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Citations for Thermo Fisher :
951 - 1000 of 6974 citations for Recombinant Human OXSR1 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... or ALS-associated Ruby-tagged sequences of DnaJC7 using 5 μL of Lipofectamine 2000 (Gibco) transfection reagent in OptiMEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged LCRIS_00661 and LCRIS_00558 genes with the appropriate restriction sites were ordered from Invitrogen and subcloned into a previously constructed S ...
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bioRxiv - Cancer Biology 2024Quote: ... Biotin-tagged peptides were labeled with Pierce™ High Sensitivity NeutrAvidin™-HRP (Thermo Fisher) directly after blocking ...
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bioRxiv - Microbiology 2024Quote: ... Vectors of sulfatase 1 and 2 tagged with myc were transfected using Lipofectamine 2000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... to assess FLAG-tagged protein expression and immunoprecipitation by Immunoblot and silver stain (ThermoFisher Scientific) respectively ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-tagged cDNA constructs and siRNAs were transiently transfected using the Lipofectamine 3000 (Invitrogen, #L3000001) as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... MBP-tagged protein was purified by affinity column chromatography using Ni-IMAC resin (Thermo Fisher) followed by size exclusion chromatography using a Superdex 200 column (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were visualised using a primary anti-6xhis antibody (ArtNr: 11533923, Fisher Scientific), a HRP-coupled secondary antibody (ArtNr ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotin-tagged ends were pulled down using Dynabeads MyOne Streptavidin C1 (Thermo Fisher Scientific 65001). Standard Illumina library preparation protocol including end repair ...
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bioRxiv - Biochemistry 2023Quote: Purified RRMs were fluorescently tagged using Alexa Fluor™ 633 C5 Maleimide (Thermo Fisher Scientific). The dye was added to a sample of purified protein (1:10 protein:dye molar ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc-tagged ACE2 or mAb were first immobilized on Protein G Dynabeads (ThermoFisher Scientific). The S protein solutions were then incubated with on-bead receptor or antibody at room temperature for 60 min ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from clonal CRISPR tagged cells using Genomic DNA Mini Kit (Invitrogen) according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... The His-tagged dAsapPZA protein was expressed and purified following the manufacturer’s guidelines (Invitrogen, USA).
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bioRxiv - Cell Biology 2024Quote: ... Secondary hybridization was performed using fluorescently tagged antibodies: AF647 goat anti-rabbit (A2l24S, Invitrogen, l:l000), AFS94 goat anti-rabbit (All037 ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit polyclonal anti-β-PIX antibody (4515S; Cell Signalling Technology) and rabbit polyclonal anti-GST antibody (71-7500; Invitrogen).
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bioRxiv - Plant Biology 2019Quote: ... the samples were subjected to SDS-PAGE followed by immunoblot analysis as described above using anti-GST antibody (Invitrogen).
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bioRxiv - Biochemistry 2019Quote: ... The supernatant corresponding to GST-tev-JMJD6 (1-403) was loaded to 7mL of glutathione agarose resin (Thermo Scientific), washed with nickel-binding buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Manuscript in preparation)] were transfected using DNA-In reagent (GST-2131, MTI Global Stem) or Lipofectamine 3000 (L3000075, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The MS/MS spectra were searched against GST-AIMP1 sequence database using Proteome Discoverer (version 1.3, Thermo Fisher Scientific) with SEQUEST search engine ...
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bioRxiv - Immunology 2022Quote: ... Phages presenting NLRP1PYD-specific VHHs were enriched using GST-NLRP1PYD immobilized to magnetic Pierce glutathione beads (Thermo Fisher Scientific). The retained phages were used to infect E ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were probed at 1:1000 dilution with mouse monoclonal anti-GST-DyLight800 conjugated antibody (ThermoFisher MA4-004-D800) and mouse monoclonal anti-His6 tag-DyLight680 conjugated antibody (ThermoFisher MA1-21315-D680) ...
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bioRxiv - Microbiology 2023Quote: ... Anti-GST pull-downs were completed with these protein suspensions subsequently incubated with Pierce™ Glutathione Agarose (ThermoFisher 16100) for an hour at 4°C in binding buffer (PBS pH 7.0 with 1 mM Tris (2-carboxyethyl ...
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bioRxiv - Cell Biology 2024Quote: Overexpression of glutathione S-transferase (GST) fusion proteins was induced in DH5α Escherichia coli cells (Life Technologies, MD, USA) using 0.4mM isopropyl β-D-thiogalactopyranoside and cells subsequently broken by sonication ...
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bioRxiv - Cancer Biology 2021Quote: ... human BNIP3 (Hs00969291_m1) and human 18S (Hs99999901_s1) were purchased from Applied Biosystems. 18S served as an internal control ...
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bioRxiv - Microbiology 2022Quote: Human monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (83) ...
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bioRxiv - Cell Biology 2020Quote: ... Ki-67 recombinant rabbit monoclonal antibody (SP6) (Thermofisher, Cat. #: MA5-14520), Goat anti-Mouse IgG (H+L ...
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bioRxiv - Genetics 2021Quote: ... and Mouse M-CSF Recombinant Protein were from Invitrogen (Carlsbad, CA). Lipopolysaccharides from Escherichia coli O111:B4 was purchased from Sigma (St Louis ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant soluble ACE2 was coated on NUNC Maxisorp plates (Thermo Scientific) at 1µg/well at RT for 3 h ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse IFN-alpha 2 (Ifna2) recombinant protein was purchased from Affymetrix eBioscience (catalog #14-8312) ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant gene was cloned into pFastBac1 expression vector (Life Technologies) for bacmid production ...
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bioRxiv - Cell Biology 2019Quote: Recombinant Bax was labeled by NBD dye (IANBD amide, Life Technologies) as reported previously (Kale et al. ...
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bioRxiv - Genetics 2021Quote: ... 50 ng/ml recombinant mouse epidermal growth factor (EGF; Gibco, PMG8041), 100 ng/ml recombinant murine Noggin (PeproTech ...
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bioRxiv - Biochemistry 2021Quote: ... the isolated recombinant EMBacY was transfected into adhesive Sf9 cells (Invitrogen) in 6-well plates ...
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bioRxiv - Biophysics 2022Quote: ... and 2 ng/mL recombinant murine interleukin-3 (IL-3) (Gibco). Cells were grown in a humidified incubator at 37 °C and 6% atmospheric CO2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 U of recombinant Taq DNA Polymerase (Thermo Fisher Scientific, Lithuania) and water to final volume of 10μl ...
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bioRxiv - Cancer Biology 2021Quote: ... and RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher, Cat. No. 10777019) and incubated on ice for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... We used GFP recombinant rabbit monoclonal antibody (G10362, Thermo Fisher Scientific) at 1:300 dilution and monoclonal anti-GFP ...
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bioRxiv - Immunology 2021Quote: ... and 1µl of RNaseOUTTM Recombinant Ribonuclease Inhibitor (Invitrogen, Catalogue no.-10777019). 11µl of the cocktail was added to the RNA/primer mix ...
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bioRxiv - Cancer Biology 2021Quote: ... and RNaseOUT™ Recombinant Ribonuclease Inhibitor (Thermo Fisher, Cat. No. 10777019) and the lysates were incubated on ice for 10 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... then treated with recombinant DNaseI (DNA-free DNA removal kit, Ambion) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Recombinant His-HMGB1 was produced in 293-freestyle cells (ThermoFisher Scientific). Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant protein was transiently expressed in Expi293™ (ThermoFisher Scientific, UK) and purified from culture supernatants by an immobilised metal affinity using an automated protocol implemented on an ÄKTAxpress (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant CEACAM-His proteins were expressed in Expi293F cells (Life Technologies) and purified by affinity chromatography (ÄKTA Pure ...
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bioRxiv - Immunology 2020Quote: ... Recombinant RBD proteins were produced in transfected FreeStyle 293F cells (Invitrogen) and purified by nickel affinity chromatography ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant soluble DPP4 was coated on NUNC Maxisorp plates (Thermo Scientific) at 100 ng/well at RT for 3 h ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were performed using Taq DNA Polymerase Recombinant kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... 40 μL/sample of recombinant Protein-G-Sepharose-4B beads (ThermoFisher) was washed thrice with IP buffer and added to each sample for equilibration on a rotator for 4h ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were produced using the Expi293F cells (Thermo Fisher Scientific) by transfecting 200×106 of these cells with purified DNA using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant ACE2-Fc (18-615) protein expressed in Expi293F (Life Technologies) cells was chemically biotinylated using EZ-link Sulfo-NHS-Biotin (A39256 ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant sACE2 protein was purified by nickel affinity columns (Invitrogen) and ACE2-Fc was purified using Protein A affinity column (Cytiva) ...