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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for Recombinant Sheep IL12B Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cells were then washed and incubated with anti-rat Ab magnetic beads at 1 bead/target cell for 40 min at 4C (Dynabeads sheep anti-rat IgG, Invitrogen). Cell suspensions were stained with Abs specific for congenic markers ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed and incubated with anti-rat Ab magnetic beads at 1 bead/target cell for 40 min at 4C (Dynabeads sheep anti-rat IgG, Invitrogen). Cells were sorted into 3mL of complete media (RPMI 1640 ...
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bioRxiv - Biophysics 2022Quote: ... polyclonal anti-human MPO (IgG, sheep, 1:50) (AA16-718, Antibody online), monoclonal anti-human Histone H1 (IgG2a, mouse, 1:100) (MA5-13750, Invitrogen), or monoclonal anti-human NPM1 (IgG1 ...
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bioRxiv - Physiology 2022Quote: ... Secondary antibodies used were mouse anti-rabbit (1:10’000, Jackson, 211-032-171) and rabbit anti-sheep (1:10’000, Invitrogen, 81-8620).
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bioRxiv - Microbiology 2022Quote: ... Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922) were passaged twice on tryptic soy agar with 5% sheep’s blood (Thermo Scientific) at 37° C in 5% CO2 for 18-24 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... was grown overnight on blood agar plates (with 5% sheep blood) or in brain heart infusion broth (SA, ThermoFisher Scientific) until OD600=0.4 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed in PBS three times and incubated in a PBST solution containing the secondary antibody (Alexa Fluor 647-conjugated donkey anti-sheep, 1:1000, A21448, Invitrogen) and nuclear dye ...
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bioRxiv - Genetics 2023Quote: ... Samples were incubated for 2 hours at 4°C with rotation before adding 50 µL sheep anti-mouse IgG dynabeads washed in IPP buffer (Invitrogen). The unbound fraction was retained ...
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bioRxiv - Microbiology 2024Quote: Additional homogenised tumour tissues from four patients (PT09, PT14, PT15, PT17) were also cultured on sheep blood agar plates (Thermofisher) in an anaerobic hood at 37°C condition ...
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bioRxiv - Developmental Biology 2024Quote: ... blocking with 5% heat-inactivated sheep serum and incubation with an anti-digoxigenin antibody conjugated to alkaline phosphatase (Invitrogen, AM1326). NBT/BCIP (4-nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolylphosphate ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial cultures were plated on THM-blood-agar consisting of 5% of sheep blood (Remel Lenexa, KS) in THM with 1.5% agar (Fisher Scientific). The bacteria were routinely cultured in an anaerobic chamber in an atmosphere consisting of 80% N2 ...
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bioRxiv - Bioengineering 2024Quote: ... at a 1:200 dilution for 2 h at room temperature (RT) followed by donkey anti-sheep Alexa Fluor 647 secondary antibody (Invitrogen) at a 1:400 dilution and donkey anti-mouse Alexa Fluor 555 antibody (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were incubated with 1:200 donkey-anti-sheep (A11015, MoBiTec) and 1:1000 donkey-anti-rabbit (A21206; Invitrogen) secondary antibodies for 1 h at RT and washed 3 times ...
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bioRxiv - Evolutionary Biology 2023Quote: ... This step was followed by incubation with 50μl of Dynabeads® M-280 sheep anti-mouse IgG (11201D, Thermo Scientific) for 3 hours ...
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bioRxiv - Microbiology 2023Quote: ... Afterwards the blots were incubated (1:5000 dilution) with a sheep anti-mouse horseradish peroxidase-conjugated antibody (Life Technologies, France). The proteins were revealed using an ECL Western Blotting Substrate (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... the brains were placed in the secondary antibody solution containing 1xPBS/0.1% Triton X-100/3% donkey serum and a 1:1000 dilution of donkey anti-sheep 647 (A-21448, Thermofisher) for 10 days.
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bioRxiv - Microbiology 2023Quote: ... serial dilutions of the cryopreserved homogenates were plated on eight different media: CBA (Columbia Blood Agar supplemented with 5 % defibrinated sheep blood (Thermofisher)) ...
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bioRxiv - Microbiology 2023Quote: ... The culturing for all the samples was done onto tryptic soy agar with 5% sheep’s blood (Thermo Fisher Scientific, USA). The plates were incubated at +35±2°C in 5% CO2 and read at 24-hour intervals for four days ...
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bioRxiv - Immunology 2023Quote: ... The depletion of endogenous microbiota was monitored by conducting quantitative fecal cultures on Sheep Blood agar plates (Thermo Scientific, #R01200). This allowed the assessing of the reduction in microbial colony-forming units (CFUs ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies were used at 1 μg mL-1 for goat anti-rabbit and sheep anti-mouse linked horseradish peroxidase (Invitrogen). Blots were developed with SuperSignal West Pico Chemiluminescent Substrate (Pierce).
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bioRxiv - Microbiology 2023Quote: ... for 16 h at 4°C and with a secondary fluorescent antibody (Alexa-488 anti-sheep diluted 1:50,000; Thermo Fisher) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was sheared using a Bioruptor sonicator for four sets of 15 × 30s at amplitude of 50% with 30s shut off intervals and immunoprecipitated using sheep anti- mouse conjugated Dynabeads (Invitrogen) coupled with 1:10 mouse anti MYC antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... primary antibodies targeting SARS-CoV-2 proteins were used at indicated dilutions / concentrations (Table 1, MRC PPU Dundee) before staining with Donkey anti-Sheep AF488 Secondary Antibody (Invitrogen) at 1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then washed and incubated in secondary antibody (donkey anti-rabbit Alexafluor 488, Invitrogen, Carlsbad, CA A-21206 1:500; donkey anti-sheep Alexafluor 647, Invitrogen, A-21448 1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-MRC2 (as above) as a primary antibody with a secondary of Donkey anti-sheep conjugated to a fluorophore (Invitrogen). Cells were then analyzed and/or sorted on one of the above devices ...
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bioRxiv - Microbiology 2023Quote: ... Cells stained with anti-ACE2 and anti-GD3 were further incubated with secondary antibodies Alexa Fluor 488-labeled sheep anti-goat and goat anti-mouse (Invitrogen) for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg/µL of anti-Rpb3 or anti-Rpb4 antibody was incubated with 25 µL of magnetic beads (Dynabeads M-280 Sheep anti-Mouse IgG, 11202D, Invitrogen) for 1 h at 4°C in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... and immediate collection of bead bound RNA and transcription complexes in Trizol following immunoprecipitation by Rpb1 CTD (4H8) mouse mAB conjugated to Dynabeads M-280 sheep anti-mouse IgG (ThermoFisher). mNET-seq experiments were completed in biological replicate ...
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bioRxiv - Immunology 2023Quote: ... and either goat anti-rabbit IgG-AlexaFluor 647 (A-21245) or donkey anti-sheep IgG-AlexaFluor 647 (A-21448, all from ThermoFisher). Cells were washed twice with 0.1% Tween20 in PBS and once with PBS containing DAPI to visualize the nucleus ...
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bioRxiv - Molecular Biology 2024Quote: ... We followed the kit protocol except for conjugating 5µg of anti-total RNAPII to sheep anti-mouse dynabeads (Life Technologies). Sequencing libraries were generated using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina.
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bioRxiv - Genomics 2024Quote: ... Cryosections were incubated for 1h in secondary antibody (1:1000 donkey anti-sheep conjugated with AlexaFluor-488; ThermoFisher Scientific (Invitrogen)) ...
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bioRxiv - Molecular Biology 2024Quote: ... we prepared for each sample and IgG control: 120□μl of Dynabeads M-280 Sheep anti-mouse IgG (Thermo Fisher) or 40□μl of Dynabeads protein G (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... Cells were then isolated as CD4 or CD8 respectively with immunomagnetic beads (Dynabeads Mouse CD4 +, Invitrogen, catalog: 11445D; or Dynabeads Sheep anti-Rat IgG, Invitrogen, catalog ...
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bioRxiv - Genomics 2024Quote: ... Cryosections were incubated for 1h in secondary antibody (1:1000 donkey anti-sheep conjugated with AlexaFluor-488; ThermoFisher Scientific (Invitrogen)) ...
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bioRxiv - Microbiology 2020Quote: ... with 0.1 ng/mL human recombinant epidermal growth factor (EGF; Gibco) and 0.05 mg/ml bovine pituitary extract (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... and human recombinant insulin (2.5 µg/mL, Thermo Fisher Scientific, 12585014). From Day 1 to 11 ...
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bioRxiv - Cell Biology 2020Quote: ... Ki-67 recombinant rabbit monoclonal antibody (SP6) (Thermofisher, Cat. #: MA5-14520), Goat anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant soluble ACE2 was coated on NUNC Maxisorp plates (Thermo Scientific) at 1µg/well at RT for 3 h ...
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bioRxiv - Biochemistry 2020Quote: ... Full-length recombinant human LRRK2[G2019S] was purchased from Invitrogen (#A15202). GZD-824 was purchased from Cayman Chemical (#21508 ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant gene was cloned into pFastBac1 expression vector (Life Technologies) for bacmid production ...
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bioRxiv - Cell Biology 2019Quote: Recombinant Bax was labeled by NBD dye (IANBD amide, Life Technologies) as reported previously (Kale et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... and 2.5 ng/mL of recombinant human IL-2 (Thermofisher, PHC0027). T-cells were co-cultured with the mCherry-Nucleus-7 MCF7 cells in the presence of CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher ...
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bioRxiv - Genetics 2021Quote: ... 50 ng/ml recombinant mouse epidermal growth factor (EGF; Gibco, PMG8041), 100 ng/ml recombinant murine Noggin (PeproTech ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 ng/mL of human recombinant epidermal growth factor (Invitrogen PHG0313), 10 ng/mL of basic fibroblast growth factor (Invitrogen PHG0263 ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 5 ng/mL recombinant human interleukin 2 (Gibco, #PHC0027), 2 mM L-glutamine (Lonza ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 ng/ml recombinant human FGF-Basic (Thermo Fisher Scientific) in a humidified incubator with 5% CO2 at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... the isolated recombinant EMBacY was transfected into adhesive Sf9 cells (Invitrogen) in 6-well plates ...
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bioRxiv - Microbiology 2020Quote: Recombinant human mAbs were expressed in Expi293 HEK cells (Life Technologies), which were maintained in suspension at 37°C and 8% CO2 ...