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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for CD8 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... goat α rabbit Cy3 or donkey α rabbit Alexa488 (ThermoFisher). Neuronal membranes were visualized with 1:100 goat α HRP-Alexa 647 (Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-rabbit secondary antibody (Thermofisher anti-rabbit IgG AF633). Images were acquired using the Thunder Imager Tissue (Leica ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit-antiespin (Invitrogen PA5-55941 ...
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bioRxiv - Neuroscience 2020Quote: ... calretinculin (rabbit, ThermoFisher), SARS-CoV/SARS-CoV-2 Spike S2 (mouse ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal (Invitrogen) immunofluorescence 1/100 ...
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bioRxiv - Neuroscience 2019Quote: ... GST(rabbit, Invitrogen), GFP (rabbit ...
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bioRxiv - Neuroscience 2019Quote: ... GFP (rabbit, Invitrogen), GluN2A(rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit-Cxcr4 (Thermofisher), and rat anti-somatostatin (Millipore) ...
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bioRxiv - Cell Biology 2023Quote: α-rabbit (Invitrogen, A10042), donkey α-mouse (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: A mAb producing CHOK1 cell line (CHOK1-mAb) was seeded at a density of 2 × 105 cells/ml in SFM-II media (Gibco, 12052098) in a Kuhner orbital shaker (170rpm ...
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bioRxiv - Immunology 2020Quote: ... Reactivity of the CIS43 mAb for the CIS43 VLPs was determined by measuring optical density at 450□nm (OD450) using an AccuSkan platereader (Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... and subsequently stained for 1 h at room temperature with anti-NP mAb 1C7 antibody labeled with an Alexa488-fluorescent marker using the Alexa Fluor™ 488 Antibody Labeling Kit (Invitrogen). Immunofluorescence-labelled cells were washed twice in PBS-EDTA after incubation with the antibody and then subjected to flow-cytometry analysis using a Gallios flow cytometer (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-ELAV (MAb 7D, 1:20, which recognizes 7 amino acids unique to ELAV) and anti-GFP (1:250; Invitrogen A11122) and visualized with Alexa Fluor 488- and/or Alexa Fluor 647-coupled secondary antibodies (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... washed three times in HBSS/1% BSA and incubated at 4 °C for 15 minutes in the dark with the following antibodies: LDLR (1/100; MAB CL 472413; Fisher scientific), CD36 (1/10 ...
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bioRxiv - Immunology 2022Quote: ... Fc and non-digested mAbs were removed from the flow-through by a 2 h incubation at RT with 200 µl of protein A resin per mg of initial mAb (Thermo Scientific). Finally ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Immunology 2019Quote: ... for the HC and LC of each mAb was transfected into 5ml of suspension Expi293 cells according to the manufacturer’s instructions (Thermo Fisher Scientific) in 50ml tubes ...
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bioRxiv - Biochemistry 2020Quote: ... primary CD4+ T cells were isolated from cones from anonymous donors with approval from the National Health Service Blood and Transplant (NHSBT) and stimulated with CD3 and CD28 mAbs on Dynabeads (Thermo Fisher) in the presence of 100 U/ml IL-2 to produce T cell blasts ...
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bioRxiv - Immunology 2020Quote: ... the 5D5Δg Fab was produced by site-directed mutagenesis of the mAb 5D5 VH region using Accuprime Pfx Supermix (Thermo Fisher Scientific). 5D5Δg Fab was expressed in HEK293F cells and purified by chromatography as described above.
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Cryptococcus neoformans secretes small molecules that inhibit IL-1β inflammasome-dependent secretionbioRxiv - Immunology 2019Quote: ... cells were blocked for one h with a 10% FBS solution and stained for GXM with mAb 18B7 (10µg/mL) as a primary Ab and “Alexa Fluor® 633 Goat Anti-Mouse IgG” (Life Technologies) as a secondary Ab ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies (anti-mouse nsP2 and E2 mAbs) were then added for 1h followed by incubation with Alexa Fluor (AF) 488-conjugated chicken anti-mouse antibody (Invitrogen, USA). Approximately 1×104 cells were acquired by the LSR-Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Microbiology 2020Quote: ... Cells were immunolabelled for 1 hour at RT with the indicated murine mAb and 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Life Technologies). Immunolabelled coverslips were counterstained with DAPI (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... for 20 min before incubation overnight at 4°C in PBS-BR containing any of the following mAbs or reagents: DAPI nucleic acid (Molecular Probes), phalloidin Alexa fluor 488 (Molecular Probes) ...
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bioRxiv - Pathology 2024Quote: The binding affinity of Gremlin-1 or Gremlin-1-mAb mixtures to fluorescein-labelled Heparin (Heparin-FL, H7482, Thermo Fisher Scientific) was measured in a fluorescence polarization assay ...
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bioRxiv - Microbiology 2024Quote: ... Polprim and RPA -specific mAbs (approximately 100 µg of each ligand) were separately bound to 5mg of M-280 Tosyl activated Dynabeads (Invitrogen # 14203) O/N at 37⁰C on a rotating wheel ...
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bioRxiv - Immunology 2023Quote: The 14.4.4 mAb producing hybridoma cell line (isotype: mouse IgG2a, kappa) was maintained at 37 °C and 5% CO2 in RPMI (Life technologies) supplemented with 5% FCS (Biowest) ...
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bioRxiv - Immunology 2023Quote: ... The 14.4.4 mAb and the I-Ak-reactive 11-5.2 mAb were randomly labeled via available lysine residues with Alexa Fluor 647 carboxylic acid succinimidyl ester (Thermo Fisher Scientific) according to the manufacturer’s instructions and purified via gel filtration (Superdex-200 10/300 GL ...
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bioRxiv - Microbiology 2023Quote: Splenocytes were stimulated with the peptide pools described in the memory phenotypic assay and supplemented with anti-CD28 and anti-CD49d mAbs (ThermoFisher Scientific) both at 1.0 μg/ml or a tissue culture medium with 1% DMSO as a negative control ...
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bioRxiv - Biochemistry 2023Quote: ... The anti-F or G mAbs were prepared under serum-free conditions using HyClone SFM4MAb (Thermo Fisher Scientific Inc., Rockford, IL) and purified by protein G-Sepharose affinity chromatography.
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Immunology 2023Quote: ... T cells were distributed into the round bottom 96 well plates at 3000 cells per well and stimulated by CD3/CD28 mAb-coated beads (ThermoFisher Scientific) at a ratio of 10:1 ...
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bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Genomics 2023Quote: A mAb producing CHOK1 cell line (CHO K1 mAb) was seeded at a density of 2 × 105 cells/ml in 50ml SFM-II media (Gibco, 12052098) in 8 replicate shake flasks in a Kuhner orbital shaker at 170rpm at 5% CO2 ...
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bioRxiv - Immunology 2023Quote: Cryopreserved/thawed PBMCs were stained for 30 minutes at 4°C with the following reagents or conjugated anti-human mAbs: Live/Dead Aqua (Life Technologies), anti-CD4-PerCP (L200 ...
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bioRxiv - Microbiology 2023Quote: ... berghei infected HepG2 cells were immunolabeled using anti-PbHSP70 (1:200, 2E6 mouse mAb) (62)to mark EEFs followed by donkey anti-mouse Alexafluor488 (Invitrogen A21202). MSP1 was immunolabeled with Anti-PBANKA_083100 ...
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bioRxiv - Microbiology 2024Quote: ... plates were coated with 50 µl of capture anti-FLAMOD monoclonal antibody (Mab, 9H10-R2-2E8 mouse IgG1 - lot vt220802-7034) at 5µg/ml in D-PBS 1X (PBS, Gibco 14190-094) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and CD8 αβ T cells (gating strategy shown in Supplement to Figure 4-Figure 2B) included Fixable Viability Dye-eFluorTM780 (ThermoFisher 65-0865-14); mouse α-pig CD3ε-PE-Cy7 (BD 561477) ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were then activated with Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Scientific, Cat. #11161D) at a concentration of 25 μL/1 x 106 cells ...
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bioRxiv - Immunology 2024Quote: ... for the last 4h of culture and stained with anti-CD4 (PerCP-Cy5.5) anti-CD8 (APC) monoclonal Ab (Thermo Fisher Scientific, Waltham, MA, USA). Cells were fixed ...
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bioRxiv - Microbiology 2020Quote: ... All PCR reactions were carried out using Recombinant Taq DNA Polymerase (5U/μl from Thermo Scientific) and standard PCR cycling conditions.
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bioRxiv - Cell Biology 2022Quote: ... Purified recombinant plasmid DNA was digested with PacI and transfected into HEK293 cells with Lipofectamine (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Recombinant antibodies were transiently transfected and produced in vitro in 293F Freestyle suspension cells (Thermofisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... and recombinant expression was undertaken using the FreeStyle™ MAX 293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2019Quote: ... iPSC line WTC-11 (Coriell Institute) was maintained on recombinant human vitronectin[36,37](Thermo Fisher Scientific), coating six-well tissue culture plastic plates (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... The positive control consisted of 100 – 500 ng recombinant Protein A (ThermoFisher Scientific, Santa Clara, CA). The negative control consisted of ~2 μg wt-TMV from purified N ...
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bioRxiv - Biochemistry 2021Quote: ... the recombinant GST-SHP2 in supernatant was affinity-purified by Pierce glutathione agarose (Thermo Fisher Scientific) and eluted with lysis buffer containing 20 mM GSH ...
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bioRxiv - Zoology 2021Quote: ... The recombinant plasmid was quantified using a NANO-DROP 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). The plasmid copy number was calculated using the equations described by Yun et ...