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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... while the digestion cocktail for RNase HI-positive samples were supplemented with 10 U recombinant Escherichia coli RNase HI (Invitrogen). After digestion ...
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bioRxiv - Neuroscience 2021Quote: ... spanning exons 40-43) was cloned from mouse brain cDNA into pCRII-TOPO (Thermo Fisher). The plasmid was linearized and subjected to in vitro transcription with digoxigenin labeling (DIG RNA labeling kit ...
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bioRxiv - Cell Biology 2021Quote: ... total RNA from mouse liver was isolated and converted into cDNA using TRIzol (Thermo Fisher) and RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Amplified cDNA was hybridized on mouse Gene 2.0 ST arrays (Affymetrix, Santa Clara, CA, USA). Staining and scanning (Scanner 3000 7G ...
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bioRxiv - Cell Biology 2023Quote: Mouse cDNA was synthesized by reverse transcription from kidney RNA by M-MLV (ThermoFisher 28025013) with random primers (Invitrogen 48190011) ...
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bioRxiv - Plant Biology 2021Quote: ... The tagged PIN7 and PIN4 cDNA entry clones were then recombined with the modified pH7WG destination vector by the Gateway LR reaction (Invitrogen). For the cloning of the PIN7 splicing dual fluorescent reporter ...
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bioRxiv - Cell Biology 2021Quote: ... they were PCR amplified from available cDNA clones and recombined into pDONR221 using the BP reaction (Life Technologies, 11789-020) prior to being recombined into expression plasmids by LR cloning (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... encoding 11 hits were selected from the Human ORFeome library of cDNA clones (Open Biosystems) (29) and recombined into a FLAG (for FLAG tagging) vector using Gateway technology (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... they were PCR amplified from available cDNA clones and recombined into pDONR221 using the BP reaction (Life Technologies, 11789-020) prior to being recombined into expression plasmids by LR cloning (Life Technologies ...
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bioRxiv - Systems Biology 2023Quote: ... NSP16 and NSP10 mutant cDNA entry clones were generated by gene synthesis and subcloning into pDONR221 (GeneArt, Thermo Fisher Scientific). All cDNA clones were sequence verified and shuttled into LuTHy destination plasmids ...
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bioRxiv - Plant Biology 2023Quote: ... AHL10 wild type, phosphomimic, or phosphonull cDNA clones (described in Wong et al., 2019) were inserted into pDONR221 P1-P4 (Invitrogen) and the RRP6L1 cDNA was amplified from total mRNA and cloned into pDONR221 P3-P2 (Invitrogen) ...
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bioRxiv - Plant Biology 2024Quote: All fragments including the CENPC-k and CENPC motifs with and without DNA-binding regions (Supplementary Table 1-4) were amplified from the αKNL2 and CENP-C cDNA clones in pDONR221 (Invitrogen) vector using primers listed in Supplementary Table 5 ...
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bioRxiv - Molecular Biology 2020Quote: A pcDNA3.1/V5-His TOPO expression vector (Invitrogen) encoding the protein-coding sequence of human SM (NM_003129.4 ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 10% HI-FBS (16140-071, Gibco), 100 U/mL Penicillin-Streptomycin (15140122 ...
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bioRxiv - Biochemistry 2021Quote: ... then cloned into pIB/V5-His vector (Invitrogen), fused with V5 epitope at the C-terminus ...
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bioRxiv - Biophysics 2020Quote: ... 10 % v/v Hi-Di formamide (Thermo Scientific), supplemented with 2 mM vanadyl ribonucleoside complex) ...
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bioRxiv - Biochemistry 2021Quote: ... 10 % v/v Hi-Di formamide (Thermo Scientific), supplemented with 2 mM vanadyl ribonucleoside complex) ...
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bioRxiv - Cancer Biology 2020Quote: ... in 1X Hi-Fi PCR buffer (Invitrogen #52045) supplemented with 2 uL of 50 mM MgSO4 and dNTPs (Roche#11-581-295-001 ...
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bioRxiv - Genetics 2021Quote: ... and Hi - Di™ Formamide (Applied Biosystems, USA). The DNA (PCR products ...
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bioRxiv - Microbiology 2020Quote: ... MprA was detected using Anti-His antibody (Invitrogen) and anti-mouse HRP-labelled secondary antibody (Santa Cruz) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% (v/v) HI-FBS (Gibco), 10 U/mL penicillin and 100 U/mL streptomycin (Corning) ...
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bioRxiv - Immunology 2023Quote: ... 6x His Tagged monoclonal antibody (Thermo Fisher Scientific) was also plated as an experimental control ...
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bioRxiv - Biochemistry 2024Quote: Basal Conditioned medium: DMEM Hi Glucose medium (Gibco) supplemented with 1% Penicillin – Streptomycin – Glutamine (Gibco ...
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bioRxiv - Biochemistry 2024Quote: DMEM Starvation medium: DMEM Hi Glucose medium (Gibco) supplemented with 0.2%heat-inactivated fetal bovine serum (US origin ...
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bioRxiv - Biochemistry 2024Quote: DMEM complete medium: DMEM Hi Glucose medium (Gibco) supplemented with 1% Penicillin – Streptomycin – Glutamine (Gibco ...
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bioRxiv - Biochemistry 2024Quote: C2C12 differentiation medium: DMEM Hi Glucose medium (Gibco) supplemented with 2% Horse Serum (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% Hi-FBS (Thermo Fisher Scientific). For VP4 expression ...
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bioRxiv - Biochemistry 2023Quote: The plasmid pBAD/Myc-His A (pBAD) (Invitrogen) and pET28a (+ ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% HI-FBS and 2mM EDTA (Invitrogen, 15575038).
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bioRxiv - Immunology 2022Quote: ... 10% hi-FBS and 1% penicillin/streptomycin (ThermoFisher) in absence or presence of APRIL (Recombinant Human APRIL/TNFSF13 Protein ...
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bioRxiv - Microbiology 2022Quote: ... DMEM 2% Hi-FBS 0.5% Ultrapure agarose (Invitrogen) was used to overlay the wells ...
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bioRxiv - Biophysics 2023Quote: ... HRP-conjugated anti-His antibody (Invitrogen – Thermo Fisher), anti-H3 antibody (abcam ab1791 ...
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bioRxiv - Biophysics 2023Quote: ... HRP-conjugated anti-His antibody (Invitrogen – Thermo Fisher), anti-H3 antibody (abcam ab1791 ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (v/v) HI-FCS (Gibco), 50 μM mercaptoethanol (Sigma-Alrich) ...
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bioRxiv - Genetics 2023Quote: ... 8.85 μl of HI-DI Formamide (Applied Biosystems), and the reaction mix was adjusted to 50 μl using ddH2O ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 9% (v/v) HI-FCS (Gibco), 100 U/mL Penicillin and 100 µg/mL Streptomycin (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... Hi-DiTM formamide (Fisher Scientific, 44-407-53), phosphate buffered saline ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% HI-FBS (16140-071, Gibco), 100 U/mL Penicillin-Streptomycin (15140122 ...
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bioRxiv - Cell Biology 2023Quote: Primary antibodies used in western blotting (WB) and immunofluorescence (IF) were anti-6x His mouse monoclonal (Invitrogen, #MA1-21315, 1:5000 in WB), anti-GST mouse monoclonal (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol)ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N ′ -dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol) ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Immunology 2021Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020) ...
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bioRxiv - Immunology 2022Quote: ... 20 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES, Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... N,N-diisopropylethylamine (DIEA) and piperidine were from Acros Organics (Geel, Belgium). O-(6-Chlorobenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HCTU ...
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bioRxiv - Microbiology 2022Quote: ... and 1% HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid; Gibco). Wild type SARS-CoV-2 (isolate USA-WA1/2020 ...
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bioRxiv - Bioengineering 2024Quote: ... N,N-Dimethylformamide (DMF)) were ACS grade and purchased from Fisher Scientific.
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bioRxiv - Microbiology 2020Quote: ... The ORFs encoding for the selected proteins (Supp.Table 1) cloned into vector pDON223 (Gateway system; Invitrogen, USA) by recombination cloning (Gateway system ...
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bioRxiv - Genetics 2021Quote: ... The ORF was Gateway cloned into a C-terminal GFP-tagged Gateway pcDNA-DEST47 vector (ThermoFisher Scientific), sequence verified ...
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bioRxiv - Physiology 2021Quote: ... All full length ORFs were cloned into the pCR™4-TOPO®TA (Thermo Fisher Scientific) for sequence verification.
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bioRxiv - Cancer Biology 2021Quote: ... we generated an open reading frame (ORF) for CDA by PCR that was subcloned in pDonor221 (Invitrogen). CDA E67Q catalytically inactive mutant42 was generated by site directed mutagenesis according to the manufacturer instructions (New England Biolabs) ...