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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Recombinant Human Collagen Type II Alpha 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... His Pur cobalt spin columns (Thermo Scientific) were used to purify the in vitro-translated proteins ...
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bioRxiv - Microbiology 2024Quote: ... 10% HI-FBS (v/v, Gibco 10082147) 100 U/mL penicillin ...
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bioRxiv - Immunology 2024Quote: ... Trichoplusia ni (Hi-5, Thermo Fisher Scientific) and Spodoptera fugiperda (Sf9 ...
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bioRxiv - Developmental Biology 2020Quote: ... The samples were incubated with 1 mg/ml of collagenase Type I (Invitrogen) at 37°C for 15-30 min ...
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bioRxiv - Genomics 2020Quote: ... and bacterial DapB (negative control, Type 1 Probe, VF1-11712, Thermo Fisher Scientific). LECs were additionally co-stained with mouse anti-human CD31 antibody (clone JC70A ...
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bioRxiv - Developmental Biology 2021Quote: ... The testes were then digested with 1 mg/ml type 4 collagenase (Gibco), 1 mg/ml hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... and 80 U mL-1 DNase type I (ThermoFisher Scientific, Waltham, MA, USA) for 25 min in a 37°C shaking incubator ...
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bioRxiv - Molecular Biology 2023Quote: ... hydrogel was incubated in complete media with 1% collagenase type I (ThermoFisher, 17100017) for 25-30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant (Invitrogen, no. 10342-020). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Microbiology 2022Quote: Purified recombinant β-lactamase (ThermoFisher) in PBS (0.15 nM ...
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bioRxiv - Physiology 2021Quote: ... Recombinant CSF1R (Invitrogen, Carlsbad, CA) was used at a concentration of 2.5 nM ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant (Thermo Fisher Scientific, #10342020), and specific oligonucleotides (Supplementary Table S4 ...
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bioRxiv - Cell Biology 2022Quote: ... RPE-1 FRT/TO CCNB1+/+ cell line was transfected with pcDNA5-FRT/TO-Cdk1-alpha-mScarlet and pOG44 (Invitrogen) using a 1:5 ratio ...
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bioRxiv - Bioengineering 2019Quote: ... and the samples stained with 200 µL of 1:100 anti-alpha Tubulin primary antibody (Thermo Fisher Scientific, 322500) for 1 hour ...
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bioRxiv - Neuroscience 2019Quote: Tissue from anonymized donors was received at 4°C in 1 ml “fibroblast medium” containing MEM alpha (Gibco, #12571), 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2021Quote: ... The membranes were incubated overnight with UCP1 antibody and loading control protein alpha tubulin antibody for the total protein adipose tissue samples (1:1.000 UCP1 Polyclonal Antibody, cat no. PA1-24894, 1:500 alpha Tubulin Polyclonal Antibody, cat no. PA5-16891, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 1 week at a density of 106/well in 6 well plates with 50 ng/ml recombinant human macrophage colony stimulating factor (M-CSF) (Life Technologies, Carlsbad, CA), 1x GlutaMAX-I (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: ESCs were maintained on Recombinant Human Protein Vitronectin (Thermo Fischer # A14700) coated plates using mESC maintenance media containing Glasgow Minimum Essential Medium (Thermo Fisher Scientific # 11710035), Embryonic Stem Cell-Qualified Fetal Bovine Serum (Thermo Fisher Scientific # 10439001) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells stably expressing human recombinant LOXL1 were maintained in culture (37°C, 5% CO2) at log-phase growth in advanced DMEM (Thermo Fisher Scientific, 12491015) with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... with 10% FBS (Nucleus Biologics, #AU FBS-500ml L1 HI) and 1% Penicillin-Streptomycin (Gibco, #15140122) and transferred to a new tube ...
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bioRxiv - Neuroscience 2023Quote: ... 2.76 µL master mix 1 (1 µL 5x Superscript II reaction buffer [ThermoFisher] ...
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bioRxiv - Genomics 2023Quote: ... in condΔEXP168 were maintained in 3% hematocrit in human O+ blood in RPMI 1640 supplemented with 5% Albumax-II and 50µg/ml hypoxanthine (Gibco), 25mM HEPES (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then treated with human serum containing phospholipase D (Australian Red Cross Lifeblood) or 0.25% (w/v) Albumax II (Gibco) and incubated for 3 hours at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... were propagated in 5% of human O+ erythrocytes and 10 mL of RPMI-1640 medium containing 0.5% Albumax II (Invitrogen), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2020Quote: ... or 52- or 78-week-old C57BL/6 mice or C57BL/6-TgTM4SF5 mice by perfusion of the liver using collagenase type II (Life Technologies, Carlsbad, CA, USA) (Ateş et al ...
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bioRxiv - Cell Biology 2023Quote: ... and replaced by approximately 1 kb fragments of the C-terminal part of the open reading frame without a stop codon and 3’UTR amplified using wild-type genomic DNA as a template and Phusion Hot Start II high-fidelity DNA polymerase (Thermo Fisher Scientific Baltics, Lithuania) and primers listed in Table S5.
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bioRxiv - Immunology 2023Quote: ... non-essential amino acids (cat no. 11140050) and sodium pyruvate (cat no. 11360070) plus collagenase type II (1mg/mL, cat no. 17101015) (all from Gibco; Grand Island, NY, USA), as described46 ...
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bioRxiv - Cell Biology 2022Quote: ... the human organoids were cultured with 1:1 mix of Human IntestiCult™ OGM Basal Medium and DMEM/F-12 (ThermoFisher) in the presence of 100 IU Penicillin and 100 μg/ml Streptomycin ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... interleukin-6 and -8 (IL-6 and IL-8) human Instant Elisa™ kits and matrix metalloproteinase-1 (MMP-1) human Elisa kit from ThermoFisher Scientific.
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000, 33-3400, Invitrogen; mouse anti-human STAT2 ...
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bioRxiv - Bioengineering 2019Quote: ... and 100ug/mL bovine collagen I (Gibco, A10644-01) dissolved in cold DMEM+1% Pen/Strep ...
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bioRxiv - Developmental Biology 2019Quote: ... HSA+ cells were plated in 0.1% collagen (Life Technologies) for E15.5 or fibronectin/gelatin for postnatal cells ...
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bioRxiv - Cancer Biology 2022Quote: ... and embedded into rat-tail collagen I (Thermo Fisher) as previously described (Nguyen-Ngoc et al. ...
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bioRxiv - Cell Biology 2020Quote: ... that had been pre-coated with rat collagen (Gibco). The cells were pre-stained using a 30 min ...
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bioRxiv - Microbiology 2023Quote: ... with the following extracellular matrix proteins: collagen I (Gibco), collagen IV (Millipore) ...
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bioRxiv - Cell Biology 2023Quote: ... the indicated cells were seeded on collagen I (ThermoFisher, A10483-01 ...
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bioRxiv - Cancer Biology 2024Quote: ... rat tail collagen I (Thermo Fisher Scientific, Waltham, MA) coated ...
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bioRxiv - Cell Biology 2020Quote: To compare the presence of the nucleolar rim of mNG-tagged proteins in live and fixed cells we fixed mNG-tagged cells in 4% formaldehyde (Thermo Scientific, 28908).
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bioRxiv - Cell Biology 2021Quote: ... AnkB440 KO primary cortical neurons were transfected with Halo-tagged AnkB440 or GFP-tagged AnkB220 plasmids at DIV0 by lipofection (Lipofectamine 2000, Thermo Fisher Scientific). After brain dissociation ...
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bioRxiv - Immunology 2022Quote: ... of the different constructs were ordered and cloned into a pPPI4 avidin-tagged and/or hexahistidine-tagged vector by PstI-BamHI digestion and ligation with Gibson Assembly (Thermo Fisher Scientific). The recombinant human ACE-2 receptor was obtained in the same way after ordering the corresponding gBlock gene fragment (Integrated DNA Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... The mCherry tagged SETD6 WT or Y285A and EGFP-tagged E2F1 WT or K117R were co-transfected into cells using polyethyleneimine (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Cell Biology 2019Quote: ... The plasmids encoding for His-SEPT2 or His-SEPT2-mCherry and SEPT6/7-strep were co-transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 2-3 and induced with 1 mM IPTG for 1h at 37°C (His-SEPT2 ...
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bioRxiv - Immunology 2021Quote: ... RBD-His and Spike-his containing supernatants were batch purified using the HisPur™ Ni-NTA Resin (ThermoFisher Scientific). Supernatants were incubated with 6 ml of resin for 1h at RT ...
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bioRxiv - Bioengineering 2024Quote: ... and human and mouse DKK3 (hDKK3: AA 1-350, mDKK3: AA 1-349) triple tagged with Fc-Myc-6xHis were transfected into Expi293FTM (Thermo Fisher Scientific) cells at a density of 3 × 106 viable cells/mL using ExpiFectamineTM (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... mice were inoculated with 500,000 DIPG17-luciferase tagged cells in 1:1 suspension (200 μl total) with full media and MatrigelTM (Fisher Scientific CB-40234) via subcutaneous injection in the left flanks ...
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bioRxiv - Cell Biology 2023Quote: ... Human Jurkat T cells and human THP-1 monocytes were cultured in RPMI 1640 medium (ThermoFisher), supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... 100 pmol (77 nM) of recombinant dynein tail complex or recombinant protein A (ThermoFisher Scientific) were incubated with 100 μL IgG magnetic beads in 1300 μL IgG binding buffer with rotation for 1 h at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... The secondary antibodies tagged with Alexa Fluor 647 (Invitrogen) at 5 μg/mL were added to the slices and incubated for 1 h before three-times washing and imaging.