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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Human integrin alpha 5 beta 3 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... minced skin was incubated at 37°C for 3 – 5 hours in 5 ml of DMEM high glucose (#41965-039; Gibco) supplemented with 10 mg ml-1 collagenase (#C9891 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 1.5 µg of total RNA of each sex were subjected to 5’ and 3’ RACE with a GeneRacer kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... BM was flushed from cut femurs and tibiae using 3-ml syringes with Minimum Essential Medium Alpha (αMEM without ribonucleosides or deoxyribonucleosides; Gibco, Life Technologies cat. # 12561-056, Grand Island, NY) supplemented with 15% fetal calf serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and T2/Onc2.r1 5’-ATC TAT GGC TCG TAC TCT ATA GGC-3’ designed to amplify the pT2/Onc2 vector excluding the MSCV 5’ LTR minimal promoter and Lun-SD sequences using platinum Hi-Fi DNA Taq polymerase (Invitrogen #11304-028) in 1X Hi-Fi PCR buffer (Invitrogen #52045 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
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bioRxiv - Cell Biology 2020Quote: ... anti- HA-tag for KRAB protein (1:500, 32-6700 NOVEX HA epitope Tag antibody 5B1D10, Invitrogen, USA) and anti-b-Catenin (1:400 ...
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bioRxiv - Cell Biology 2024Quote: ... Myc-tag immunoprecipitation (IP) was carried out using the Myc-tag magnetic IP kit (Thermo Fisher Scientific, 88844), following the manufacturer’s recommended protocol.
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bioRxiv - Immunology 2020Quote: ... The C-tagged SARS-CoV2 S trimers were purified using a two-step purification protocol by 5 mL CaptureSelect™ C-tag Affinity Matrix (ThermoFisher Scientific). Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
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bioRxiv - Genomics 2023Quote: Levels of H3K27ac CUT&Tag binding signal was determined by qPCR amplification carried out with the QuantStudio™ 5 Real-Time PCR System (ThermoFisher A34322) using the Standard Curve experiment type and SYBR Green Master Mix (ThermoFisher 4309155) ...
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bioRxiv - Cell Biology 2020Quote: ... anti-S-tag (Thermo Scientific, #MA1-981), anti-TMEM43 (Abcam ...
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bioRxiv - Biochemistry 2020Quote: ... mouse anti-V5-Tag (46-0705, Invitrogen), mouse anti-Myc-Tag (9B11 ...
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bioRxiv - Neuroscience 2020Quote: ... tag from pcDNA3.1-nV5-DEST vector (Invitrogen). To generate AP2M1 or LRRK2 single or multiple mutants ...
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bioRxiv - Microbiology 2022Quote: ... anti-FLAG tag (Thermo Fisher (PA1-984B)).
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bioRxiv - Microbiology 2021Quote: ... or DYKDDDDK Tag Monoclonal Antibody (FG4R) (Invitrogen) (1 ...
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bioRxiv - Biochemistry 2021Quote: ... and V5-tag (R96025) from Life Technologies.
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bioRxiv - Cell Biology 2019Quote: ... mouse Flag-Tag monclonal antibody(Thermo Fisher, WB 1:1000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... anti-KDDDDK Tag antibody (Invitrogen, MA1-91878), Monoclonal antibody anti-GAPDH produced in mouse (SIGMA ...
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bioRxiv - Immunology 2021Quote: ... AccuPrime Tag DNA polymerase High Fidelity (Invitrogen) with initial denaturation at 94 °C for 2 min ...
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bioRxiv - Cell Biology 2022Quote: ... V5-tag antibody (Cat # R960-25, Invitrogen), GST antibody (Cat# 2622S ...
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bioRxiv - Microbiology 2023Quote: ... or anti S-tag (MA1981, Life Technologies). Proteins were detected using horseradish peroxidase (HRP)-conjugated goat anti-rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... HA tag (Invitrogen, MA5-27915, 1:1000), or cyclophilin B (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... V5 tag (R960-25, from Thermo Fisher), Nrf1 (this specific antibody made in our own laboratory [40]) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-V5 tag (Invitrogen, catalog no.R960-25).
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bioRxiv - Microbiology 2024Quote: ... and anti-Xpress tag antibody R91025 (Invitrogen). Anti-PGK1 antibody 22C5D8 (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-Strep-tag II (NC9261069, Thermo Fisher), anti-FLAG epitope tag (L00018 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies include: anti-V5 Tag (Invitrogen, ref R96025 ...
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bioRxiv - Genetics 2024Quote: ... The FISH Tag DNA Multicolor Kit (Invitrogen) was used for nick translation and chemical labelling with Alexafluor (AF ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated for 3 h at 4 °C with 50 mL of Pierce Protein-G beads bound with mouse anti-Ty antibody (Invitrogen Ty1 Tag Monoclonal BB2). Beads were washed 6 times with wash buffer (200 mM Tris pH 9 ...
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bioRxiv - Cell Biology 2019Quote: ... The plasmids encoding for His-SEPT2 or His-SEPT2-mCherry and SEPT6/7-strep were co-transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 2-3 and induced with 1 mM IPTG for 1h at 37°C (His-SEPT2 ...
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bioRxiv - Immunology 2021Quote: ... RBD-His and Spike-his containing supernatants were batch purified using the HisPur™ Ni-NTA Resin (ThermoFisher Scientific). Supernatants were incubated with 6 ml of resin for 1h at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... n = 3) or oligopyridylamides (5 µM ADH-1 or ADH-6, n = 3) using a combination of both TriZol (Thermo Fisher Scientific) and RNAeasy Mini Kit (Qiagen ...
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bioRxiv - Plant Biology 2022Quote: 5’-biotinylated RNA and unmodified RNA of Motif 3 (5’-AAAUCGCCGGAG-3’ 5’-Bio-AAAUCGCCGGAG-3’) were synthesized by Eurofins (Hamburg, Germany) and used for LightShift™ Chemiluminescent RNA EMSA Kit (Thermo Scientific). Total protein was extracted from LL and 10 min LL➔HL-treated plants using extraction buffer (25 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... the hypervariable V3 region of the 16S rDNA gene was amplified from 20 ng of DNA using the primers 5′-CCTACGGGAGGCAGCAG-3′ and 5′-ATTACCGCGGCTGCTGG-3′ (Integrated DNA Technologies BVBA, Leuven, Belgium),(18) 1U Platinum® PCR SuperMix High Fidelity (ThermoFisher Scientific) and 10 μM of primer-mix ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in 0.05 M Tris-HCl buffer (pH 7.6) containing 5 mg 3-3′ diaminobenzidine (Thermo Scientific, TA-060-HDX) per 10 mL buffer and a final concentration of 0.01% hydrogen peroxide for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: ... and hybridized first using RT primer (5′/5Biosg/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3′) and then reverse transcribed into cDNA using TSO primer (5′-AAGCAGTGGTATCAACGCAGAGTACATrGrGrG-3’) and RT maxima reverse transcription (Thermo Fisher Scientific). cDNA was amplified using ISPCR primer (5′-AAGCAGTGGTATCAACGCAGAGT-3′ ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Cell Biology 2023Quote: ... Treatments were incubated 2 hours before addition of the MTS (3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) reagent (Thermo-Fisher Scientific, Waltham, MA) and incubation was continued another 1.5 hours ...
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bioRxiv - Microbiology 2024Quote: ... and a pan VSG reverse primer which binds to a conserved 14bp region of the 3’ UTR (5’-GATTTAGGTGACACTATAGTGTTAAAATATATC-3’) with AmpliTaq Gold (Applied Biosystems, 4398881) (anneal & extension 60C 1m ...
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bioRxiv - Microbiology 2020Quote: Parasites after treatment were washed in 5 mL complete medium at 400 g for 3 min and stained with 5 μM JC-1 (ThermoFisher Scientific) in complete medium in the dark at 37 °C for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: The detection of TGF-β in the cell culture media from SH-SY5Y and MDA-MB-231 cells treated with TEPA for 24h was performed according to the protocol suggested by Thermo Fisher (TGF-beta-1-Human-ELISA-Kit/BMS249-4).
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bioRxiv - Cell Biology 2020Quote: ... Human iPSC lines were maintained in 5% CO2 environment in Essential 8 Medium (Thermo Fisher). For cardiomyocyte differentiation ...
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bioRxiv - Developmental Biology 2021Quote: ... Mouse and human sperm were loaded with Cal-520-AM (5 μM, Molecular Probes, USA) in the presence of Pluronic F-127 (0.02% v/v ...
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bioRxiv - Cell Biology 2022Quote: Human HEK293 cells were cultured at 37 °C and 5% CO2 in DMEM (ThermoFisher Scientific), supplemented with 10% fetal bovine serum (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... for 5 min followed by staining with an anti-human Alexa FluorTM 555 antibody (ThermoFisher). Coverslips were mounted on glass slides using ProLongTM Gold Antifade Mountant containing DAPI (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... astrocytes and human iMGLs on PDL-coated round 5 mm glass coverslips (Thermo Scientific Menzel) at density of 7000-10000 cells/coverslip ...
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bioRxiv - Cell Biology 2022Quote: ... human HEK 293T cells were cultured at 37 °C in 5% CO2 in DMEM (ThermoFisher), with 10% FCS and 1% P/S ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant human Epithelial Growth Factor 5 µg.L-1 (Ref AF-100-15; PEPROTECH, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... for human β-globulin the mastermix was made using 5 μL Fast SYBR Green (ThermoFisher), 1 μL of hbg1 primer (3 μM) ...
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bioRxiv - Bioengineering 2021Quote: ... Human lung (Calu-3) (ATCC, HTB-55) cells were cultured in Minimum Essential Media (MEM) (Gibco; 11095080) supplemented with 10% FBS with 1mM sodium pyruvate (Gibco ...
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bioRxiv - Bioengineering 2021Quote: T cells from 3 donors were thawed and activated using Dynabeads Human T-Activator CD3/CD28 (ThermoFisher) for 24 hours ...