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Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for Glycerol Kinase 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Secondary antibody was diluted to 1:3000 in blocking buffer and added to the plates followed by TMB substrate (Thermofisher Scientific). Upon stopping the reaction with 1M H2SO4 ...
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bioRxiv - Biochemistry 2022Quote: ... 3 times and the following secondary antibodies diluted in blocking buffer were used: Goat anti-rabbit Alexa Fluor 488 (1:500; Thermo Fisher), Cy3-conjugated AffiniPure donkey anti-mouse (1:200 ...
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bioRxiv - Genomics 2022Quote: Samples were then blocked for 1 hour at room temperature in Blocking Solution (1x PBST, 10% Molecular-grade BSA (ThermoFisher AM2616)) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were stained overnight with the following primary antibodies diluted in blocking buffer: mouse anti-neurofilament-M (1:1500, RMO270, Invitrogen, RRID:AB_2315286); rabbit anti-6xHisTag (1:2000 ...
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bioRxiv - Physiology 2022Quote: ... Triton-X in PBS for three minutes followed by blocking with blocking buffer (1% w/v bovine serum albumin with 0.2% w/v keyhole limpet hemocyanin in PBS; Thermo Fisher Scientific) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The primary anti-body was washed away in PBS containing 1% DMSO (wash buffer) and larvae were incubated in blocking solution containing Goat anti-Rabbit546 (ThermoFisher #A11010) at 1:1000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with PBS and incubated with Alexa Fluor-488 conjugated goat anti-mouse IgG secondary antibody diluted at 1:500 in blocking solution (Invitrogen, USA). Fluorescent foci were visually counted (in a well with a dilution that produced <60 total foci ...
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bioRxiv - Physiology 2023Quote: ... cells were permeabilized in 0.25% Triton X-100 and blocked in Sea Block Blocking Buffer (1 h; Thermo Fisher Scientific, USA). Overnight incubation with primary antibodies (anti-VPS36 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 h with secondary antibodies diluted in blocking buffer to 1:600 (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen, Thermo Fisher, A21206 ...
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bioRxiv - Bioengineering 2023Quote: ... To minimize nonspecific binding the samples were blocked for 4 h at room temperature with a blocking buffer containing 1% of Tween20 (Fisher Scientific) and 3 % of BSA (VWR ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated for one hour at room temperature with blocking buffer containing secondary antibodies for Pax7 (ThermoFisher #A-11072; 1:500) and MyoD (ThermoFisher #A-11001) ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibody was diluted in blocking solution and added to cells (30 minutes, room temperature) with phalloidin-AlexaFluor568 (1:100 dilution, Invitrogen, A12380) included when indicated ...
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bioRxiv - Developmental Biology 2022Quote: ... Table 2) incubated for 3 overnights at 4 °C in blocking buffer with Alexa-dye conjugated secondary antibodies (1:500, Molecular Probes) incubated for 1 overnight at 4 °C in blocking buffer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The following day embryos were thoroughly washed with NPBS + 0.1% Triton and incubated first in blocking solution for 3 hours and then overnight at 4°C in the dark in blocking solution with DAPI (1:500) and secondary antibodies: goat anti-mouse (Invitrogen 84540) and goat anti-rabbit (Abcam 150083) ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibody (diluted in the blocking solution) was used: rabbit anti–PGP9.5 (1:400, Thermo Fisher Scientific, #PA5-29012), rabbit anti-S100β (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated in blocking buffer containing secondary antibodies (Alexa Fluor 555 donkey anti-rabbit IgG, 1:500 dilution, A31572, Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... Blocking and permeabilization were performed for 1 h in 1 mL blocking buffer - Dulbecco’s phosphate buffered saline (DPBS) containing 5% (v/v) normal goat serum (Thermo Fisher Scientific) and 0.3% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were incubated in a solution of secondary antibodies diluted in blocking buffer (antibodies and dilutions described in Table 3) and DAPI (1 μg/mL; ThermoFisher Scientific) for 30 minutes at RT in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320, CellBlox monocyte/MAC blocking buffer, Invitrogen #B001T07F01). Cells were stained in an antibody cocktail containing the appropriately titrated antibodies described in Supplementary Table S3 for 20 minutes at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated ON at 4°C with the following primary antibodies in blocking solution: chicken anti-GFP (Invitrogen, 1:1000), rabbit anti-somatostatin (BMA-biomedials ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed four times in 0.1 M PBS with 0.5% Triton X-100 for 5-10 minutes each and then incubated in blocking solution containing 1:400 secondary antibody donkey Polyclonal anti-rabbit Alexa Fluor Plus 488 (A32790, ThermoFisher Scientific) for 2.5 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed three times with PBS and incubated for 1 h at room temperature with secondary antibodies diluted in blocking buffer (Alexa fluor (AF) 488 donkey anti-rabbit (1:500; ThermoFisher Scientific), AF568 donkey-anti-rat (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... Then the sections were incubated with primary antibodies in blocking buffer overnight at 4 °C: AT8 (THERMO SCIENTIFIC, MOUSE,1:100), IBA1(WAKO ...
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated overnight at 4 °C with secondary antibody Alexa 647 donkey-α-rabbit (1:500 in blocking solution; Invitrogen), Alexa 568 donkey-α-mouse (1:500 in blocking solution ...
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bioRxiv - Cancer Biology 2024Quote: ... at room temperature in blocking buffer containing the goat anti-rabbit Alexa Fluor 568 conjugate (1:250, Life Technologies #A-11011) secondary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... ALFA-FAM134s were stained directly using a nanobody against ALFA tag coupled to a Abberior Star 635P fluorophore (FluoTag®-X2 anti-ALFA - AbberiorStar635P, NanoTag Biotechnologies GmbH) diluted 1: 500 in permeabilization/blocking buffer (10% FCS (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... the cells incubated with secondary antibodies in blocking solution for 1h at RT in the dark (anti-chicken Alexa 568, 1:1000, A11041, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... before being incubated with blocking solution (1× PBS with 5% Normal Goat Serum [50-062Z; ThermoFisher Scientific], 0.1% Triton X-100) for 15 min and with primary antibodies solutions for 2 to 3 hours at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated for 72-hours in the same blocking solution with anti-FR (1:500, Invitrogen #A6397, made in rabbit) and either anti-TH (1:5K ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were blocked with PLA blocking buffer and incubated with anti-NOD2 antibody (#MA1-16611, ThermoFisher, 1:500: RRID: AB_568643), anti-α-syn aggregate antibody (#ab209538 ...
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bioRxiv - Neuroscience 2024Quote: The blocking solution for all immunostaining approaches was 1X PBS (no Ca2+ / Mg2+) with 1% normal donkey serum (Fisher Scientific; OB003001), 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed three times with blocking solution for a total of 20 minutes and then incubated with secondary antibody (1:500, Invitrogen, #A31572) for 2h ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting peptides were quantified with the A205 nm protein and peptide methodology using a NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, USA).
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bioRxiv - Cancer Biology 2019Quote: ... Three 15 amino acid peptides (P1, P3 and P4) and one 14 amino acid peptide (P2) were synthesized (Thermo Fisher Scientific, Rockford, IL). The purity of the peptides was >95% and each peptide was modified by N-terminal acetylation and C-terminal amidation to enhance their stability.
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bioRxiv - Biochemistry 2021Quote: Fluorescence signal was measured from biotinylated peptides (biotin-NHS or biotin-SS-NHS) using Quantitative Fluorometric Peptide Assay (Thermo Scientific Pierce, cat.no.: 23290) and following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and the presence of FRα-binding peptide in the nuclear fraction was shown by western blot analysis using the antibody against FRα1-binding peptide (Thermo Fisher, ref: PA5-101588).
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bioRxiv - Biochemistry 2023Quote: ... The pooled TMT-labeled peptide sample was then fractionated using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific 84868). We followed the manufacturer’s instructions except that the percentage of acetonitrile used in the elution buffers and the subsequent concatenation strategy was performed as described below ...
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bioRxiv - Genetics 2024Quote: ... TMT-labeled peptides were fractionated into 8 fractions using the high pH reverse-phase peptide fractionation kit according to manufacturer’s instructions (Thermo Fisher Scientific) (Pierce High pH Reversed-Phase Peptide Fractionation Kit, Thermo Fisher Scientific, #84868) and subsequently dried using vacuum centrifugation.
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bioRxiv - Biophysics 2020Quote: ... lymph-node derived cells were mixed in a 1:2 ratio with OVA-peptide pulsed spleenocytes and cultured in RPMI 1640 culture medium (Gibco) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2020Quote: ... Percolator False Discovery Rate was set to 1% and Peptide abundance values were normalized in Proteome Discover 2.2 (Thermo Scientific). The experiments were performed in two separate sets consisting of (1 ...
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bioRxiv - Neuroscience 2020Quote: ... the peptides from each sample were reacted for 1 h with the a ppropriate TMT-NHS isobaric reagent (Thermo Fisher) in 40% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... coli digests and Promega yeast protein digest were each fractionated by offline reversed-phase LC (Waters Acquity UPLC Peptide BEH300 C18 1.7 µm, 1 mm i.d. x 150 mm column employing an Ultimate 3000 HPLC, Thermo Fisher Scientific) operating with column compartment temperature of 60 °C and flow rate of 100 µL/min ...
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bioRxiv - Bioengineering 2021Quote: Around 1 µg of peptides were run on the pre-column (Acclaim PepMap™ 100, 75μm × 2cm, nanoViper, Thermo Scientific) and the analytical column (Acclaim PepMap™ RSCL ...
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bioRxiv - Biochemistry 2020Quote: ... 1 nM of each acyl-peptide was added to 100 ng of a HeLa tryptic digest standard (Thermo Scientific, #PI88329) for a final ratio of 1 fmol acyl-peptide/100 ng of HeLa digest ...
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bioRxiv - Cell Biology 2021Quote: ... For peptide treatment, cells were washed with PBS and treated with peptides (40 μM, 1–4 h) dissolved in OPTI-MEM (Gibco) acidified with 0.15% (v/v ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of peptides was injected onto a nanoscale C18 reverse-phase chromatography system (UltiMate 3000 RSLC nano, Thermo Scientific) and electrosprayed into an Orbitrap mass spectrometer (LTQ Orbitrap Velos Pro ...
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bioRxiv - Cancer Biology 2022Quote: ... peptides in 1% (v/v) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (ThermoFisher Scientific, Inc). After washing with 0.5% (v/v ...
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bioRxiv - Immunology 2022Quote: ... 106 splenocytes were ex vivo stimulated with SARS-CoV-2 peptide pool (JPT PM-WCPV-S-1) at concentration of 2 μg/ml of each peptide in RPMI (Biowest) supplemented with 10% heat inactivated FBS (Gibco). PMA/Ionomycin (BD Pharmingen™ ...
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bioRxiv - Systems Biology 2022Quote: ... Peptide analyses (corresponding to 1 µg protein) were performed on a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific) connected to an EASY-nLC 1200 ultra-HPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap® C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...