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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Canine Adenovirus 2 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 25 μg F antigen plasmid and 80 μl of ExpiFectamineTM CHO reagent were mixed in 1.9 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; ThermoFisher Scientific) and 0.01% pluronic acid (Merck ...
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bioRxiv - Bioengineering 2024Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, Cat # N13195) and incubated for 30 minutes in their respective culture conditions ...
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bioRxiv - Neuroscience 2022Quote: ... 100 μM 2-mercaptoethanol (2-ME, Gibco) and 1 × Antibiotic-Antimycotic (Gibco ...
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bioRxiv - Microbiology 2019Quote: ... in the presence of 10 μg of purified Gn or NP antigens in 96 well V-bottomed culture plates (Nunc, Roskilde, Denmark). For intracellular detection of IFN-γ ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunohistochemical analyses were performed on paraffin sections with traditional antigen retrieval and colorimetric development methodologies with the following primary antibodies: anti-Collagen II (Thermo Scientific, MS235B), anti-Collagen X (Quartett ...
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bioRxiv - Neuroscience 2020Quote: HEK293T cells expressing the SV40 large T antigen (HEK293T, ATCC, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, ThermoFisher Scientific, Scoresby, VIC, Australia), supplemented with 10% heat inactivated foetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... Selection of target-binding nanobodies was performed by phage-display selections against immobilized antigen proteins on Maxisorp plates (NUNC, Thermo Fisher Scientific). The initial screening was performed against CS1and CS2 ...
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bioRxiv - Microbiology 2020Quote: ... Selection of target-binding nanobodies was performed by phage-display selections against immobilized antigen proteins on Maxisorp plates (NUNC, Thermo Fisher Scientific). The initial screening was performed against CS1and CS2 ...
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bioRxiv - Microbiology 2020Quote: ELISA was carried out to estimate the avidity and overall stability of antigen-antibody complexes using the chaotropic agent ammonium thiocyanate (NH4SCN) (Acros Organics Fisher Scientific). The plates were coated with 50 ng of PvRBP2b161-1454 in PBS and incubated overnight ...
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bioRxiv - Immunology 2022Quote: Antigen and adjuvant vaccine components were diluted in DPBS (Cytiva, Marlborough, Massachusetts, USA) and then admixed in glass vials (Thermo Fisher Scientific) prior to administration ...
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bioRxiv - Neuroscience 2021Quote: ... all sections were autoclaved for antigen retrieval and immunohistochemical stained with anti-phosphorylated antibodies AT-8 (pSer202/pThr205, MN1020, Invitrogen, 1:250). Immunolabeling was then examined using a DM4000 microscope (Leica ...
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bioRxiv - Physiology 2019Quote: ... Inclusion bodies of the antigen peptides were harvested from the transformant Escherichia coli BL21 Star (DE3) (Thermo Fisher Scientific, Waltham, MA, USA) (37) ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-TRIP4 antibodies were raised in rabbits against affinity purified N-terminally hexahistidine-tagged full-length proteins and purified with the respective antigens coupled to SulfoLink resin (Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... Washed yeast libraries were stained in dark and at 4°C with biotinylated antigens pre-conjugated with SA-PE (Streptavidin, R-Phycoerythrin Conjugate, Thermo Fisher Scientific) or SA-APC (Streptavidin ...
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bioRxiv - Immunology 2022Quote: ... A33 and B5 antigens were coupled via carboxyl chemistry to Magplex® fluorescently bar-coded beads (Luminex Corporation) with sulfo-NHS and EDC (Thermo Fisher) per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and masked epitopes were recovered with eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Invitrogen, Waltham, MA, USA, #00-4956-58). Slides were blocked for 1 hour at room temperature with blocking solution (1xPBS ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained for viability and surface antigens and then intracellularly stained using the eBioscience Intracellular Fixation and Permeabilization Kit (Thermo Fisher Scientific) using the antibodies and reagents listed in Supplemental Table 5.
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bioRxiv - Cell Biology 2019Quote: ... 2×2 binning (3.7mm^2 field area)) on the ArrayScan VTI (Thermo Scientific) and analyzed simultaneously for percentage of GFP positive cells using the Target Activation Bioapplication (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in 2-(N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Invitrogen) (20 µM ...
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bioRxiv - Physiology 2021Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in saline solution at 5 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (100 μM; ThermoFisher Scientific) with Hoechst (1 μg/mL ...
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bioRxiv - Physiology 2019Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in PBS at 5 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Glucose uptake assay was performed by incubating cells with 50 µM 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (Invitrogen) for 90 min at 37°C prior to cell staining ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in glucose-free media containing 150 μM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG Thermofisher) and incubated for 45 minutes at 37°C.
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM Tris(2-carboxyethyl)phosphine (TCEP, ThermoFisher)) supplemented with a protease inhibitor tablet (Roche ...
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bioRxiv - Genetics 2019Quote: ... 2 μl of probe (2 μM; Applied Biosystems) and 2 μl of template DNA (where extracts were normalised to 34 ng/μl) ...
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bioRxiv - Pathology 2021Quote: ... for 1 week and pepsin (1 mg/ml in 0.1N HCl) was used for antigen retrieval for immunostaining with human COMP (Thermofisher; MA1-20221, 1:100), CHOP (Santa Cruz ...
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bioRxiv - Molecular Biology 2020Quote: ... a female human embryonic kidney cell line transformed with the SV40 large T antigen was obtained from ATCC (#CRL-1573) and cultured in complete media consisting of DMEM (#11965092, GIBCO, Life Technologies; Carlsbad, CA) supplemented with 10% FBS (Seradigm Premium Grade HI FBS ...
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bioRxiv - Microbiology 2020Quote: ELISA was carried out to estimate the avidity and overall stability of antigen-antibody complexes using the chaotropic agent ammonium thiocyanate (NH4SCN) (Acros Organics Fisher Scientific). The plates were coated with 50 ng of PvRBP2b161-1454 in PBS and incubated overnight ...
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bioRxiv - Biophysics 2022Quote: ... We revealed the antigens coating with a Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody (Alexa Fluor 647, Invitrogen, ref. A-21447) and analyzed the fluorescence intensity ...
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted from cell pellets collected in QuantiFERON-TB gold assay tubes without antigen using the Ribopure Ambion RNA isolation kit (Life Technologies, California, USA), and eluted RNA treated with DNase for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... and adjusted tissue and plasma extracts were added with the antiserum against OT (1:1,000 dilution) to each antigen-coated well of a 96-well microplate (F96 maxisorp nunc-immuno plate;Thermo Fisher Scientific, Roskilde, Denmark) and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... for antigen retrieval in a pressure cooker for 10 minutes then sections were blocked in CAS-block histochemical reagent (Thermo Fisher Scientific, 008120). Permeabilization was performed with 0.5% Triton X-100 in PBS for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Both FFPE and OCT cell array samples were incubated for 5min at 100°C in 1x Antigen Retrieval Solution (ThermoFisher, 00-4956-58) using a pressure cooker ...
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bioRxiv - Pathology 2023Quote: Slides were incubated at 90°C for 20 minutes in High pH IHC Antigen Retrieval Solution (Thermo Fisher Scientific Catalog Number 00-4956) and allowed to cool to room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... by measuring the expression of pluripotency surface antigen (SSEA4 1:20, Invitrogen, 12-8843-42, and TRA1-60 1:20, Invitrogen, 12-8863-82) by flow cytometry ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 (Thermofisher). A reverse transfection approach was used to deplete AurkA and CEP135 ...
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bioRxiv - Systems Biology 2023Quote: ... 2% (ThermoFisher). Purified amplicons were quantified by Qubit™ dsDNA Quantification Assay Kits (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected with the EGFP-Caspase 2 or EGFP-Δ Caspase 2 plasmid and the synthesized miR-2 (miR-2-mimic) using Cellfectin II transfection reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Macrophages were incubated with the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (10 μM, Invitrogen, California, USA) in PBS for 30 min ...
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bioRxiv - Immunology 2021Quote: ... containing 2’-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 50 μM; Thermo Fisher Scientific). To determine FA uptake ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...