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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Relative gene expression was determined by quantitative real time PCR (q-RT-PCR) using the ViiA 7 Real Time PCR system (Applied Biosystems, USA). The reaction mixture consisted of 0.75μL 500nM forward primer ...
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bioRxiv - Immunology 2021Quote: RNA samples were quantified by quantitative reverse transcription-PCR (qPT-PCR) using Superscript III one step RT-PCR system with Platinum Tag Polymerase (Invitrogen, Darmstadt, Germany). Primer and probe sequences for mRNA encoding the SARS-CoV-2 E gene (Corman et al. ...
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bioRxiv - Immunology 2022Quote: Real-time RT-PCR was performed using the ViiATM 7 Real-Time PCR System and Power SYBRTM Green PCR Master Mix (ThermoFisher Scientific #4368708). Primers for HA (forward 5’-AAACTCTTCGCGGTCTTTCCA-3’ ...
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bioRxiv - Cell Biology 2019Quote: Analysis was performed using the Applied Biosystems validated TaqMan primer-probe set (Hs00208333/ spans exon 6 and 7) and the ABI PRISM SDS7000 sequence detection system (Applied Biosystems). The ΔCT method was used to quantify relative mRNA levels as described in ABI user guide ...
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bioRxiv - Cell Biology 2020Quote: ... using commercial buffer and primer sets for the amplification of ACE2 (Hs01085333m1) and TMPRSS2 (Hs01122322m1) transcripts (Thermo Fisher Scientific, Darmstadt, Germany). We analysed the transcript of TATA box binding protein (TBP ...
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bioRxiv - Evolutionary Biology 2022Quote: The amplified fragments obtained with the Arsenophonus primer set were sequenced with the BigDye™ Terminator v1.1 Cycle Sequencing Kit (ThermoFisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Syber green-based qPCR was performed using primer sets (Table S1) with 50 ng of cDNA from each sample in the QuanStudio-6 qPCR system (Applied Biosystems) with thermocycle condition ...
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bioRxiv - Cell Biology 2022Quote: ... with gene-specific primer sets (Supplemental Table 2) using the Platinum SYBR Green qPCR SuperMix-UDG with ROX (Invitrogen, #11733-046) under the following reaction conditions ...
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bioRxiv - Microbiology 2023Quote: ... Both primer sets were used at a final concentration of 0.2 µM with Phusion High-Fidelity DNA polymerase (Thermo Scientific, USA). According to Illumina (San Diego ...
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bioRxiv - Microbiology 2023Quote: ... Viral genome levels were quantified by qPCR using virus specific primer probe sets (described above) and normalised to GAPDH using an Applied Biosystems FAM/MGB probe mix (Thermofisher, 4333764F).
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bioRxiv - Cell Biology 2023Quote: ... Technical triplicate reactions were set up for each sample and target primer in MicroAmp Optical 384-well Reaction Plates (Life Technologies), and reactions were run and analyzed on a Quant Studio 12K Flex Real-Time PCR system (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... gene for the internal normalization primers-probe (VIC) set (VIC-MGB: assay ID Hs03405707_g1; TaqMan Gene Expression Assay, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with miR-1253 Pre-miR miRNA Precursor (Assay ID #PM13220) or scrambled Pre-miR miRNA Precursor negative control #1 (Catalog #AM17110) (ThermoFisher, Waltham, MA). Mimics were transfected with Lipofectamine 2000 (ThermoFisher).
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bioRxiv - Cancer Biology 2020Quote: ... Capan-1 and Hs 766T were transfected with 100 nM Pre-miR Precursor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218 (Ambion ...
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bioRxiv - Physiology 2020Quote: ... as well as non-specific negative control oligonucleotides (5′-AAATGTACTGCGCGTGGAGAC-3′) were cloned into pcDNA6.2-GW/EmGFP-miR [“BLOCK-iT™ PolII miR RNAi Expression Vector Kit” (Invitrogen, Darmstadt, DEU). Human Flag- and myc-tagged GPT and GPT2 cDNA was obtained from Origene (RC203756 and RC209119) ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was performed on QuantStudio™ 3 and 5 Real-Time PCR System (Thermo Fisher Scientific, USA) using KAPA SYBR®Fast qPCR Master Mix (Kapa Biosystems).
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription quantitative PCR (RT-qPCR) was performed using a QuantStudio 3 realtime PCR system (Thermo Fisher Scientific) with SYBR Green JumpStart Taq ReadyMix (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Q-PCR amplifications were performed on a Applied Biosystems Step One Plus RT PCR system (Thermo Fisher Scientific) at 95 °C for 10min ...
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bioRxiv - Cell Biology 2019Quote: ... The q-RT-PCR reactions were run on the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). qPCR results were analyzed using the ΔΔCt method relative to the mean of housekeeping genes (Rps12 ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using a TaqMan One-Step RT-PCR Master Mix Reagents Kit (ThermoFisher, cat. # 4309169) and primers for a genomic locus (b-actin ...
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S-nitrosoglutathione reductase deficiency causes aberrant placental S-nitrosylation and preeclampsiabioRxiv - Physiology 2021Quote: ... The quantitative RT-PCR for indicated genes was performed in TaqMan Universal PCR Master Mix (Applied Biosystems, USA). Quantitation of mRNAs was performed using Applied Biosystems™ TaqMan™ Gene Expression Assays according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The RT-PCR was performed using first-strand cDNA with TaqMan Fast Universal PCR Master Mix (ThermoFisher Scientific). TaqMan primer-probes were obtained from ThermoFisher Scientific (BSG Hs00936295_m1 ...
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bioRxiv - Neuroscience 2020Quote: ... Real-time RT-PCR was performed with the EXPRESSS One-Step SuperScript qRT-PCR kit (Thermo Fisher Scientific) using gene-specific primers (Supplementary Table 2 ...
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bioRxiv - Pathology 2021Quote: ... The PCR mastermix was prepared using the AgPath-ID One-step RT-PCR kit (Thermo Fisher Scientific, USA) in a volume of 10 µl including 0.5 µl of β-Actin-mix2-HEX as internal control and 2.5 µl of extracted RNA ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time RT-PCR (qRT-PCR) was performed using 0.01-0.05μg cDNA with SYBR Green mastermix (Applied Biosystems) in an Applied Biosystems thermocycler ...
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bioRxiv - Genetics 2021Quote: ... RT-PCR was then performed using Phusion Green Hot Start II High-Fidelity PCR Master Mix (Thermo Scientific) and 20-100 ng cDNA ...
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bioRxiv - Microbiology 2020Quote: ... reverse transcription PCR was performed on extracted RNA using SuperScript™ IV One-step RT-PCR System (Invitrogen). The sequence-specific primers were used to generate an amplicon of 905 bp covering the D614G region ...
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bioRxiv - Immunology 2022Quote: ... and analyzed by RT-PCR with a Step One Real-time PCR system as described above (Applied Biosystems).
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bioRxiv - Molecular Biology 2020Quote: ... The RT-PCR was performed using a thermo cycler (ABI 7500 Real-Time PCR, Thermo Fisher Scientific, USA) at 95 °C for 10 min ...
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bioRxiv - Physiology 2020Quote: ... Gene expression was performed by quantitative real time RT-PCR using Power SYBR Green PCR Mix (Applied Biosystems) using StepOnePlus detection system (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Real-time RT-PCR experiments were performed using a Power SYBR™ Green PCR Master Mix (Thermo Fisher). The following primers were used for the qPCR reactions ...
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bioRxiv - Immunology 2022Quote: ... Quantitative PCR was performed with the TaqPath™ COVID□19 CE□IVD RT□PCR Kit (Thermo Fisher Scientific) to obtain mean cycle threshold (Ct ...
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bioRxiv - Genetics 2023Quote: ... Quantitative RT-PCR reactions were carried out on a Real-time PCR machine (QuantStudio 5 by Applied Biosystems), according to a protocol from the Bimake SYBY Green Master Mix qPCR Kit (B21402) ...
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bioRxiv - Cancer Biology 2023Quote: ... Both cDNA synthesis and PCR amplification were done with the SuperScript III one-step RT-PCR system (Invitrogen). TaqMan probes for the housekeeping genes ACTB (Hs01060665_g1 ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR was performed in 48-well plates using the Step-One Real-Time PCR System (Applied Biosystems). Relative changes in gene expression between control and treated samples were determined using the 2 (-Delta Delta CT ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Real-time quantitative RT-PCR (Q-PCR) was performed in a QuantStudio 7/12k Flex System (Applied Biosystems) with the following primers Crbn.F 5’-TGAAATGGAAGTTGAAGACCAAGATAG-3 ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR (RT-PCR) were performed on a QuantStudio 3 or QuantStudio 5 machines (Thermo Fisher Scientific). Primers/probe sets from Applied Biosystems were selected from the Thermo Fisher Scientific website (Supplementary Table 3) ...
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bioRxiv - Developmental Biology 2023Quote: ... Real time (RT)-PCR was performed using Fast SYBR Green PCR Master Mix reagent (Thermo Fisher Scientific, USA) with the following reverse and forward primers ...
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bioRxiv - Cancer Biology 2023Quote: ... The FCM antibody panel and gating strategy are detailed in Supplemental Figure 1.33 CD81 mRNA was quantified by Taqman RT-PCR and GAPDH normalized (CD81: Hs00174717_m1, GAPDH: Hs03929097_g1, StepOnePlus Real-Time PCR System, ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: ... Thermo Fisher) and the Applied BiosystemsTM ViiA Real-Time PCR Systems by relative quantitative RT-PCR (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... and cDNA synthesis and PCR were performed using SuperScript IV One-Step RT-PCR System (Thermo Fisher Scientific) with primers designed at 3’UTR of bovine ZDBF2 according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... these reactions were monitored by real-time PCR on a StepOne RT-PCR instrument (Applied Biosystems, Waltham, MA) and terminated during mid-log amplification (17 or 18 cycles) ...
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bioRxiv - Neuroscience 2019Quote: ... 100 ng of RNA was reverse transcribed using random primers and SuperScript II RT-polymerase (Invitrogen, Carlsbad, CA). Primers were designed using the Primer Express oligo design program software (Applied Biosystems ...
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bioRxiv - Systems Biology 2021Quote: Barcode-drug mixtures for the valve module were prepared by diluting barcoded RT primers in FreeStyle media (ThermoFisher) to 1 µM ...
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bioRxiv - Immunology 2022Quote: ... Total cDNA was generated from 1-2 µg of RNA using random hexamer primers and Revertaid RT (Thermofisher). Taqman qPCR was used to quantify LT mRNA levels with normalization to TATA-Box Binding protein and compared to a standard curve to determine copy number (Maru et al. ...
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bioRxiv - Microbiology 2023Quote: cDNA was prepared from 2 μg RNA using random hexamer primers and SuperScript IV Reverse Transcriptase (RT; Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... mirVana miR inhibitor negative control #1 (Cat 4464076, Ambion), hsa-miR-494-3p inhibitor (MIMAT002816 ...
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bioRxiv - Synthetic Biology 2020Quote: ... miR-31 inhibitor (Invitrogen™ mirVana™ miRNA Inhibitors) was cotransfected using the same method as for DNA (Fig ...
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bioRxiv - Neuroscience 2022Quote: ... hsa-miR-10 (Applied Biosystems miRNA assay ID 000387), hsa-miR-129 (Applied Biosystems miRNA assay ID 000590 ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells transfected using scrambled sequence anti-miR (Ambion, USA) served as a negative control ...