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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Recombinant Sheep IL12B Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... REX1 antibody DA136 (3rd bleed) was diluted at 1:1000 and visualized using Donkey anti-Sheep HRP secondary antibody (Thermo Fisher Scientific). Immunoblots were imaged using enhanced chemiluminescence on a BioRad ChemiDoc imager (BioRad) ...
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bioRxiv - Microbiology 2019Quote: ... Strains were routinely grown at 37°C and 5% CO2 overnight on tryptic soy agar plates supplemented with 5% sheep blood (TSAII) (Thermo Fisher Scientific). Growth in broth culture was performed in BHI (Becton ...
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bioRxiv - Cancer Biology 2021Quote: ... The FAP primary antibody was detected with a secondary biotin-conjugated anti-goat/sheep mouse IgG and 1:1000 Streptavidin PE-Cy7 (Thermo Fisher Scientific). EdU was detected using the Click-IT Plus Flow Cytometry Assay with AlexaFluor® 488 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... brain sections were incubated in corresponding secondary antibodies (Alexa Fluor 488 Donkey anti-rabbit IgG 1:400, Life Technologies A-21206; Alexa Fluor 555 Donkey anti-sheep IgG 1:400, Life Technologies A-21099 ...
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Elimination of glutamatergic transmission from Hb9 interneurons does not impact treadmill locomotionbioRxiv - Neuroscience 2021Quote: ... Secondary Abs (Alexa 546 Donkey anti-Rabbit; Alexa 488 Donkey anti-goat, anti-mouse, or anti-sheep, all 1:400, Invitrogen, Oregon, USA) were diluted in a solution containing 1% donkey serum and PBST ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were washed three times for 5 minutes in 0.05% PBS-Tween then incubated with appropriate secondary antibodies in the dark at room temperature (anti-IgG rabbit A488 and A647, anti-IgG mouse A555 and anti-IgG sheep A555, all from Thermo Fisher Scientific) and 1:1000 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by a donkey anti-sheep IgG coupled to horse radish peroxidase (R&D, MN) and developed with an ECL Western blotting kit (Thermo Scientific, NJ). The intensities of the cytochrome c bands were normalized to that of constitutively expressed β-actin and expressed as relative intensity ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used for immunocytochemistry were donkey anti-mouse/rabbit/goat/sheep conjugated to Alexa Fluor 488/546/555/647 (Invitrogen 1:500), respectively ...
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bioRxiv - Microbiology 2023Quote: For routine cultivation, all Streptococci were grown for 24 – 48 hours on Columbia agar (Oxoid, UK) with 5% defibrinated sheep blood (Thermo Scientific, USA) at 37°C in presence of 5% CO2 and 95 % relative humidity or in a vinyl anaerobic chamber with < 5 ppm O2 (Coy labs ...
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bioRxiv - Microbiology 2024Quote: ... and also cultured16 the fluid by spread-plating 20 microliters onto a pre-reduced tryptic soy 5% defibrinated sheep blood agar (80% N–10% H– 10% CO2, at 37°C; Thermo Fisher Scientific) using a variable-atmosphere anaerobic Whitley workstation A85 (540 plate capacity ...
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bioRxiv - Cancer Biology 2023Quote: ... goat anti-mouse or donkey anti-sheep IgG secondaries linked to Alexa Fluor 488 (1:500, Thermo Fisher #A-11034, #A-11001), Alexa Fluor 546 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... together at 4°C for 18 hours followed by appropriate secondary antibodies (donkey anti-mouse IgG [H+L]; A48257 and donkey anti-sheep IgG [H+L]; A11016; 1:1000; Invitrogen, Waltham, MA) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... followed by secondary antibody for 30 minutes (either Alexa-Fluor 488 or 594 conjugated goat anti-mouse, goat anti-rabbit, or goat anti-sheep, all at 1:200, Thermo Fisher Scientific). After nuclear staining with DAPI (4’,6-diamidino-2-phenylindole) ...
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bioRxiv - Microbiology 2023Quote: ... generated as described in [16]) was used at 1:200 (Table 1) before staining with Donkey anti-Sheep AF488 Secondary Antibody (Invitrogen, A-11015) at 1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated over-night at +4°C with donkey anti-sheep secondary antibody conjugated with Alexa Fluor 568 (1:1000, A-11011, Invitrogen, ThermoFisher Scientific) and Streptavidin conjugated with Alexa Fluor 488 (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... we washed the sections and incubated them in PBST containing the corresponding secondary antibody tagged with fluorophores that match our microscope filters (1:300, Alexafluor 488 goat anti-rabbit, A11008, Lot # 2557379, Alexafluor 555 donkey anti-sheep, A 21436, Lot # 2420712 ThermoFisher Scientific, US), 1% NGS and 1% NDS ...
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bioRxiv - Immunology 2024Quote: ... Cell proteins and EVs proteins were quantified by Qubit Protein Assay (Invitrogen) using a Qubit 3.0 Fluorometer (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... and recombinant SHH was purified with Detoxi-Gel™ Endotoxin Removing Gel (ThermoFisher) and dialyzed ...
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bioRxiv - Plant Biology 2020Quote: All recombinant plasmids were generated by the GATEWAY cloning system (Invitrogen, http://www.invitrogen.com/). The primers used for gene amplification are listed in S3 Table ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant lentivirus was produced by transfecting HEK293T cells with Lipofectamine 2000 (Invitrogen, 11668019) or TransIT-2020 transfection reagent (Mirus ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant adenovirus plasmids were homologously recombined with pAd/CMV/V5-DEST vectors (Invitrogen), as per manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... and 50 ng/ml human recombinant M-CSF (ThermoFisher Scientific, Rockford, IL, USA). After six days of PBMC culture ...
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bioRxiv - Developmental Biology 2020Quote: ... Recombinant lentiviruses were produced by transient transfection in HEK293FT cells (Invitrogen, CA, USA), as described earlier 18 ...
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bioRxiv - Genomics 2019Quote: ... 4) We used RNase Inhibitor in place of RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen) in the first-strand RT reaction ...
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bioRxiv - Microbiology 2021Quote: ... 10 ng/ml of recombinant human fibroblast growth factor 2 (FGF2, Life Technologies), 20 ng/ml of recombinant human epidermal growth factor (EGF ...
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bioRxiv - Cell Biology 2020Quote: ... except that a final concentration of 10 nM recombinant PKR (Life Technologies # PV4821) and 1 mM ATP was added during incubation with FLAG M2 magnetic beads ...
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bioRxiv - Bioengineering 2021Quote: ... The cell passage was performed using recombinant cell-dissociation enzyme (TrypLE, Gibco™) to detach cells followed by neutralizing with culture medium ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant aFGF/FGF-1 and bFGF/FGF-2 were purchased from Thermo Fisher Scientific (13241-103 and PHG0024) ...
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bioRxiv - Biophysics 2020Quote: ... and stimulated with 50 U/mL recombinant interleukin-2 (IL-2, Thermo Fisher) and 5 μg/mL phytohaemagglutinin (PHA ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 10 ng/ml.
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bioRxiv - Cell Biology 2021Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 10 ng/ml.
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bioRxiv - Microbiology 2020Quote: ... coli-derived recombinant BAG1) and secondary goat anti-rabbit 594 (1:1000, Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Biophysics 2020Quote: ... Recombinant constructs were co-expressed with BirA in E.coli BL21 DE3 cells (Invitrogen) in Luria Bertani media supplemented with 50 mM biotin ...
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bioRxiv - Microbiology 2022Quote: ... the recombinant 3CLpros were expressed in Escherichia coli BL21 cells (Invitrogen, Carlsbad, CA) grown in Luria Bertani broth induced with 1mM isopropyl ß-D-thiogalactopyranoside ...
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bioRxiv - Developmental Biology 2022Quote: ... The recombinant plasmids were introduced into HEK293-EBNA cells (Invitrogen, Carlsbad, CA, USA) using FuGENE 6 transfection reagents (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... Recombinant zebrafish Nid2a and Hmcn1 fragments were generated in HEK293-EBNA cells (Invitrogen) and purified as described above for the mouse proteins and used to immunize rabbits or guinea pigs (Pineda Antikörper Service ...
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bioRxiv - Neuroscience 2021Quote: ... recombinant TTR was detoxified using a high-capacity endotoxin removal resin (Thermo Scientific) and quantified using the Lowry based DC Protein Assay (Bio-Rad Laboratories).
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bioRxiv - Microbiology 2020Quote: ... the recombinant plasmids were linearized with PacI before transfection with Lipofectamine 2000 (ThermoFisher) into the adenovirus packaging cell lines HEK293 cells ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 20 U/ml RNase-free Recombinant DNase I (Invitrogen, Cat # AM2235) and incubated for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant BACs were purified using PureLink™ HiPure Plasmid Midiprep Kit (ThermoFisher Scientific) and injected into 1-cell stage zebrafish embryos together with I-SceI (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were expanded on human recombinant vitronectin in StemFlexTM medium (Thermo Fisher Scientific). iBEC differentiation was performed as previously described by us [18] ...
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bioRxiv - Microbiology 2019Quote: ... cells were activated by adding 50 ng/mL recombinant mouse IFN gamma (Invitrogen). On day 7 ...
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bioRxiv - Plant Biology 2021Quote: ... The constructs were used for generating recombinant baculovirus in Sf21 insect cells (Invitrogen). RBA1 and RBA1 mutant proteins were expressed in Sf21 insect cells with recombinant baculovirus infection at 28 °C for 48 hr ...
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bioRxiv - Immunology 2021Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 μL (20 U) of RNaseOUT™ Recombinant Ribonuclease Inhibitor (Life Technologies, USA) was added to each aliquot and inverted to mix ...
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bioRxiv - Neuroscience 2020Quote: ... to enhance viral expression (primary antibody: GFP Recombinant Rabbit Monoclonal Antibody, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human EGF and PDGF-BB were purchased from Gibco (Catalog No. PHG0311) and Peprotech (Catalog No ...
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bioRxiv - Cell Biology 2020Quote: ... the nucleotide sequence was inserted by means of a BP recombinant reaction (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: Recombinant GST and GST-tagged EB1 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.7 and induced with 1 mM IPTG for 5.5 h at 25°C ...