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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... HEK293 cells were transfected in 6 well plates at 60% confluence using lipofectamine LTX (Invitrogen) with 1µg of each transposon vector along with 0.5µg of pCMV-m7PB hyperactive transposase (34) ...
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bioRxiv - Bioengineering 2024Quote: ... All other CYpHER molecules were produced by transient expression in suspension HEK293 cells (ThermoFisher GeneArt) and purified either via Ni-NTA pull-down as previously described (33 ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293/CRE-Luc/hCXCR4 cells were plated in Opti-MEM media (Invitrogen, cat# 31985-070) supplemented with 0.1% FBS (Seradigm ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were co-transfected with 200 ng of GeneArt CRISPR Nuclease mRNA (Invitrogen #A29378) in addition to sgRNA prepared by mixing fluorescently labelled Alt-R CRISPR-Cas9 tracrRNA ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293 cells were incubated for 1.5 hours with glutamate-free DMEM GlutaMAX-I (Life Technologies), to reduce the extracellular concentration of glutamate ...
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bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney fibroblasts (HEK293, RRID:CVCL_0045) were grown in Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher) supplemented with 10% fetal calf serum (FBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Transient transfection of HEK293 cells by plasmid DNA was carried out by Lipofectamine 2000 (Invitrogen). The cells were replated 2 days after transfection and treated with 1 mg/mL G418 sulfate (EMD Chemicals Inc. ...
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bioRxiv - Microbiology 2023Quote: ... MRC-5 and HEK293-FT cells were grown in DMEM with GlutaMAX (Thermo Fisher # 10566016). CHO-K1 ...
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bioRxiv - Biophysics 2023Quote: ... The construct was transfected in HEK293 cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA #11668019), following the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: HEK293 cells were grown and maintained in high-glucose (4.5 g/l) DMEM (Thermo Fisher) for BRET assays ...
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bioRxiv - Genetics 2023Quote: HEK293 cell protein was extracted with 0.3 mL of RIPA buffer (ThermoFisher, Cat. No. 89900) containing Protease Inhibitor Cocktail (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... HEK293 cells were transfected with appropriate plasmids using either Lipofectamine 3000 (Thermo Fisher Scientific Inc.) or JetPrime (Polyplus Transfection Inc. ...
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bioRxiv - Developmental Biology 2023Quote: HEK293 and P19 cell lines were grown in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, C11995500BT) supplementary with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: HEK293S GnTI- cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher Scientific, cat# 21969035) supplemented with 10 % (v/v ...
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bioRxiv - Immunology 2022Quote: ... 1 mln HEK293 Flp-In T-REx cells were grown on DMEM High glucose (Gibco) supplemented with 10% fetal bovine serum on 6-well plates ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were plated at 50% confluency overnight and transfected using Lipofectamine 3000 kit (ThermoFisher) with ∼0.5 μg of CPEB3 DNA ...
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bioRxiv - Biophysics 2023Quote: ... Stable HEK293 cell lines were maintained in phenol red-free DMEM media (Gibco, Waltham, MA) supplemented with 2 mM GlutaMAX (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: ... The vectors were then transfected into human embryonic kidney cells (HEK293) with Fectin-system (Invitrogen). Protein from culture supernatant was isolated as previously described [46 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 and RAW 264.7 cells were obtained from ATCC and were cultured in DMEM (Gibco) supplemented with 10% FBS (Wisent ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells (CRL-1573, ATCC, female) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The HEK293/Flp-In/T-Rex were additionally cultured with 1x penicillin/streptomycin (ThermoFisher 10378016). To begin experiments ...
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bioRxiv - Biochemistry 2023Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s serum (DMEM) (Thermo Fisher Scientific, Oberhausen, Germany) supplemented with 10% FBS (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... pH change experiment for oROS-HT-C199S were performed with HEK293s in PBS (10010001, Gibco) prepared at pH of 6 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HEK293 cells were transiently transfected following the reverse transfection method using LipofectamineTM 3000 (Thermo Fisher). Two transfection mixes (A & B ...
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bioRxiv - Genomics 2024Quote: Human HEK293 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Cat# 084564, Gibco, US) supplemented with fetal bovine serum (10% ...
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bioRxiv - Biochemistry 2024Quote: SH-SY5Y and HEK293/T cells were transfected with siRNA using Lipofectamine 3000 (Thermo Fisher) per the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Transfections were performed in HEK293 cells using Lipofectamine™ 3000 Transfection Reagent (L3000015, Thermo Fisher) and serum-free Opti-MEM I medium (31985 ...
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bioRxiv - Neuroscience 2024Quote: ... sgRNAs were tested for efficacy in HEK293 (ATCC) using a genomic cleavage kit (GeneArt, ThermoFisher). The best sgRNA was used for editing and had the sequence ...
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bioRxiv - Cell Biology 2020Quote: ... Released IL-1β was quantified using the Human IL-1 beta ELISA Ready-Set-Go! Kit (ThermoFisher) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... previously cultured for 24h with IL-15/IL-15R complex recombinant protein (100ng/ml, Thermo Fisher Scientific) and retinoic acid (100ng/ml) ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... The concentration of IL-6 was quantified by ELISA (Human IL-6 ELISA Ready-SET-Go!, Thermofisher) by following the supplier’s protocol.
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bioRxiv - Microbiology 2023Quote: ... the supernatants were collected and we measured the IL-1β concentrations using IL-1β ELISA kits (Invitrogen) and following manufacturer instructions.
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bioRxiv - Microbiology 2021Quote: ... overnight at 4 °C followed by Goat-anti Mouse IgG-HRP secondary antibody (Invitrogen™) at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2019Quote: Freshly dissected mouse brains were quickly frozen in a 2-methylbutane (Fisher Scientific #O3551-4) dry ice bath ...
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bioRxiv - Microbiology 2021Quote: ... 20 min incubation at 4°C with anti-mouse IgG2a-AF546 or -AF488 (both ThermoFisher) was applied ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse tibias and spleens were fixed in 10% neutral buffered formalin (Fisher Scientific #SF100-4) overnight at 4°C or 1 hr at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... AlexaFluor-488-conjugated goat anti-mouse IgG (4 µg/ml, Invitrogen, catalog # A-11001, RRID:AB_2534069), AlexaFluor-488-conjugated donkey anti-rabbit IgG (4 µg/ml ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Proteins (20 μg per mouse sample) were loaded on 4–12% Bis-Tris gels (ThermoFisher), separated by SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: H&E staining for mouse tissues were fixed in 10% formalin (SF98-4, Thermo Fisher) processed in UF pathology core ...
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bioRxiv - Neuroscience 2023Quote: Freshly dissected mouse brains were quickly frozen in a 2-methylbutane (Fisher Scientific #O3551-4) dry ice bath ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4 °C followed by Goat-anti Mouse IgG-HRP secondary antibody (Invitrogen™) at room temperature for 2 hours ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were differentiated with GM-CSF (800U/ml) and IL-4 (500U/ml) in enriched RPMI (Gibco, 10% bovine serum, P/S, 2mM Glutamine) in a 5% CO2 humidified 37°C incubator as previously described (Geijtenbeek et al. ...
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bioRxiv - Physiology 2019Quote: ... The total protein of the kidneys was extracted by centrifugation at 14,000g for 15 minutes at 4°C and protein concentration was determined using micro bicinchoninic acid (BCA) protein assay kit (Thermo Scientific, Rockford, Il, USA) according to manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2021Quote: ... and centrifuged at 13000×g for 20 min at 4°C.The protein concentrate on of collected supernatant was determined using bicinchoninic acid (BCA) assay (23225; Thermo Fisher Scientific, Rockford, IL, USA). An equal amount of each protein sample was electrophoresed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: 1 × 106 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 or PBS for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before being flash frozen in liquid nitrogen ...
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bioRxiv - Immunology 2023Quote: 5×105 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before total RNA was isolated from cells by Purelink™ RNA Mini Kit (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were stimulated with IL-4 (20ng/mL) for 24 hours before lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). 75,000 DiO (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were stimulated with IL-4 (20ng/mL) for 24 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were diluted to a concentration of 1 million cells/mL and 50uL of cell solution was plated on a precoated 4ug/mL fibrinogen and 0.5% polyvinyl alcohol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were treated with 20ng/mL IL-4 for 5 hours before cells were detached using PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were then stained with a combination of Mitotracker Green FM ...
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bioRxiv - Immunology 2019Quote: ... Samples were prepared using the Ion PGM™ Hi-Q™ View OT2 and Ion PGM™ Hi-Q™ View Sequencing kits (ThermoFisher Scientific) and 4 barcoded libraries were combined per Ion 316™ Chip kit (ThermoFisher Scientific) ...