRxiv - Microbiology 2020Quote: ... all mAbs were made as IgG1 and expressed in Chinese hamster ovary cells (ExpiCHO; Thermo Fisher Scientific, Waltham, MA). The mAbs were purified using HiTrap Protein A HP column (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed with TBS twice before incubating with serially diluted mAbs in casein blocking buffer (Thermo Fisher Scientific) for 90 min ...
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bioRxiv - Immunology 2020Quote: ... PE-Cy7-conjugated anti-human CD196 (CCR6) and PE-Cy7-conjugated anti-human MAVS mAbs were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Immunology 2020Quote: ... and α-CD28 (clone 9.3) mAbs at a concentration of 1 μg/ml in RPMI medium 1640 (Life Technologies) supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Cell Biology 2022Quote: Endogenous TGF-βRII was probed using by two monoclonal antibodies (mAbs: sc-17792, Santa Cruz, and PA5-36115, ThermoFisher). Neither detected endogenous protein in our immunofluorescence or western blot studies.
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bioRxiv - Immunology 2022Quote: ... The supernatant containing secreted mAb was harvested at day 4 and purified using protein A-agarose beads (Thermo Fisher) as detailed previously56.
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bioRxiv - Microbiology 2024Quote: ... directed against the cytoplasmic domain of E-cad and a monoclonal anti-Occludin mouse mAb (331500, Life Technologies SA) and revealed using an anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2024Quote: ... mAbs binding to the S proteins were detected using 25 µl/well of PNPP (p-nitrophenyl phosphate; Thermo Fisher) and the reaction was measured at a wavelength of 405nm using the Varioskan Lux Reader (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... The mAbs were detected using Alexa Fluor-594 conjugated goat anti-mouse IgG (H+L) (Invitrogen Cat# A-11032) and the reference Ab with an Alexa Fluor-488 conjugated goat Ab against the appropriate host (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Certain mAbs were loaded with 50 μL/well to 96 well microplates (Thermo fisher maxisorp, catlog No: M9410-1CS) and incubated overnight After three washes with PBS-T (PBS with 0.05% Tween 20) ...
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bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant baculovirus was generated using the Bac-to-Bac baculovirus expression system (Fisher Scientific) and was produced in Sf9 cells ...
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bioRxiv - Immunology 2021Quote: ... A recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... linearized recombinant pCS2+ plasmids using the mMESSAGE mMACHINE SP6 transcription kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... A recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 20 ng/mL human recombinant basic fibroblast growth factor (bFGF; Invitrogen, USA), 20 ng/mL epidermal growth factor (EGF ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant antibody was labelled with Alexa Fluor 594 following the manufacturer’s instructions (Life technologies). Standard molecular biology techniques were used to introduce a cysteine residue at position 239 in the CH2 domain of each heavy chain (44) ...
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bioRxiv - Microbiology 2019Quote: ... and 5 μg.L−1 of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (Wu et al ...
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bioRxiv - Genetics 2019Quote: ... cells were incubated in differentiation medium (DM) (10 µg/ml recombinant human insulin (Gibco), 2% HI horse serum (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 µg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant baculovirus was produced using the Bac-to-Bac Baculovirus expression system (Thermo Fisher). The mouse utrophin (NP_035812 ...
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bioRxiv - Bioengineering 2021Quote: Recombinant baculovirus were generated by using a Bac-to-Bac expression system (Invitrogen, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Gibco™ FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2 ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant protein was expressed in Expi 293 GnTI- (Thermo Fisher, Waltham, MA, USA) at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant cells were selected using 150 μg/ml of Hygromycin B (Invitrogen, Paisley, U.K.).
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bioRxiv - Cell Biology 2021Quote: Purified recombinant GST-tagged CDK1-cyclin A2 and His6-tagged CDK1-cyclin B1 (Invitrogen) were stored in 150 mM NaCl ...
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bioRxiv - Cancer Biology 2022Quote: ... or by tryptic shaving of cell-surface proteins by using recombinant enzymes (TrypLE, Gibco). Three biological replicates of cells cultured in the presence or absence of serum ...
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bioRxiv - Biochemistry 2022Quote: ... recombinant baculoviruses were generated according to the Bac-to-Bac Baculovirus expression system (Invitrogen). 2 ml of P3 virus were used to infect 1 L of Sf9 insect cells at a density of 1.1×106 ml-1 ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant baculoviruses were generated through the Bac-to-Bac system (ActRIIA; Invitrogen - Waltham, MA) or the Baculogold system (ActRIIB ...
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bioRxiv - Cancer Biology 2021Quote: ... in the presence of RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher, Cat. No. 10777019). Following 10 minutes ice-incubation and centrifugation at 21,000 x g for 1 minute ...
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bioRxiv - Microbiology 2021Quote: ... A recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... in the presence of RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher, Cat. No. 10777019). Following 10 minutes incubation on ice and centrifugation at 21,000 x g for 1 minute ...
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bioRxiv - Cell Biology 2022Quote: ... Basal Medium plus 50 ng/ml human recombinant VEGFA (PHC9391 Gibco, Frederick, MD, USA) or Basal Medium plus 100 ng/ml recombinant human NTN1 (6419-N1 ...
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bioRxiv - Microbiology 2019Quote: ... Strains used for cloning and expression of recombinant proteins were Escherichia coli TOP10 (Invitrogen) and E ...
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bioRxiv - Immunology 2019Quote: ... Recombinant proteins were biotinylated with EZ-Link Sulfo NHS-LC-LC biotin (Thermo Fisher) at a 50 molar excess for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were cultured on 6-well plates coated with Vitronectin Recombinant Human Protein (Invitrogen) at a concentration of 0.5 µg/cm2 ...
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bioRxiv - Microbiology 2019Quote: ... Purified recombinant proASP1 was crosslinked to 0.5 ml of AminoLink Coupling Resin (Thermo Fisher) and affinity purified anti-ASP1 antibodies were eluted with 0.2 M Glycine ...
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bioRxiv - Microbiology 2020Quote: ... A recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant Tau proteins were biotinylated with 5 molar excess of NHS-biotin conjugates (Thermofisher) during 4 hours at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The recombinant eGFP reporter BAC was linearized using the restriction enzyme BsiWI (Thermo scientific) and purified using a CL4b sepharose column (Pharmacia) ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant baculovirus was generated using the Bac-to-Bac Baculovirus expression system (Invitrogen) and the proteins were expressed in Sf9 insect cells infected at a density of 1.0 x 106 ml−1 with P3 baculovirus stocks ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant hACE2-Fc protein was first biotinylated using EZ-Link Sulfo-NHS-Biotin (ThermoFisher) as the instruction described ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant RBD proteins were produced in transfected FreeStyle 293F cells (Invitrogen, Carlsbad, CA, USA) and purified by nickel affinity chromatography ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Thermo Fisher Scientific). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... a recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... linearized recombinant pCS2+ plasmids using the mMESSAGE mMACHINE SP6 transcription kit (Thermo Fisher Scientific).