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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... and the PKN1 ORF without stop codon was cloned via a BP reaction into pDONR221 (Invitrogen, creating pDONR221-PKN1nostop). The pro35S:PKN1-GFP construct was created by recombining pDONR221-PKN1nostop via an LR reaction behind the CaMV 35S promoter and in front of GFP in the pK7FWG2 vector (Karimi et al. ...
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bioRxiv - Microbiology 2020Quote: ... coli K-12 ΔhflX strain was complemented by reintroducing hflX ORF using pBAD replicative plasmid (Invitrogen, CAT No.: V44001) E ...
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bioRxiv - Genomics 2020Quote: ... the point mutation was generated in the RFA1 plasmid from the Molecular Barcoded Yeast ORF library from Thermo Fisher Open Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR product which included the entire ORF was cloned into the pENTR/D-TOPO vector (ThermoFisher, Cat#K240020). Using Gateway LR cloning ...
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bioRxiv - Microbiology 2023Quote: ... All ORF sequences were then transferred by in vitro recombination (Gateway™ LR Clonase™ II Enzyme mix, Invitrogen) into a Gateway™ compatible pCI-neo-3xFLAG final destination vector (kind gift from Yves Jacob ...
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bioRxiv - Biochemistry 2024Quote: ... vivax (UniProt entry A0A564ZT73; ORF name PVP01_0807400; 1397 amino acids) were codon optimized for expression in Escherichia coli (ThermoFisher). The gene coding for the AP2-O5 protein of P ...
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bioRxiv - Microbiology 2024Quote: ... located outside of the open reading frame (ORF) based on multiple sequence alignments from rodent PKRs and cloned into the pCR2.1-TOPO-TA vector (Invitrogen) for sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... the full-length ZmbZIP75 ORF was amplified and cloned into the EcoRI/SalI sites of pET-28-SUMO (Invitrogen). The anti-ZmbZIP75 antibodies were produced in rabbits by ABclonal (Wuhan) ...
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bioRxiv - Genetics 2021Quote: Total RNA was extracted from issues of C57BL/6 mice and isolated distal convoluted tubule (DCT) cells of parvalbumin-GFP mice using Trizol Reagent (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... 30 μL of N-methyl-N-trimethylsilyltrifluoroacetamide plus 1% trimethylchlorosilane (MSTFA + 1% TMCS, Thermo Scientific) was added to the MSTFA vials ...
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bioRxiv - Biophysics 2021Quote: ... 5% Fetal Calf Serum Heat Inactivated (FCS HI, Thermo Scientific) and 200μg/mL pennicilin/streptomycin (PS) ...
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bioRxiv - Cell Biology 2021Quote: ... and cloned into pcDNA3.1D/V5-His-TOPO (Thermo Fisher Scientific). MRC-5 cells were transfected with the SOD2-V5 expression construct using Polyethylenimine “MAX” (Polysciences Inc.) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 10% heat-inactivated fetal bovine serum (HI-FBS) (Gibco) at 37°C in the presence of 5% carbon dioxide (CO2) ...
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bioRxiv - Bioengineering 2021Quote: ... The anti-His(C-term)-HRP antibody (R931-25, Invitrogen) was used ...
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bioRxiv - Neuroscience 2020Quote: ... cells resuspended in 500µl 2% HI-FBS + PBS (ThermoFisher 12484028), and passed through a 40µm nylon mesh cell strainer (FisherScientific 352235) ...
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bioRxiv - Genomics 2020Quote: ... for over-expression or pcDNA4/TO/myc-his-B (Thermofisher #V103020 ...
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bioRxiv - Neuroscience 2020Quote: The backbone plasmid pcDNA6/V5-His was purchased from Invitrogen Inc ...
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bioRxiv - Biophysics 2021Quote: ... 5% Fetal Calf Serum Heat Inactivated (FCS HI, Thermo Scientific) and 200μg/mL pennicilin/streptomycin (PS) ...
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bioRxiv - Microbiology 2020Quote: ... Whole cell lysates were added to His beads (Thermo Scientific HisPur Ni-NTA Magnetic Beads ...
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bioRxiv - Biophysics 2020Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS antibody (Thermo Fisher), and 400 nM FITC-labeled TRAP220 or NCoR peptide in a buffer consisting of 20mM potassium phosphate (pH 8) ...
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bioRxiv - Biophysics 2020Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS antibody (Thermo Fisher), and 5 nM Fluormone Pan-PPAR Green fluorescent tracer ligand (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... we first placed ITPRIPL1 protein with 6x-His antibody (Invitrogen) first and slowly rotated at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... dissolved in 12μl of Hi-Di formamide (Thermo fisher, 4311320), denatured at 95°C for 5 mins followed by snapchill ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% heat-inactivated (HI)-FBS (Gibco, 10438-026), 100 ng/mL IL21 (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... All PCR reactions were performed using Hi-Fi Taq (Invitrogen) with appropriate primers.
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bioRxiv - Microbiology 2021Quote: ... 10% heat inactivated fetal bovine serum (HI-FBS; Life Technologies) and 1X penicillin/streptomycin (pen/strep ...
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bioRxiv - Molecular Biology 2022Quote: ... and cloned it in the pcDNA3.1/myc-His(-) plasmid (Invitrogen) in frame with a Myc-His tag to obtain the wild-type (WT ...
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bioRxiv - Molecular Biology 2023Quote: ... with 5% heat inactivated Fetal bovine serum (HI-FBS) (Gibco). 1 million cells per 100 μl per sample were stained with anti-Flag antibody (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... Blue Mix Hi-ROX (PCR Biosystems) and StepOnePlus (Applied Biosystems). The data expressed as the ratio between the expression level of IL-6 mRNA and that of Actin ...
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bioRxiv - Cell Biology 2024Quote: ... The anti-HIS antibody (MA1 21315) was purchased from Invitrogen.
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bioRxiv - Bioengineering 2024Quote: Nitrocellulose transfers were stained with anti-His (Invitrogen PA1-983B), anti-SARS-CoV-S (Absolute Antibodies CR3022) ...
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bioRxiv - Biophysics 2024Quote: ... 7 mL of His-Pur Ni-NTA resin (Thermo Scientific) was equilibrated in Buffer A containing 40 mM imidazole ...
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bioRxiv - Biophysics 2023Quote: ... The medium was supplemented with 10% HI-FBS (Gibco, USA), 2 mM L-glutamine ...
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bioRxiv - Biochemistry 2023Quote: ... bio-His-PADI4 was incubated with magnetic streptavidin beads (Invitrogen) (4 °C ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... The Ion PI Hi-Q Sequencing 200 kit (Life Technologies) was used for all sequencing reactions and Torrent Suite™ Software 5.0 (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were cloned into pcDNA3.1/Myc-His(+) (Invitrogen). Full-length sequences of DBA and 129 IFI207 were obtained from the cloned cDNAs ...
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bioRxiv - Microbiology 2023Quote: ... protein expression from plasmid (derivates of pBAD-His/B (Invitrogen) in all cases except expression of VirF from pCL5 [71] ...
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bioRxiv - Biochemistry 2022Quote: ... 1 nM LanthaScreen Elite Tb-anti-His antibody (ThermoFisher #PV5895), and 400 nM FITC-labeled PGC1α peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Synthetic Biology 2024Quote: ... supplemented with 10% heat-inactivated FBS (HI-FBS, Gibco, 16140071) and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Neuroscience 2024Quote: ... and 10% fetal bovine serum (Gibco FBS HI, Cat# A5256701) in a CO2 incubation at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... The PCR products were cloned into pcDNA3.1/Myc-His(+) (Invitrogen). Full-length sequences of DBA and 129 IFI207 were obtained from the cloned cDNAs ...
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bioRxiv - Neuroscience 2024Quote: ... 10% Value HI fetal bovine serum (FBS) (Thermo Fisher A5256801), 0.1% 2-Mercaptoethanol (Life Technologies 21985023) ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNA encoding full-length murine Sned1 cloned into pCRL-XL-TOPO (clone 40131189) was obtained from Open Biosystems (now, Thermo Fisher, Waltham, MA). Fl-SNED1 and a construct spanning the most N-terminal region of SNED1 and including the NIDO domain (amino acids 1 to 260 ...
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bioRxiv - Microbiology 2021Quote: ... and assembled into full length SARS-CoV-2 cDNA clones in the YAC vector pYESL1 using a GeneArt™ High-Order Genetic Assembly System (A13285, Invitrogen™, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 replicon cDNA fragments were assembled into full-length cDNA clones by TAR assembly using the GeneArt™ High-Order Genetic Assembly System (Invitrogen™, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 replicon cDNA fragments were assembled into full-length replicon cDNA clones by TAR assembly using the GeneArt™ High-Order Genetic Assembly System (Invitrogen™, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... β3 mutants were generated through Quikchange of the IOH3755 cDNA clone and inserted in the pCIG2-GFP-MACF18 plasmid using Gateway cloning (ThermoFisher Scientific; Waltham, MA).
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bioRxiv - Immunology 2021Quote: ... 13-0443-82) and 1:200 VE-cadherin-Alexa Fluor 647(rat anti-mouse, clone 11D4.1, Thermo Fisher, BDB562242) in 1.25% BSA in PBS for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged at 14,000 RPM for 30 seconds and separated on 8% SDS-PAGE gels followed by western blotting with mouse anti-HA (H9658, Clone HA-7, MilliporeSigma) or anti-PGK1 monoclonal antibodies (AB_2532235, 22C5D8, Invitrogen), and secondary polyclonal goat anti-mouse antibodies conjugated to horseradish peroxidase (115–035-146 ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse mAb anti-ZO-1 (clone ZO1-1A12) used in immunofluorescence 1:300 on zebrafish embryos was from Invitrogen. Rat mAb anti-HA High Affinity (clone 3F10) ...