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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Recombinant Sheep IL12B Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... supplemented with 294.4 ng/l human recombinant Epidermal Growth Factor (EGF) (Gibco, 10450-013), 25 mg/l Bovine Pituitary Extract (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... the epidermis was separated from the dermis and incubated in TrypLETM recombinant enzyme (ThermoFisher) for 1h and subsequently dissociated mechanically with a pipette tip ...
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bioRxiv - Immunology 2023Quote: ... The recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculoviruses were generated by transfecting purified bacmid DNAs into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Developmental Biology 2023Quote: ... Recombinant anti-GFP nanobody was conjugated to epoxy-activated magnetic Dynabeads M270 (Life Technologies) using 10 μg recombinant protein/1 mg Dynabeads ...
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bioRxiv - Immunology 2023Quote: ... Recombinant RBD (Arg319 – Phe541) of the same strain was obtained from Invitrogen (# RP 87678) and used as an immunogen without conjugation with KLH and used for screening assays ...
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bioRxiv - Immunology 2023Quote: ... Recombinant antigen was immobilized on N-hydroxysuccinimide (NHS)–activated agarose resins (Thermo Fisher, 26197) by overnight rotation at 4°C ...
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bioRxiv - Cell Biology 2024Quote: Recombinant tau monomers (human 4R2N T40/ Evotec, Germany) were labeled with Cy3 (Life Technologies). To prepare tau oligomers ...
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bioRxiv - Neuroscience 2024Quote: ... An injection of Cholera Toxin Subunit B (recombinant) conjugated to Alexa Fluor 647 (ThermoFisher) was made in the striatum of the same hemisphere (+1.2mm AP ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 μg/L of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (16) ...
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bioRxiv - Immunology 2024Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Thermo Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Recombinant human PDZD8 (1-28) - FLAG was expressed in Expi293 Cells (Thermo Fisher Scientific) using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen, Waltham, MA, USA). 5 µg of each protein fraction was added to 1x loading buffer (250 mM Tris pH 6.8 ...
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bioRxiv - Neuroscience 2020Quote: Total protein concentrations of isolated protein lysates were quantified by BCA protein assay kit (ThermoFisher) and equal protein amounts were boiled at 95°C for 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Cell Biology 2021Quote: ... Total proteins were quantitated using Qubit Protein Assays (ThermoFisher) and equal loads of total protein were separated on a NuPAGE 4-12% Bis-Tris mini gel (NP0336BOX ...
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bioRxiv - Neuroscience 2022Quote: ... BCA protein assay kit (ThermoFisher Pierce BCA Protein Assay) was used to determine the protein concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was quantified by BCA Protein Assay (Thermo Scientific) and 50 µg of total protein in 100 mM TEAB and 10% acetonitrile was incubated at 99°C for 1 h.
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bioRxiv - Genetics 2020Quote: ... protein quantification using BCA Protein Assay Kit (Thermo Fisher) was done ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were immunoprecipitated with Protein A Dynabeads (ThermoFisher) conjugated to the relevant antibody (anti-MqsRA ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified using BSA Protein Assay Kit (ThermoFisher). Equal amounts of protein were boiled with 6X loading buffer (375mM Tris HCL ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration was measured using BCA Protein Assay (ThermoFisher). For anti-NP immunoprecipitation ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined with Bradford protein assay (Thermofisher), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cas9 protein (Fisher Scientific A36498 Truecut Cas9 Protein v2) and gRNA were co-electroporated into G1 ES cells derived from an F1 hybrid blastocyst of 129S6 x C57BL/6J ...
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Allelic diversity uncovers protein domains contributing to the emergence of antimicrobial resistancebioRxiv - Microbiology 2022Quote: ... alongside protein standards (SeeBlue Pre-stained Protein Standard, Invitrogen). Gels were run at room temperature for 150 min at 80 V in running buffer ...
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bioRxiv - Plant Biology 2022Quote: ... the magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4°C for 2 hours ...
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bioRxiv - Plant Biology 2023Quote: ... the magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and mixture was rotated at 4°C for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... cas9 protein (Fisher Scientific A36499 TRUECUT CAS9 PROTEIN V2) and the gRNA with concentrations of 10 ug ...
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bioRxiv - Biochemistry 2023Quote: ... protein quantified by BCA protein assay ( Thermo scientific #23250), and 30 μg of the proteins were loaded on 4-12% Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration was determined by Qubit protein assay (Invitrogen) in a Qubit 4 fluorometer (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... 200 μL of Protein A (Dynabeads Protein A, Invitrogen) beads were washed twice in coIP buffer containing PIs ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were quantified using Coomassie Protein Assay Reagent (ThermoFisher). Protein samples (30 μg ...
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bioRxiv - Microbiology 2019Quote: All strains were cultured for 16-20 hours (B. anthracis) or 24 hours (Y. pestis) on BD BBLTM Trypticase Soy Agar II with 5% sheep blood (SBA) (Thermo Fisher Scientific, Waltham, MA, USA) at 35°C in ambient air from glycerol stocks stored at −170°C.
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bioRxiv - Microbiology 2019Quote: The 51 Salmonella isolates were struck for isolation from the frozen stocks to Tryptic Soy Agar (TSA) with 5% sheep blood (BAP) (Thermo Fisher Scientific Inc, Waltham, MA) and incubated overnight at 37°C to ensure purity ...
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bioRxiv - Cell Biology 2019Quote: ... 18 and 19 or TSS were pre-cleared by incubation with 1 × 107 Dynabeads magnetic beads conjugated to sheep α-mouse IgG (Invitrogen Dynal AS, Oslo Norway) at 4°C for 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... and the deletion mutant 04-309 ΔpamZ were cultivated as follows: bacteria were grown on Columbia sheep blood agar (CSA, Thermo Fisher Scientific Oxoid, Schwerte, Germany) medium plates at 37°C for 2-3 days ...
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bioRxiv - Neuroscience 2023Quote: ... was analyzed by indirect immunofluorescence staining using sheep anti-AnkB (Qu F et al. 2016) and AlexaFluor secondary antibodies (Thermofisher #A-11039, RRID: AB-2534096). Stimulated emission depletion microscopy (STED ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction obtained by centrifugation at 15,000 rpm for 15 min was reacted with sheep anti-mouse or anti-rabbit primary antibody-conjugated Dynabeads M280 (Thermo Fisher Scientific, Waltham, MA, USA) for 4 h or overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A-tagged proteins were detected with HRP-conjugated polyclonal anti-Protein A (Invitrogen, PA1-26853) at 1:5000.
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were resolved alongside a protein ladder (PageRuler Plus Pre-stained Protein Ladder, Thermo Fisher Scientific) on NuPAGE 4-12% BisTris gradient gels using NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration was quantified using Qubit™ Protein and Protein Broad Range (BR) Assay Kits (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... and protein concentration was determined by protein assay (EZQ Protein Quantification Kit; Life Technologies, Carlsbad, CA). Ct226-FLAG was affinity purified using magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... The cells were then stimulated with 20 ng/mL of recombinant human IL-4 (Gibco) and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... cells were co-stained with recombinant Cholera toxin subunit B conjugated to Fluor488 (Invitrogen C34775) at 1 μg/mL for less than 1 minute prior to fixing and staining for NINJ1 as described above ...
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bioRxiv - Physiology 2019Quote: Recombinant adenoviruses were generated using the pAd/CMV/V5-DEST Gateway recombination system (Life Technologies) after cloning the full-length cDNA into the pENTR vector ...
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bioRxiv - Biochemistry 2019Quote: ... The resulting antiserum was affinity purified using recombinant CTLMA2 immobilized on Aminolink resin (Thermo Fisher). Western blotting was performed with affinity purified anti-CTLMA2 ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant BACs were isolated from the bacterial hosts and used to transfect Sf9 cells (Invitrogen).
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bioRxiv - Biochemistry 2021Quote: ... The recombinant IgG (XBA14 and XBA18) was produced in FreeStyle 293 cells (Thermo Fisher Scientific), and purified by Protein A-Sepharose (Cytiva ...
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bioRxiv - Bioengineering 2019Quote: ... and genomic DNA was digested using Optizyme™ recombinant DNase-I digestion kit (Thermo Fisher) per manufacturers’ instructions ...
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bioRxiv - Biochemistry 2021Quote: GST-tagged p107 constructs loaded on beads were phosphorylated with recombinant Cyclin A/CDK2 (Invitrogen) in 200 nM ATP KAS buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Cancer Biology 2020Quote: ... One ng/mL recombinant human interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), was added to the media of the IL-6 dependent myeloma cell lines INA-6 ...