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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Allogenic CD8+ T-cells were isolated by negative selection using Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen), labeled with carboxyfluorescein succinimidyl ester (CFSE ...
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bioRxiv - Immunology 2019Quote: ... Dynabeads Human T-Activator CD3/CD28 for T-cell expansion and Activation kit (1132D) were purchased from Thermofisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... total RNA were extracted then reverse transcribed using the Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermofisher Scientific). The cDNA libraries were amplified and barcoded using Ion AmpliSeq Transcriptome Human Gene Expression core panel and Ion Xpress Barcode Adapter (Thermofisher Scientific) ...
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bioRxiv - Synthetic Biology 2021Quote: BMP4 concentration released into the media was quantified using the BMP-4 Human ELISA Kit from (ThermoFisher, EHBMP4). Standards and protocols were done according to the instructions of the kit ...
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bioRxiv - Immunology 2021Quote: ... and anti-Spike IgG was quantified using a Human SARS-CoV-2 Spike (Trimer) IgG ELISA Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining was performed using buffer sets of Human Regulatory T-cell Staining Kit (eBioscience/Thermo Fisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Isolation of CD3+ T-cells was performed using Dynabeads® Untouched™ Human T-cells Kit (11344D, Invitrogen). CD3+ T-cells were stained with CellTrace Violet Cell Proliferation Kit (C34571 ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were isolated from PBMCs using the Dynabeads® Untouched Human T Cells Kit (Invitrogen, Karlsruhe, Germany) following the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... labeled with Alexa Fluor 488 from the Zenon™ Human IgG Labeling kit (Molecular Probes, Eugene, OR, USA) and 15 μg/mL of 7-amino-actinomycin D (7-AAD ...
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bioRxiv - Immunology 2022Quote: ... and CD4+ and CD8+ T cells were further isolated with Dynabeads Human CD4+ or CD8+ Isolation Kits (ThermoFisher), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Total tau content was quantified in brain extracts by ELISA (Human Tau (total) ELISA kit (#KHB0041, Invitrogen, UK), see supplementary methods ...
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bioRxiv - Microbiology 2023Quote: ... IL-1β secretion from PMA-THP1 was detected using IL-1 beta Human ELISA kit (#KHC0011, ThermoFisher Scientific) and IL-1 beta Human ELISA kit ...
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bioRxiv - Bioengineering 2023Quote: ... The plasma insulin concentration was determined using a Human Insulin ELISA kit according to the manufacturer’s protocol (Invitrogen). The relative bioavailability (RA ...
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bioRxiv - Immunology 2023Quote: ... IL-18 and human IL-1β was analyzed in the cell culture supernatant by commercial ELISA kits (Invitrogen) following the manufacturer’s recommended procedures.
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bioRxiv - Neuroscience 2024Quote: Human fibroblast cells were reprogrammed using the Sendai virus-based CytoTune iPS 2.0 kit (CytoTune 2.0, Invitrogen, A16517). Fibroblasts were plated in six-well plates two days before transduction to achieve a density of 2 × 105 to 3 × 105 cells per well ...
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bioRxiv - Immunology 2020Quote: ... by non-integrative Sendai Viral vector kit (CytoTune™-iPS Sendai Reprogramming Kit encoding for Oct4, Sox2, KLF4 and c-Myc, Life Technologies Cat.No.A1378001) by the Harvard Stem Cell Institute (HSCI ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of RNA were reverse transcribed and amplified using TaqMan™ RNA-to-C T ™ 1-Step Kit kit on the ViiA 7 Real-Time PCR System (Applied Biosystems). TaqMan gene expression assay probes (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: Gene fragments (gBlocks) were cloned into pCR-Blunt II-TOPO vector using the Zero Blunt TOPO PCR Cloning kit (Invitrogen) and their sequences were verified by Sanger sequencing ...
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bioRxiv - Biochemistry 2020Quote: The DHTKD1 variants were constructed using the QuikChange® II site-directed mutagenesis kit (Thermo Fisher Scientific Inc., MA, USA), the pET28a(+ ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA concentration was determined using Nanodrop and 500-1000ng of RNA was used to synthesize complementary DNA using Superscript II kit (Invitrogen). For RT-qPCR the MESA Blue qPCR MasterMix Plus kit was used (Eurogentec) ...
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bioRxiv - Neuroscience 2021Quote: Loss of function experiments were performed with miRNAs generated using the BLOCK-iT Pol II miRNA Expression Vector Kit (Invitrogen) (Table S1) ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA from different samples were used to synthesize cDNA using Superscript II reverse transcription kit (Invitrogen, Inc., Carlsbad, CA). 1μl of 10X dilution of cDNA was used per reaction for real-time quantitative PCR in a total volume of 10 μl using SYBR green reagent ...
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bioRxiv - Plant Biology 2021Quote: ... The products were cloned using a pENTR/D-TOPO Cloning Kit and transferred into pK7GWIWG2(II)-RedRoot using Gateway LR reactions (Invitrogen). The 35S:RAM1 cassette from pK7WG2R was amplified using the primer pair listed in Table S1 and cloned into pK7GWIWG2(II)-RedRoot at the Kpn1 and Xho1 sites using T4 DNA ligase (NEB) ...
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bioRxiv - Plant Biology 2021Quote: ... PCR with RNA template was used to confirm the absence of gDNA before cDNA synthesis using the Superscript II retrotranscription Kit (Invitrogen). PCR to confirm expression of the transgene was conducted using gene specific primers ...
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bioRxiv - Neuroscience 2021Quote: ... This fragment was subcloned into pCR Blunt II TOPO vector using the Zero Blunt TOPO kit (ThermoFisher Scientific cat# K287540). In parallel ...
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bioRxiv - Cancer Biology 2020Quote: ... Target preparation was performed using an Eberwine-based amplification method with Amino Allyl MessageAmp II aRNA Amplification Kit (Ambion, AM1753) to generate amino-allyl antisense RNA (aa-aRNA) ...
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bioRxiv - Cell Biology 2020Quote: ... The fragments were cloned into pCR-Blunt II TOPO using the Zero Blunt TOPO PCR Cloning Kit (Thermo Fisher Scientific), and the sequencing was verified using M13 forward and M13 reverse universal primers ...
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bioRxiv - Microbiology 2022Quote: ... the HCV genome was amplified in three overlapping fragments via nested PCR using the platinum SuperFi II DNA polymerase kit (Invitrogen) with oligonucleotides as specified in Table S1 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of age-specific total RNA was used to synthesize cDNA libraries with oligoDT (Superscript II First-Strand Synthesis kit, Invitrogen). 1μg of cDNA was then used with gene-specific primers (see supplemental table S2 ...
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Gene expression in isolated cowpea (Vigna unguiculata L. Walp) cells from meiosis to seed initiationbioRxiv - Plant Biology 2020Quote: ... RNA was subjected to two rounds of amplification (three rounds for fTET, ES2n and ES4n) using the MessageAmp II RNA amplification kit (Ambion). Total RNA was extracted from leaf as described (Spriggs et al. ...
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bioRxiv - Bioengineering 2019Quote: ... RNA extraction was carried out using the Macherey-Nagel Nucleospin RNA kit and cDNA synthesis was done with Superscript II Reverse Transcriptase (Invitrogen) from 1 μg of RNA ...
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bioRxiv - Molecular Biology 2019Quote: ... We reverse transcribed total RNA with random hexamer primers using the Superscript II Reverse Transcriptase kit (Thermo Fisher, 18064-022). We performed qPCR with SYBR Green PCR Master Mix (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... were designed against the mouse Auts2 coding sequence using the BLOCK-iT Pol II miR RNAi Expression Vector Kit guidelines (Invitrogen). Auts2 miRNA constructs were subcloned into the pCL20c-trL7 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Amplicons exceeding the estimated minimum were gel purified as described above and cloned into the pCR Blunt II-TOPO vector using the Zero Blunt TOPO PCR Cloning Kit (Invitrogen). Ligated plasmids were transformed into One Shot TOP10 Chemically Competent E ...
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bioRxiv - Neuroscience 2021Quote: ... the PCR products from using the primer OT-F and OT-R1 were subcloned into the pCR Blunt II TOPO vector (Zero Blunt TOPO PCR Cloning Kit, ThermoFisher) and sequenced using M13-Foward and M13-Reverse primers ...
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bioRxiv - Cell Biology 2020Quote: ... eGFP mRNA was transcribed in vitro from a linearized pBluescript II SK+ construct using the T7 mMessage Ultra kit (Ambion/ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Amplification products were purified using an Isolate II PCR and gel kit according to manufacturer’s instructions (Invitrogen, Carslbad, California, USA). Sanger sequencing of both strands of each PCR product was carried out commercially ...
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bioRxiv - Immunology 2021Quote: ... reverse transcription (RT) of 200 ng of RNA was carried out using the Super Script II reverse transcriptase kit (ThermoFisher). Real time quantitative PCR (RT-qPCR ...
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bioRxiv - Immunology 2020Quote: ... naïve OT-I and OT-II cells were obtained by negative selection using the Dynabeads Untouched Mouse CD4 cell kit (ThermoFisher). Flow cytometry indicated >85% enrichment in all cases ...
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bioRxiv - Cell Biology 2022Quote: ... The complementary DNA oligos were annealed to generate a double-stranded oligo and cloned into the linearized pcDNA6.2-GW/EmGFP expression vector (using the BLOCK-iT Pol II miR RNAi Expression Vector kit, Invitrogen, K493600). As a negative control ...
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bioRxiv - Plant Biology 2022Quote: ... PRP39a was then transferred into the plant expression vector pB7FWG2 (Karimi et al., 2002) using the LR clonase II kit (Invitrogen). The p35S:XRN2-RFP ...
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bioRxiv - Plant Biology 2022Quote: ... each of the synthetic RNAi fragments was placed under the control of the CaMV-35S promoter in the binary destination vector pK2GW7 using Gateway LR Clonase II kit (Invitrogen). PhnsLTP1-RNAi construct was subcloned into a modified pRNA69 vector containing the Clarkia breweri linalool synthase (LIS ...
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bioRxiv - Genetics 2023Quote: ... were radio-labeled by random priming with P32-αdCTP (6,000 Ci/mmol; Perkin-Elmer) using the Decaprime II kit (Ambion Inc) and incubated with the membrane overnight at 65° C ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting PCR products were gel-purified using the Zymo gel extraction kit (ZymoResearch) and recombined into the Gateway DONR vector pDONR207 using BP Clonase II (Invitrogen) with overnight incubation at 25°C ...
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bioRxiv - Molecular Biology 2023Quote: ... ATI1 was amplified from oligo (dT) primed arabidopsis cDNA and subcloned into pDONR221 P3P2 entry vector with the BP clonase II kit (Invitrogen) and similarly ...
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bioRxiv - Developmental Biology 2023Quote: ... Additional probes have been synthesized from pCR-Blunt II-TOPO vectors after cloning of the respective sequences by using the Zero Blunt TOPO PCR cloning Kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The adapter-ligated RNA was reverse transcribed using primers provided in the Illumina kit and SuperScript II Reverse Transcriptase (Invitrogen). cDNA was amplified and indexed using the conditions provided in the Illumina protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The endocytosis marker:eGFP constructs were cloned into the Agrobacterium vector pIPKb001 (Himmelbach et al., 2007) using the Gateway LR Clonase II kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA constructs were inserted in TOPO II PCR-plasmids and using TOPO TA Cloning kit with chemically competent cells according to manufacturer’s protocol (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Mouse FM expression of IL1B and KC was measured by RT-qPCR using the SuperScript II kit (Invitrogen; Waltham, MA) and the Kapa SYBR Fast qPCR kit (Sigma-Aldrich ...