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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was slowly thawed at 4°C and treated with 10 U of Turbo DNase (Thermo Fisher Scientific) on ice for 10 min to remove the genome DNA ...
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bioRxiv - Microbiology 2023Quote: ... and loaded 10 µl (100 ng) of the sample on NuPAGE 4–12% Bis-Tris Protein Gel (Thermo Fisher), separating proteins by electrophoresis with Chameleon Pre-Stained Protein Ladder (Li-Cor ...
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bioRxiv - Neuroscience 2023Quote: ... 10 μg of protein from extracts were separated in NuPAGE 4-12% Bis-Tris Gel 1.0mm (NP0321BOX, Thermo Scientific) or 3-8% Tri-Acetate gel (EA03755BOX ...
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bioRxiv - Systems Biology 2023Quote: ... then ran 20 µg total protein on a NuPAGE 4-10% BisTris Gel at 240V for 40 minutes (ThermoFisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... Samples were denatured for 2 minutes at 90°C and hemolymph from 10 mosquitoes was loaded on each lane of a 4 - 20% 1.5mm polyacrylamide gel (Invitrogen). After electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 °C) for 10 minutes and the aqueous layer was transferred and precipitated with isopropanol and glycoblue (Thermo Fisher) at room temperature for a few minutes ...
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bioRxiv - Pathology 2023Quote: ... fixed in 4% paraformaldehyde for 10 min and stained with ALP staining kit (Thermo Fisher Scientific, Rockford, IL, USA) for 15 min.
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bioRxiv - Biochemistry 2023Quote: ... Handcast SDS-PAGE gels (8%, 10%, 15%, or 4-18% gradient) containing 0.5% (v/v) trichloroethanol (Acros Organics 139441000) for stain-free imaging (79 ...
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bioRxiv - Bioengineering 2024Quote: ... Protein (10 μg) from each sample was combined with 1X Bolt LDS Sample Buffer (1:4, Invitrogen catalog #B0007) and 1X Bolt Reducing Agent (1:10 ...
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bioRxiv - Molecular Biology 2024Quote: ... Isolated pericentral/periportal hepatocytes were rocked for 10 min at 4 °C with RIPA lysis buffer (ThermoFisher Scientific, #89900) with protease and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... A final concentration of 10 μM Cell Tracker Blue CMAC (7-amino-4-chloromethylcoumarin) dye (Life Technologies; Carlsbad, CA) and 1 μM of MG-TAU dye (cell permeant dye αRed-np1 ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 µg protein were loaded onto 4-12% Bis-Tris polyacrylamide gel (Thermo Fisher Scientific; Cat No. NP0336BOX) Nitrocellulose (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: 30 μL of protein extract were subjected to SDS-PAGE on a 10-well NuPAGE 4–12% gradient (Invitrogen) for 6 min at 200 V with MES buffer ...
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bioRxiv - Microbiology 2024Quote: ... Samples were centrifuged at 13,000 rpm for 10 min at 4°C and further processed using the RiboPure RNA Purification Kit (#AM1924, Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: 10 µg of protein from the bead eluent were loaded on 4 to 12% gradient Bis-Tris gels (ThermoFisher) and run until loading dye reached bottom of gel ...
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bioRxiv - Microbiology 2024Quote: ... mucus lysates (10 µg of proteins) were loaded onto a Bolt 4–12% Bis-Tris gradient gel (#NW04120BOX, Invitrogen). Proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: ... fluorescent antibodies (4 µg/mouse) and dyes (2 µl of a 10 mM Sytox Green solution; Thermo Fisher Scientific) were dissolved in 100 µl sterile PBS and injected intravenously 10 minutes before the surgery ...
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bioRxiv - Microbiology 2024Quote: ... Microorganisms were visualised using the membrane stain FM 4-64 (10 µg/ml in 1 x PBS; T13320, Invitrogen). DNase I (04716728001 ...
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bioRxiv - Cell Biology 2024Quote: ... on 4 ng of cDNA in a 10 µL reaction consisting of SYBR green master mix (Applied Biosystems #4367659) with 0.3 µM forward and reverse primers (Table 1).
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bioRxiv - Plant Biology 2024Quote: ... We loaded 10-20μL of proteins into 4–12% for total protein or 12% acrylamide gels for histone extracts (Invitrogen). We transferred the proteins from the gel to an Immobilon-P membrane (Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... at 99°C for 10 min and proteins were separated on NuPAGE 4–12% Bis-Tris Gels (ThermoFisher Scientific). They were then transferred onto a PVDF membrane using the iBlot system (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... then fixed for 10 minutes at room temperature with a 4% Paraformaldehyde-PBS solution (Thermo Fisher #28906; Gibco #10010023). Cells were washed 3x with PBS ...
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bioRxiv - Bioengineering 2024Quote: ... then fixed for 10 minutes at room temperature with a 4% Paraformaldehyde-PBS solution (Thermo Fisher #28906; Gibco #10010023). Cells were washed 3x with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10 μg total protein was run on Novex™ WedgeWell™ 4-20% Tris-Glycine Mini Gels (Invitrogen) and transferred to an Immobilon-P membrane (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... plus 10% glycerol and 0.5mM dithiothreitol) at 4°C or with the addition of 20μg/ml RNase A (Thermofisher Scientific). Following washing of antibody-bead complexes five times in Co-IP lysis buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The fluorescent carboxylate modified microspheres (fluorescent latex beads) (Thermo Fisher Scientific, Catalog # F8813) were a kind gift from Prof ...
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bioRxiv - Biophysics 2021Quote: ... 20 nm or 200 nm carboxylate-modified fluorescent beads (FluoSpheres, Invitrogen, ThermoFisher, USA) were diluted in calcium-free fibrin buffer ...
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bioRxiv - Biophysics 2021Quote: ... 20 nm or 200 nm carboxylate-modified fluorescent beads (FluoSpheres, Invitrogen, ThermoFisher, USA) were diluted in calcium-free fibrin buffer ...
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bioRxiv - Biophysics 2022Quote: ... and 15 μl 2% w/v 0.5 μm carboxylate fluorescent beads (Thermo Fisher) were added to 1 ml DI water ...
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bioRxiv - Bioengineering 2022Quote: ... and 1:20,000 dilution of red fluorescent 1μm carboxylate-modified microspheres (Invitrogen, USA) was prepared ...
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bioRxiv - Cell Biology 2022Quote: ... TEMED and FluoSpheres® carboxylate-modified microspheres (diameter 0.2 μm, Molecular Probes-F8801) was added to the dishes and covered by a pre-coated with 1mg/ml fibronectin 13mm coverslip ...
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bioRxiv - Immunology 2022Quote: ... TransFluoSpheres™ Carboxylate-Modified Microspheres (1.0 μm diameter, 488nm/560nm excitation/emission, ThermoFisher) were coupled directly with 2019-nCoV trimeric Spike protein or with streptavidin according to the manufactures protocol ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µL of 0.2 µm red beads FluoSpheres carboxylate-modified (580/605, Invitrogen) were added to the PAA gel solution ...
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bioRxiv - Cell Biology 2024Quote: ... The fiducial solution also included 500 nm carboxylate modified red fluospheres (Invitrogen F8812) for grids #5 (50 µg/mL) ...
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bioRxiv - Neuroscience 2024Quote: ... and coated with 200 nm fluorescent beads (FluoSpheres Carboxylate-Modified Microspheres, F8811, Invitrogen). After that ...
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bioRxiv - Genetics 2021Quote: ... and cytocentrifuged on clean slides (using a Cytospin™ 4 Cytocentrifuge, Thermo Fisher Scientific, at 900 rpm for 4 min). Slides were then immersed in liquid nitrogen for 5 min ...
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bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then nutated overnight at 4 °C with 1 ml of Protein G Sepharose 4 Fast Flow slurry (Invitrogen) per 25ml of supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating coronal hippocampal slices of 50 µm thickness were produced and incubated overnight in staining solution (4% normal goat serum, 0.4% Triton-X 100 and 4% BSA) with anti-GFAP antibody (1:1000, ThermoFisher, rat) and anti-Iba1 antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Handpicked islets (~180) from control (n=4) and diabetic (n=4) mice were placed into 1 mL Trizol (Cat# 15596018, Ambion) and processed for RNA extraction ...
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bioRxiv - Microbiology 2021Quote: ... for 20 min at 4 °C and were then centrifuged at 444g for 4 min and dissociated to single cells using StemPro Accutase (Invitrogen). Cells were subsequently fixed using 4% paraformaldehyde (PFA ...
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bioRxiv - Bioengineering 2020Quote: ... and media for M(IL4/IL13) stimulation consisted of RPMI 1640 with 20 ng/ml interleukin 4 (IL-4; Invitrogen) and 20 ng/ml interleukin 13 (IL-13 ...
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bioRxiv - Biochemistry 2021Quote: ... 6,8-Difluoro-4-Methylumbelliferyl Phosphate (DiFMUP, #D22065) and 6,8-difluoro-7-hydroxy-4-methylcoumarin (DiFMU, #D6566) were purchased from Life Technologies.
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bioRxiv - Biochemistry 2020Quote: ... The isolated fractions were separated on SDS-PAGE (RunBlue 4-20 %, Expedeon; NuPAGE®Bis-Tris gel 4-12%, Invitrogen) and analysed by InstantBlue staining (Expedeon ...
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bioRxiv - Biochemistry 2021Quote: ... gondii parasites were separated by polyacrylamide gel electrophoresis (PAGE) in precast NuPAGE (4-12% or 12%) or NativePAGE (4-16%) gels (ThermoFisher) according to the manufacturer’s instructions with minor modifications (detailed in the SI) ...
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bioRxiv - Microbiology 2021Quote: ... loaded equivalently in each lane (ranging from 4-20 μg between experiments) and run on a 4-20% Tris-Glycine gel (Novex, Invitrogen) under reducing conditions ...
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bioRxiv - Microbiology 2021Quote: ... Streptomyces hyphae were incubated with 0.5 mg/ml FM 4-64 Dye (N-(3-Triethylammoniumpropyl)24-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Molecular Probes) for 15 min in the dark ...
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bioRxiv - Biophysics 2020Quote: ... The samples were incubated for 20 min at 4°C before they were loaded onto a pre-run 4-20% Novex TBE gel (Invitrogen). Electrophoresis was performed at 200 V for 45 min in TBE buffer (89 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (KCL-22, K562, REH, NB-4) or 20% (MOLT-4, OCI-AML3, MOLM-13, Kasumi-1) fetal bovine serum (Gibco) and 1% penicillin–streptomycin (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 4 μg of a plasmid mixture (1:1:4 THR:RXR:TRE-Luc) in 200 μL Opti-MEM (Invitrogen, 11058-021) and added to the cells ...