Labshake search
Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... and equivalent amounts of protein were mixed with 4× LDS sample buffer and 10× reducing agent (Thermo-Fisher Scientific), followed by denaturation at 70 °C for 10 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Insoluble proteins were pelleted by centrifugation (15,000 x g for 10 min at 4°C) and supernatants were analysed using a UHPLC system (Vanquish, ThermoFisher) coupled online to a mass spectrometer (Q Exactive ...
-
bioRxiv - Cell Biology 2021Quote: ... followed by electroporation using the Neon electroporation system with a 10-µl tip according to the manufacturer’s instruction (Pulse voltage: 1200 V, pulse width: 25, pulse number: 4, Invitrogen). The final concentrations of the annealed crRNA/tracrRNA ...
-
bioRxiv - Bioengineering 2022Quote: ... lungs were rinsed with antibiotics/antimycotics (10% P/S, 4% amphotericin-B [Sigma], 0.4% gentamicin [Gemini Bio] in PBS with Ca2+ and Mg2+ [Gibco]) followed by perfusion with 0.0035% Triton X-100 (American Bioanalytical) ...
-
bioRxiv - Cell Biology 2022Quote: ... Remnant villi were removed by shaking tissue pieces at 4°C in an initial 5 minute incubation PBS with 10 mM RNase-free EDTA pH 8.0 (Invitrogen). Crypts were collected and filtered through 70 µm cell strainers in subsequent fractions ...
-
bioRxiv - Microbiology 2022Quote: ... incubated for 10 min in the dark at room temperature with FM 4-64FX (3 μg ml-1) (ThermoFisher). Cells were applied to a 5% agarose pad containing M9 minimal media with glucose (0.4%) ...
-
bioRxiv - Developmental Biology 2020Quote: ... 10% dialyzed fetal bovine serum (PAA),4 mM L-glutamine (PAA),1% penicillin/streptomycin (100 IU/100 μg, Invitrogen). For Light Medium ...
-
bioRxiv - Molecular Biology 2020Quote: ... Chromatin was clarified by centrifugation at 21,000 g at 4°C for 10 min and precleared with 80 µl prewashed Dynabeads protein A (ThermoFisher) for 30 min at 4°C ...
-
bioRxiv - Genetics 2021Quote: ... for 10 minutes at 70°C before loading into Bolt 4-12% Bis-Tris Plus gels (Thermo Fisher Scientific). Gels were run at 120V for 2-3 hours in Bolt MES SDS Running Buffer (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... 10 μg of protein from extracts were separated in SDS–polyacrylamide gels 4–12% (NuPAGE Bis-Tris, Thermo Scientific) and transferred to nitrocellulose membranes (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were marked with 2,3×10−3 μg/μL 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Thermo Fisher Scientific) for 10 minutes at room temperature in the dark ...
-
bioRxiv - Molecular Biology 2022Quote: ... 10 μl of proteins obtained after TCA precipitation were separated by 4-12% polyacrylamide gradient gel electrophoresis (NuPAGE-Invitrogen) in 1X MES buffer (50 mM MES ...
-
Mutation in the matricellular gene fibulin-4 leads to endothelial dysfunction in resistance arteriesbioRxiv - Physiology 2022Quote: ... Vessels were then fixed in 4% paraformaldehyde for 10 min and buffer containing DAPI (1:2,000, Thermofisher, Waltham, MA) for 5 min ...
-
bioRxiv - Cell Biology 2020Quote: ... Ni-NTA His.Bind® Resin (2-4 ml) was packed into a 10 ml Pierce disposable column (Thermo Fisher) and connected to the peristaltic pump ...
-
bioRxiv - Microbiology 2022Quote: ... at 90°C for 10 min before being run on a 4-12% acrylamide denaturing gel (ThermoFisher, #NP0323 NuPAGE). Proteins were then transferred onto a nitrocellulose membrane (ThermoFisher ...
-
bioRxiv - Genetics 2022Quote: ... centrifuged for 5 minutes at 500xg at 4°C and resuspended in 70µl flow cytometry buffer (PBS, 10% ESC-grade FBS (Gibco), 0.5mM EDTA ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Homogenised leaf tissues were centrifuged at 18,000 g 10 min 4 °C and 2 μL supernatant mixed with 48 μL Bradford reagent (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... For PhoX crosslinking we used a Fe3+-IMAC column (Propac IMAC-10 4 × 50 mm column, Thermo Fisher scientific) connected to an Agilent HPLC ...
-
bioRxiv - Molecular Biology 2021Quote: ... Samples were washed 4 x 10 minutes in PBS-Tr at room temperature with DAPI (1:5,000, Invitrogen D1306) added during the third wash ...
-
bioRxiv - Genomics 2020Quote: ... The chromatin was then cleared by centrifugation (12,000 g for 10 minutes at 4°C) and 80 μL of protein G DynaBeads (ThermoFisher, washed 3 times in cold ChIP buffer right before use ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 10-100 μg total protein was then separated with a 4-12% (v/v) gradient Bis-Tris gel (ThermoFisher) run in MES buffer system ...
-
bioRxiv - Cancer Biology 2022Quote: ... Lysates were cleared of insoluble material by centrifuging at 20,000 g for 10 min at 4°C and protein concentration was determined with the BCA Protein Assay (ThermoFisher). For immunoblotting 20-40 μg of protein in equal volumes were heated in LDS-sample buffer (Invitrogen ...
-
bioRxiv - Neuroscience 2020Quote: ... 10 μg of protein was loaded into a 15-well NuPAGE 4-12 % Bis-Tris gel (Cat# NP0336BOX, ThermoFisher) and run in MES buffer (50 mM MES ...
-
bioRxiv - Microbiology 2022Quote: ... heated for 10 min at 95°C and separated on 4–12% Bis-Tris NuPAGE gels (Thermo Fisher Scientific) in the presence of antioxidant (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... The samples were centrifuged at 13,300 rpm for 10 minutes and ran on NuPage 4-12% Bis-Tris polyacrylamide gels (Invitrogen). Proteins were transferred onto a Immobilon PVDF membrane (Millipore) ...
-
bioRxiv - Immunology 2023Quote: ... Cells were pelleted by centrifugation at 500xg for 10 minutes at 4°C and re-suspended in 1ml of 0.2µM SYTOX Green (Invitrogen; S7020) reagent diluted 1:1000 in PBS from a 5mM stock ...
-
Functional characterization of ATP13A2 variants associated with distinct neurodegenerative disordersbioRxiv - Molecular Biology 2023Quote: ... 10 μg of protein was loaded on a NuPage 4-12% Bis-Tris gel (Thermo Fisher Scientific, Bio-Rad) and separated during an electrophoresis run at 150 V in MES running buffer (Thermo Fisher Scientific) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The lysate was slowly thawed at 4°C and treated with 10 U of Turbo DNase (Thermo Fisher Scientific) on ice for 10 min to remove the genome DNA ...
-
bioRxiv - Neuroscience 2023Quote: ... fixed in 4% PFA for 10 minutes at room temperature and incubated in 1 μg/ml DAPI (Life Technologies) for 10 minutes at room temperature in DPBS+/+ ...
-
bioRxiv - Microbiology 2023Quote: ... and loaded 10 µl (100 ng) of the sample on NuPAGE 4–12% Bis-Tris Protein Gel (Thermo Fisher), separating proteins by electrophoresis with Chameleon Pre-Stained Protein Ladder (Li-Cor ...
-
bioRxiv - Genetics 2023Quote: ... Samples were denatured for 2 minutes at 90°C and hemolymph from 10 mosquitoes was loaded on each lane of a 4 - 20% 1.5mm polyacrylamide gel (Invitrogen). After electrophoresis ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein concentrations of cell lysates were measured after centrifugation at 20,817g for 10 minutes at 4 °C using a Micro BCA Protein Assay Kit (Invitrogen). 50 μg of total proteins were analyzed by SDS-PAGE and Western blotting ...
-
bioRxiv - Bioengineering 2022Quote: Equal concentrations of protein were loaded in all lanes of a 10% polyacrylamide gel with a 4% stacking gels were used and prepared per manufacturer’s instructions (Invitrogen). Pre-made ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were centrifuged at 1,000g at 4 ℃ for 10 mins and supernatants were then pre-cleared with 15 μl Dynabeads Protein G (Invitrogen) for 30 mins ...
-
bioRxiv - Cell Biology 2022Quote: ... samples were separated on 10% or 4-20% gradient Novex™ WedgeWell™ Tris-Glycine gels (Thermo Fisher Scientific) for 40 minutes at 225V and transferred at 40V overnight at 4°C onto nitrocellulose membranes ...
-
bioRxiv - Biochemistry 2024Quote: Reduced protein extracts were separated on 10% SDS–PAGE gels or 4–12% NuPAGE bis–tris precast gels (Invitrogen) by electrophoresis ...
-
bioRxiv - Cancer Biology 2024Quote: ... Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher) at 37 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... 10 µL of each sample was loaded into a well of NativePAGE 4-12% Bis-Tris Gels (Invitrogen NP0321BOX). The gels were run at 150 V for approximately two hours at 4 °C and transferred onto 0.2 µm PVDF membranes (Millipore IPVH00010 ...
-
bioRxiv - Cell Biology 2024Quote: ... while 10 μg/lane of lysates were electrophoresed on Bolt 4–12% Bis-Tris Plus Gels (Thermo Fisher Scientific) for β-actin ...
-
bioRxiv - Developmental Biology 2024Quote: ... Chromatin was released from crosslinked nuclei by dissolving 300 μl of SDS buffer (50 mM HEPES pH 7.4, 10 mM EDTA pH 8.0, 4% UltraPure™ SDS Solution from Invitrogen, along with a newly added 1X cOmplete™ EDTA-free Protease Inhibitor Cocktail ...
-
bioRxiv - Microbiology 2024Quote: ... and 2 µL RNase A/T1 Mix (4 µg RNase A, 10 U RNase T1, ThermoFisher Scientific, MA, USA) at 37°C for 30 min to remove host-originating nucleic acids ...
-
bioRxiv - Cancer Biology 2024Quote: ... 10–15 µL of protein lysis were run per lane of 4–12% Bis-Tris gels (Thermo Fisher Scientific) using MOPS buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 μg of samples were loaded on SDS-PAGE gel (Novex 4%–20% Tris-Glycine Mini Gels, Thermo Scientific). Protein was transferred to a PVDF membrane 0.2 μm (Amersham ...
-
bioRxiv - Cell Biology 2024Quote: ... 10 µg protein sample was loaded in each well and ran on a 4%–12% SDS-PAGE gel (Invitrogen), then transferred to the nitrocellulose membrane (0.2 µm ...
-
bioRxiv - Systems Biology 2023Quote: ... then ran 20 µg total protein on a NuPAGE 4-10% BisTris Gel at 240V for 40 minutes (ThermoFisher Scientific ...
-
bioRxiv - Pathology 2023Quote: ... fixed in 4% paraformaldehyde for 10 min and stained with ALP staining kit (Thermo Fisher Scientific, Rockford, IL, USA) for 15 min.
-
bioRxiv - Biochemistry 2023Quote: ... Handcast SDS-PAGE gels (8%, 10%, 15%, or 4-18% gradient) containing 0.5% (v/v) trichloroethanol (Acros Organics 139441000) for stain-free imaging (79 ...
-
bioRxiv - Neuroscience 2024Quote: ... 10 μg of total protein per sample was loaded onto 4 to 12% bis-tris SDS- polyacrylamide gel (Invitrogen). Following electrophoresis ...
-
bioRxiv - Molecular Biology 2023Quote: ... 4 °C) for 10 minutes and the aqueous layer was transferred and precipitated with isopropanol and glycoblue (Thermo Fisher) at room temperature for a few minutes ...
-
bioRxiv - Immunology 2023Quote: ... Raji B cells were labelled in serum-free medium with 10 μM 7-amino-4-chloromethylcoumarin (CMAC, Thermofisher, USA) for 1 hour at 37°C ...