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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and 4′,6-diamidino-2-phenylindole (DAPI; 2 μg/ml; Invitrogen) mixed in 1% BSA in PBS ...
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bioRxiv - Neuroscience 2023Quote: Cell viability was assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Cat. No. M6494, ThermoFisher Scientific, Waltham, MA, USA) to differentiate the range of toxic and nontoxic palmitic acid concentrations ...
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bioRxiv - Immunology 2020Quote: ... CB (1:1) and buffy coats (1:2) were diluted with sterile 1xPBS (Gibco by Life Technologies, California, USA) and MNCs were isolated by density gradient centrifugation (Biocoll ...
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bioRxiv - Immunology 2020Quote: ... CB (1:1) and buffy coats (1:2) were diluted with sterile 1xPBS (Gibco by Life Technologies, California, USA) and MNCs were isolated by density gradient centrifugation (Biocoll ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we combined 1 µg of total RNA + 2 µl 1:100 ERCC RNA Spike-in Mix #1 (Invitrogen #4456740).
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we combined 1 µg of total RNA + 2 µl 1:100 ERCC RNA Spike-in Mix #1 (Invitrogen #4456740). Libraries were prepared using the Kapa RNA HyperPrep Kit with RiboErase (Roche #08098131702) ...
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bioRxiv - Bioengineering 2024Quote: ... diluted in a weight-ratio of 13:1:1:1:2 in 1.5 mL of Opti-MEM (31985062, Gibco). This solution was added to the 10 cm dish in a drop-wise pattern when the cells had reached ∼60% confluency ...
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bioRxiv - Cell Biology 2021Quote: ... cells were pulsed with 10µM 5-Ethynyl-2’deoxyuridine (EdU) for 2 hours following manufacturer’s instructions (Click-iT® EdU Alexa Fluor® Imaging Kit, Life Technologies). Cells were subsequently fixed and stained with Hoechst 33342 ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Neuroscience 2022Quote: ... and 12 old (21 months) male C57BL/6 animals (combined over 2 independent experiments) were intraperitoneally injected with 5-ethynyl-2’- deoxyuridine (EdU) (Fisher Scientific, A10044) (resuspended in PBS at 5 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were loaded onto a 2 cm pre-column (C18, 5 μm, 100 A°, 100 μ, 2 cm Nano-viper column # 164564, Thermo Scientific) at 5 μl/minute flow rate using loading pump for about 5 minutes and then resolved the peptides on a 50 cm analytical column (C18 ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Genetics 2020Quote: ... Throughout the neural conversion and patterning phase (D0-18) the neuroepithelial layer was enzymatically dissociated twice (at D4-5 and D10-12) using dispase (GIBCO, 1 mg ml-1).
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bioRxiv - Plant Biology 2022Quote: ... total soluble proteins were extracted from 50 mg of plant materials grown as indicated previously with 100 μL 2 x Laemmli buffer supplemented with 1% Phosphatase Inhibitor Cocktail 2 (Thermo Fisher Scientific Inc., 78430). Proteins were denatured for 10 min at 95°C and separated on 10% or 8% SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... F(ab’)2 goat anti-mouse Alexa Fluor 350 (Molecular Probes 1:1000) and F(ab’)2 goat anti-mouse Alexa Fluor 633 (Molecular Probes ...
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bioRxiv - Neuroscience 2021Quote: ... The 2° antibody used was anti-chicken Alexa 488 (1:500, Life Technologies). Confocal image stacks were acquired on a Leica SP8 ...
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bioRxiv - Neuroscience 2021Quote: ... We used a MAP-2 polyclonal antibody (PA5-17646 Invitrogen, 1:1000 dilution) to stain for dendrites ...
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bioRxiv - Immunology 2022Quote: ... 1-2 million PBMCs were plated into 12 well plate (Nunc, Thermo Fisher) at 37°C and 5% CO2 and allowed to adhere for two hours in 1 mL of RPMI 1640 (Hyclone ...
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bioRxiv - Immunology 2022Quote: ... 1-2 million PBMCs were plated into 12 well plate (Nunc, Thermo Fisher) at 37°C and 5% CO2 and allowed to adhere for two hours in 1 mL of RPMI 1640 (Hyclone ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... gene using RNase P probe purchased from Applied Biosystems and RNase P primer 1 and RNase P primer 2 purchased from Invitrogen. The final concentration of primers was 500 nM each and probe was 250 nM ...
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bioRxiv - Immunology 2020Quote: ... mouse CD68 (Thermo Fisher Scientific, USA, 1:100, 2 h at 37°C) for macrophages and pDC’s were identified by co-staining of PE conjugated mouse anti-human CD123 (BD Biosciences ...
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bioRxiv - Bioengineering 2021Quote: ... were maintained in a 2:1 mix of FreeStyle293:Expi293 Expression medium (ThermoFisher) and grown at 37 °C and 8% CO2 while shaking at 120 rpm ...
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bioRxiv - Bioengineering 2021Quote: DNA sizes were first confirmed by running 1-2% Agarose E-Gel (Invitrogen). Then ...
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bioRxiv - Microbiology 2021Quote: ... containing 100µl Schneider’s media (Experimental Replicates 1 and 2) or RPMI (ThermoFisher Scientific) (Experimental Replicates 3 and 4 ...
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bioRxiv - Neuroscience 2020Quote: ... 2 mM L-glutamine and 1% MEM non-essential amino acids (ThermoFisher Scientific). Cells were collected 72 h after transfection with 125 pmol Stealth siRNA using Lipofectamine® 2000 (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant aFGF/FGF-1 and bFGF/FGF-2 were purchased from Thermo Fisher Scientific (13241-103 and PHG0024) ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (1:10,000, Invitrogen) for 3 min and eventually coverslips were mounted with Dako mounting kit (Fluka) ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... 2 mM GlutaMAX and 1 % antibiotic-antimycotic (all Thermo Fisher Scientific, Epsom, UK). SH-SY5Y cells stably expressing YFP-alpha-synuclein were obtained by lentiviral transfection using 3rd generation lentiviruses (Addgene constructs ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 mmol/l 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Gibco, Germany #15630056), 2.5 ng/ml human fibroblast growth factor-basic (hFGF2 ...
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bioRxiv - Microbiology 2020Quote: ... 1 mL of culture was mixed with 2 volumes of RNAlater (Invitrogen, USA) for five minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Isolated corneas were fixed (2% PFA, 1 hour) and stained (Click-iT, Invitrogen) according to the manufacturer’s instructions followed by wholemount staining protocol (described above ...
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bioRxiv - Cell Biology 2021Quote: ... DAPI was used (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, 1:10 000, Invitrogen).
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bioRxiv - Cancer Biology 2022Quote: ... 1:500) and 4,6-diamidino-2-phenylindole (DAPI; ThermoFisher Scientific #D1306, 300 nM) for 1 hour at RT ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated with diamidino-2-phenylindole (DAPI; 1:1000; Thermo Fisher Scientific) before being mounted with Vectashield HardSet (Vector Laboratories ...
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Cell-Type Specific Profiling of Histone Post-Translational Modifications in the Adult Mouse StriatumbioRxiv - Neuroscience 2022Quote: ... anti-H3K27me3 (Antibody 1: Active Motif, 39055; Antibody 2: Thermo Fisher, MA5-1198) or IgG (Epicypher ...
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bioRxiv - Microbiology 2022Quote: ... and 2% agar (Agar bacteriological no. 1, Oxoid, Thermo Fisher Scientific, Waltham, USA). Lactobacillus brevis and P ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA (1-2 µg) was reverse transcribed using SuperScript IV reverse transcriptase (Invitrogen). Expression of specific mRNAs was determined with a LightCycler 480 (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 µg non-integrating PBase transposase vector and 2 µl Lipofectamine-2000 (Invitrogen) were incubated for 20 min in 100 µl DMEMF12 (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclear counterstaining was performed using 4,6-diamidino-2-phenylindole (DAPI,1:6000, Invitrogen).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and calnexin antibody (1:500 Thermo Fisher Scientific Inc., cat.no. 10427-2-AP) were used for immunoblotting.
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bioRxiv - Microbiology 2021Quote: ... mice were intravenously injected with Hydrazide AlexaFluor633 (2 mg.kg− 1, ThermoFisher Scientific, #30633) to label arteriole walls ...
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bioRxiv - Microbiology 2022Quote: DAPI (diamidino-2-phenylindole) Solution (1 mg/mL) (Thermo Fisher Scientific, catalog#: 62248)
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bioRxiv - Developmental Biology 2020Quote: ... 2 μL ERCC spikes (AM4456740) (diluted 1:40,000 in UltraPure H2O (InVitrogen 10977023) containing carrier tRNA (AM7119 ...
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bioRxiv - Cell Biology 2020Quote: ... 1% P/S and 50 μM 2-mercaptoethanol (Gibco, via Thermo Fisher Scientific), and 20 ng/ml recombinant murine granulocyte-macrophage colony-stimulating factor (mGM-CSF ...
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bioRxiv - Cell Biology 2020Quote: ... 1% P/S and 50 μM 2-mercaptoethanol (Gibco, via Thermo Fisher Scientific), and 20 ng/ml recombinant murine granulocyte-macrophage colony-stimulating factor (mGM-CSF ...
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bioRxiv - Molecular Biology 2020Quote: ... (2) Beads were resuspended in 1% SDS and treated with proteinase K (Invitrogen) for 30 minutes at 37° C ...
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bioRxiv - Plant Biology 2021Quote: ... 1-2 μg of total RNA treated with DNase I (Thermo Fisher Scientific) was reverse transcribed using either the High-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Immunology 2020Quote: ... 1% (v/v) Hepes buffer and 0.05 M of 2-Mercaptoethanol (all Gibco) supplemented with 30% conditioned medium from mouse macrophage colony stimulating factor (M-CSF)-producing L929 cells ...
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bioRxiv - Cell Biology 2020Quote: ... Eps8L2 siRNA #1 and Eps8L2 siRNA #2 contain Silencer Select modifications (Life Technologies), Ctdnep1 siRNA #2 and Nesprin2G siRNA were from Genecust Europe ...
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bioRxiv - Biochemistry 2021Quote: ... and 1:4 pD614G-SARS-CoV-2-spike:pPyEGFP using Lipofectamine 2000 (Life Technologies), and media was replaced on day 1 ...