Labshake search
Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for Glycerol Kinase 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2020Quote: ... Slides were then washed with PBS-T (3 × 5 min) and incubated with fluorophore-conjugated secondary antibodies (1:500 in blocking buffer, Invitrogen). Hoechst 33258 (Invitrogen ...
-
bioRxiv - Systems Biology 2019Quote: ... sections were washed with blocking solution and incubated with DAPI (1 μg/ml in PBS, Thermo Fisher Scientific Inc.; D1306) to stain DNA for 10 min at RT before they were covered with coverslips using Fluoromount-G® (SouthernBiotech ...
-
bioRxiv - Neuroscience 2021Quote: ... slices were washed 3 times in PBS and incubated for a further hour at room temperature in diluted blocking solution containing 1:500 goat anti-mouse Alexa488-conjugated IgG secondary antibody (#A28175, RRID:AB_2536161, Invitrogen, Carlsbad, CA) and 1:5000 Hoechst stain (Thermo Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were rinsed 3 times in the blocking solution and incubated in Alexa Fluor 594-conjugated (red) goat secondary antibody (1:500; Invitrogen) for 2 hr.
-
bioRxiv - Microbiology 2022Quote: ... Goat anti-hamster IgG (H+L) HRP conjugated secondary antibody (SeraCare 5220-0371) was diluted 1:3,000 in casein in PBS blocking buffer (Thermo Fisher), and 100 μL was added to the wells and allowed to incubate for one hour at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... Microtubules were then stained using a rat anti-tubulin primary antibody solution (diluted 1:500 in blocking/permeabilization buffer, MA1-80017, Invitrogen) with a 4°C overnight incubation ...
-
bioRxiv - Cancer Biology 2022Quote: ... Sections were rinsed with PBS-TB (PBS supplemented with 0,05% Tween and 1% BSA) and incubated for 30 minutes with 200μl Superblock blocking buffer (Thermo Scientific, #37515). Antibody incubation was split into two steps ...
-
bioRxiv - Cell Biology 2022Quote: ... The following day slides were washed thrice with blocking buffer for 15 min and incubated for 1 h at RT in secondary antibodies and Hoechst 33342 (Invitrogen) diluted in blocking buffer ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were washed in blocking buffer with Hoechst nuclear stain (1:10,000) and mounted with Prolong Gold Antifade (Thermo Fisher).
-
bioRxiv - Neuroscience 2024Quote: ... Brain sections were washed three times for 5 minutes in PBS and incubated for 1 hour in blocking solution [10% normal goat serum (NGS; #50197Z, Invitrogen), 0.1% Triton X-100 in PBS] ...
-
bioRxiv - Developmental Biology 2024Quote: ... the samples were washed extensively in PBS and then incubated overnight at 4°C in blocking solution with the appropriate concentration of secondary antibodies diluted at 1:500 and with DAPI (Invitrogen) diluted at 1:1000 ...
-
bioRxiv - Cell Biology 2024Quote: ... the cells were washed with permeabilization/blocking solution and stained with a 1:400 dilution of Alexa488-conjugated anti-mouse antibody (Invitrogen) for 1h at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were then incubated for 2h at RT in the same blocking solution containing 1:1000 dilutions of corresponding Alexa secondary antibodies (Invitrogen) made in goat and coupled to either 488 ...
-
bioRxiv - Neuroscience 2023Quote: ... they were incubated in primary antibody diluted in blocking buffer using the following primary antibodies: anti-Phospho-Tau (Ser396) (1:500, 44-752G, Invitrogen), anti β-catenin (E-5 ...
-
bioRxiv - Cancer Biology 2022Quote: ... in blocking buffer overnight in 4°C and stained with secondary AlexaFluor633 donkey anti-goat antibodies (Invitrogen, #A21082; 1:200) for 1 hour at RT ...
-
bioRxiv - Biophysics 2022Quote: ... the tissues were washed with blocking solution three times and incubated with the respective secondary antibody (polyclonal goat anti-mouse IgG1, 1:500, Invitrogen) for 45 min at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... the pellet was resuspended in 90 μl of Secondary antibody solution (Blocking buffer + 1:500 Goat anti-rabbit IgG AlexaFluor 647 antibody [#A27040, Invitrogen]) and the samples were incubated at room temperature for 45 min in the dark ...
-
bioRxiv - Neuroscience 2023Quote: ... The sample was washed in PBT for 3 hr at 25 °C then incubated in the secondary antibody AF488-conjugated goat-anti-mouse (1:500 in blocking buffer, Ref# A21202, Invitrogen) at 4 °C for 24 hr ...
-
bioRxiv - Neuroscience 2023Quote: ... in PBT then incubated for 24–48 hr at 4 °C in the primary antibody mouse anti-GFP (1:500 in blocking buffer, Ref# A11120, Invitrogen). The sample was washed in PBT for 3 hr at 25 °C then incubated in the secondary antibody AF488-conjugated goat-anti-mouse (1:500 in blocking buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... the sections were incubated in a biotinylated goat anti-rabbit secondary antibody in blocking solution (Invitrogen, cat # 31820, 1:500) and transferred in a detection reagent (Vectastain Elite ABC kit ...
-
bioRxiv - Developmental Biology 2023Quote: ... 200 μl of blocking buffer containing a 1:1000 dilution of goat anti-rabbit Alexa 488 (Thermo Fisher; catalog #R37116) was added to the well and samples were incubated overnight on a rocking incubator at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... retinas were incubated on a rocking platform for 2 hours at room temperature (RT) in Claudio’s blocking buffer (CBB) consisting of 1% FBS (LTID 10500-064, Thermo Fisher), 3% BSA (MB04602 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and probed with an Alexa Fluor 488-conjugated goat-anti-rabbit secondary antibody diluted in blocking buffer (1:100, #A11008, Invitrogen) for 1 h at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... The slides were then rinsed 4 times with the washing buffer for 15 minutes and incubated with the secondary antibody at 1:500 dilution in blocking solution for 1h at room temperature (Rabbit 555, Invitrogen, #AB_162543 ...
-
bioRxiv - Neuroscience 2023Quote: ... the sections were transferred to blocking solution containing 1:1000 primary antibody rabbit Polyclonal anti-GFAP (PA5-16291, ThermoFisher Scientific) for 24 hours at 4 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 h with secondary antibodies diluted in blocking buffer to 1:600 (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen, Thermo Fisher ...
-
bioRxiv - Neuroscience 2022Quote: ... the sections were incubated in a biotinylated goat anti-rabbit secondary antibody in blocking solution (Invitrogen, cat # 31820, 1:500) and transferred in a detection reagent (Vecstatin Elite ABC kit ...
-
bioRxiv - Microbiology 2023Quote: ... Monolayers were washed three times with blocking buffer before incubation in the dark with a 1:1000 dilution of Alexa546-conjugated anti-rabbit IgG (ThermoFisher) for 1 hour at 23ºC ...
-
bioRxiv - Neuroscience 2023Quote: ... Sections were then washed twice in 0.01 M PBS for 10 min followed by a 1 hr incubation in a blocking solution comprised of 0.3% Triton X-100 (Fisher Scientific) and 1% Bovine Serum Albumin (BSA ...
-
bioRxiv - Developmental Biology 2023Quote: ... at RT for 10 min and blocked at RT for 1 hr in blocking solution (5% NBCS, 2x antibiotic-antimycotic (Gibco), 0.1% sodium azide (Sigma s2002-100G) ...
-
bioRxiv - Microbiology 2023Quote: ... the membranes were incubated with an anti-TasA antibody (rabbit) at a 1:20,000 dilution in Pierce Protein-Free (TBS) blocking buffer (Thermo Fisher). A secondary anti-rabbit IgG antibody conjugated to horseradish peroxidase (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... followed and the slides were incubated with secondary antibody (1:400 in blocking buffer, donkey a-rabbit Alexa 488 antibody, Invitrogen A-21206 ...
-
bioRxiv - Neuroscience 2023Quote: ... Neurons were incubated in secondary antibodies in blocking buffer for 1 hour at room temperature in the dark (Alexa Fluor 488 goat anti-chicken, Invitrogen A-11006 ...
-
bioRxiv - Immunology 2023Quote: ... Cells were washed with PBS and incubated in blocking buffer for 1 h with Streptavidin-AlexaFluor 488 (S11223, Molecular Probes), goat anti-Mouse IgG-AlexaFluor 555 (A-21424) ...
-
bioRxiv - Cell Biology 2023Quote: ... Primary antibodies rabbit anti-HA (1:500) was subsequently added in permeabilization/blocking buffer (10% fetal calf serum (FCS) (Gibco), 0.1% saponin (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Followed by two more PBS rinses prior to 1 hour incubation with SuperBlock™ Blocking Buffer (Thermo Scientific™,37515) for blocking non-specific antibody binding ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed 1-2 times with 1X PBS and incubated for 2 hours in Alexa Fluor conjugated goat anti-rabbit/ chicken or donkey anti-goat/rabbit secondary antibodies along with DAPI (dilution 1:1000 X in blocking buffer) (Invitrogen) at room temperature ...
-
bioRxiv - Developmental Biology 2024Quote: ... incubated 1 hour in blocking solution and incubated at 4 °C overnight with secondary antibodies conjugated with AlexaFluor (Molecular probes) diluted in blocking solution ...
-
bioRxiv - Physiology 2024Quote: ... Cells were then immunostained using CDH5 antibody [36] diluted 1:100 in blocking buffer for 30 min followed by Alexa Fluor 555–conjugated goat anti–rabbit antibodies (ThermoFisher) diluted 1:200 in blocking buffer together with Hoechst ...
-
bioRxiv - Cancer Biology 2024Quote: ... We then incubated membranes with secondary antibody (in 5% Blocking Buffer) for 1 hour at room temperature (Invitrogen cat. A16110; Invitrogen cat ...
-
bioRxiv - Cell Biology 2024Quote: ... Microtubules were then stained using the rat anti-tubulin primary antibody (1:500 in blocking/permeabilization buffer, MA1-80017, Invitrogen) with a 4 °C overnight incubation ...
-
bioRxiv - Neuroscience 2024Quote: ... and revealed with appropriate Alexa fluorophore-conjugated secondary antibodies (1:1000 in blocking buffer, A11013 and A21429, Thermofisher, Courtaboeuf, France) and DAPI (10 µg/mL ...
-
bioRxiv - Genomics 2024Quote: ... and pellets were resuspended in ice-cold bovine serum albumin (BSA) blocking solution (1× phosphate-buffered saline (PBS) (AM9625, Ambion), 0.5% BSA (VWR ...
-
bioRxiv - Plant Biology 2019Quote: ... Phospho-peptides were enriched from the remaining sample (900 µg) using High-Select Fe-NTA Phospho-peptide Enrichment Kit (A32992, Thermo Fisher Scientific). The TMT labeled samples were analyzed on a Fusion Lumos mass spectrometer (Thermo Fisher Scientific) ...
-
bioRxiv - Systems Biology 2019Quote: ... Lymphocyte samples were prepared as 50 ng/µL total sample peptide concentration with Pierce Peptide Retention Time Calibration Mixture (PRTC, Thermo Fisher Scientific) spiked in at a final concentration of 2 fmol/µL and queued in blocked and randomized order with two technical replicates analyzed per sample ...
-
bioRxiv - Biochemistry 2022Quote: ... peptides were fractionated into a final eight fractions using a Pierce High pH reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific, 84868), according to the manufacturer’s instructions.
-
bioRxiv - Genomics 2022Quote: ... A portion of the peptides from the samples were pooled and fractionated using a Pierce High pH Reversed-Phase (HPRP) Peptide Fractionation Kit (Thermo Fisher Scientific) to generate a spectral library ...
-
bioRxiv - Microbiology 2022Quote: Peptide concentrations were measured for fraction two of each sample using the Quantitative Fluorometric Peptide Assay kit (Pierce™ Thermo Fisher Scientific) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2020Quote: ... An aliquot of the tryptic digests was used to determine the concentration of the peptide mixtures by using Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... The flow-through containing endogenous peptides was collected and peptide concentration was measured using a bicinchoninic acid (BCA) protein assay (Thermo Scientific Pierce) as per manufacturer’s protocol ...