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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... or with three custom targeting sgRNAs (Invitrogen, Supplementary Tables 1 and 2).
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bioRxiv - Developmental Biology 2023Quote: ... with DAPI (4’, 6-Diamidino-2-Phenylindole) (Life Technologies; D1306; 1:10,000). Images were captured using a Nikon Eclipse 80i system with the NIS-Elements BR software (version 4.3 ...
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bioRxiv - Cell Biology 2023Quote: ... (1) E8 medium supplemented with 2% E8 supplement (50x) (Thermo Fisher Scientific), 1% Penicillin Streptomycin (5000U/ml ...
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bioRxiv - Microbiology 2023Quote: ... Diamidino-2-phenylindole (DAPI) (Thermo Scientific, #62247, 1:1000 dilution for IFA); Mounting medium (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... 1-2 µg of RNA was treated with DNAse I (ThermoFisher Scientific), and the cDNA was prepared using the RevertAid First Strand cDNA Synthesis kit (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... containing 1% BSA and 2 mM EDTA (Thermo Fisher Scientific, Waltham, MA) was added.
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies were Alexa Fluor 594 (1:750, 2 hrs; Invitrogen A11008) and Alexa Fluor 488 (1:750 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged at a ratio of 1:2 using TrypLE (Gibco). U2OS cells were a gift from the Chang lab and grown in McCoy’s 5A (modified ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged at a ratio of 1:2 using TrypLE (Gibco). Stem cell line H1 were purchased from WiCell (Cat # WA01) ...
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bioRxiv - Neuroscience 2023Quote: ... 2) rabbit anti-pT217-tau at 1:200 (cat # 44-744, ThermoFisher). The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human tau that contains threonine 217 ...
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bioRxiv - Neuroscience 2023Quote: ... 1 % penicillin-streptomycin and 100 µM 2-mercaptoethanol (all from Thermo Fisher), and further supplemented with 100 ng/mL Noggin (Bio-techne) ...
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bioRxiv - Biophysics 2023Quote: ... the nucleus with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, 1:200), microtubules with an anti-tubulin antibody produced in mouse (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... 2 mM L-glutamine and 1 mM sodium pyruvate (Thermo Fisher, #11360039), 100 µg/ml of Normocin (InvivoGen ...
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bioRxiv - Microbiology 2024Quote: ... 1 µL of 2 ug/µL BSA (Cat No. B14, ThermoFisher Scientific), and 1 µL of 10 ng/µL DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were selected with 2 µg mL-1 puromycin (Thermo Fisher Scientific) for 3 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary monoclonal transglutaminase-2 antibody (CUB7402, Thermo Fisher #MA5-12736, 1/15000) was diluted in 0.5 (m/v ...
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bioRxiv - Microbiology 2024Quote: ... mouse monoclonal anti-BrdU (Thermo Fisher, B35128, 1:50, 2 µg/mL). Secondary antibodies and the optimal dilutions and concentrations used are as follows ...
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bioRxiv - Systems Biology 2024Quote: ... 2 mM l-glutamine and 1% (wt/vol) nonessential amino acids (GIBCO). Medium was replaced with fresh medium every three days.
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bioRxiv - Cell Biology 2024Quote: After washing 2 times with ice-cold 1× PBS (14080-055, Gibco), cells were trypsinized or scrapped with a rubber policeman ...
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bioRxiv - Neuroscience 2024Quote: ... 1% (v/v) N-2 supplement (Gibco, Cat no: 17-502-048), and 40 ng/mL basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2024Quote: ... and AID (Clone mAID-2, ThermoFisher, 1:800, Cat # 14-5959-82)/Opal 690 ...
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bioRxiv - Immunology 2024Quote: ... rabbit anti-SARS-CoV-2 M protein 1:250 (ThermoFisher: PA1-41160), rabbit anti-SARS-CoV-2 E protein 1:250 (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... rabbit anti-SARS-CoV-2 E protein 1:250 (ThermoFisher: PA1-41158), rabbit anti-β-actin 1:250 (Abcam ...
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bioRxiv - Microbiology 2024Quote: ... and 2 µL of rhodamine-phalloidin 400X (diluted 1:500) (Invitrogen, R415) to label EPEC and F-actin ...
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bioRxiv - Immunology 2024Quote: ... and cDNA synthesis from 1-2 μg RNA using SuperScript IV (Invitrogen) using Oligo(dT ...
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bioRxiv - Immunology 2024Quote: ... THP-1 (ATCC) were maintained in R10 with 2-mercaptoethanol (Gibco, 31350010). MRC-5 (ATCC ...
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bioRxiv - Biophysics 2024Quote: ... the nucleus with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, 1:200), microtubules with an anti-tubulin antibody produced in mouse (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Thermo Fisher Scientific), 1 × Glutamax ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-beta-2-microglobulin antibody (1:100, MA5-36022, Thermo-Fisher Scientific), anti-HLA-I-ABC antibody (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... 2° Dk – α-Rb-IgG Dylight 550 (Invitrogen, SA5-10039, 1:300). Active-caspase area was quantified by manually thresholding for positive-fluorescence.
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bioRxiv - Bioengineering 2024Quote: ... 6-diamidino-2 phenylindol (DAPI, 1 ug/mL; Thermo Fisher, Waltham, MA), and AlexaFluor 488 phalloidin (1:100 ...
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bioRxiv - Molecular Biology 2020Quote: ... RD18 and JR1 cells were cultured for 2-5 days in DMEM supplemented with 2% horse serum (Gibco, Carlsbad, CA, USA) at 90-95% confluency ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed four times with PBS (2 x quick, 2 x 5 min) prior to incubating with streptavidin conjugated to AlexaFluor647 (Thermo Fisher) diluted in high-salt buffer (0.5 M NaCl ...
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bioRxiv - Cell Biology 2024Quote: RNA was extracted from ∼2-5 mg of dechorionated 0–2 hr embryos per biological replicate using TRIzol Reagent (#15596026, ThermoFisher Scientific) and treated with1 μL TURBO Dnase (2 U/μL ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Peptide mixtures were separated at a 400 nl/min flow rate using a 35 min ACN gradient in 0.5% acetic acid (5–38% ACN gradient for 23 min followed by 38-60% ACN gradient for 3 min and 60-95% ACN gradient for 2 min plus another 3 min at 95% CAN prior to 95%-5% gradient for 2 min and another 2 min at 5% ACN) and detected on an Q Exactive HF mass spectrometer (Thermo Scientific). Dynamic exclusion was enabled for 20 sec and singly charged species ...
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bioRxiv - Immunology 2021Quote: ... 1 × 105 activated NKT cells were incubated with 1 mM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Invitrogen) in RPMI complete media for 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... in a 1:1 ratio and maintained in R10 + IL-2 [RPMI 1640 (Thermo Fisher Scientific) supplemented with 10% heat-inactivated FBS (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... and switching to SLI medium (Advanced DMEM/F12 (1:1) supplemented with 2 mM GlutamaxTM (Gibco), 2% B27 without vitamin A (Life technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... and activated 1:1 with CD3/CD28 Dynabeads in 100 U/mL of IL-2 (Thermofisher). The next day ...
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bioRxiv - Bioengineering 2024Quote: ... penicillin 100U.ml-1 /streptomycin 100 μg.ml-1 and L-glutamine 2 mM (Life Technologies, Inc., USA) in 5% CO2 humidified atmosphere at 37 °C.
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bioRxiv - Cell Biology 2022Quote: ... DNA was then visualised using 1 µg ml-1 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) or 4 µM TO-PRO-3 Iodide (TOPRO ...
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bioRxiv - Bioengineering 2024Quote: ... Drug selection began 2 d post transduction with 1 μg/mL puromycin (Invitrogen, ant-pr-1). Cells were kept in antibiotics for at least two weeks with subculturing every one to two days before further characterization.
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bioRxiv - Cell Biology 2024Quote: ... IVC-1 and IVC-2 media were prepared using the following: IVC-1: DMEM/F12 (Gibco) supplemented with 20% (v/v ...
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bioRxiv - Immunology 2024Quote: ... cells were diluted 1:3 in PBS with 2 mM EDTA and 1:1000 DAPI (ThermoFisher). Cells were counted using Forward Scatter (FSC ...
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bioRxiv - Molecular Biology 2021Quote: ... in Minimum essential medium (MEM), glutamine-free based Neuronal growth medium (5% fetal bovine serum, 2% B27, 1% Glutamax (100×, Invitrogen), 2 % 1 mol/L d-glucose ...
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bioRxiv - Cell Biology 2021Quote: ... 2X SSC) for 5 min and counter stained with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies). Coverslips were mounted in imaging buffer (3.7 μg/μl glucose oxidase and 1U catalase in equilibration buffer ...
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bioRxiv - Biophysics 2021Quote: ... slides were rinsed in PBS for 3 x 5 mins and stained with 1 %g/mL 4,6-diamino-2-phenylindole (DAPI - Molecular Probes) for 3 mins in the dark at RT ...
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bioRxiv - Cell Biology 2021Quote: ... and suspension diluted 1/5 by adding FACS staining buffer (SB; 2% heat-inactivated Fetal bovine serum (Life Technologies; 26140079), 25 mM HEPES (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... and the suspension diluted 1/5 by adding FACS buffer (FB; 2% heat-inactivated Fetal bovine serum (Life Technologies; 26140079), 25 mM HEPES (Life Technologies ...