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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... The inter-molecular tau FRET biosensor was generated by transiently transfecting HEK293 cells using Lipofectamine 3000 (Invitrogen) with tau-GFP and tau-RFP (1:20 DNA plasmid concentration ratio) ...
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bioRxiv - Molecular Biology 2021Quote: ... were transfected into HEK293 T-Rex cells with Lipofectamine CRISPRMAX Cas9 Transfection Reagent (Thermo Fisher Scientific, CMAX00008). After 48 h of incubation ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 stable cells overexpressing Flag-INTS118 and Flag-Ago215 were cultured in DMEM media (Gibco, #11965-084) containing puromycin and supplemented with 10% FBS (Atlas Biologicals ...
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bioRxiv - Cancer Biology 2019Quote: ... Both HEK293 and HEK293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco/Invitrogen, Inc., USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Both HEK293 and HEK293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco/Invitrogen, Inc., USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: Human Embryonic Kidney 293 cells (HEK293, ATCC CRL-1573) were grown under standard conditions in DMEM (Gibco) supplemented with 10% FBS (BioConcept) ...
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bioRxiv - Molecular Biology 2020Quote: Plasmids encoding RiboTag constructs were used to transiently transfect HEK293 cells with lipofectamine 3000 (Thermo Fisher Scientific) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (FBS; Invitrogen) and 200 U/ml of penicillin and streptomycin (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: HEK293 cells were grown and maintained in DMEM/F12 medium (Gibco/Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FCS (Eurobio ...
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bioRxiv - Cell Biology 2021Quote: Cells including MEFs and STIM1-KO HEK293 cells (Emrich et al., 2019) were maintained in DMEM (Invitrogen) supplemented with 10% FBS and 1% Penicillin/Streptomycin (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: HEK293 FT cells were transfected with dVPR and VSVG packaging plasmids using Lipofectamine 2000 reagent (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... TAP cell lines were generated based on the HEK293 Flp-In T-REx system (Thermo Fisher Scientific). HEK293 cell lines with tetracycline inducible TAP-tagged baits were generated by co-transfection of 0.1 µg of plasmid containing the TAP-tagged bait (hsINTS13ΔC ...
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bioRxiv - Neuroscience 2023Quote: The human embryonic kidney cells (HEK293 and HEK293T) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 15% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were seeded in 10 cm2 dish with Dulbecco’s Modified Eagle Medium (#11965118, Thermo Fisher Scientific) supplemented with 25 mM HEPES ...
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bioRxiv - Bioengineering 2023Quote: ... HEK293 6E cells were used for transient production of these proteins following standard protocols for 293Fectin (Invitrogen). The cell cultures were harvested at 5 d post-transfection by centrifugation at 6,000 rpm for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... HeLa-FRT UBAP2L KO and HEK293 cell lines were cultured in DMEM GlutaMax media (Thermo Fisher Scientific) at 37C in a humidified incubator with 5%CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP-APEX2-NIK3 expressing U2OS (U2OS:GFP-APEX2-NIK3) and HEK293 cells were cultured in Dulbecco’s modified eagle’s medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2023Quote: Flp-In™ T-REx™ 293 cells (referred to as HEK293 through the manuscript; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5ug of each plasmid was transfected independently into HEK293 cells using lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). Forty-eight hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... and HEK293 (NT and FASTKD5 sgRNAs) samples were purified using the Dynabeads mRNA purification kit (ThermoFisher, 61006) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... We cultured the cells in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen; RAW 264.7, HEK293, and NIH-3T3) or F-12K medium (Caisson ...
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bioRxiv - Developmental Biology 2023Quote: HEK293 cells were transfected with 3 μg of linearised plasmid using Lipofectamine 3000 Transfection Reagent (Invitrogen, #L3000001) in wells of a 6-well plate ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 LgBit cells (gift from Itzhaki lab) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, ThermoFisher, 41966052) supplemented with L-glutamine (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: Human neuroblastoma SH-SY5Y cells (ATCC) or HEK293 (ATCC) cells were cultured in DMEM/F12 media (Gibco) or DMEM High-glucose (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... ISF Aβ42 in the 14 samples for each mouse was measured by ELISA (Invitrogen, KHB3441, 1:4). To determine Aβ42 half-life (Cirrito et al. ...
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bioRxiv - Neuroscience 2020Quote: ... was incubated overnight at 4 °C and anti-mouse-HRP-coupled secondary antibody (1:3000, Life Technologies) was incubated for 1.5 h at RT ...
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bioRxiv - Plant Biology 2020Quote: ... 4 hour incubation in MTSB containing 2 % BSA and 0.5 % Alexa-conjugated anti-mouse secondary antibody (Invitrogen), 9 washes for 15 minutes in MTSB ...
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bioRxiv - Genetics 2022Quote: ... at room temperature then incubated overnight at 4°C with mouse anti-v5 (1:1000, Invitrogen, RRID:AB_2556564), and rat anti-tubulin alpha (1:10,000 ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with primary antibodies overnight at 4 °C (1:100 mouse anti CYP3A4 antibody, Life Technologies MA5-17064 ...
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bioRxiv - Microbiology 2019Quote: ... Splenocytes were then stained for CD3 (4 μg/ml eFluor 450 a-mouse CD3 antibody; ThermoFisher Scientific) and CD8 (2 μg/ml APC/Cy7 a-mouse CD8a antibody ...
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bioRxiv - Physiology 2019Quote: ... Beads used for cell stimulation were generated by incubating 4×106 CELLection Pan Mouse IgG beads (ThermoFisher) with antibodies (BD Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were incubated overnight at 4 ºC in primary mouse anti-human αSMA monoclonal antibody (Fisher Scientific catalog no.
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bioRxiv - Cell Biology 2023Quote: ... at 4 °C overnight respectively and anti- mouse secondary antibody conjugated to Alexa Fluor 488 (Life Technologies) at room temperature for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: IL-1β levels were determined from prefrontal cortex extracts by using the IL-1β ELISA kit (ThermoFisher Scientific, #88-7013) following the manufacturer’s protocols.
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bioRxiv - Immunology 2021Quote: IL-17a ELISAs were performed using Human IL-17A (homodimer) ELISA Ready-SET-Go!™ Kit (Invitrogen™; eBioscience™), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: IL-8 and IL-1β cytokines were quantified by ELISA using the Rabbit IL-1 beta ELISA Kit and Rabbit IL-8 ELISA Kit (Invitrogen). Aliquots of 2 mL of lung homogenate shred in saline solution were centrifuged during 30 min at 10 000 rpm and 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein was produced from IL-2 and IL-12 expressing stable lines during one-week culture in serum-free media (Freestyle 293, Invitrogen) and purified with HisPur Ni-NTA affinity resin (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... and stimulated for 24 hours with IL-17A (200ng/mL, cat: 200-59-100ug, Shenandoah) and anti-IL-17RC (5ug/mL, cat: PA5-47286, Invitrogen). Goat IgG (5ug/mL ...
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bioRxiv - Microbiology 2023Quote: Interleukin-6 (IL-6)_ and interleukin-12 (IL-12_ expression levels were detected by ELISA Kits (Thermo Fisher Scientific, USA); the samples analysed via ELISA were either plasma drawn from mice or cell culture supernatants ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 12.5 ng/mL IL-7 (Miltenyi, USA) and 12.5 ng/mL IL-15 (Miltenyi, USA) or RPMI 1640 (Gibco, USA) medium supplemented with 100 U/mL IL-2 (Sci-Store ...
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bioRxiv - Bioengineering 2024Quote: WT IL-10 and Fab-IL-10 constructs were fluorescently labeled using Alexa Fluor 647 N-hydroxysuccinimide (NHS) ester (Invitrogen), and unreacted dye was removed with a Zeba Spin Desalting Column (7K MWCO ...
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bioRxiv - Immunology 2024Quote: ... IL-6 levels in the supernatant and plasma were determined using a high sensitivity IL-6 ELISA Kit (BMS603HS, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at room temperature for 1 h with secondary antibodies Alexa Fluor 568 goat anti-mouse IgG and Alexa Fluor 488 goat anti-rabbit IgG (1:500; Invitrogen Life Technologies, Rockford, IL, USA), counterstained with Hoechst 33258 (1:1,500 ...
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bioRxiv - Microbiology 2023Quote: ... at 4°C and incubated with mouse anti-HA mAb clone 12CA5 conjugated and crosslinked to protein A/G magnetic agarose beads (Pierce; Rockford, IL. Thermo Fisher Scientific. Waltham, MA, USA). After rotation at 4°C for 1 h ...
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Extensive introgression among North American wild grapes (Vitis) fuels biotic and abiotic adaptationbioRxiv - Evolutionary Biology 2021Quote: ... Nanodrop 2000 spectrophotometer (Thermo Scientific, Hanover Park, IL) was then used to evaluate RNA purity ...
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bioRxiv - Immunology 2022Quote: ... and recombinant murine IL-18 (Thermo Fisher Scientific) at 1 ng/ml unless otherwise specified ...
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bioRxiv - Immunology 2021Quote: ... IL-17 (eBio17B7, Thermo Fisher Scientific, Waltham, MA), and IFNγ (XMG1.2 ...
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bioRxiv - Neuroscience 2019Quote: ... and protease inhibitor cocktail (Thermo Scientific, Rockford, IL)] ...