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Citations for Thermo Fisher :
1051 - 1100 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... cells were resuspended at a concentration of 1-2×106/mL in 50-100 µL of cold HBSS containing 5% heat-inactivated FBS (Gibco), 100U/mL penicillin ...
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bioRxiv - Neuroscience 2023Quote: ... or DIV12 (overexpression) primary hippocampal neurons were transfected with 1-2 μg plasmid DNA and 5 μL Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and the nuclei pellet was resuspended in 2 mL of ice-cold resuspension buffer (1 M HEPES [#J16924- AE, ThermoFisher Scientific]; 5 M NaCl [#01094764, ThermoFisher Scientific] ...
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Bioengineering 2024Quote: ... and splenocyte to resuspend post centrifugation for 5 minutes and subsequently diluted with 5mL ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Microbiology 2023Quote: ... Staining of DNA was performed on fixed cells by incubating them for 5 min in a 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI, 62248, Thermo Fisher) solution ...
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bioRxiv - Biophysics 2023Quote: ... 300 mL NaCl, 5 mM imidazole, 10% glycerol, 0.2 mM 2-mercaptoethanol, 1 mM PMSF, and Pierce Protease Inhibitor Tablets [Thermo Fisher]). Cells were sonicated for 5 min (30% amplitude ...
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bioRxiv - Biochemistry 2023Quote: ... the buffer was exchanged to HBS buffer (1×HBS pH 7.2, 5% (v/v) glycerol) by gel filtration (Zeba Spin Desalting column, 2 mL, Thermo Fisher). For immunoblotting ...
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bioRxiv - Genomics 2023Quote: The PPMI iPSC lines were thawed and grown on matrigel (Corning)-coated plates with Essential 8 Flex (E8, Batches 1, 2 and 3) or Essential 6 (E6, Batches 4 and 5) media (both Gibco) for about one month (5 passages) ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained for 10 min in 0.5 µL/mL calcein-AM and 2 µL/mL ethidium homodimer-1 from the Live/Dead assay kit (L3224, Invitrogen). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 1–2 weeks of selection with fresh medium containing 200–500 μg/ml Hygromycin (Invitrogen, ant-hg-5). The surviving cells were released from the well with 0.05% Trypsin–EDTA (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2024Quote: C2C12 cells were magnetically labelled by an incubation with a solution of iron oxide nanoparticles at [Fe] = 1 - 2 mM and 5 mM citrate in RPMI 1640 medium (Gibco) for 30 minutes leading to an uptake of about 3 pg of iron per cell (measured by magnetophoresis) ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 7.0) and 2 µg anti-5-methylcytosine (5-mC) antibody (Thermo Fisher, 33D3), and incubated at 4°C overnight with rotation ...
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bioRxiv - Evolutionary Biology 2022Quote: ... discoideum clones on SM/5 plates (2 g glucose (Fisher Scientific), 2 g Bacto Peptone (Oxoid) ...
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bioRxiv - Neuroscience 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) (Click-iT Plus EdU Kit, Invitrogen) and 5-bromo-2’-deoxyuridine (BrdU ...
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bioRxiv - Cell Biology 2020Quote: ... Twenty min before fixation EdU (5-ethynyl-2’-deoxyuridine, Molecular probes) was added in all the experiments ...
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bioRxiv - Cancer Biology 2020Quote: ... 2×10^5 cells were lysed in RIPA buffer (Thermo Fisher) with protease and phosphatase inhibitors (Thermo Fisher) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with EdU (5-ethynyl-2’-deoxyuridine, Life technologies) at final concentration of 10 µM for 4 hours and then harvest to perform Click-iT reaction using Click-iT EdU flow cytometry Alexa Fluor 488 assay kit (Life technologies ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 5 mM MgCl2 and 2% penicillin–streptavidin (Gibco, #15140122). Minced tissue was digested with enzyme solution at 37°C for 60-90 min with gentle shaking ...
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bioRxiv - Cell Biology 2022Quote: ... 50 µM 5-ethynyl-2′-deoxyuridine (EdU) (Invitrogen, Carlsbad, CA, USA); 10 µM 5-bromo-2’-deoxyuridine (BrdU ...
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bioRxiv - Neuroscience 2020Quote: ... or 4′,6-diamidino-2-phenylindole (DAPI, 5 μg/ml, Invitrogen) were included in the secondary antibody solution to stain nuclei.
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bioRxiv - Genomics 2022Quote: ... supplemented with 5 mM MgCl2 and 2% penicillin–streptavidin (Gibco, #15140122). Tissue was incubated with enzyme solution for 30 min at 37°C with gentle shaking ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.4) supplemented with 5 μM Fura-2 AM (Thermo Fisher), 50 μM pluronic acid F-127 (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... 2% B-27 supplement and 5% fetal bovine serum (Invitrogen, Canada) plus 1/3 of minimum essential medium enriched with 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2023Quote: ... 40 µM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen, Waltham, MA, USA) was added to the cells ...
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bioRxiv - Plant Biology 2024Quote: ... respectively were stained with EdU (5-ethynyl-2′-deoxyuridine; Thermo Fisher) and modified pseudo-Schiff propidium iodide (PI ...
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bioRxiv - Cell Biology 2024Quote: ... siRNAs targeting DHHC 2 and 5 were obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Plant Biology 2024Quote: For combined 5-ethynyl-2-deoxyuridine (Invitrogen A10044, Thermo Fisher Scientific) and modified pseudo-Schiff-propidium iodide (PI ...
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bioRxiv - Plant Biology 2024Quote: For combined 5-ethynyl-2-deoxyuridine (Invitrogen A10044, Thermo Fisher Scientific) and modified pseudo-Schiff-propidium iodide (PI ...
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bioRxiv - Neuroscience 2023Quote: ... or 4°,6-diamidino-2-phenylindole (DAPI, 5 μg/ml, Invitrogen) were added during the first wash step to visualize nuclei ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in 5-ethynyl-2’-deoxyuridine (EdU; ThermoFisher A10044) dissolved in K-SFM (20 mM final concentration ...
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bioRxiv - Physiology 2024Quote: After loading with Fura-2 AM (5 μM, Invitrogen, F-1201), isolated sweat glands on coverslips were mounted in an open chamber and rinsed with standard bath solution containing (in mM) ...
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bioRxiv - Microbiology 2024Quote: ... 2 µl of 5× SYPRO orange (Life Technologies, Eugene, Oregon, USA) and 2.5 µl of the resuspended array compound or the equivalent amount of buffer in the ligand-free control ...
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bioRxiv - Systems Biology 2024Quote: ... 2) Blocking buffer: wash buffer with 5% BSA (Thermo Fisher, J6509722). Hs27-VPH were transduced with lentiviruses of pRCA360 expressing sgRNA against HES7 ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated with 5-ethynyl-2′-deoxyuridine (EdU) (Thermo Fisher Scientific, C10636), then stained with a fluorescent CD34 antibody ...
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bioRxiv - Cell Biology 2024Quote: ... transferred to 5 mL of LB broth (Fisher Scientific, BP1426-2), and incubated shaking overnight at 37℃ ...
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bioRxiv - Pathology 2020Quote: ... or Abberior STAR635P (Abberior, 2-0142-007-2, dilution 1:50) and donkey anti-rabbit conjugated to Alexa-555 (Thermo Fisher, A-31572) or Cy3 (Jackson Immuno Research ...
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bioRxiv - Immunology 2020Quote: ... Purified monoclonal antibody stock at a concentration of approximately 1mg/mL was 1:2 serially diluted in infection media (DMEM with 2% FBS and 1X penicillin-streptomycin (Thermo Fisher catalog 15140122) starting at 1:20 and ending at a dilution of 1:20,480 ...
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bioRxiv - Molecular Biology 2022Quote: ... Wells were coated with 1 μg Gfp for anti-GfpNB or 1 μg commercially available SARS-CoV-2 Spike-RBD-domain protein for SARS-CoV-2 nanobody-Cts1-fusion proteins (Invitrogen, Waltham Massachusetts, USA). Recombinant Gfp was produced in E ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels with varying PEG-αMA wt% and DTT:MMP2-degradable crosslinker ratios (Table 1) were prepared with pH 8.0 N-2-hydroxyethylpiperaine-N-2-ethane sulfonic acid (HEPES; Thermo Fisher Scientific, Waltham, MA) as a solvent to allow base-catalyzed polymerization ...
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bioRxiv - Neuroscience 2023Quote: ... then with 20µg/mL laminin for 2h at room temperature, then with a thin layer of coating medium (4mg/mL methylcellulose, 2% B27 (ThermoFisher Scientific), 1% L-Glutamine (Corning ...
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bioRxiv - Cell Biology 2021Quote: Cardiomyocytes were loaded with 2 μM Fura-2 (Thermo Fisher, F1201) in Tyrode’s solution for 15 min ...
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bioRxiv - Neuroscience 2020Quote: Cells were loaded with 2 μM fura-2 AM (ThermoFisher Scientific) in BSS for one hour at 37 °C and rinsed twice with BSS (Gibson et al ...
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bioRxiv - Microbiology 2023Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: Fura-2 pentaacetoxymethyl ester (fura-2/AM) was from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Microbiology 2022Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (Thermo Fisher), and 1X non-essential amino acids (Thermo Fisher) ...
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bioRxiv - Biochemistry 2022Quote: ... and 2 mM GlutaMax supplemented with 2% (wt/vol) B27 (GIBCO) and 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... 25 mM N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Biophysics 2024Quote: ... magnetic stirring flea (Fisher Scientific, 7 x 2 x 2 mm) in 1.5 ml conical microcentrifuge tubes (Costar ...