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Citations for Thermo Fisher :
801 - 850 of 7177 citations for GM CSF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The Flp-In T-REx HEK293 cell line was used (Thermo Fisher Scientific, R78007). Stably expressing cells were isolated by treating with 200 µg/mL hygromycin B (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Reverse transfection was used to transiently transfect HEK293 cells using Lipofectamine 3000 (Thermo Fisher), using the method provided by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... The Flp-In HEK293 cells were cultured in high glucose pyruvate medium (Gibco, #10569010), supplemented with 10% FBS (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Tetracycline-inducible cells (tetON cells) were made in HEK293 Flp-In TReX cells (Invitrogen) (gift from Nancy Maizels) ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells and RAW264.7 macrophages were cultured in DMEM/F12 (cat:11320082, Thermo Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... The experimental setup utilized an inducible T-REx HEK293 cell line (Thermo Fisher Scientific) expressing MC4-R and the inwardly rectifying K+ channel Kir7.1 mutant M125R ...
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bioRxiv - Microbiology 2024Quote: ... C-His-OipA protein was purified by using Dynabeads™ His-Tag Isolation and Pulldown magnetic bead system (Thermo Fisher Scientific, United States). His-tagged OipA protein isolation procedure was carried out according to kit instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Digestion was halted with BSC growth media (BSC-GM) comprised of DMEM+Glutamax (ThermoFisher #10566024, Waltham, MA, USA) supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... and grown at 37°C in 5% CO2 in growth medium (GM): high-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... and cultured in growth medium (GM) for mMB consisting of Ham’s F10 medium (Thermo Fisher Scientific, MA, USA), 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... or (where denoted) in growth medium (GM) containing 20% foetal bovine serum (heat-inactivated FBS; 10500-064, Gibco).
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bioRxiv - Cell Biology 2023Quote: ... were seeded at 1×104 cells/cm2 in a growth medium (GM, Dulbecco’s modified Eagle’s medium DMEM, 4.5 mg/ml glucose; Gibco™) with 10% fetal bovine serum and 1% L-glutamine (Biological Industries) ...
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bioRxiv - Bioengineering 2024Quote: ... Normal rat serum and pooled CSF collected from Sprague-Dawley rats were obtained from Invitrogen and BioIVT (USA) ...
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bioRxiv - Microbiology 2023Quote: ... Lanes reacted with anti-His antibody were incubated for 1.5 hours in mouse anti 6X His antibody (Invitrogen MA1-21315, 1:500 in TBST) and lanes reacted with S-tag antibody were incubated in rabbit anti-mouse S-tag antibody (Invitrogen PIPA 581631 ...
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bioRxiv - Biochemistry 2023Quote: ... Pre-incubated mixture of scFv and respective whole venom was mixed with high-performance His-tag binding cobalt resin beads provided with Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, USA) and incubated at 20°C for 12 hours to allow attachment of the complex on resin via His-tag ...
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bioRxiv - Biophysics 2021Quote: ... 5% Fetal Calf Serum Heat Inactivated (FCS HI, Thermo Scientific) and 200μg/mL pennicilin/streptomycin (PS) ...
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bioRxiv - Cell Biology 2021Quote: ... and cloned into pcDNA3.1D/V5-His-TOPO (Thermo Fisher Scientific). MRC-5 cells were transfected with the SOD2-V5 expression construct using Polyethylenimine “MAX” (Polysciences Inc.) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 10% heat-inactivated fetal bovine serum (HI-FBS) (Gibco) at 37°C in the presence of 5% carbon dioxide (CO2) ...
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bioRxiv - Bioengineering 2021Quote: ... The anti-His(C-term)-HRP antibody (R931-25, Invitrogen) was used ...
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bioRxiv - Neuroscience 2020Quote: ... cells resuspended in 500µl 2% HI-FBS + PBS (ThermoFisher 12484028), and passed through a 40µm nylon mesh cell strainer (FisherScientific 352235) ...
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bioRxiv - Biochemistry 2020Quote: ... 6x-His tag Antibody (MA1-125 21315) was from ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... for over-expression or pcDNA4/TO/myc-his-B (Thermofisher #V103020 ...
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bioRxiv - Neuroscience 2020Quote: The backbone plasmid pcDNA6/V5-His was purchased from Invitrogen Inc ...
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bioRxiv - Biophysics 2021Quote: ... 5% Fetal Calf Serum Heat Inactivated (FCS HI, Thermo Scientific) and 200μg/mL pennicilin/streptomycin (PS) ...
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bioRxiv - Microbiology 2020Quote: ... Whole cell lysates were added to His beads (Thermo Scientific HisPur Ni-NTA Magnetic Beads ...
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bioRxiv - Biophysics 2020Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS antibody (Thermo Fisher), and 400 nM FITC-labeled TRAP220 or NCoR peptide in a buffer consisting of 20mM potassium phosphate (pH 8) ...
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bioRxiv - Biophysics 2020Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS antibody (Thermo Fisher), and 5 nM Fluormone Pan-PPAR Green fluorescent tracer ligand (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... we first placed ITPRIPL1 protein with 6x-His antibody (Invitrogen) first and slowly rotated at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... An anti-His-tag antibody coupled to HRP (Thermo Scientific) was used for enzymatic detection ...
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bioRxiv - Cell Biology 2021Quote: ... A 1:10,000 dilution of mouse α-(6X)His (Invitrogen) primary antibody in PBST/3% BSA was applied for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... dissolved in 12μl of Hi-Di formamide (Thermo fisher, 4311320), denatured at 95°C for 5 mins followed by snapchill ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% heat-inactivated (HI)-FBS (Gibco, 10438-026), 100 ng/mL IL21 (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... All PCR reactions were performed using Hi-Fi Taq (Invitrogen) or Herculase II Fusion (Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... All PCR reactions were performed using Hi-Fi Taq (Invitrogen) with appropriate primers.
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bioRxiv - Microbiology 2021Quote: ... 10% heat inactivated fetal bovine serum (HI-FBS; Life Technologies) and 1X penicillin/streptomycin (pen/strep ...
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bioRxiv - Immunology 2020Quote: ... 1 mg of magnetic bead (Dynabeads™ His-Tag, Invitrogen) were coated with 70 μg of histidine tagged RBD ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... The Ion PI Hi-Q Sequencing 200 kit (Life Technologies) was used for all sequencing reactions and Torrent Suite™ Software 5.0 (Life Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... secondary anti-6x-His tag monoclonal antibody (Invitrogen, # MA1-21315) at 1:175 dilutions ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were cloned into pcDNA3.1/Myc-His(+) (Invitrogen). Full-length sequences of DBA and 129 IFI207 were obtained from the cloned cDNAs ...
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bioRxiv - Cancer Biology 2022Quote: ... Blue Mix Hi-ROX (PCR Biosystems) and StepOnePlus (Applied Biosystems). The data expressed as the ratio between the expression level of IL-6 mRNA and that of Actin ...
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bioRxiv - Cell Biology 2024Quote: ... The anti-HIS antibody (MA1 21315) was purchased from Invitrogen.
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bioRxiv - Bioengineering 2024Quote: Nitrocellulose transfers were stained with anti-His (Invitrogen PA1-983B), anti-SARS-CoV-S (Absolute Antibodies CR3022) ...
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bioRxiv - Biophysics 2024Quote: 6x-His epitope tag antibody (His.H8) was purchased from Invitrogen. Monoclonal Flag M2 antibody (F1804-50UG) ...
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bioRxiv - Biophysics 2024Quote: ... 7 mL of His-Pur Ni-NTA resin (Thermo Scientific) was equilibrated in Buffer A containing 40 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... and cloned it in the pcDNA3.1/myc-His(-) plasmid (Invitrogen) in frame with a Myc-His tag to obtain the wild-type (WT ...
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bioRxiv - Neuroscience 2023Quote: ... 6x-His Tag Monoclonal Antibody (HIS.H8) (MA1-21315, Thermo Scientific) and HRP-conjugated goat anti-mouse secondary antibody (Abcam ab6789-1 MG ...
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bioRxiv - Molecular Biology 2023Quote: ... with 5% heat inactivated Fetal bovine serum (HI-FBS) (Gibco). 1 million cells per 100 μl per sample were stained with anti-Flag antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... protein expression from plasmid (derivates of pBAD-His/B (Invitrogen) in all cases except expression of VirF from pCL5 [71] ...
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bioRxiv - Biochemistry 2022Quote: ... 1 nM LanthaScreen Elite Tb-anti-His antibody (ThermoFisher #PV5895), and 400 nM FITC-labeled PGC1α peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...