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Citations for Thermo Fisher :
951 - 1000 of 6730 citations for GM CSF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The empty coding vector pcDNA3.1/MYC-His was obtained from Invitrogen and pCMV-β-Galactosidase was obtained from Clontech/Takara Bio.
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bioRxiv - Molecular Biology 2022Quote: ... the following antibodies were used: 6x-His Tag monoclonal antibody (ThermoFisher, cat ...
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bioRxiv - Microbiology 2023Quote: ... 1-2 μl of primary anti-His (PA1-983B, Thermo Scientific) or anti-alpha RNAP (4RA2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL of His-Tag magnetic beads (Dynabeads™ Invitrogen #10103D) was added to each tube ...
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bioRxiv - Microbiology 2023Quote: ... and inserted into similarly digested pcDNA4-To/myc-His B (Invitrogen), resulting in pcDNA4-L3HYPDH ...
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bioRxiv - Microbiology 2023Quote: ... 10% heat-inactivated fetal bovine serum (HI-FBS; Hyclone and Gibco), and 100 U/mL penicillin/streptomycin (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were taken for immunoblotting using anti His-tag antibody (ThermoFisher). Crosslinking and control reactions intended for scramblase activity assays were stopped with 1 M Tris pH 7 to a final concentration of 10 mM ...
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bioRxiv - Biochemistry 2023Quote: ... All media was supplemented with 10% HI FBS (Gibco cat # 10438026).
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% (v/v) heat-inactivated FBS (HI-FBS; Gibco). Immortalised adult human C20 microglial cells were kindly provided by Dr David Alvarez Carbonell ...
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bioRxiv - Biochemistry 2023Quote: ... Hi Five cells grown in Sf-900 II SFM media (Gibco) at a density of 2.0 × 106 cells/mL were infected with baculovirus at 1.5% v/v ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with Novex Hi-Density TBE Sample buffer (ThermoFisher Scientific, LC6678) and analysed on 10% Novex TBE gels (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... His-HcpGA1 was purified using the ProBond Purification System (Life Technologies) and the eluted protein was dialyzed against PBS and used as immunogen for antiserum generation.
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (HI-FCS, Gibco) and 1% of Penstrep (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% fetal bovine serum (Gibco FBS HI, Cat# A5256701) and 1% penicillin-streptomycin (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... followed by Western blot analysis using anti-His antibody (Invitrogen, USA).
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bioRxiv - Microbiology 2023Quote: ... 2 µL of Novex Hi-Density TBE 5X Sample Buffer (Invitrogen) was mixed with 8 µL of the completed EMSA reaction and loaded on an 8% acrylamide gel ...
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bioRxiv - Biophysics 2023Quote: ... To cut the His-tag HRV 3C protease (Thermo-Fisher Scientific) was added to a molar ratio of 1:800 (enzyme ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were re-suspended in 5ml culture medium plus macrophage colony stimulation factor (M-CSF) (Affymetrix eBioscience) at a concentration of 50ng/ml and counted on a Nucleocounter NC-250 ...
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bioRxiv - Genetics 2021Quote: ... the cells were cultured in 10% FBS of RPMI-1640 supplemented with mouse M-CSF (Thermo Fisher). Medium was replenished every 3 days and day-6 macrophages were used for experiment.
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bioRxiv - Bioengineering 2019Quote: ... and macrophage colony-stimulating factor (M-CSF, Gibco, 1µg per 5mL medium, Cat. no. PHC9501, Gibco®) in a T-25 culture flask for 5 days.
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bioRxiv - Cell Biology 2021Quote: ... 100 μL CSF-arrested or interphase extract reactions were added to 10 μL Protein A Dynabeads (Invitrogen) coupled to 5 μg rabbit anti-xCENP-C antibody ...
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bioRxiv - Immunology 2022Quote: ... purified HIVJR-CSF gp120 protein was biotinylated using EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific) and excess biotin was removed using a Zeba spin desalting column (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescent CSF tracer (albumin-Alexa Fluor™ 555, Cat No. A34786, Thermo Fisher Scientific, Waltham, MA, USA) was added to the artificial CSF at a concentration of 5 mg/ml ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 cells were transfected with pCMV-AT1R-IRES-Puro gene using Lipofectamine 3000 reagent (Thermo Fisher Scientifics). Approximately 24 hours posttransfection ...
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bioRxiv - Immunology 2021Quote: HEK293 and RAW cells were transfected using Lipofectamine 3000 or Lipofectamine LTX Transfection Reagent (Life Technologies, # L3000015) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: ... not commercially obtained but can be provided on request) (42) and HEK293-FT (R70007, Thermo Fisher Scientific) cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Physiology 2021Quote: ... The expression vector was co-transfected with pOG44 into Flp-In T-REx HEK293 cells (Life Technologies), in accordance with the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Cells were cultured in DMEM (HEK293, Neuro-2a) or RPMI 1640 (SH-SY5Y) with 10% FBS (Gibco). Cells grew on petri dishes to a density of 1×105/cm2 ...
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bioRxiv - Biochemistry 2020Quote: Immunoprecipitation from HEK293 cell lysates was conducted using Dynabeads protein G magnetic beads (Invitrogen, Thermo Fisher Scientific) according to manufacturer’s protocol with a following modification ...
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bioRxiv - Biochemistry 2020Quote: Immunoprecipitation from HEK293 cell lysates was conducted using Dynabeads protein G magnetic beads (Invitrogen, Thermo Fisher Scientific) according to manufacturer’s protocol with a following modification ...
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bioRxiv - Bioengineering 2022Quote: ... hACE-2-expressing HEK293 cells were seeded on a Nunc Lab-Tek Chamber Slide system (Thermo Fisher) and cultured overnight ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293-PSMB2-HB cells were transfected with 5 μg of the cloned plasmid using Lipofectamine 3000 (Invitrogen). After 48 h incubation ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 Jump-In and Flp-In TREx cells that allow doxycycline-dependent transgene expression were from Invitrogen. Cell lines were checked for mycoplasma infection by PCR or ELISA ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293-based EGFP reporter and Hela cells were maintained in Dulbecco’s Modified Eagle’s medium (DMEM, ThermoFisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... growth media containing DMEM (HEK293) or DMEM/F12 (CHO) (both high glucose, pyruvate, Thermo Fisher Scientific (TFS)) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293S GnTI- cells were grown at 37°C with 8% carbon dioxide in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... Transfected HEK293 cells were solubilized in 0.1-1% (w/v) n-dodecyl-β-D-maltoside (ThermoFisher Scientific), 1 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... and 5% CO2 in DMEM (for HEK293 and HeLa) supplemented with 10% Fetal bovine serum (Invitrogen, Switzerland) and 1% of Penicillin/Streptomycin (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells grown to 30-40% confluence in 6-well dishes were transfected with Lipofectamine 2000 (Invitrogen) in 35mm plates with 0.5 μg of pLKO shRNA or pLVX expression plasmids (Takara) ...
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bioRxiv - Cell Biology 2020Quote: Hek293 cells (ATTC® CRL-1573) were grown in DMEM + GlutaMAX™-I (Gibco, Invitrogen, Paisley, UK) with 10% fetal bovine serum (Cornig #35-010 ...
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bioRxiv - Cell Biology 2020Quote: Hek293 cells (ATTC® CRL-1573) were grown in DMEM + GlutaMAX™-I (Gibco, Invitrogen, Paisley, UK) with 10% fetal bovine serum (Cornig #35-010 ...
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bioRxiv - Synthetic Biology 2019Quote: Luc14 and Gal4-EED/luc HEK293 cells were grown in Gibco DMEM high glucose 1× (Life Technologies) with 10% Tet-free Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2019Quote: ... The inter-molecular tau FRET biosensor was generated by transiently transfecting HEK293 cells using Lipofectamine 3000 (Invitrogen) with tau-GFP and tau-RFP (1:20 DNA plasmid concentration ratio) ...
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bioRxiv - Molecular Biology 2021Quote: ... were transfected into HEK293 T-Rex cells with Lipofectamine CRISPRMAX Cas9 Transfection Reagent (Thermo Fisher Scientific, CMAX00008). After 48 h of incubation ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 stable cells overexpressing Flag-INTS118 and Flag-Ago215 were cultured in DMEM media (Gibco, #11965-084) containing puromycin and supplemented with 10% FBS (Atlas Biologicals ...
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bioRxiv - Cancer Biology 2019Quote: ... Both HEK293 and HEK293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco/Invitrogen, Inc., USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Both HEK293 and HEK293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco/Invitrogen, Inc., USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: Plasmids encoding RiboTag constructs were used to transiently transfect HEK293 cells with lipofectamine 3000 (Thermo Fisher Scientific) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum (FBS; Invitrogen) and 200 U/ml of penicillin and streptomycin (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: HEK293 cells were grown and maintained in DMEM/F12 medium (Gibco/Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FCS (Eurobio ...