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Citations for Thermo Fisher :
851 - 900 of 6730 citations for GM CSF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 1 nM LanthaScreen Elite Tb-anti-His antibody (ThermoFisher #PV5895), and 400 nM FITC-labeled PGC1α peptide in a buffer containing 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Biophysics 2023Quote: ... The medium was supplemented with 10% HI-FBS (Gibco, USA), 2 mM L-glutamine ...
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bioRxiv - Biochemistry 2023Quote: ... bio-His-PADI4 was incubated with magnetic streptavidin beads (Invitrogen) (4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween overnight ...
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bioRxiv - Cell Biology 2024Quote: ... The anti-HIS antibody (MA1 21315) was purchased from Invitrogen.
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bioRxiv - Bioengineering 2024Quote: Nitrocellulose transfers were stained with anti-His (Invitrogen PA1-983B), anti-SARS-CoV-S (Absolute Antibodies CR3022) ...
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bioRxiv - Biophysics 2024Quote: 6x-His epitope tag antibody (His.H8) was purchased from Invitrogen. Monoclonal Flag M2 antibody (F1804-50UG) ...
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bioRxiv - Biophysics 2024Quote: ... 7 mL of His-Pur Ni-NTA resin (Thermo Scientific) was equilibrated in Buffer A containing 40 mM imidazole ...
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bioRxiv - Cell Biology 2024Quote: ... cells were dissociated as single cells using TrypLE and plated 25.103/cm2 in growth media (GM) (knockout DMEM (Invitrogen) supplemented with 1 mM L-glutamine ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated in artificial CSF buffer containing bis(sulfosuccinimidyl)suberate (BS3) cross-linker (2 mM, ThermoFisher) for 30 min to 4 h at 4°C with constant invert mixing ...
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bioRxiv - Microbiology 2022Quote: ... Neat CSF samples (10 μL) were mixed with equal volume PrepMan™ Ultra (Thermo-Fisher Scientific) reagent in 0.2 mL PCR 8-well strips and heated to 100 °C for 10 min (T100 ...
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bioRxiv - Neuroscience 2024Quote: Peptides extracted from CSF were analyzed with an Orbitrap Q-Exactive HF mass spectrometer (Thermo Scientific) coupled with an online Easy-nLC 1200 nano-HPLC system (Thermo Scientific) ...
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bioRxiv - Immunology 2019Quote: ... Samples were prepared using the Ion PGM™ Hi-Q™ View OT2 and Ion PGM™ Hi-Q™ View Sequencing kits (ThermoFisher Scientific) and 4 barcoded libraries were combined per Ion 316™ Chip kit (ThermoFisher Scientific) ...
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bioRxiv - Pathology 2022Quote: ... human CTRP3 (Invitrogen, PA5-115061, rabbit anti-human), α-SMA-Cy3 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Purified recombinant plasmid DNA was digested with PacI and transfected into HEK293 cells with Lipofectamine (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The fibroblasts and HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (#11965–092; Gibco, NY) supplemented with 10% FBS (#12483-020 ...
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bioRxiv - Neuroscience 2019Quote: HEK293 cells (ATCC Cat# CRL-1573, RRID:CVCL_0045) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa and HEK293 cells were cultured in DMEM with sodium pyruvate and GlutaMAX (Life Technologies 10569044) or 2 mM L-glutamine supplemented with 10% heat-inactivated fetal bovine serum (Sigma F4135 ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were subjected to drug selection in the medium with 2 μg/ml puromicin (Invitrogen) for 14 days ...
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bioRxiv - Systems Biology 2020Quote: Flp-In™ T-Rex™ HEK293 cells (Life Technologies, we did not test for mycoplasm) were cultured following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... expressing mouse ATG16L1 variants was transfected into HEK293 ATG13_KO GFP-LC3B cells via Lipofectamine 2000 (Invitrogen). Cells were selected with 2.5 μg/ml puromycin (P8833 ...
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bioRxiv - Biophysics 2020Quote: ... HEK293 cells were transfected (in 35-mm-diameter wells) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) and plated onto coverslips coated with Matrigel (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: BiP-3xFLAG HEK293 cells (0.12 × 106) were plated on 12 mm round glass coverslips (Fisher Scientific) coated with 0.15 mg/mL poly-lysine in 24-well plates ...
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bioRxiv - Plant Biology 2022Quote: HEK293 cells were transfected with the respective eYFP-TPC1-fused constructs with lipofectamine2000 (ThermoFisher, Waltham, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... siRNA duplexes (siRNA B and C) were transfected into HEK293-PSMB2-HB cells using RNAiMAX (Invitrogen) dissolved in Opti-MEM (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: HEK293 (ATCC) were transfected using Lipofectamine LTX® Reagent and OptiMEM Reduced Serum Medium (Thermo Fisher) with pCMV-MAPK14 Wt (DU1765 ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293 cells were transfected with relevant plasmids or siRNA using Lipofectamine 2000 reagent (Thermo Fisher Scientific), and harvested 48 h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... B(-)S13−) bacmids were transfected into HEK293 cells using Neon transfection system (Invitrogen, Carlsbad, CA, USA). 5 x 104 cells were resuspended in 100 μl of serum-free media containing 5ug of each mutant recombinant bacmid ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were rinsed with cold PBS then scraped in PBS+1xHALT protease/phosphatase inhibitor (ThermoFisher). The cell slurry was centrifuged for 5 min at 1,000xg at 4°C to pellet cells ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 cells were seeded at 2 ×105 cells per well in six well plates (Thermo Fisher) in DMEM/10% FBS ...
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bioRxiv - Genetics 2022Quote: ... HEK293 cell cultures were transfected with 15 μg of the plasmid DNA using Lipofectamine 2000 (Invitrogen). Transfected cells were harvested 24 hours after transfection and total RNA was isolated using RNeasy mini kit (Qiagen ...
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bioRxiv - Microbiology 2019Quote: HEK293 and A549 cells were transfected with 15 pmol of siRNA using Lipofectamine RNAiMAX (Life Technologies). siRNAs (Dharmacon ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (R780-07) (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293 cells cultured in Dulbecco’s Modified Eagle medium plus GlutaMAX (DMEM, Thermo Fisher Scientific, MA, USA) supplemented with 10 % (v/v ...
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bioRxiv - Physiology 2020Quote: ... All constructs were transfected into HEK293 cells using a TurboFect transfection reagent (ThermoFisher Scientific, Waltham, MA). The day before transfection ...
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bioRxiv - Neuroscience 2021Quote: All the cells (MEF, N2a and HEK293) were plated onto tissue culture dishes in DMEM (Invitrogen) supplemented with 10% FBS and 1% Pen/Strep (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (R780-07) (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were transfected with the gRNA vectors and correction template using Lipofectamine 2000 (Life Technologies) following the manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: The HEK293 cells were maintained in 15-cm-diameter plates in Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% heat-inactivated (30 min at 56°C ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 and MDA-MB-436 cells were obtained from ATCC and cultured in DMEM (Life Technologies). U2OS cells were obtained from ATCC and were cultured in McCoy’s 5A (Life Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were transfected with pX330 containing the target site using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). Knockout clones were obtained by limiting dilution and KO confirmed by sequencing the target sites in the genome DNA and by flow cytometric analysis of GPI-APs ...
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bioRxiv - Immunology 2020Quote: ... HEK293 T cells were transfected with lipofectamine 3000 following the manufacturer’s procedure (Invitrogen, Carlsbad, CA, USA). Prefusion F (BV2129 ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293 cells were transfected with the indicated expression vectors using Lipofectamine 3000 (Life Technologies L3000-015). 48 h after transfection ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: ... HEK293 cells were transfected with the control and ADARB1 encoding pcDNA3 constructs using Lipofectamine 3000 (Invitrogen) and analyzed 72hrs post-transfection.
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured at 37°C and 5% CO2 in DMEM (Thermo Fisher Scientific; 11965092) supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Lentiviral supernatants were generated by transient transfection of HEK293 cells according to Lipofectamine ™ 2000 (Invitrogen) transfection protocols and harvested 48 h after transfection ...
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bioRxiv - Genomics 2022Quote: HUVECs and pB-circRNA transfected HEK293 were grown in media supplemented with exosome-depleted (Life Technologies). HEK293 were induced with 3 μg/ml doxycycline (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... BAC DNA was transfected into HEK293/ACE2/TMPRSS2 cells using lipofectamine 3000 (Thermo Fisher, cat. L3000001). Two days after transfection ...
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bioRxiv - Cancer Biology 2022Quote: Culturing of HEK293 (RRID:CVCL_0045) and HeLa cells (RRID:CVCL_0030) and their transfection using TurboFect (Thermo Fisher Scientific) was essentially done as described (Kessels et al. ...
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bioRxiv - Biophysics 2023Quote: 15 mL of HEK293 cells were plated at 0.625 × 106 cells / mL in DMEM (Gibco 11995065) supplemented with 10% FBS in a T75 flask ...