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Citations for Thermo Fisher :
1151 - 1200 of 6974 citations for Recombinant Human OXSR1 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... and 1 μL purified GST-FDL1 protein were incubated at room temperature for 20 min and loaded on a 6% DNA retardation gel (Life Technologies) before transfer to a nylon membrane ...
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bioRxiv - Molecular Biology 2020Quote: GST or GST-PHF1-tudor proteins (10 μg each) were incubated with 4 μl (25% slurry) of Glutathione Magnetic Beads (88821; Thermo Scientific) in 250 μl of binding buffer (125 mM Tris-HCl pH8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... the pooled anion exchange fractions were phosphorylated in vitro overnight at 4 °C with 1 mM ATP and 1 μg of PKR(252-551)-GST enzyme (Thermo Scientific) per mg of eIF2α ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...
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bioRxiv - Immunology 2019Quote: ... Lysates and purified products were analyzed by acrylamide gel electrophoresis followed by standard coomassie blue staining and western blot analysis using a rabbit anti-GST Tag polyclonal antibody (CAB4169, Thermo Fisher).
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bioRxiv - Microbiology 2020Quote: ... Two and a half micro liter of pBAD-C+SPA+gst sample as well as 5 μL of Spectra Multicolor Low Range Protein Ladder (Thermo Scientific) and 10 μl of samples or controls were loaded on tricine-SDS-gels (Schägger ...
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bioRxiv - Neuroscience 2019Quote: Full-length Eya1 wild type and mutant D328A protein in a GST-fusion format were generated using the Bac-to-Bac Baculovirus Expression system (ThermoFisher Scientific) and expressed in Sf9 insect cells ...
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bioRxiv - Biochemistry 2020Quote: ... ELISA wells were washed four times with PBS-T and incubated with 50 μL/well of anti-GST (Invitrogen MA4-004) or anti-MAR (Millipore-Sigma MAB1076 ...
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bioRxiv - Biochemistry 2023Quote: ... HP1 proteins was released from the GST-tag by overnight thrombin digestion followed by purification on a 50HQ 10×100 column (Applied Biosystems) in a continuous gradient of 50 –1000 mM NaCl ...
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bioRxiv - Pathology 2023Quote: The in vitro pull-down assay to assess the protein-protein interaction between YcgR and SpeA was conducted as previous described by using the Pierce GST Protein Interaction Pull-Down Kit (Thermo Fisher)(68) ...
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bioRxiv - Neuroscience 2023Quote: ... 200 µg of the proteins was incubated with equivalent amount of GST-α synuclein fusion protein at 4°C overnight in the present of Ni-NTA beads (Invitrogen). The pull-downs were washed 4 times with lysis buffer and boiled in 2X Laemmli sample buffer for five minutes.
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bioRxiv - Biochemistry 2023Quote: ... Interaction of His-fused proteins with other purified proteins (e.g. GST-PKAC) or synthetic peptides was studied by pulldown using Ni-NTA affinity resin (HisPur, Thermo scientific # 88221). GST-fused proteins were pulled down on glutathione-Sepharose beads (Amersham Biosciences) ...
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bioRxiv - Cell Biology 2024Quote: The binding affinity of Halo-CCSer2650–850 for Ndel1 was determined by coupling GST-Ndel1 to 30 μL of Glutathione Beads (Thermo Scientific) in 2 mL Protein Lo Bind Tubes (Eppendorf ...
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bioRxiv - Plant Biology 2020Quote: ... To detect V5-tagged proteins a 1: 5,000 dilution of the primary anti-V5 antibody (Invitrogen, #R96025) and an anti-mouse horseradish peroxidase-conjugated secondary antibody (1:10,000 dilution ...
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bioRxiv - Biophysics 2021Quote: ... The GFP-tagged and the knockout strains come from the commercial Yeast GFP clone collection (ThermoFisher Scientific). All cultures were grown in YPD medium (1% Yeast Extract ...
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bioRxiv - Genetics 2021Quote: ... The ORF was Gateway cloned into a C-terminal GFP-tagged Gateway pcDNA-DEST47 vector (ThermoFisher Scientific), sequence verified ...
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bioRxiv - Molecular Biology 2021Quote: ... HA-tagged hamster SCAP was prepared by subcloning PCR products into the pcDNA5/FRT/TO vector (Invitrogen). DsRed-ER plasmids were purchased from Addgene (#55836) ...
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bioRxiv - Biochemistry 2019Quote: ... 5 μg (otherwise noted) of terminally tagged and unmodified σ1R plasmid was transfected using lipofectamine 2000 (Invitrogen) for HEK 293T Δσ1R cells in a 10 cm plate ...
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bioRxiv - Neuroscience 2019Quote: ... GFP-Msp300KASH tagged OSN nuclei were pulled down using a Chicken anti-GFP antibody (Invitrogen #PA1-9533) bound to magnetic Dynabeads™ Protein G (Invitrogen #10003D) ...
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bioRxiv - Microbiology 2019Quote: ... coli MG1655 cells expressing tagged HupA-GFP with 2 µg/ml of FM4-64 dye (Invitrogen, USA) and incubating for 5 min at 37°C with shaking at 180 rpm ...
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bioRxiv - Biochemistry 2019Quote: His-tagged SIVsmm Nef constructs were expressed in BL21 (DE3) star cells (Life technologies, Grand Island, NY), and induced with 0.3 mM IPTG at 25 °C overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... EdU was tagged with Alexa Fluor 647 picolyl azide through click reaction (Click-iT chemistry, ThermoFisher, C10640). The cells were blocked with blocking buffer at least overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Pierce anti-HA beads for immunoprecipitation of HA-tagged GAPDH was purchased from Thermo Fisher (Cat. # 88836) Rabbit polyclonal antibody (Cat ...
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bioRxiv - Neuroscience 2021Quote: ... Epitope-tagged proteins were eluted from beads by boiling for 5 min in LDS sample buffer (ThermoFisher). Anti-D-cysteine immunoprecipitates were eluted by incubating beads in 1 mM D-cys in TBS for 30 min at room temperature ...
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bioRxiv - Physiology 2021Quote: ... incubated with different primary Abs and revealed by appropriate Alexa-Fluor-tagged secondary Abs (Thermo Fisher Scientific). Cells were analyzed by using a Nikon Ti Eclipse inverted microscope with A1 scanning confocal microscope at the Confocal and Specialized Microscopy Shared Resource (CSMSR) ...
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bioRxiv - Plant Biology 2021Quote: ... Purified His-tagged proteins were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris Protein Gels, Invitrogen) and gels were stained with Quick Coomassie Stain (Generon) ...
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bioRxiv - Cell Biology 2021Quote: ... incubated with different primary Abs and revealed by appropriate Alexa-Fluor-tagged secondary Abs (Thermo Fisher Scientific). Slides were mounted in Fluoromount-G (Southernbiotech ...
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bioRxiv - Neuroscience 2020Quote: ... The fluorescent-tagged secondary antibodies Alexa Fluor 488 and Alexa Fluor 555 (1:500, Thermo Fisher Scientific) were used for detection ...
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bioRxiv - Cell Biology 2021Quote: cDNAs in pENTR or pDONR were transferred into tagged mammalian expression vectors using Gateway® recombination (Invitrogen): pCAG-DEST-EGFP ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were transfected with 200 ng of Flag-tagged FOXQ1 using Lipofectamine 2000 (Thermo Fisher, Waltham, USA). After 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... His-tagged proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific Cat. Nr. 88222) according to manufacturer’s instructions (batch protocol) ...
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bioRxiv - Microbiology 2020Quote: ... His tagged proteins were isolated from the supernatant using Dynabeads™ His-Tag Isolation and Pulldown (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Species-specific secondary antibodies tagged with Alexa Fluor corresponding to 568 nm emission spectra (1:1,000, Thermofisher) was used for immunofluorescence ...
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bioRxiv - Microbiology 2022Quote: ... HNRNPUL1 and MECR were recombined into V5-tagged mammalian expression constructs by Gateway cloning into pcDNA6.2 (Invitrogen) and pLX304 (Addgene 25890) ...
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bioRxiv - Microbiology 2021Quote: ... The His-tagged proteins were blotted using anti-6x His tag HRP-conjugated monoclonal antibody (Invitrogen, USA) and detected using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... Flag tagged proteins were immunoprecipitated from 2-3mg cellular extracts with Pierce Anti-DYKDDDDK Magnetic Agarose (Invitrogen). Beads were washed 5x with 1ml IP lysis buffer end eluted with 50µl 1xLaemmli buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μg of Flag-Strep-Strep-(FSS)-tagged LRRK2RCKW cDNA and Lipofectamine 2000 reagent (ThermoFisher Scientific, USA) were used according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... while non-Avi tagged antigens were biotinylated chemically using EZ-Link Sulfo-NHS-Biotin (Thermo Fisher, A39256). VH and VL sequences of SARS-CoV-2 control antibodies (COV2-2196 ...
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bioRxiv - Cell Biology 2021Quote: ... Cross-absorbed secondary antibodies tagged with Alexa-fluorophores were diluted in blocking buffer (1:400, Invitrogen, Switzerland) and applied for 30 min at RT ...
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher Scientific cat.# 88221). After washing by wash buffer (25 mM Imidazole ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 and MS2-tagged HEK293:24xMS2-NEAT1 cells were cultured in Dulbecco’s modified eagle’s medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... They were then transfected with Halo-tagged gene constructs using Lipofectamine LTX (Thermo Fisher Scientific, MA, USA). After 48 hours ...
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bioRxiv - Immunology 2024Quote: ... FLAG-tagged constructs were PCR amplified with Gateway adapters and cloned into pDONR221 using BP Clonase (Invitrogen). Hoil1 and Hoip mutations and deletions were generated using the Q5 site-directed mutagenesis kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged proteins were concentrated using 30K MWCO concentrators to ∼ 5 mg/ml (Thermo Scientific™, USA). Protein was stored at −80°C.
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bioRxiv - Developmental Biology 2024Quote: ... inserts were then subcloned into pCS2+ N HA tagged vectors that had been converted into Gateway (Invitrogen) destination vectors ...
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bioRxiv - Cell Biology 2024Quote: HEK cells and zebrafish samples were tagged with TMT10plex™ Isobaric Label Reagent Set (Thermo Scientific, #90111), and fibroblast cells with TMTpro™16plex Isobaric Labels (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... Hexahistidine-tagged proteins were then detected using mouse anti-6xHis tag antibody (Ma1-135, Invitrogen, Karlsruhe, Germany) as primary antibody and signals revealed by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (ab6789 ...
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bioRxiv - Immunology 2023Quote: ... Filtered supernatants containing His-tagged proteins were passed slowly through HisPur Ni-NTA Resin (Thermo Fisher 88221) beads in columns ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein A-tagged strains were immunoprecipitated with Rabbit IgG-conjugated (MP-Biomedicals, SKU 085594) Dynabeads (Invitrogen, 14301) (Vakiloroayaei et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... IPs of FLAG-tagged PRRC2B and PRRC2B fragments were performed using anti-DYKDDDDK Magnetic agarose (ThermoFisher Scientific). Beads were mixed with 70 μl of SDS loading buffer (Bio-Rad ...