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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and resuspended in fresh DMEM-10 (Gibco DMEM #11995-065 with 4.5 g/L D-Glucose and 110 mg/L Sodium Pyruvate, 10% Fetal Bovine Serum [HyClone], 1% HEPES [Gibco 1M 15630-080] ...
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bioRxiv - Biophysics 2022Quote: ... and Caco2 cells were cultured in 4.5 gl−1 DMEM containing 10% FBS (20% for Caco2) and 1% Penicillin-Streptavidin (Gibco, Thermo Fischer Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and MDA-MB-231 cells were maintained in 10% CO2 at 37ᵒC with high-glucose (4.5 g/L) Dulbecco’s modified Eagles Medium (DMEM; Thermo Fisher, #11965092) supplemented with 10% HyClone fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... Crosslinks were reversed by incubating the bound complexes in 60 uL TE containing 4.5 uL of 10% SDS and 7.5 uL of 20 mg/mL of proteinase K (Thermofisher, USA) at 65°C overnight for input samples ...
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bioRxiv - Cell Biology 2020Quote: ... Caco2 and MDCK cells were routinely grown in DMEM 4.5 g/l glucose supplemented with 20% (Caco2 cells) or 10% fetal bovine serum and 1% penicillin-streptomycin (Gibco, Thermo Fischer Scientific ...
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bioRxiv - Immunology 2022Quote: ... using 4.5 mL to transform 8 to 10 million PBMCs in B cell growth medium (Medium A containing CpG [Invitrogen, oligo ZOEZOEZZZZZOEEZOEZZZT] at the 10 μmole scale [desalted] ...
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bioRxiv - Neuroscience 2023Quote: ... The digest was mechanically disrupted with a serological pipette in complete media (DMEM [Gibco; 11965-092] 4.5 g/dL glucose, 10% FBS [Gibco], 50 U/mL penicillin, 50 µg/mL streptomycin [Gibco]). Cell suspension was filtered through a 70 µm cell strainer ...
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bioRxiv - Neuroscience 2023Quote: ... The digest was mechanically disrupted with a serological pipette in complete media (DMEM [Gibco; 11965-092] 4.5 g/dL glucose, 10% FBS [Gibco] ...
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bioRxiv - Immunology 2021Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
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bioRxiv - Cancer Biology 2022Quote: ... HepG2 cells were fixed with 4% paraformaldehyde and then stored at 4°C in RPMI 1640 (Gibco, Cat# 11875119) media supplemented with 10% heat inactivated FBS ...
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bioRxiv - Bioengineering 2021Quote: ... Stock BODIPY505/515 was prepared by dissolving 4,-Difluoro-1,3,5,7-Tetramethyl-4-Bora-3a,4a-Diaza-s-Indacene (Life Technologies, D3921) powder into dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed using a 4% PFA solution [0.2M sucrose | 4% paraformaldehyde solution in PBS (Thermo Fisher, J19943-K2)] ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates and virions pelleted through 20% sucrose (14,000xg for 90 minutes at 4°C) were separated on a NuPage 4-12% Bis-Tris Gel (Invitrogen) and subsequently blotted onto a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were then applied at 4°C overnight in 1x PBS supplemented with Horse serum (4%, ThermoFisher, 16050114). The following synaptic antibodies were used ...
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bioRxiv - Developmental Biology 2022Quote: ... and passaged very 3-4 days with a 1:4-6 passage ratio using Versene solution (Life Technologies 15040066).
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bioRxiv - Synthetic Biology 2020Quote: ... 4 µl CFE reaction was loaded onto a NuPAGE 4–12% Bis–Tris Gel (Life Technologies, Carlsbad, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Cells were plated at a density of 4 × 104 cells per well in a 4 well chamber slide (Nunc Lab-Tek II Chamber slide system ...
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bioRxiv - Immunology 2021Quote: Avi-tag-biotinylated spike and RBD were incubated with subsequent fluorochrome-labeled streptavidin at 4:1.5 ratio overnight at 4 °C as follows: spike with APC-streptavidin (Invitrogen), Wuhan RBD with PerCP-Cy5.5-streptavidin (BioLegend) ...
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bioRxiv - Molecular Biology 2021Quote: Protein samples (4×106 cell equivalents per lane) were separated on 4%-12% Bis-Tris gels (Thermo Fisher Scientific) and then transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2022Quote: ... or BA.4/5 spike protein (4 μg/mL) was immobilized on 96-well Maxisorp ELISA plates (Thermo Fisher) overnight at 4°C in coating buffer (1X PBS supplemented with 0.05% Tween-20 ...
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bioRxiv - Plant Biology 2024Quote: ... for 6h at 4°C and an additional 2h at 4°C with 20μl Dynabeads Protein G (#10003D, ThermoFisher) blocked with 0.1% BSA ...
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bioRxiv - Biophysics 2024Quote: ... 4 µl of cell suspension were applied and plunge-frozen using a Vitrobot Mark 4 system (Thermo Fisher Scientific) at blot force 10 ...
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bioRxiv - Biophysics 2023Quote: ... The protein was incubated overnight at 4°C with stirring with 4× molar excess AL594 or AL647 maleimide (Invitrogen). The labeled protein was concentrated and buffer exchanged into 20 mM Tris pH 7.4 ...
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bioRxiv - Bioengineering 2023Quote: B-RBD cells (1 × 107 cells/mL) were combined with 4 μM Fluo-4 AM (Invitrogen, USA, Cat: #F14217) for 20 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... were treated either with 0.8 μM CF-4 or control CF-4 (Ctrl-S2-CF4) in DMEM without phenol red (Gibco) and incubated for 10 min at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8) was purchased from ThermoFisher Scientific.
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bioRxiv - Bioengineering 2023Quote: ... HA-tetrazine was synthesized as described previously using 79 kDa HA with molar equivalents of 1:1:0.25 of HA-repeat units to 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) (Thermo Fisher Scientific) to tetrazine-amine (Chem-Impex).[16] 1H NMR shifts of pendant tetrazine groups at δ8.5 (2H ...
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bioRxiv - Immunology 2023Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were spun down at 1,000xg for 4 min and fixed with 4% PFA (Thermo Fisher, Cat. No. 28908) for 15 min on ice ...
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bioRxiv - Bioengineering 2024Quote: Live and dead cells were stained with 1.6-4 mM calcein AM (C125400; TRC) and 4 mM ethidium homodimer-1 (EthD-1 L3224; ThermoFisher), respectively ...
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bioRxiv - Immunology 2024Quote: ... The cells were cultured for 2 weeks and then washed and fixed in 4% paraformaldehyde (4% PFA; Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... on a rotating mixer for 4 h at 4°C while 300 uL protein G magnetic beads (Dynabeads; Invitrogen) were washed on a magnetic stand (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed three times with 10 mL prewarmed commercial DPBS and harvested with 10 mL prewarmed 4 mM EDTA (Thermo Fisher Scientific, J15694.AE) diluted in commercial DPBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Pelleted cell mixtures were resuspended in 10 μl of 1:9 collagen/setting buffer (10× Earle’s Balanced Salt Solution (Life Technologies), 0.2 M NaHCO3 and 50 mM NaOH) ...
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bioRxiv - Biochemistry 2022Quote: ... and loaded onto a 10% gelatin gel (9-10 µL for apical, 18-20 µL for basal, Novex, Life Technologies). Samples were electrophoresed for ∼90 min at 125-140V ...
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bioRxiv - Cell Biology 2023Quote: ... HEK and N/TERT-2G keratinocytes were cultured in EpiLife media (Life Technologies) supplemented with Human Keratinocyte Growth Supplement (HKGS S0015 ...
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bioRxiv - Molecular Biology 2024Quote: ... and IMR-90 tert (human lung fibroblast) cells were cultured in DMEM (Gibco), supplemented with 10% (v/v ...
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bioRxiv - Biophysics 2022Quote: ... the aqueous solution also included a 10−5 dilution of 40 nm fluorescence nanoparticles (FluoSpheres carboxylate-modified microspheres, red-orange fluorescence, ThermoFisher Scientific, Waltham, MA, USA), and the solution was sonicated for 15 minutes before each use ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed three times and stained with 10 µM SYTO 9 (Invitrogen, USA) in PBS for 10 minutes ...
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bioRxiv - Genetics 2022Quote: ... 9 and 10 levels were analyzed using a TSQ Altis triple quadrupole mass spectrometer (ThermoFisher) coupled to a Vanquish LC system (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... cells previously seeded overnight were incubated for 4 h with 10 μM Alexa Fluor™ 594-dextran (Invitrogen, D22913) and analysed in live by laser confocal microscopy.
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bioRxiv - Genetics 2021Quote: ... 10 μg of protein were loaded onto a 15-well NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen), run at 100 V for 2.5 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Cell transfections were carried out in 96-well plate with 1.0×10^4 target cells where each well received 100 ng DNA with a mix of Lipofectamine 3000 (Invitrogen) in Opti-MEM.
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The tube was then centrifuged at 1600 rpm for 10 minutes at 4°C and the pellet resuspended in Hank’s Buffered Saline Solution (#14175095, Gibco, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... for 60 minutes at 4 °C and the reaction was stopped with 10 μl SuperaseIn (Thermo Fisher Scientific AM2696). Ribosome-protected fragments were isolated using microspin S-400 (GE HealthCare ...
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bioRxiv - Microbiology 2021Quote: ... Harvested cells were normalized to the lowest OD-600 of the collected cells and pelleted by centrifugation at 20000XG for 2 mins and resuspended in 75 µL lysis buffer (10 mM Tris-HCl, pH 7.0, 4% SDS, 5 U DNaseI (ThermoFisher), 0.2 tablets of PhosStop (Roche)) ...
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bioRxiv - Bioengineering 2021Quote: ... Bacteria were washed and pelleted with PBS via centrifugation (13,180 × g at 4 °C for 10 min) with a superspeed centrifuge (ThermoFisher). Afterwards ...
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bioRxiv - Neuroscience 2020Quote: ... at 95 °C for 5-10 min and loaded on a gradient 4-12% NuPAGE Bis-Tris gels (Invitrogen). After electrophoresis at 200 V ...
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bioRxiv - Cell Biology 2021Quote: ... transfected with respective siRNAs (200 pmol/ well) on day 2 and day 4 using oligofectamine (Invitrogen; 10 μl/ well), and processed on day 6 for IFM or biochemical analyses ...
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bioRxiv - Bioengineering 2021Quote: ... and was fixed in 10% neutral-buffered formalin (NBF) at 4 °C for 72 h and stored in PBS (pH 7.4; Gibco) at 4 °C for subsequent NMR spectroscopy and imaging experiments.