Labshake search
Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: 1 µl Cas9 protein (5 µg/µl, Thermo Fisher) and 100 pM of synthetic guide RNA (Tspan8 guide sequence 5’ – 3’ ...
-
bioRxiv - Cell Biology 2022Quote: ... corresponding proteins were expressed from modified pPROEX-1 (Invitrogen)-based plasmids as N-terminal 6 x His-TEV fusion proteins ...
-
bioRxiv - Biophysics 2022Quote: ... 1 % BSA blocked protein G agarose (Thermo Scientific 20398) and anti-CD3ε (UCHT1 clone ...
-
bioRxiv - Microbiology 2023Quote: ... 1:150 diluted Bacterial Protein Extraction Reagent (Thermo Fisher); and 1 mg mL-1 of 2-nitrophenyl-β-galactopyranoside (Sigma)) ...
-
bioRxiv - Systems Biology 2021Quote: ... MDA-MB-231 human breast cancer cells were cultured in DMEM/F12 (1:1) media52 (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2022Quote: ... and incubated with anti-mouse/human CD309 (Flk-1/VEGFR2)-APC (Thermofisher, 17-5821-81; 1:100) for 30 minutes at 4°C ...
-
bioRxiv - Biophysics 2020Quote: ... Samples were heated to 90°C over 1 hr and then slowly cooled overnight to 4 °C (−1°C per 20 minutes) using a Thermocycler (ProFlex PCR System, ThermoFisher). Refolded samples were resuspended through pipetting and combined into a fresh 1.5 mL microfuge tube ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated with primary antibodies in 10% NGS/NDS in PBST over night at 4°C following 1h incubation at 24°C with appropriate Alexa488/568 coupled secondary antibodies (1:1000, Invitrogen). Cell nuclei were stained with DAPI and final images were acquired using Leica TCS SP8 confocal microscope (Leica Microsystems ...
-
bioRxiv - Immunology 2019Quote: ... Briefly, cDNA were synthetized in 10 μl (50°C 1 h, 72°C 10 min) using SuperScript III Enzyme (ThermoFisher). After RNase H treatment ...
-
bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from the primary fibroblast cultures derived from patient PFS and 4 healthy unrelated gender-matched controls (3 homozygous for wild-type C allele at rs324420 and 1 heterozygous C/A) using the PureLink RNA Micro Kit (Invitrogen) and run by Eurofins on the human Clariom D transcriptomic array (Thermo Fisher ...
-
bioRxiv - Cell Biology 2023Quote: ... 40 cycles of 95°C for 1 s and 60°C for 20 s on the QuantStudio 5 Real-Time PCR System (ThermoFisher). Relative quantification of each gene was done by QuantStudio Design&Analysis v1.5.1 software (ThermoFisher ...
-
bioRxiv - Plant Biology 2023Quote: ... for 40 cycles (15 seconds at 95°C, 1 minute at 60°C) in a QuantStudio 6 Flex instrument (ThermoFisher). Percentage of input was calculated as 100* e(CtIN – log2(DF ...
-
bioRxiv - Biochemistry 2023Quote: ... The c-Myc tag labeling solution was prepared by a 1:100 dilution of c-Myc Alexa Fluor 488 conjugate (ThermoFisher) in PBS containing 0.2% BSA ...
-
bioRxiv - Microbiology 2023Quote: ... The epidermis was peeled off and discarded and the dermis was minced into small pieces and digested at 37°C for 1 h following 4°C for overnight using collagenase IV (17104019, Gibco). The resulting cell suspension was filtered through a 70 μm cell strainer (BD Falcon) ...
-
bioRxiv - Biochemistry 2024Quote: ... The RT reactions were then carried out at 37 °C for 1 hour followed by heating at 80 °C for 10 minutes to inactivate the RT (for SSIII, Invitrogen, 18080093 ...
-
bioRxiv - Genomics 2020Quote: ... Chromatin was pre-cleared with 1:1 protein A:protein G Dynabeads (Life Technologies) and immunoprecipitation was carried out with H3K27ac-bead complexes at 4°C overnight ...
-
bioRxiv - Genetics 2020Quote: ... 50 uL of a 1:1 mix of Protein A (Cat #10008D, Invitrogen) and Protein G Dynabeads magnetic beads (Cat #10004D ...
-
bioRxiv - Pathology 2024Quote: ... si-TGF-beta activated kinase 1 binding protein 1 (TAB1, Thermo Fisher Scientific) was transfected into hDPCs using Lipofectamine 3000 (Thermo Fisher Scientific) ...
-
bioRxiv - Developmental Biology 2021Quote: ... ESCs were dissociated with TrypLE Express for 5 mins at 37°C and induced into EpiLCs by addition of human recombinant basic fibroblast growth factor (bFGF) (Invitrogen, 13256-029) and activin A (Peprotech ...
-
bioRxiv - Biophysics 2021Quote: ... After washing thrice with PBS-0.1% Tween 20 the membrane was probed with primary antibody which is a rabbit monoclonal anti human lamin A+C antibody (Thermo Scientific, USA) and Stabilized Peroxidase Conjugated secondary Goat Anti-Rabbit (H+L ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were pulsed in starvation buffer supplemented with 0.1% w/v bovine serum albumin at 4 °C for 40 min with 50 ng/mL human recombinant EGF (Thermo-Fisher, Gibco™, PHG0311L) to allow ligand bind to the EGFR ...
-
bioRxiv - Microbiology 2022Quote: ... falciparum B11 line (Perrin et al., 2018) was maintained at 37° C in human RBCs in RPMI 1640 containing Albumax II (Thermo Fisher Scientific) supplemented with 2 mM L-glutamine ...
-
bioRxiv - Immunology 2019Quote: ... cells were cultured for 14 days in a humified CO2 incubator at 37°C in complete culture medium with Dynabeads® Human T-Activator CD3/CD28 beads (ThermoFisher Scientific) (25µl/well ...
-
bioRxiv - Neuroscience 2021Quote: hiPSCs were previously generated from healthy adult human dermal fibroblast lines from a 32-year-old female from Invitrogen (C-013-5C), as described before (31) ...
-
bioRxiv - Cell Biology 2021Quote: ... AlexaFluor488-labeled human transferrin was dissolved in PBS 5 at mg/ml and stored at 4°C (Thermo Fisher, ref. no. 13342). TexasRed-labelled neutral 3’000 and 40’000 Da dextran was dissolved in PBS at 10 mg/ml and stored at -20°C (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2022Quote: Human K562 cells (ATCC CCCL-243) were grown at 37°C and 5% CO2 in RPMI 1640 medium +GlutaMAXTM(Gibco 61870-036) supplemented with 10% FBS (HyClone SH30071 ...
-
bioRxiv - Microbiology 2024Quote: ... falciparum B11 line (Perrin et al., 2018) was maintained at 37°C in human RBCs in RPMI 1640 containing Albumax II (Thermo Fisher Scientific) supplemented with 2mM L-glutamine ...
-
bioRxiv - Microbiology 2023Quote: ... falciparum B11 line (Perrin et al., 2018) was maintained at 37◦ C in human RBCs in RPMI 1640 containing Albumax II (Thermo Fisher Scientific) supplemented with 2 mM L-glutamine ...
-
bioRxiv - Bioengineering 2023Quote: ... Cryopreserved hiPSC-derived endothelial cells were thawed in a water bath at 37 °C and transferred into a 15 mL tube containing 7 mL of Human Endothelial SFM medium (11111044; Thermo Fisher Scientific). The cells were centrifuged at 300 x g for 3 min and the pellet was resuspended in Human Endothelial SFM complete medium ...
-
bioRxiv - Molecular Biology 2021Quote: ... cerevisiae EKY3/pYES2-TOP1 induced for expression of wild-type human TOP1with the PrepEase Histidine-Tagged Protein Purification Midi Kit – High Specificity (from Affymetrix) according to the manufacturer’s procedures ...
-
bioRxiv - Genomics 2020Quote: ... Both cell lines were maintained in culture vessels coated with a matrix of Vitronectin human recombinant protein (ThermoFisher Scientific, A14700) and batch fed daily with mTeSR (STEMCELL Technologies ...
-
bioRxiv - Bioengineering 2021Quote: All experiments used a previously described research cell bank of T-REx-293 human embryonic kidney (HEK) cells stably expressing the tetracycline repressor protein (ThermoFisher) (4) ...
-
bioRxiv - Neuroscience 2021Quote: ... The tandem mass spectra were searched using SEQUEST 80 search algorithms against a Uniprot database (human 2019_07 release for 293T CoIP and mouse 2019_08 release supplemented with human TDP-43 protein sequence for mouse CoIP) through the Proteome Discoverer platform (version 2.5, Thermo Scientific). The search parameters included a maximum of two missed cleavages ...
-
bioRxiv - Cell Biology 2021Quote: MS/MS spectra were searched against a Swiss-Prot human database containing 20,341 reviewed protein entries using Mascot algorithm (Matrix Science) via Proteome Discoverer 2.2 (PD, Thermo Fisher Scientific) and were processed as previously described (10) ...
-
bioRxiv - Cell Biology 2021Quote: Human ProtoArray® Microarray (v5.0) containing 9483 unique human proteins expressed as GST-fusion in insect cells were purchased from Invitrogen. Arrays were blocked in 4°C cold blocking buffer (50 mM HEPES pH 7.4 ...
-
bioRxiv - Cell Biology 2022Quote: The human cDNA encoding zDHHC2 was codon optimised for protein expression in insect cells and synthesized by GeneArt technology (Invitrogen). It was inserted with N and C-terminal tags (His6 and StrepII ...
-
bioRxiv - Cell Biology 2022Quote: ... with Essential 8 Supplement (1X) in culture vessels coated with 5mg/ml vitronectin (Recombinant Human Protein; Life Technologies, London, UK). Spontaneous differentiation medium was Knockout DMEM ...
-
bioRxiv - Microbiology 2020Quote: We purified polyclonal IgG from human plasma samples using Pierce™ Protein G Ultra Link™ Resin (Thermo Fisher Scientific) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: ... Acquired DDA .raw files were searched against the human UniProtKB/SwissProt database (v2012_07) using Byonic (Protein Metrics) embedded in Proteome Discoverer (ThermoFisher Scientific) to obtain peptide sequence information ...
-
bioRxiv - Cell Biology 2021Quote: ... WT human PRRT2 (gene ID: 112476) was synthesized as N-terminal HA tag fusion protein (Genewiz) in pcDNA3 vector (Thermofisher) and single point mutants were generated on this matrix by standard molecular biology techniques.
-
bioRxiv - Immunology 2020Quote: ... Mass spectrometry derived data was searched against Human RefSeq 81 protein database in Proteome Discoverer 2.1 (Thermo Scientific, Bremen, Germany) using SEQUEST and Mascot (version 2.5.1 ...
-
bioRxiv - Biochemistry 2020Quote: ... serum reactivity to the Spike protein was measured using an HRP-conjugated goat anti-human IgG (H+L) (Invitrogen, 31410) at a 1:3000 dilution for 1 hour ...
-
bioRxiv - Neuroscience 2022Quote: ... 50 and 35µg of proteins (for cell lysates, mouse brains and human cortices, respectively) were added with 1x Sample buffer (Thermo Fisher) and 1x Sample Reducing Agent (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2022Quote: ... They were adapted and then cultured in feeder-free conditions on culture dishes coated with the Vitronectin Recombinant Human Protein (ThermoFisher) in mTeSR1™ medium (StemCell Technologies ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Syncytin1 was recoded based on the amino acid sequence from the The Human Protein Atlas and ordered as a gBlock (ThermoFisher). PCR reactions were performed using a high-fidelity polymerase (KOD-Xtreme ...
-
bioRxiv - Neuroscience 2022Quote: ... HeLa cells were transfected with green fluorescent protein (GFP)-tagged human NCKAP1 cDNA or NCKAP1 shRNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: Recombinant human SHBG (ProSpec, Israel) was directly labeled with Alexa Fluor-555 using a protein conjugation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2024Quote: ... Recombinant protein with N-terminal 9x-His and GST tag was produced by amplifying the coding sequences of NRL5 and NRL5P335L and cloning into the expression vector pT7CFE1-NHIS-GST-CHA (Human In Vitro Protein Expression Vector, ThermoFisher Scientific ...
-
bioRxiv - Systems Biology 2024Quote: All RAW files acquired from TMT-MS of all cases were searched against a human reference protein database using the Proteome Discoverer suite (version 2.4, ThermoFisher Scientific). MS2 spectra were searched against the UniProtKB human proteome database containing Swiss-Prot human reference protein sequences (20,338 target proteins downloaded in 2019) ...
-
bioRxiv - Neuroscience 2024Quote: Organoid culture supernatants and lysates were analyzed for secreted proteins using single- and multiplexed Human ProcartaPlex™ Panel (Thermofisher Scientific), following previously documented procedures.37,38 The plates were read using the Luminex MAGPIX xMAP instrument and xPONENT® software ...