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Citations for Thermo Fisher :
901 - 950 of 10000+ citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The medium was ESTEM-HE medium during purification and changed to Human Melanocyte Basal Medium Kit (Gibco) or Melanocyte Growth Basal Medium-4 (Lonza ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was isolated from mouse aortas and human aortic SMCs with the PureLink RNA Mini Kit (ThermoFisher). Isolated RNA was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: Measurement of Aβ1-40 levels was performed using an Amyloid beta 40 Human ELISA Kit (Invitrogen, KHB3482). For further information please see Supplementary Material.
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from human lung ECs and mouse lung tissues using TRIZOL reagent kit (Invitrogen). The cDNA was generated using iScript cDNA synthesis kit (170-8891 ...
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bioRxiv - Molecular Biology 2023Quote: ... human primary T cells and DG-75 cells were electroporated using the Neon Transfection kit (ThermoFisher Scientific) at 1600 volts / 10 ms / 3 pulses ...
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bioRxiv - Developmental Biology 2019Quote: ... Dorothy genotyping was performed using the TaqMan™ Sample-to-SNP™ Kit using proprietary primers designed by the vendor targeting Nubp2:c:626T>A or Zbtb12:c:1061C>A (Thermo Fisher Scientific, Waltham, MA, USA). The Nubp2tm1a (Nubp2null ...
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bioRxiv - Cancer Biology 2021Quote: ... human BNIP3 (Hs00969291_m1) and human 18S (Hs99999901_s1) were purchased from Applied Biosystems. 18S served as an internal control ...
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bioRxiv - Microbiology 2022Quote: Human monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (83) ...
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bioRxiv - Immunology 2019Quote: ... First-strand cDNA generation was performed with 100ng of isolated total RNA using a SuperScript RT II kit (Invitrogen) and oligo-dT primer ...
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bioRxiv - Cell Biology 2019Quote: ... Total RNA was reverse transcribed with random hexamer primers using the Superscript II Reverse Transcriptase kit (ThermoFisher, 18064-022). qPCR was performed with SYBR Green PCR Master Mix (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... Two micro grams of total RNA was subjected to the reverse transcription using the Superscript II kit (Life Technologies). The relative gene abundance in the cDNA sample was examined by the quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2019Quote: ... 6 x 104 cells were transferred to a well of a 24-well plate in 500 μL of SFEM II with growth factors supplemented with CytoTune-iPS 2.0 Sendai Reprogramming Kit viral particle factors (Gibco) (Fujie et al. ...
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bioRxiv - Genetics 2019Quote: ... cDNAs were generated from 1µg of purified RNA with oligo (dT) primers using the SuperScriptTM II Reverse Trancriptase kit (Invitrogen).
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bioRxiv - Plant Biology 2021Quote: ... vector and subcloned into the pGWB602 or pGWB602Ω vectors (Nakamura et al, 2010) using LR Clonase II Kit (Invitrogen). The resulting pCPT7:LUC or 35S:HSFA1A constructs were transformed into the Agrobacterium tumefaciens GV3101 strain ...
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bioRxiv - Systems Biology 2020Quote: ... and in vitro transcription were performed according to the CEL-seq protocol (63) using Message Amp II kit (Ambion). Illumina sequencing libraries were subsequently prepared according to the CEL-seq2 protocol (63 ...
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bioRxiv - Cell Biology 2020Quote: ... Complementary DNAs (cDNAs) were synthesized from total RNA using Superscript II First-strand Synthesis kit (Invitrogen, Carlsbad, CA, USA) and quantified by real-time PCR using the IQ SYBR green supermix kit (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... the shRNA construct was obtained by cloning the sequence into pcDNA6.2-GW/EmGFP-miR plasmid using a microRNA (miR)-based expression vector kit (BLOCK-iT Pol II miR; Invitrogen), thereby creating an expression cassette consisting of the 5′ miR flanking region ...
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bioRxiv - Developmental Biology 2021Quote: ... 1μg of total RNA was used with oligoDT to synthesize cDNA libraries (Superscript II First-Strand Synthesis kit, Invitrogen). 2μg of cDNA was used in 28 cycles of PCR with PlatinumTaq (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.5μg of total RNA was used to synthesize cDNA libraries using oligoDT (Superscript II First-Strand Synthesis kit, Invitrogen). 1μL of cDNA was then used in a standard SYBR Green (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... Total RNA from each biological replicate was amplified using the MessageAmp II aRNA amplification kit (Ambion, Austin, TX, U.S.A.). Amplified RNA profiles were verified using the Experion analyser and Experion RNA standard-sense analysis kit (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... B cell magnetic sorting was performed using MagniSort Negative Selection Protocol II (MagniSort Mouse B cell Enrichment Kit, Affymetrix). Negatively selected cells were suspended in PBS and DNA extraction was performed.
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bioRxiv - Molecular Biology 2020Quote: ... DNA double-stranded probes were generated by PCR and radiolabeled with 32P-dATP using the Decaprime II kit (Ambion) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... H3.3 was cloned into pLEX_305-C-dTAG and p53 as well as RelA were cloned into pLEX_305-N-dTAG using Gateway LR clonase II Enzyme mix kit (Invitrogen). The sequences of the primers are listed below.
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bioRxiv - Cancer Biology 2021Quote: ... RNA was spectrophotometrically quantified and equal amounts were used for cDNA synthesis with the Superscript II RT Kit (Invitrogen). qPCR analysis was performed on an ABI Real Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... amplified on a cDNA template with respective primers (Supplementary Table S2) and DECAprime™ II labeling kit (Thermo Scientific) and [α-32P] dATP (SRP-203 ...
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bioRxiv - Plant Biology 2022Quote: ... The synthesized sequence was then recombined into the binary vector pSc4ActR1R2 using a Gateway LR Clonase II kit (Thermofisher) to create the plasmid pMM007 for stable wheat transformation ...
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bioRxiv - Microbiology 2023Quote: ... obtained from the Drosophila Genomics Resource Center (DGRC) by the LR clonase II enzyme mix kit (Thermo Fisher Scientific). UAS-spaid transgenic lines used in the fly genetics experiments (Fig ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µg of the total RNA was converted into cDNA using the SuperScript II Reverse Transcriptase kit (Invitrogen™). qPCR was performed on CFX384 Real- Time PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... The yeast two hybrid cDNA library was constructed from the mRNA of seedlings exposed to -1.2 MPa stress for 96 h according to the CloneMinerTM II cDNA library construction kit (Invitrogen) and then cloned into prey vector pDEST22 ...
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bioRxiv - Neuroscience 2023Quote: ... the DNA fragment encoding miRNA for Dsp (5’-TGC TGT TGA GAT GGA CAT CAA TGC ACG TTT TGG CCA CTG ACT GAC GTG CAT TGG TCC ATC TCA A-3’ and 5’-CCT GTT GAG ATG GAC CAA TGC ACG TCA GTC AGT GGC CAA AAC GTG CAT TGA TGT CCA TCT CAA C-3’) was subcloned into pcDNA6.2-GW-EmGFP-miR using BLOCK-iT Pol II miR RNAi Expression Vector Kit (Invitrogen). The EmGFP and Dsp miRNA fragments were amplified by PCR from this vector and inserted between the KpnI and EcoRV sites of the pAAV-EF1α-DIO EYFP vector (#27056 ...
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bioRxiv - Biophysics 2023Quote: ... These subsegments were inserted into the pCRBlunt II-TOPO vector using the Zero Blunt TOPO PCR cloning kit (Invitrogen). Point mutations in respective CaV1.3 subsegments were then generated using Quikchange II kit (Agilent) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Ten microliters of each of the total RNA sample was amplified prior to labelling following the manufacturer’s protocol (MessageAmp II aRNA Amplification kit, Ambion). We hybridized 3 μg of each labelled RNA sample to a single microarray slide ...
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bioRxiv - Neuroscience 2023Quote: Silencing of Fmr1 and Map1b has been performed using BLOCK-iT Pol II miR RNAi Expression Vector Kits (Invitrogen) and the RNAi Designer (Invitrogen) ...
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bioRxiv - Pathology 2023Quote: ... were incubated with 20 μg/ml DQ gelatin fluorescein conjugate for 2 h at 37°C according to kit instructions (EnzChek Gelatinase/Collagenase Assay Kit, E-12055, Molecular Probes, Inc). The fluorescence generated by hydrolysis of the added substrate was captured by ZEISS Axioscan 7 (ZEISS Microscopy ...
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bioRxiv - Molecular Biology 2020Quote: ... Human transferrin-568 (ThermoFisher) was added at 10μg mL−1 concentration in serum free media and incubated at 37°C to allow for internalization ...
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bioRxiv - Immunology 2021Quote: ... then anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 50 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Cancer Biology 2022Quote: Probes Hs00171064_m1 (human, ThermoFisher), Mm00440280_g1 (mouse ...
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bioRxiv - Bioengineering 2022Quote: ... Primary human hepatocytes (Gibco) were seeded in the top channel at a density of 3.5 x 106 cells/mL using complete hepatocyte seeding media ...
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bioRxiv - Immunology 2020Quote: ... or anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 25 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... or human IgG (Invitrogen) as control ...
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bioRxiv - Bioengineering 2022Quote: ... and human fibronectin (Gibco) at 50 μg/mL in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... human (ThermoFisher Scientific, 902927). Analysis was performed with Transcriptome Analysis Console 4.0 software (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... then anti-human (Invitrogen) horseradish peroxidase-conjugated antibodies were diluted 1:5,000 and 50 μL added to each well and incubated at 37°C for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% human IgG (Invitrogen) in PBS] followed by incubation with primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... human plasma (ThermoFisher, #33016015); Laminin 111 ...
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bioRxiv - Immunology 2023Quote: ... A Human ProcartaPlexTM (Invitrogen) immunoassay was additionally used to detect 45 human cytokines ...
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bioRxiv - Immunology 2024Quote: ... Anti-Human-HRP (Invitrogen) was diluted 1:5000 in 1% BSA/PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... CD36 (Human tissue Thermofisher: PA1-16813 1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Alexa Fluor 568 (MCN1 terminal) and Alexa Fluor 488 (LG) potassium salts (Thermo Fisher Scientific, Waltham, MA, USA) were diluted in 200 mM KCl and loaded in the electrode tip ...
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bioRxiv - Cancer Biology 2020Quote: ... Full-length EWS/FLI and mutants (all containing amino-terminal 3xFLAG-tags) were cloned into pMSCV-Hygro (Invitrogen) with sequence details provided in Additional file 1 ...