Labshake search
Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 7 CHLORO 2 ETHYL 1H INDENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... AlexaFluor 568 goat anti mouse (Invitrogen, A11004, 7 RRID:AB_2534072, 1:2000), AlexaFluor 568 anti rabbit (Invitrogen ...
-
bioRxiv - Cell Biology 2024Quote: ... and CellTracker Blue (4-chloromethyl- 6.8- difluoro- 7- hydroxycoumarin; CMF2HC; Invitrogen), respectively ...
-
bioRxiv - Cell Biology 2024Quote: Real-time search (RTS)7 was executed in XCalibur (ThermoFisher Scientific). RTS was performed on MS2 scans using a concatenated forward and reverse human reference proteome based on the FASTA file available from Uniprot (updated December 21 ...
-
bioRxiv - Immunology 2024Quote: ... the supernatant was desalted using Zeba 7 kDa spin columns (ThermoFisher) to remove free payload ...
-
bioRxiv - Microbiology 2024Quote: ... and 7 μL of antifade reagent (SlowFadeTM Gold, Invitrogen ref S36937) was added in each well ...
-
bioRxiv - Cell Biology 2024Quote: ... Potassium chloride (7447-40-7-500G) was obtained from Fisher Scientific. Paraformaldehyde (16% ...
-
bioRxiv - Immunology 2024Quote: ... on a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific). The primers are listed in Supplemental Table 3 ...
-
bioRxiv - Immunology 2024Quote: ... with 20 μg/ml of 7-aminoactinomycin D (7AAD, Invitrogen A1310) in perm/wash (BD Biosciences) ...
-
bioRxiv - Immunology 2024Quote: ... CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher, C10423) was added to cultures at 20 μM and incubated for 30 min at 37°C ...
-
bioRxiv - Systems Biology 2021Quote: The relative mitochondrial transmembrane potential (ΔΨm) was measured using the membrane-potential-dependent fluorescent dye TMRE (Tetramethylrhodamine, Ethyl Ester, Perchlorate) (Molecular Probes, Thermo Fisher Scientific)[87] ...
-
bioRxiv - Cancer Biology 2022Quote: ... with or without N-ethyl-N-nitrourea 10μg/ml or 50μg/ml and treated 2h with 1mg/ml Collagenase type IV (Gibco by life technologies ref 17104-019).
-
bioRxiv - Molecular Biology 2023Quote: ... and brain tissues were weighed and homogenized at a density of 140 mg of dry tissue per milliliter in ethyl acetate / isopropanol (4:1) using a Bead Mill 4 homogenizer (Thermo Fisher Scientific, Waltham MA) and 2-mL pre-filled polypropylene microtubes (2.8 mm ceramic beads ...
-
bioRxiv - Microbiology 2020Quote: ... Four to six-day old mated female mosquitoes were allowed to feed for 1h on approximately 108 PFUs of SINV (TE12-untagged) containing citrated rabbit blood (Fisher Scientific DRB030) supplemented with 1mM ATP (VWR ...
-
bioRxiv - Microbiology 2020Quote: ... 30,000 cells/well were cultured at 37°C in 5% CO2 as a monolayer on coverslips (d = 12 mm; Paul Marienfeld GmbH & Co. KG) in 24 well plates (#08-772-1H, Thermo Fisher Scientific) with DMEM ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were blocked in blocking buffer (1% BSA, PBST) for 1h at room temperature and incubated with primary antibody LC3A/B (Thermo Scientific, USA) overnight at 4 °C ...
-
bioRxiv - Bioengineering 2021Quote: ... Washed samples were incubated for 1h at RT with the secondary antibody (1:200 Alexa Fluor 647 goat derived anti-rabbit antibody, Thermo Fisher Scientific). Then ...
-
bioRxiv - Neuroscience 2020Quote: ... for 2h at RT followed by a 1h incubation with 1:200 secondary goat anti-rabbit antibody Alexa 647 (Thermo Fisher, A32733) and DAPI to visualize cell nuclei ...
-
bioRxiv - Microbiology 2021Quote: ... Cells infected with YFV were incubated with the pan-flavivirus anti-Env 4G2 antibody for 1h at 4°C and then with Alexa 488 anti-mouse IgG secondary antibodies (Thermo Fisher, #A28175) for 45 min at 4°C in the dark ...
-
bioRxiv - Cancer Biology 2020Quote: ... the flasks were shaken at 37°C for 1h at 170 rpm in an orbital shaker (Max Q 4000; Thermo Fisher Scientific). The supernatant containing microglia was then collected [36].
-
bioRxiv - Neuroscience 2020Quote: ... for 1h at room temperature (or overnight at 4°C) and the unbound dye was removed using spin columns (Thermofisher, Waltham, MA). The labeling efficiency was estimated using absorbance values (488 or 555 or 660 nm/280 nm ...
-
bioRxiv - Microbiology 2022Quote: ... Bound primary antibody was then labeled for 1h at room temperature using Alexa488 goat anti-mouse IgG secondary antibody (Invitrogen, OR, USA) at a 1:750 dilution in PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... samples were washed three times and incubated 1h at room temperature with secondary antibodies: Alexa Fluor Plus 488 goat anti-Rabbit (A32731, Thermo Fisher Scientific) and Alexa Fluor Plus 546 donkey anti-goat (A11056 ...
-
bioRxiv - Neuroscience 2019Quote: ... Cells were washed and incubated for 1h with Alexa Fluor 488-conjuated antibody to rabbit IgG (A-21206, 1:100, Thermo fisher scientific). Images of green fluorescence were assessed by laser confocal fluorescence microscopy.
-
bioRxiv - Microbiology 2019Quote: ... pneumophila antisera (1:20,000) for 1h at 37°C in PBS containing 4% BSA followed by anti-rabbit IgG Alex Fluor 488 (Invitrogen, 1:500) for 1h at 37°C in PBS containing 4% BSA ...
-
bioRxiv - Microbiology 2021Quote: ... The beta-propiolactone was inactivated by incubation at 37C for 2 hours and the inactivated virions were pelleted by ultracentrifugation at 25000g for 1h at 40C and protein concentration was determined by BCA assay (Thermo Fisher Scientific).
-
Overexpression of HLA class I predominantly on alpha cell in at risk individuals for type 1 diabetesbioRxiv - Immunology 2020Quote: ... for 1h at room temperature (RT) followed by detection using polyclonal goat anti-mouse IgG-Alexa Fluor 647 (Life Technologies, 1:1000) for 30 min at RT ...
-
bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
-
bioRxiv - Cancer Biology 2022Quote: ... in the hybridization buffer for 1h at RTThe sections were washed with PBS and mounted with SlowFade Gold Antifade Mountant (Thermo Fisher Scientific). After each imaging cycle ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein lysates were pre-cleared 1h at 4°C on a rotating wheel with 50μl of previously washed Dynabeads Protein G (Invitrogen, Thermo Fisher Scientific), to avoid aspecific bindings ...
-
bioRxiv - Cancer Biology 2022Quote: ... diluted at 1/50 or 1/150 followed by an incubation for 1h at room temperature with a secondary goat Alexa FluorTM 488 antibody (ThermoFisher Scientific, #A11029) diluted at 1/1000 ...
-
bioRxiv - Biochemistry 2023Quote: ... the slides were incubated with primary antibodies in PBS containing 5% BSA for 2 h and then with the following secondary antibodies for 1h at room temperature: Donkey Anti-Rabbit Alexa 488 (1:1000; Invitrogen; A21206, 2376850) and Donkey Anti-Chicken Alexa 594 (1:1000 ...
-
bioRxiv - Genetics 2023Quote: ... 1 μL was spotted onto a C+Y-10% polyacrylamide pad (incubated twice for 1h in C+Y medium supplemented with daptomycin when applicable) inside a Gene Frame (Thermo Fisher Scientific) sealed with a cover glass ...
-
bioRxiv - Neuroscience 2023Quote: ... 10% Donkey Serum, 1% Triton-100, 1h) followed by primary (overnight, 4°C) and secondary antibodies (1-2h, Jackson ImmunoResearch or ThermoFisher Scientific) incubation ...
-
bioRxiv - Cell Biology 2023Quote: ... primary antibodies in 10% GS in 1xPBS overnight at 4°C followed by a 1h incubation with anti-mouse (A11003, Invitrogen, 1:800) secondary antibody in 10% GS in 1xPBS at room temperature ...
-
bioRxiv - Cancer Biology 2023Quote: ... Secondary antibody incubation with anti-rabbit-HRP and anti-mouse-HRP was done at room temperature for 1h in 5% milk in TBS-T and the signal developed using an ECL mixture (Life Technologies, 32132). For a detailed list of antibodies see Supplementary Table 2 ...
-
bioRxiv - Microbiology 2023Quote: ... The cell membrane fraction was isolated from the lysates by performing ultracentrifugation at 66000g for 1h (Sorvall WX Ultra 80 Centrifuge, Thermo Fisher Scientific). After resuspending the obtained pellet with PBS ...
-
bioRxiv - Bioengineering 2023Quote: ... stained with primary antibody for 1h at 37° C and labeled against F-actin (1:60 in PBS Alexa Fluor® 488 Phalloidin-Invitrogen, # A12379) and DNA (NucBlue ...
-
bioRxiv - Cancer Biology 2023Quote: 250’000 Jurkat cells or purified T cells were seeded onto a 24-well plate and transduced at an MOI of 5 via spin-infection for 1h at 800xg and 32°C in 450 µl RPMI-1640 medium (Gibco, #42401-018) containing 50 µl of 100-fold concentrated lentiviruses ...
-
bioRxiv - Microbiology 2024Quote: ... then the following secondary antibodies were diluted 1:500 in blocking buffer and incubated for 1h with the cells: anti-rabbit DyLight 448 (Thermo Scientific, 35552), anti-rabbit DyLight 550 (Thermo Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... and incubated 1h at RT in the dark with a secondary antibody solution containing goat anti-mouse Alexa 594 (A11032, Invitrogen, Fisher Scientific) fluorescent-coupled antibody diluted to 1:1000 in PBS-T solution ...
-
bioRxiv - Plant Biology 2019Quote: ... using a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). The expression levels were calculated with the 2−ΔΔCt method ...
-
bioRxiv - Neuroscience 2021Quote: ... 7 million primary neurons were plated on 60 mm culture dishes (ThermoFisher) and cultured for 7 days ...
-
bioRxiv - Cell Biology 2019Quote: MSFs were transfected with mRuby-Lifeact-7 using Lipofectamine 3000 (Life Technologies) 18 h after seeding into stretch chambers ...
-
bioRxiv - Cell Biology 2020Quote: ... [7] in the presence of 1X Halt protease inhibitor cocktail (Thermo Scientific) and protein quantified using the DC BioRad assay ...
-
bioRxiv - Cell Biology 2020Quote: ... The samples were run on the ViiA 7 thermocycler (Thermo Fisher Scientific) using standard cycling parameters provided by the manufacturer ...
-
bioRxiv - Molecular Biology 2021Quote: ... and ran on a ViiA 7 Real-Time PCR System (ThermoFisher Scientific), with a 15-second 95°C denaturation step and a 1-minute 60°C annealing/extension step for 40 cycles ...
-
bioRxiv - Microbiology 2021Quote: ... and 1 mM TCEP) with Zeba 7 kDa MWCO spin columns (ThermoFisher), re-quantified by A280 absorbance ...
-
bioRxiv - Developmental Biology 2021Quote: ... Plates were run on a ViiA-7 Real-Time PCR system (ThermoFisher), and CT values were auto-determined by the ViiA-7 software ...
-
bioRxiv - Immunology 2022Quote: ... on an ABI ViiA 7 Real-Time PCR system (Thermo Fisher Scientific). Forward and reverse primer sets were designed using NCBI Primer-Blast software and purchased from Integrated DNA Technologies ...
-
bioRxiv - Cell Biology 2022Quote: ... and differentiated into macrophages for 7 days in RPMI (Gibco, Life Technologies) supplemented with 5% fetal calf serum (FCS ...