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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for Canine Adenovirus 2 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μL of capture Ab (~4 μg/ml) or SARS-CoV-2 recombinant antigen (~0.2 mg/ml) in 1x PBS (Gibco) was loaded in each channel ...
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bioRxiv - Cancer Biology 2023Quote: ... The enzymatic antigen retrieval was performed by incubating the sections for 2 min with TrypLE™ Express Enzyme (ThermoFisher) at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... brain slices underwent an additional antigen retrieval step in 1X IHC Antigen Retrieval Solution (Invitrogen, 00-4956-58) for 15 minutes at 95°C prior to blocking.
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bioRxiv - Neuroscience 2020Quote: ... which supplies the necessary adenovirus gene products required for the AAV production into the 293FT cell line (Invitrogen) utilizing the 293fectin transfection reagent (Invitrogen) ...
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bioRxiv - Microbiology 2019Quote: ... and Madin-Darby canine kidney (MDCK) cells (ATCC) were maintained in cell culture media (Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2020Quote: ... cells (ATCC) and Madin-Darby canine kidney (MDCK) cells (ATCC) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) supplemented with 10% FBS ...
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A Polybasic Domain in aPKC Mediates Par6-Dependent Control of Membrane Targeting and Kinase ActivitybioRxiv - Cell Biology 2020Quote: ... Madin-Darby canine kidney (MDCK) II cells were cultured in MEMα media containing 10% fetal bovine serum (Gibco) and 1% penicillin and streptomycin (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... MDCK (Mardin–Darby canine kidney) and Vero E6 cells (ATCC, no.1586) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) with 10 % FBS ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Madin-Darby Canine Kidney (MDCK) cells were transfected with the pcDNA4-Tbx2a construct using Lipofectamine 3000 (ThermoFisher Scientific) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Madin-Darby canine kidney (MDCK; ATCC) and HEK293T (293T) were grown in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: Madin–Darby canine kidney (MDCK) cells were grown in minimal essential medium (MEM) (Thermo Fisher Scientific, MA USA) containing 5% newborn calf serum (16010-159 ...
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bioRxiv - Immunology 2023Quote: ... Stock virus was sub-passaged through Madin-Darby canine kidney (MDCK) cells in Dulbecco’s modified Eagle’s medium (DMEM; Gibco), harvested as tissue culture supernatant and viral titres determined by cytopathic effects on MDCK cells and expressed as the mean log10 tissue culture infective dose required to kill 50% of the cells (TCID50 ...
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bioRxiv - Bioengineering 2022Quote: ... with 50 µL per injection site of vaccine formulations containing 5 µg of SARS-CoV-2 antigen (RBD-SD1, S-2P, S-PCNA1, or 6RBD-np) gently mixed 1:1 (v/v) with AddaVax (Invitrogen) adjuvant ...
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bioRxiv - Immunology 2022Quote: The uptake and processing of CSF-derived protein by antigen-presenting cells outside the CNS was evaluated by injecting 3 μl of DQ-OVA (2 mg/ml, Invitrogen) into the CSF of chronically infected C57BL/6 mice by i.c.m ...
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bioRxiv - Immunology 2020Quote: ... braziliensis (MHOM/BR/1975/M2903) soluble antigen (2 μg/well) were adsorbed in microtiter plate (Nunc-immuno Plate, Roskilde, Denmark). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... after which heat-induced antigen retrieval was performed in 1x low pH antigen retrieval solution (pH6, Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Developmental Biology 2019Quote: ... entry vectors were recombined with the adenovirus type 5 destination vector pAd5-CMV-V5-DEST using LR Clonase (Invitrogen). pAd5 vectors carrying candidate gene vectors were further sequenced to confirm gene identity ...
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bioRxiv - Physiology 2023Quote: A recombinant adenovirus expressing mouse Sult2b1 (Ad-Sult2b1) was produced using the ViraPower Adenoviral Expression System (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... and Madin-Darby canine kidney (MDCK) cells were cultured in cell culture medium: Dulbecco’s modified Eagle medium (DMEM) Glutamax (Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Bioengineering 2021Quote: Human HEK293T and Madin-Darby Canine Kidney (MDCK) cells (ATCC, CRL-2936) were maintained in Dulbecco’s Minimal Essential Medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Gibco) ...
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bioRxiv - Developmental Biology 2022Quote: ... and Madin-Darby Canine Kidney (MDCK) cells (ATCC, CRL-2936) were maintained in Dulbecco’s Minimal Essential Medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... The Madin-Darby Canine Kidney II (MDCK-II) cell line was maintained in Eagle’s Minimum Essential Medium (EMEM, Gibco), supplemented with 5% heat-inactivated FBS (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: Vero cells that stably express the canine receptor CD150 (Vero-cCD150) were grown in advanced Dulbecco’s modified Eagle medium (DMEM; Gibco) supplemented with 10% (V/V ...
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bioRxiv - Neuroscience 2023Quote: ... A mild antigen retrieval step was introduced by immersing the slides into boiled antigen retrieval solution (00-4955-58, Invitrogen) for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... The phage library was incubated with biotinylated CD40-Fc fusion protein or Her2 recombinant protein (Acro biosystems) for 2 hours at RT and the phage-antigen complex was captured by Dynabeads M280 (Life technologies). The bound phages were eluted by Glycin-HCl (pH 2.2 ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike protein (Hexapro antigen from Erica Ollmann Saphire, La Jallo for Immunology) was coupled to fluorescent streptavidin beads (Thermo Fisher) and incubated with sera samples to allow antibody binding to occur ...
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bioRxiv - Microbiology 2022Quote: IgG binding to SARS-CoV-2 or MeV antigens was measured by ELISA using clear flat-bottom immuno nonsterile 96-well plates (Cat# 442404, ThermoFisher Scientific) coated overnight at 4°C with 100 ng of recombinant SARS-CoV-2 proteins or 1 μg of MeV bulk antigen (Cat# BA102VS ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen unmasking was performed by microwaving the slides for 2 × 10 min in citrate buffer solution (pH 6) (Thermo Fisher Scientific). Sections were then blocked for 1 hour at room temperature with 10% normal donkey serum (Chemicon International Inc. ...
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 RBD antigens expressed in 293T cells were used to coat the 96-well ELISA plates (Cat No. 44240421, Thermofisher, USA). 50µL (2µg/mL ...
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bioRxiv - Immunology 2020Quote: ... antigens were directly coated to Nunc-absorb (ThermoFisher) plates overnight at 4°C or captured using streptavidin ...
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bioRxiv - Immunology 2023Quote: ... Antigen printed pads were blocked with SuperBlock (ThermoFisher) for 1 hour before probed with serum samples (diluted at 1:100 in SuperBlock) ...
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bioRxiv - Physiology 2024Quote: ... heated in antigen retrieval solution (Thermo Fisher Scientific) at 100 °C for 20 min ...
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bioRxiv - Physiology 2020Quote: ... The hairpin template oligonucleotides were synthesized by Integrated DNA Technologies and were subsequently cloned into the adenovirus vector of the pAD/Block-it system (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2020Quote: ... Promoter and insert were inserted into the E1 region of adenovirus (Ad5ΔE1/E3) backbone plasmid pAd/PL-DEST through Gateway recombination following the manufacturer’s instructions (Gateway System; Invitrogen, Karlsruhe, Germany). Adeno virus stocks were titrated using the cytopathic effect in HepG2 cells as previously described58.
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bioRxiv - Cell Biology 2023Quote: Generation of replication-defective recombinant adenovirus expressing N-SREBPs under CMV promoter was performed using ViraPower Adenoviral Expression System (#K4930-00, Invitrogen) as described earlier (54) ...
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bioRxiv - Biophysics 2023Quote: ... and untagged Pro283 NP were used to produce adenovirus according to the manufacturer’s instructions (Life Technologies ViraPower Adenoviral Expression System). NP-encoding sequence was cloned from plasmid HDM_FLAG_Aichi68_NP_IRES_mCherry.
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bioRxiv - Microbiology 2019Quote: ... 4 × 105 mouse splenocytes/well were plated with either with 5 μg/ml YFV-17D NS3 ATLTYRML peptide antigen (57) or with 50 μg/ml of total Vero E6 cellular antigen in RPMI 1640 medium (Gibco, Belgium) supplemented with 10% fetal bovine serum ...
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bioRxiv - Pathology 2019Quote: ... and canine IAV was extracted using 1 ml of culture supernatant using Trizol® LS Reagent (ThermoFisher Scientific, Waltham, MA) following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Madin Darby Canine Kidney (MDCK) cell were grown in Dulbecco modified Eagle medium (DMEM) containing 10% fetal bovine serum (FBS) (Gibco), 2 mM L- and 100 U/mL penicillin-streptomycin (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: Influenza A virus (IAV) strain A/WSN/33 [H1N1 subtype] was propagated in Madin-Darby Canine Kidney (MDCK) cells (ThermoFisher). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2023Quote: ... Viruses were propagated in Madin-Darby canine kidney (MDCK) cells (IRR FR-926) in Minimal Essential Media [MEM (GIBCO™)] with 0.002μg tosyl phenylalanyl chloromethyl ketone (TPCK ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 antigen sequences were cloned into pcDNA3.4 plasmids and transfected into 293F cells utilizing the Expi293 Expression System (Life Technologies, Cat # A14635) per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Immunology 2020Quote: ... the RBD antigen (diluted to 2 μg/ml in PBS) was first adsorbed to 96-well clear Immulon 4 HBX (Thermo Scientific, #3855) plates in PBS overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: NY-BR-1 antigen positive cell line CAMA-1 A2B2M and antigen negative SKMEL28 A2B2M were stained with CellTracker™ Deep Red Dye (Invitrogen, C34565) and seeded at 15000 and 10000 cells/well ...
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bioRxiv - Neuroscience 2024Quote: ... slices underwent an additional antigen retrieval step (10 minutes in a pressure cooker in IHC Antigen Retrieval Solution (Invitrogen: 00-4956-58), followed by cooling in ice for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for surface antigens in PBS (Gibco) or Brilliant Stain Buffer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... using 20 μg/mouse of antigen in Alum (Invitrogen)7,8 ...
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bioRxiv - Immunology 2021Quote: ... Antigens were purified on C-tag column (Thermo Fisher) and quality-controlled by SDS-PAGE and for their level of endotoxin ...