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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... NO --N and NO --N were determined by ion chromatography (Thermo Fisher, USA).
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bioRxiv - Immunology 2022Quote: ... cDNA encoding mouse TCR 2B4 α (with c-Myc-tag) and β (with V5-tag) sequences with 2A sequence linking each other were cloned into pCDNA3.1/Zeo(+) vector (Life Technologies) using Not1 and Xho1 restriction enzymes ...
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bioRxiv - Biochemistry 2021Quote: ... Coverslips were washed three time with PBS before the incubation with Alexa Fluor 555 (for Myc tag) and/or Alexa Fluor 647 (for HA tag) conjugated secondary antibodies (Invitrogen/Thermo Fisher) diluted in PBSAT for 30 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with SmaI and ligated in frame with SmaI a N-terminal 3xFLAG-tag and a C-terminal 6xhistidine tag into a modified and SmaI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH2.
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was digested with BsiWI and ligated in frame with a N-terminal 3 x FLAG-tag and a C-terminal 6 × histidine tag into a modified and BsiWI-digested pET101/D-TOPO expression vector (Thermo Scientific), yielding pET101-NanH1.
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed in 1 mL PBS and 100 µL of Phos-Tag lysis buffer was added dropwise to the well (Phos-Tag lysis buffer recipe: RIPA (ThermoFisher 89900), 3X Halt protease and phosphatase inhibitor cocktail (ThermoFisher 78444) ...
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bioRxiv - Immunology 2024Quote: ITS61.01 with a C-terminal rhodopsin tag (C9 tag) and untagged ITS103.01 were produced in transfected Expi293 cells (ThermoFisher, cat. A14527) and purified on rProtein A Gravitrap columns (Cytiva Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... plasmids containing centrobin constructs with a 5xMyc tag and TRIM37 constructs with a 3xFLAG tag were transfected into FreeStyle 293-F cells (Thermo Fisher) using FreeStyle MAX Reagent and OptiPRO SFM according to manufacturer guidelines (Thermo Fisher) ...
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bioRxiv - Genetics 2020Quote: ... The entire APN2 ORF containing the E59A mutation was synthesized by the GeneArt Gene Synthesis service (ThermoFisher Scientific) and cloned into a pGPD2 vector (Addgene #43972 ...
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bioRxiv - Developmental Biology 2020Quote: ... or V1-ORF-DEST (see below) via a Gateway LR Clonase reaction (as per manufacturer’s instructions; Life Technologies) to produce the following plasmids ...
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bioRxiv - Cancer Biology 2021Quote: ... All ORFs were cloned into pHAGE-IRES-GFP using pENTR™/D-TOPO™ Cloning Kit (Thermofisher Scientific). The virus package plasmids psPAX2 (Addgene plasmid 12260 ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK-293T-CC-ORFs were cultured as above with 0.3 µg/ml of puromycin (Gibco, Cat No. A1113803) in their culture medium.
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bioRxiv - Cell Biology 2023Quote: Primers sequences targeting the open reading frames (ORF) of genes of interest (GOI) were taken from the LeishGEdit primer database (www.LeishGEdit.net) and sourced from ThermoFisher. Due to high sequence similarity ...
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bioRxiv - Molecular Biology 2024Quote: ... Other ORFs were inserted into a pDONR223 by PCR amplification followed by BP reaction (Gateway recombination technology, Invitrogen) (see Table S1,S2 for details) ...
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bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
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bioRxiv - Immunology 2021Quote: ... virus-infected cells were detected using an in-house rabbit polyclonal antibody specific for SARS-CoV-2 N/M/E followed by horseradish peroxidase-conjugated goat anti-mouse IgG (Invitrogen). TCID50 was calculated using the Reed-Muench method.
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bioRxiv - Neuroscience 2022Quote: The reverse transcription was performed with 500 ng of total RNA using the High-capacity cDNA reverse transcription kit (ThermoFisher Scientific, catalog n°4368814) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: Guinea pig anti-zebrafish Rad21l1 polyclonal antibody production: An N-terminal fragment of Rad21l1 cDNA was amplified with Phusion DNA polymerase (Thermo Fisher Scientific, Catalog #: M0530L) using the following primers ...
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bioRxiv - Developmental Biology 2021Quote: ... for amplification of 100ng of cDNA template from N/D/S cells either treated with MS351 or vehicle using Power SYBR Green Master Mix (Thermo Fisher Scientific Cat. 4368577) qRT PCR protocol14 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained for 1h at RT with a monoclonal Anti-HA tag antibody [16B12] (ab130275, 1/100) and a secondary goat anti-mouse antibody labeled with Alexa 546 (Thermofisher #A-21123, 1/500) then counterstained with Prolong Gold mounting medium containing DAPI (Life Technologies #P36941).
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bioRxiv - Genomics 2022Quote: ... the HA-tagged protein of interest was detected with 1:500 diluted HA Tag Alexa Fluor 647 conjugated mouse antibody (ThermoFisher, Catalog no. 26183-A647). After antibody incubation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... NK-CD16 cells with positive SmBiT expression were selected via cell sorting by probing HA tag (anti-HA antibody [Invitrogen #26183, 1:500]; Anti-mouse secondary antibody Alexa Fluor 647 [Invitrogen #A-21235, 1:200]), probing CD16 (anti-CD16 antibody [Abcam #246222 ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were then incubated in secondary antibodies conjugated with an AlexaFluor tag (anti-mouse 647 and anti-rabbit 555, diluted 1:2000 and 1:5000 0.1% PBS-Tween, respectively, Thermo Fisher Scientific, Waltham, MA, USA) at room temperature for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated for 3 h at 4 °C with 50 mL of Pierce Protein-G beads bound with mouse anti-Ty antibody (Invitrogen Ty1 Tag Monoclonal BB2). Beads were washed 6 times with wash buffer (200 mM Tris pH 9 ...
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bioRxiv - Genetics 2020Quote: ... pAc5.1/V5-His version C (V411020, Thermo Fisher Scientific). In order to test the transferring ability of gypsy ...
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bioRxiv - Developmental Biology 2021Quote: ... and inserted into pMT/V5-His A (Thermo Scientific) using XhoI and SpeI target sites ...
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bioRxiv - Cell Biology 2022Quote: pAc5.1/V5-His vector (Thermo Fisher Scientific, Cat #V411020) was used to construct plasmids for expressing proteins or miRNAs in Drosophila S2 cells ...
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bioRxiv - Bioengineering 2020Quote: ... with 10% heat-inactivated FBS (HI-FBS; Life Technologies)) ...
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bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...
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bioRxiv - Biochemistry 2020Quote: ... the pBAD/His B vector (Invitrogen-Thermo Fisher, USA) was linearized by PCR with appropriate primers that contained an introduced ribosomal binding site and SalI and HindIII restriction sites to generate pBAD30_SalRBS ...
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bioRxiv - Neuroscience 2020Quote: ... heat-inactivated 10% fetal bovine serum (FBS HI, Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... and 9.9 μl of Hi-Di formamide (Applied Biosystems). These were then incubated for 5 min at 95°C and placed immediately on ice ...
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bioRxiv - Microbiology 2021Quote: ... Indicated amounts of recombinant GFP-His (Thermo Fisher # A42613) or SARS-CoV-2 N-His (Sino Biological # 40588-V08B ...
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bioRxiv - Immunology 2020Quote: ... and ligated into pcDNA3.1D/V5-His-TOPO vector (Invitrogen). Pseudoviruses were prepared by co-transfecting 1.25 µg of HIV-1 envelope containing plasmid with 2.5 µg of an envelope deficient HIV-1 backbone (PSG3Δenv ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies) and run on an Applied Biosystems 3730xl Genetic Analyzer ...
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bioRxiv - Biochemistry 2021Quote: ... Hi-5 cells (BTI-TN-5B1-4) (Gibco #B85502) were cultured in Express Five™ SFM (Serum-Free Media ...
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bioRxiv - Developmental Biology 2020Quote: ... and cloned into the pAc5.1-V5/His vector (Invitrogen). Immunoprecipitations between Bdbt-EGFP and Myc-DAnkrd49 used GFP rabbit serum (ab290 ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were cloned into pcDNA-V5/His (Invitrogen) using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... The pcDNA4-TO-myc/his B backbone vector (Invitrogen) was chosen for overexpression ...
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bioRxiv - Biochemistry 2024Quote: Supernatant was supplemented with His-Pur cobalt resin (ThermoFisher), imidazole to 10 mM ...
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bioRxiv - Microbiology 2023Quote: ... Indicated amounts of recombinant GFP-His (Thermo Fisher # A42613) or HCoV-OC43 N-His (Sino Biological # 40643-V07E ...
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bioRxiv - Cell Biology 2022Quote: ... were cloned in pcDNA3.1(-) Myc/His vector (ThermoFisher Scientific). Ligated plasmids were transformed in chemically competent E ...
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bioRxiv - Cell Biology 2023Quote: ... and into pcDNA™6/myc-His A (Invitrogen), from an amplicon produced by RT-PCR from S2Xpress RNA ...
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bioRxiv - Microbiology 2023Quote: ... araC and the araBAD promoter from pBAD/His (Invitrogen), the tetracycline resistance gene from pACE2 (Geneva Biotech ...
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bioRxiv - Immunology 2024Quote: His-tagged proteins were expressed in E.coli BL21 (Invitrogen) overnight at 37 °C in 50 mL auto-inductive Luria-Bertani medium ...
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bioRxiv - Bioengineering 2024Quote: ... 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco), 100 U ml−1 penicillin/100 μg ml−1 streptomycin (Gibco) ...
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bioRxiv - Biophysics 2024Quote: ... 1 nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher), and 400 nM FITC-labeled NCoR1 ID2 or MED1 ID2 peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Molecular Biology 2024Quote: ... and cloned into pcDNA3.1-myc/His A vector (Invitrogen) with restriction enzymes ...