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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Collagen I monoclonal antibody (Invitrogen, MA1-26771), Collagen IV polyclonal antibody (Assay Biotech ...
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bioRxiv - Cell Biology 2021Quote: ... anti-collagen I antibody (PA5-29569; Invitrogen) and anti-cyclophilin (AB178397 ...
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bioRxiv - Microbiology 2021Quote: ... pre-coated with collagen (Thermo Fisher #A1064401) according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Acid-extracted rat tail collagen (Gibco, A1048301) was neutralized on ice in the presence of 10X DMEM with 0.1N NaOH such that the final concentration of the collagen is 1 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Bovine collagen I (1mg/ml, Life Technologies) was then added and live cell imaging was performed for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... and collagen I (ThermoFisher; 5 µg/mL) coated plates ...
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bioRxiv - Bioengineering 2022Quote: ... or collagen X (14-9771-82; Invitrogen) then visualized using a mouse and rabbit specific HRP/DAB (ABC ...
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bioRxiv - Cell Biology 2022Quote: ... DQ Collagen was purchased from Thermo Fisher Scientific (D12060) ...
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bioRxiv - Bioengineering 2024Quote: ... were coated with collagen (Gibco, #A10483-01) and stored at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... S2 cells grown in SFX medium were co-transfected by 3xFLAG-Pita (wild-type and with deletion of CP190-interacting region) and CP190 plasmids with Cellfectin II (Life Technologies), as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... S2 cells grown in SFX medium were co-transfected by 3xHA-dCTCF (wild-type and with deletion of CP190-interacting region) and CP190 plasmids with Cellfectin II (Life Technologies), as recommended by the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: ... DRGs were enzymatically dissociated at 37°C for 2 × 20min in the HBSS solution supplemented with calcium (CaCl2 5 mM) and containing collagenase type II (Gibco, France) and dispase (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... Isolation of cells was performed either by enzymatic digestion using 3 mg/ml collagenase type II (Gibco Life technologies, Vienna, Austria) for 6-8 hours or migration from explants (explants were removed after 7-10 days ...
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bioRxiv - Molecular Biology 2021Quote: ... Isolation of cells was performed either by enzymatic digestion using 3 mg/ml collagenase type II (Gibco Life technologies, Vienna, Austria) for 6-8 hours or migration from explants (explants were removed after 7-10 days ...
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bioRxiv - Cell Biology 2023Quote: HeLa wild-type or HeLa POLE4 KO cells were grown in 8-well Lab-Tek II chambered coverglass 30 (Thermo Scientific) and transfected 48 h prior to imaging with GFP-tagged PARP1 chromobody (Chromotek) ...
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bioRxiv - Biochemistry 2020Quote: ... Fluorescently tagged secondary antibodies were from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Alexa 488-tagged phalloidin (ThermoFisher, 400x stock) was added at 4x working concentration and incubated for 2 days ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG-tagged Fzd4 in pcDNA3.1 (Thermo Fisher), or both Tspan12 and Fzd4 (300 ng each receptor per mL of cells) ...
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bioRxiv - Developmental Biology 2023Quote: ... Appropriate Alexa Fluor-tagged secondary antibodies (Invitrogen) were matched to the primaries and incubated for 2 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Type IV (ThermoFisher) for 1 hr and dissociation into smaller pieces through manual tritulation before seeding onto feeder layer of MEFs.
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg/ml dispase II (Thermo Fisher Scientific), and 2.5% fetal bovine serum (Hyclone ...
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bioRxiv - Immunology 2023Quote: ... or 1-well (Nunc Lab-Tek II 155360) glass-bottom imaging chambers ...
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bioRxiv - Pathology 2023Quote: ... containing 1-mg/ml collagenase II (Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... NL63-CoV and the N-terminal peptidase domain of wild-type and human ACE2 SNVs were expressed using the Bac-to-Bac baculovirus system (Invitrogen). Specifically ...
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bioRxiv - Molecular Biology 2022Quote: Wild type and MED14-dTAG human colorectal carcinoma HCT-116 cells [25] were grown in RPMI 1640 medium (Gibco, 21875034) supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells were infected with the SARS-CoV-2 wild type (isolate SARS-CoV-2/human/SWE/01/2020; Genbank accession: MT093571) in VP-SFM (Gibco). The virus was harvested at day three ...
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bioRxiv - Molecular Biology 2019Quote: ... and 2) 1 U/μL RNaseOUT™ Recombinant Ribonuclease Inhibitor (ThermoFisher Scientific). Hydra polyps in MCB buffer were homogenized on ice using a Dounce homogenizer ...
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bioRxiv - Immunology 2020Quote: Recombinant HIV-1 Env gp120 was expressed in Freestyle 293 cells (ThermoFisher) by transient transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... along with 1 unit of RNaseOUT Recombinant RNase Inhibitor (Invitrogen Cat. 10777019) and 1 mM MgCl2 (Thermo Fisher Scientific Cat ...
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bioRxiv - Cell Biology 2022Quote: ... anti-HA(C29F4, Cell Signalling Technology,WB,1:1000, IF,1:500),anti-His (MA1-21315, Invitrogen; WB,1:10,000), anti-EEA1 antibody was a kind gift from Prof ...
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bioRxiv - Immunology 2022Quote: ... incubated with DPBS containing 10% HI FBS and 1:1000 Hoechst 33342 (Invitrogen, #H3570) for nuclear staining and mounted onto microscope cover slips with Fluoromount G (Southern Biotech ...
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bioRxiv - Microbiology 2019Quote: ... 6×His-Lsr2 was purified by binding to 1 mL Ni-NTA agarose (Invitrogen), after which the resin was collected and the bound protein was washed with binding buffer supplemented with increasing concentrations of imidazole ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit monoclonal anti-6x-His tag at 1:1,000 (Thermo Fisher Scientific, MA5-33032), and mouse monoclonal anti-β-actin at 1:10,000 (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody dilutions (and suppliers) are as follows: alpha-tubulin DM1A 1:5000 (Thermo Fisher Scientific, Cat# MS581P1); TCF11/NRF1/NFE2L1 D5B10 1:1000 (Cell Signaling Technology ...
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bioRxiv - Genetics 2021Quote: ... and stained with rabbit polyclonal antibodies against collagen XII (KR144;1:200), collagen VI (1:1000, gift from Raimund Wagener, Cologne) and Alexa 488 conjugated wheat germ agglutinin (WGA) (Invitrogen, 1:500), followed by corresponding secondary antibodies coupled to Cy3 (1:800 ...
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bioRxiv - Cancer Biology 2024Quote: ... Collagen was prepared by mixing 500 uL of collagen with 62.5 uL of 10x minimum essential medium (Life Technologies) and neutralized with 0.8 M NaHCO3 to reach a neutral pH ...
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DNA uptake by cell wall-deficient bacteria reveals a putative ancient macromolecule uptake mechanismbioRxiv - Microbiology 2022Quote: ... cells of alpha pKR2 were incubated with a final concentration of 1 mg ml-1 Dextran-Texas Red (3000 MW, neutral, Molecular Probes) in PBS and were imaged overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 488-Phalloidin diluted 1:150 to visualize F-actin and rabbit anti-alpha-actinin-1 (Thermo Fisher Scientific, USA) diluted 1:25 ...
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bioRxiv - Immunology 2022Quote: ... Both recombinant LPS (100ng/ml) and recombinant IFNy (10 ng/ml, Gibco) were provided to cells for differentiation to classical macrophages ...
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bioRxiv - Neuroscience 2021Quote: ... Anthony White (QIMR Berghofer Medical Research Institute, Australia) were maintained on human recombinant vitronectin in StemFlex medium (Life Technologies, cat. # A3349401). Differentiation was performed as previously described [15 ...
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bioRxiv - Systems Biology 2020Quote: ... 72 h after seeding on 96-well microplates differentiated cells were incubated with recombinant human interferon gamma (IFN-γ, ThermoFisher, #PHC4031) at concentrations 0-10 ng/mL or recombinant human interleukin 10 (IL-10 ...
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bioRxiv - Cancer Biology 2020Quote: ... Canada and maintained in keratinocyte serum-free media supplemented with 0.05 mg/ml of bovine pituitary extract and 0.02 μg/ml EGF recombinant human protein (Life Technologies, Benicia, CA) in a 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... coated overnight with 10 μg/ml of xeno-free human recombinant Laminin 521 (LN521, Biolamina) prepared in GMP DPBS+/+ (A1285801, Life Technologies) by reversing the strainer and washing the EBs into the plate with GMP N2 media (CTS™ DMEM-F12 ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated in RP-10 supplemented with 10 ng/ml recombinant human IL-2 and 2 μg/ml anti CD28 (clone 37.51, Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2020Quote: ... HUVECs were treated with 30 ng/ml of VEGF (Recombinant Human Vascular Endothelial Cell Growth Factor, Fisher Scientific 10690920, Bordeaux, France) for 24 hours.
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bioRxiv - Immunology 2021Quote: Purified CD8+ cells were cultured in AIMV medium supplemented with 10% FBS and 100 U/ml recombinant human IL-2 (ThermoFisher Scientific) and used for sorting of CD8+ T cell subsets and for further experiments three days after isolation.
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bioRxiv - Cell Biology 2022Quote: ... 50 mM β- Mercaptoethanol and 1 ng/ml human recombinant leukemia inhibitory factor) for 24 h and then treated with 0.1 μg/ml Colcemid (Gibco, 15210-040) for 2.5 h ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well-plates and treated with 10μg/ml OM-85 concentrate (OM-Pharma) or 100 ng/mL of human recombinant IFN-β protein (ThermoFisher Scientific). The concentration of 10μg/ml of OM-85 was based on previous data with RSV we recently generated [9] ...
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bioRxiv - Biochemistry 2023Quote: ... PIK3CA/PIK3R1 (p110a/p85a) recombinant human protein for use in the experiment shown in Figure 4F was purchased from Thermo Fisher Scientific (catalog number PV4788).