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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Glycerol Kinase 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 10 µM Rho-kinase inhibitor Y-27632 and 2% KnockOut™ SR XenoFree Medium Supplement (Gibco) were added to the medium for the first day ...
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bioRxiv - Cancer Biology 2024Quote: ... beads were incubated in kinase reaction buffer supplemented with 100 ng recombinant CAMK2D (Invitrogen, cat. PV3373), 100 µM recombinant calmodulin (Enzo ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10% glycerol) using Slide-A-Lyzer Dialysis Cassettes (MWCO 10 kDa, Thermo Fisher Scientific) or Amicon Ultra (MWCO 10 kDa ...
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bioRxiv - Molecular Biology 2021Quote: ... the slide was mounted with 80% glycerol containing Hoechst 33342 (10 µg/ml, Invitrogen, H3570).
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bioRxiv - Neuroscience 2019Quote: ... Brains were soaked in 50% glycerol in PBS or SlowFade Gold antifade reagent (Invitrogen S36936) for 20 min before mounting.
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bioRxiv - Biochemistry 2021Quote: ... 5% (v/v) glycerol) supplemented with a protease inhibitor cocktail (EDTA-free, Thermo Scientific Pierce) and mechanically lysed using a homogeniser ...
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bioRxiv - Neuroscience 2021Quote: ... The final buffer for the virus was: dPBS + 5% Glycerol + 0.001 Pluronic F-68 (Invitrogen). The AAV titre (1.71 × 1013 GC/ml ...
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bioRxiv - Cell Biology 2020Quote: ... 10% glycerol) before being supplemented with NuPAGE LDS sample buffer (Life Technologies, Carlsbad, CA, USA) and loaded on 4-12% NuPAGE gels (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... 50% glycerol) and run on a Novex NativePAGE 4 to 16% Bis-Tris gel (Invitrogen). Separated proteins were transferred to a polyvinyl difluoride membrane and detected via either ...
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bioRxiv - Molecular Biology 2021Quote: ... 10% glycerol) were loaded on 3-12% gradient Bis-Tris NativePAGETM precast protein gels (Invitrogen) and subjected to BN- PAGE electrophoresis as described previously (75 ...
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bioRxiv - Biophysics 2019Quote: ... 50% (w/v) glycerol) and subjected to 4%-16% blue native PAGE mini gel (Invitrogen) for electrophoresis at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... and 10% glycerol) in the presence of a protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific). The suspension was incubated for 30 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... cells were applied with DMEM containing 10 % glycerol as a cryo-protectant and Dynabeads (Invitrogen) at 1:40 dilution for 3D CLEM workflow ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% sodium deoxycholate and 10% glycerol) supplemented with Complete protease inhibitor cocktail (Thermo Fisher Scientific). Protein content was quantified by Bradford assay54 and 20 μg of protein of each cell line were separated by SDS-PAGE and electrotransferred to PVDF membranes (Millipore Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... Fish larvae were cleared in 80% glycerol and mounted in custom-made coverslips (Fisher Scientific). Meckel’s length ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Thawed glycerol stocks were replicated from 96-well plates to omnitrays (Nunc, Rochester, NY, USA) in 96-array format on YPD agar medium ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol and 500 mM NaCl supplemented with EDTA-free protease inhibitor (Thermo Fisher Scientific). The cells were lysed using an Avestin Emulsiflex C3 homogeniser ...
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bioRxiv - Microbiology 2023Quote: ... Tm glycerol stocks were pinned onto selective rectangular LB-agar plates (Thermo Fisher Scientific #267060) containing 20 µg/mL Cm and 50 µg/mL Strep ...
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bioRxiv - Cell Biology 2023Quote: ... 48 hours after transfection cell lysates were prepared by adding lysis buffer (10% Glycerol (Invitrogen), 20mM Tris-HCL ...
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bioRxiv - Microbiology 2024Quote: ... 100μl of culture was added to 100μl of 40% glycerol solution (Thermo Fisher Scientific, 17904) in purified water ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and then incubated in the dark for 1 h with secondary antibodies in 200 μl of blocking buffer (polyclonal goat anti-mouse Alexa Fluor® 488, 1:200 (A32723, ThermoFisher) and goat anti-rabbit Alexa Fluor® 647 ...
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bioRxiv - Cell Biology 2020Quote: ... and treated with donkey Alexa Fluor 488-conjugated anti-rabbit IgG secondary antibody (A21206, Life Technologies; 1:1000 in blocking buffer) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... They then had further blocking solution added to them containing Alexa Fluor 488/568 secondary antibodies (1:1000; Thermo Fisher Scientific) in addition to DAPI (1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed in TBS-T and incubated for 1 h at RT with a mixture of the following secondary antibodies diluted 1:200 in blocking solution: anti-guinea pig Alexa Fluor 488 (Life Technologies) and anti-rabbit Alexa Fluor 568 (Life Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... The following secondary antibodies were used at 1:1000 dilution in blocking solution: Alexa Fluor 647 anti-Rabbit (Thermo Fisher, #A21244), Alexa Fluor 647 anti-Goat (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... A secondary antibody mixture was made using the same permeabilization/blocking solution with 1:1000 dilution of anti-mouse Alexa Flour 488 (Thermo Fisher) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... then incubated in blocking buffer composed of 1×PBS supplemented with 10% Gibco Fetal Bovine Serum (Thermo Fisher Scientific, Cat. A4766801) and 0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated overnight (4°C) in the same blocking solution containing the primary rabbit anti-PV antibody (1:1000; Thermo Scientific) and mouse anti-SST antibody (1:250 ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed in TBS-T buffer and then incubated for an hour at room temperature with the corresponding horseradish peroxidase-conjugated preadsorbed secondary antibody (1/2000, blocking solution corresponding, Molecular Probes). Membranes were washed in TBS-T buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then rinsed again in buffer solution and then immersed in secondary antibody solution (blocking solution and 1:500 donkey anti-mouse IgG conjugated with Alexa Fluor 488 (Thermo Fisher Scientific Cat# A-21202 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed in TBS-T buffer and then incubated for an hour at room temperature with the corresponding horseradish peroxidase-conjugated pre-adsorbed secondary antibody (1:5000, blocking solution corresponding, Molecular Probes). Membranes were washed in TBS-T buffer and immunoreactive bands were visualized with the SuperSignal West Pico Chemiluminescent Substrate (Pierce) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in blocking solution for 2 h at room temperature with fluorescent donkey anti-goat alexa fluor 488 (1:500, Invitrogen, A11055) and goat anti-rabbit alexa fluor 647 (1:500 ...
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bioRxiv - Systems Biology 2021Quote: ... with an estimated each concentration of 1-5 ng/μl were incubated in the blocking solution with 100-fold diluted SUPERase In RNase Inhibitor (Invitrogen AM2694) at room temperature for 18-24 hours ...
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bioRxiv - Neuroscience 2020Quote: ... then placed overnight at 4 0C in rabbit anti-GFP primary antibody (1:1000 in blocking solution, Invitrogen, La Jolla, CA). Following incubation ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 1 h (at RT with gentle shaking) in a blocking solution containing 10% normal goat serum (16210064, Life Technologies), 0.4% Triton X-100 and 0.1% glycine in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed in blocking solution three times and incubated with goat anti-rat Alexa 488 secondary antibody (1:500 dilution, Molecular probes) at room temperature for 1 h ...
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bioRxiv - Systems Biology 2020Quote: ... The following primary antibodies were diluted in the blocking solution and applied overnight at 4°C: mouse anti-TAU clone HT7 (1:500, Thermo Scientific), chicken anti-MAP2 (1:10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were again washed with Blocking Buffer twice for 1 h and mounted on microscope slides using ProLong Glass (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... The cells were then incubated for 45 min with secondary antibody diluted in blocking buffer (1:500, Alexa Fluor 568, Molecular Probes) and washed twice with blocking buffer and once with PBS ...
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bioRxiv - Synthetic Biology 2022Quote: ... the gel was transferred to a 0.2 µm nitrocellulose membrane and blocked for 1 h at room temperature with SuperBlock™ blocking buffer (Thermo Scientific). Membranes were then washed four times in TBST buffer before incubation in a 1:5000 dilution of Monoclonal ANTI-FLAG® antibody (Monoclonal ANTI-FLAG® M2-Peroxidase (HRP ...
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bioRxiv - Physiology 2022Quote: ... Cells were then washed with PBS-S-T and incubated for 1h at 37°C with the following secondary antibodies diluted in blocking buffer: goat anti-rat Alexa Fluor 488 (1:500, catalog no. A-11006, ThermoFisher Scientific), goat anti-rabbit Alexa Fluor 594 (1:500 ...
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bioRxiv - Physiology 2022Quote: ... Slides were washed three times for 5 min with TBS + 0.1% Tween 20 and then incubated for 1h at RT with the following secondary antibodies diluted in blocking buffer: goat anti-rat Alexa Fluor 488 (1:500, catalog no. A-11006, ThermoFisher Scientific), goat anti-rabbit Alexa Fluor 594 (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed with PBS three times and incubated in the blocking buffer containing anti-mouse IgG donkey antibody conjugated with Alexa 488 (1:500 dilution, Thermo Fisher), anti-rabbit IgG donkey antibody conjugated with Alexa 555 (1:500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary antibodies were Alexa Fluor 488 goat anti-mouse (green) and Alexa Fluor 568 goat anti-rat (red) (used at 1:250 dilution in blocking solution, Life Technologies).
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bioRxiv - Biophysics 2020Quote: HEK293 cells stably expressing TfR-GFP were plated on fibronectin coated MatTek dishes and the next day incubated with 1 μM Alexa 647 labelled AP3 aptamer in blocking buffer (2 mM salmon sperm DNA, ThermoFisher #15632011), 2 mM yeast tRNA (ThermoFisher #AM7119 ...
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bioRxiv - Neuroscience 2022Quote: ... The treated cells were then incubated with blocking buffer at room temperature for 1 hour and primary antibodies (anti-NeuN (NC1284461, Fisher Scientific)) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and permeabilized with cold methanol at −20°C for 2 min or 0.1% TX-100 in PBS for 5 min at room temperature followed by blocking with 0.12% glycine (Fisher Scientific, G46-1) in PBS for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... three times and then blocking buffer (5% normal goat serum [Cat #PCN5000, ThermoFisher], 0.2% Triton™ X-100 [Cat #9002-93-1, Fisher Scientific] ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 (Invitrogen, Carlsbad, CA; 1:500).
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were washed with 1x PBS and secondary antibodies diluted in blocking solution were added 4 hrs at RT: anti-Rabbit 555 (1:800; Invitrogen; A31572), anti-Chicken Cy2 (1:800 ...