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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and NPM1 (FC-61991, Life Technologies). Secondary antibody (A11029 or A11036 ...
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bioRxiv - Developmental Biology 2020Quote: ... containing 10% FCS (Thermo Fisher Scientific) and 1% Glutamine-Penicillin-Streptomycin solution (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... supplemented with 10% FCS (#10500064, Gibco) and penicillin-streptomycin (#15070063 ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS (Fetal Calf Serum, Invitrogen) 0.5mg/mL DNase (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 10% FCS (Fisher Scientific), 100 units/ml of Penicillin/Streptomycin (Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% FCS (Thermo Scientific), 1 mM L-glutamine (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% FCS (Cellgro Gibco), 1% PEN/STREP (Cellgro Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% FCS (Gibco, 10270). For creation of the stable cell line expressing the Epac-SH189 biosensor20 transfection of HeLa cells was performed with the Tol2 transposon system23 ...
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bioRxiv - Immunology 2020Quote: ... and supplemented with 10% FCS (Gibco), 100 U/ml penicillin and 100 μg/ml streptomycin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 15% dialyzed FCS (Gibco), 1% penicillin-streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: ... 15% fetal calf serum (FCS, Invitrogen), 450 μM monothioglycerol ...
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bioRxiv - Immunology 2020Quote: ... containing 10% Heat Inactivated FCS (Invitrogen) and 1% Penicillin-Streptomycin (Merck) ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% decomplemented FCS (Gibco). Cell lines were not authenticated after purchase other than confirming the absence of mycoplasma contamination ...
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bioRxiv - Immunology 2020Quote: ... containing 0.5% Heat Inactivated FCS (Invitrogen) and 1% Penicillin-Streptomycin (Merck) ...
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bioRxiv - Bioengineering 2021Quote: ... biotin anti-FC (Thermo Fisher, A18821), by mixing the nanovial concentrate and the antibody solution at a 1:10 ratio and incubating for 10 min ...
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bioRxiv - Immunology 2021Quote: ... with 5% FCS (Thermo Fisher Scientific). The dilutions were subsequently mixed with equal volumes of 4000 TCID50/ml SARS-CoV-2 (200 TCID50/ well ...
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bioRxiv - Immunology 2020Quote: ... containing 10% Heat Inactivated FCS (Invitrogen) and 1% Penicillin-Streptomycin (Merck) ...
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bioRxiv - Immunology 2020Quote: ... anti Fc primary antibody (ThermoFisher, USA), Alexa Fluor® 594 conjugate secondary antibody (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... supplemented 10% FCS (Thermo Fisher Scientific) and 1 % ampicillin at a cell density of 3×106/mL per condition ...
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bioRxiv - Immunology 2022Quote: ... and 1:200 Fc block (Invitrogen) in 1×PBS (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... medium supplemented with 10% FCS (Invitrogen), 100 µg/ml streptomycin and 100 U/ml penicillin (Sigma) ...
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bioRxiv - Genomics 2024Quote: ... both supplemented with 10% FCS (Invitrogen), 2 mM glutamine (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... 10% Fetal Calf Serum (FCS, Gibco) and 0.05 mM β-mercapto-ethanol ...
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bioRxiv - Immunology 2022Quote: ... + 1% FCS + 5 mM EDTA (Gibco)) and incubated with 5μl TruStain FcX receptor blocking solution (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FCS (Gibco, 10270). Stable cell lines were created either using Epac-SH250 biosensor or using Epac-SH201 biosensor18 ...
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bioRxiv - Immunology 2023Quote: ... A Fc-XP column (ThermoFisher, 494371201) containing anti-IgG-CH3 matrix which binds to the CH3 region (on Fc region ...
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bioRxiv - Neuroscience 2024Quote: ... 10% fetal calf serum (FCS) (Gibco), 1× penicillin/streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Immunology 2021Quote: ... RBD-His and Spike-his containing supernatants were batch purified using the HisPur™ Ni-NTA Resin (ThermoFisher Scientific). Supernatants were incubated with 6 ml of resin for 1h at RT ...
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bioRxiv - Microbiology 2024Quote: ... MIC values were measured every 3 to 4 days in 96-well plates in plate readers (Thermo Scientific Multiskan FC with SkanIt software) according to previous description (48) ...
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bioRxiv - Cell Biology 2021Quote: Human LAMP2A was synthesized into pUC57 Plasmid with the sequence: (5’-CAAGTTTGTACAAAAAAGCAGGCTCTCGAGCAatgGTGTGCTTCCGCCTCTTCCCGGTTCCGGGCTCAGGGCTCGTTCTGGTCTGCCTAGTCCTGGGAGCTGTGCGGTCTTATGCATTGGAACTTAATTTGACAGATTCAGAAAATGCCACTTGCCTTTATGCAAAATGGCAGATGAATTTCACAGTACGCTATGAAACTACAAATAAAACTTATAAAACTGTAACCATTTCAGACCATGGCACTGTGACATATAATGGAAGCATTTGTGGGGATGATCAGAATGGTCCCAAAATAGCAGTGCAGTTCGGACCTGGCTTTTCCTGGATTGCGAATTTTACCAAGGCAGCATCTACTTATTCAATTGACAGCGTCTCATTTTCCTACAACACTGGTGATAACACAACATTTCCTGATGCTGAAGATAAAGGAATTCTTACTGTTGATGAACTTTTGGCCATCAGAATTCCATTGAATGACCTTTTTAGATGCAATAGTTTATCAACTTTGGAAAAGAATGATGTTGTCCAACACTACTGGGATGTTCTTGTACAAGCTTTTGTCCAAAATGGCACAGTGAGCACAAATGAGTTCCTGTGTGATAAAGACAAAACTTCAACAGTGGCACCCACCATACACACCACTGTGCCATCTCCTACTACAACACCTACTCCAAAGGAAAAACCAGAAGCTGGAACCTATTCAGTTAATAATGGCAATGATACTTGTCTGCTGGCTACCATGGGGCTGCAGCTGAACATCACTCAGGATAAGGTTGCTTCAGTTATTAACATCAACCCCAATACAACTCACTCCACAGGCAGCTGCCGTTCTCACACTGCTCTACTTAGACTCAATAGCAGCACCATTAAGTATCTAGACTTTGTCTTTGCTGTGAAAAATGAAAACCGATTTTATCTGAAGGAAGTGAACATCAGCATGTATTTGGTTAATGGCTCCGTTTTCAGCATTGCAAATAACAATCTCAGCTACTGGGATGCCCCCCTGGGAAGTTCTTATATGTGCAACAAAGAGCAGACTGTTTCAGTGTCTGGAGCATTTCAGATAAATACCTTTGATCTAAGGGTTCAGCCTTTCAATGTGACACAAGGAAAGTATTCTACAGCTCAAGACTGCAGTGCAGATGACGACAACTTCCTTGTGCCCATAGCGGTGGGAGCTGCCTTGGCAGGAGTACTTATTCTAGTGTTGCTGGCTTATTTTATTGGTCTCAAGCACCATCATGCTGGATATGAGCAATTTTAGGGTACCACCCAGCTTTCTTGTACAAAGTGGACGCGT-3’) Human LAMP2A was subcloned into pLenti6 (Thermo Scientific), a Gateway adapted vector using BP/LR Clonase II (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... and Flp-In™ T-REx™-293 cells (Thermo Fisher Scientific) were cultured in GlutaMAX containing Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2020Quote: ... The virus was rescued and propagated in T-REx-293 cells (Invitrogen). Purification was by CsCl gradient ultracentrifugation ...
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bioRxiv - Immunology 2020Quote: ... Cells were grown in serum-free FreeStyle 293 Expression Media (Thermo Fisher), and the cell supernatants collected on day 6 for antibody purification ...
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bioRxiv - Cancer Biology 2020Quote: ... were suspension cultured in FreeStyle™ 293 Expression Medium (Thermo Fisher Scientific). All the cells were maintained in CO2 incubator at 37°C.
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bioRxiv - Immunology 2021Quote: ... Cells were grown in serum-free FreeStyle 293 Expression Medium (Thermo Fisher), and the cell supernatants collected on day 6 for antibody purification ...
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bioRxiv - Bioengineering 2020Quote: ... All fusion proteins were obtained via the FreeStyle 293 Expression System (Invitrogen) and subsequently purified using protein A-sepharose from the harvested cell culture supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... and transfected into the Flp-In T-Rex-293 cell line (Invitrogen) according to manufacturer guidelines ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were grown in Expi 293 Expression Medium (Thermo Fisher Scientific, USA) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Transfection was performed using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell culture supernatant was collected four days after transfection and supplemented with 10x PBS to a final concentration of 2.5x PBS (342.5 mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... NIH) grown in FreeStyle 293 Expression Medium (Gibco, Thermo Scientific, MA, USA) at 37°C ...
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bioRxiv - Immunology 2022Quote: ... NIH) grown in FreeStyle 293 Expression Medium (Gibco, Thermo Scientific, MA, USA) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Transfections were performed using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell culture supernatant was collected three days after transfection and supplemented with 10x PBS to a final concentration of 2.5x PBS (342.5 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... Transfections were performed using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) using RBD expression plasmids produced at ATUM ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 suspension cells (growing in FreeStyle™ 293 Expression Medium, Gibco #12338) were transiently transfected with the vector coding for the scFv-Fc of each antibody ...
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bioRxiv - Immunology 2020Quote: ... FreeStyle™ 293F cells for protein production were obtained from Life Technologies (#R79007) and cultured in FreeStyle™ 293 Expression Medium (Life Technologies, #12338018). Chemically competent DH5a E ...
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bioRxiv - Immunology 2022Quote: ... were cultured in Freestyle 293 Expression Medium (Thermo Fisher Scientific, Waltham, MA) at 125 rpm oscillation ...
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bioRxiv - Biochemistry 2022Quote: ... Mammalian cells (tsA-201 cell line) grown in FreeStyle 293 media (Gibco) supplemented with 1% FBS were used for protein expression ...
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bioRxiv - Immunology 2021Quote: ... The culture was transfected using ExpiFectamine™ 293 Transfection Kit (Gibco, #A14524) and cultivated for 7 days ...
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bioRxiv - Immunology 2021Quote: ... by transfection with purified DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher). Supernatants from transfected cells were harvested 4 days post-transfection ...