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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Canine Adenovirus 2 Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... surface antigen-labeled cells were fixed and permeabilized with the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) and incubated with the fluorochrome-labeled antibodies listed in Table S3 ...
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bioRxiv - Microbiology 2021Quote: ... Mice received 20 μg protein antigen (vaccinated) or PBS (mock-vaccinated) mixed 1:1 with Imject Alum adjuvant (Thermofisher Scientific) in a total volume of 100 μl ...
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bioRxiv - Immunology 2020Quote: Serum antibodies were purified from 25mL of remaining serum of final bleeds using antigen conjugated to NHS-activated agarose resin (ThermoFisher). Columns were washed three times with 1X PBS buffer ...
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bioRxiv - Immunology 2020Quote: To address neutrophils activation and phagocytic capacities we used pHrodo-labeled BioParticles and coated with Staphylococcus aureus (S. aureus) or Zymosan antigens (Invitrogen). pH-sensitive pHrodo dyes are useful for visualizing acidification process during phago-lysosome formation (29) ...
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bioRxiv - Immunology 2020Quote: ... Each mouse received 50 μg of antigen in 150 μL of PBS (NP40‑FICOLL) or PBS/Alum (77161, Thermo Fisher) adjuvant (2:1 ratio ...
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bioRxiv - Immunology 2021Quote: ... Thymocytes were stained for extracellular antigens before EdU detection with click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen), following the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker and then incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker after which they were incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Primary cells were immortalized by transfection of pRSVAg1 plasmid expressing Simian Vacuolating Virus 40 large T antigen (SV40T) with lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... mice were injected sub-cutaneously with 10 μg of fluorescent antigen in 100 μL mixed 1:1 with Imject Alum Adjuvant (ThermoFisher) in the upper and lower flank to target the brachial ...
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bioRxiv - Immunology 2022Quote: ... for 2-18 hours at 4°C and stained with cocktails of fluorescently labeled antibodies against intracellular antigens diluted in Permeabilization Buffer (Invitrogen) for 30 minutes at 4°C.
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bioRxiv - Developmental Biology 2022Quote: ... Overnight incubations (4°C) of the sections were carried out with mouse monoclonal anti- proliferating cell nuclear antigen (PCNA) (1:200, Invitrogen). Control sections were incubated with a mouse IgG Isotype Control (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... by RT-PCR (see methods below) and (ii) by measuring the expression of pluripotency surface antigen (SSEA4 1:20, Invitrogen, 12-8843-42 ...
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bioRxiv - Developmental Biology 2023Quote: ... de-paraffinized and re-hydrated serial sections were subjected to antigen retrieval using 1x PT module buffer 1 (Thermo Scientific) for 36 min at 93°C using a KOS microwave Histostation (Milestone) ...
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bioRxiv - Neuroscience 2023Quote: ... were conducted to remove trace PFA followed by 5 min antigen retrieval with SDS (1% Sodium Dodecyl Sulfate; Fisher Scientific) in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... Antigen retrieval was performed by heating the slides in 10 mM sodium citrate buffer pH 6.0 (Thermo Fisher, S279-3) for 4 min in a microwave and left to cool down for 10 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... for 2-18 hours at 4°C and stained with cocktails of fluorescently-labeled antibodies against intracellular antigens diluted in Permeabilization Buffer (Invitrogen) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: Human embryonic kidney cells expressing SV40 large T-antigen (HEK293T, ATCC®) were cultured in Dulbecco’s modified eagle’s medium (Gibco®) supplemented with 100 units/ml penicillin and 100 µg/ml streptomycin (pen/strep ...
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bioRxiv - Bioengineering 2023Quote: Immunostaining of AADC was performed as follows: (1) incubate the brain sections in 1X antigen retrieval solution (catalog number: 00-4955-58; Invitrogen) overnight in a 60°C water bath ...
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bioRxiv - Neuroscience 2023Quote: ... were conducted to remove trace PFA followed by 5 min antigen retrieval with SDS (1% Sodium Dodecyl Sulfate; Fisher Scientific) in PBS ...
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bioRxiv - Immunology 2024Quote: ... antigen proteins of the target were biotinylated using the EZ-link™Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher), then coupled to the fluorescent neutravidin beads (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: The human non-small cell lung cancer cell line H1299 and the human embryonic kidney cell line containing the SV40 T-antigen HEK293T were maintained in Dulbeccós Modified Eaglés Medium (DMEM, 31600091, Thermo Fisher Scientific) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... 0.5% 2-mercaptoethanol (2-ME) (GIBCO) and 1% HEPES (GIBCO) ...
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bioRxiv - Microbiology 2020Quote: ... GroEL was detected by monoclonal mouse antibody that detects antigens present on all three GroEL paralogs (MA3-023, Invitrogen, ThermoFisher Scientific). Primary antibody incubation was allowed to proceed overnight at 4° C with rocking ...
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bioRxiv - Microbiology 2020Quote: ... GroEL was detected by monoclonal mouse antibody that detects antigens present on all three GroEL paralogs (MA3-023, Invitrogen, ThermoFisher Scientific). Primary antibody incubation was allowed to proceed overnight at 4° C with rocking ...
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bioRxiv - Biophysics 2019Quote: ... K562 feeder cells expressing the cognate CAR antigen ROR1 (K562-ROR1) were used to stimulate CAR T cells and maintained in 1640 RPMI media (Life technologies) supplemented with 10 % FCS (Merck) ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated with an antibody that recognizes the Cut antigen (mouse 1:50, DSHB) followed by a fluorescent-conjugated secondary antibody (Alexa Fluor, Molecular Probes). For quantitative purposes ...
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bioRxiv - Bioengineering 2020Quote: ... and subjected to heat-induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, Fisher Scientific International, Inc., Pittsburgh, MA) at >80°C ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity to the antigen was visualized using Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary antibodies (Molecular Probes, Eugene, OR). Nuclear staining was performed with Hoechst 33258 (Polysciences ...
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bioRxiv - Immunology 2022Quote: ... B/Colorado/06/2017) were chosen as a historical antigen and were biotinylated using an EZ-Link Micro NHS-PEG4 Biotinylation Kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... with the following modifications (1) antigen retrieval was performed using low pH IHC Ag Retrieval Solution (Thermo Fisher, 00-4955-58) and subjected to heat using a steamer for 40 minutes ...
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bioRxiv - Immunology 2022Quote: ... the cells were first stained for live/dead discrimination followed by surface antigen staining and subsequently fixed using Foxp3 fixation/permeabilization buffer (Thermo Fisher) for 1 hour at 4°C ...
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bioRxiv - Immunology 2019Quote: ... mice were inoculated s.c with overnight ES antigens emulsified with an equal volume of Alum-an aluminum salt adjuvant (88) obtained from Thermo Scientific to achieve a vaccine dose of 100 μg ES in 100 μl Alum as a positive control ...
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bioRxiv - Microbiology 2020Quote: ... LAM antigen was diluted to the desired concentration in control human serum in high-recovery glass vials (Thermo Scientific, Waltham, MA) C5000-995 and incubated overnight (18−24 hrs ...
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bioRxiv - Immunology 2021Quote: ... Cytokine concentrations in the day 6 supernatants of antigen-stimulated cultures were assessed by Luminex bead-based assay (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and fusion constructs of these antigens to the full length mouse invariant chain (CD74) (NCBI RefSeq: NC_000084.6) were obtained from GeneArt (Life technologies, Paisley, UK). The fusion Thyβ4-CD74 and Thyβ10-CD74 constructs were designed as an in-frame fusion of N-terminus of the thymosin cDNA sequences to the C-terminus of the full length murine invariant chain ...
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bioRxiv - Microbiology 2020Quote: ... The antigens were then reacted with an Alexa Fluor 488-conjugated goat anti-mouse IgG H+L antibody (Thermo Fisher Scientific). The antibody titer was defined as the reciprocal of the highest dilution level at which a specific fluorescent signal was detected.
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bioRxiv - Immunology 2021Quote: ... B/Colorado/06/2017) were chosen as a historical antigen and were biotinylated using an EZ-Link Micro NHS-PEG4 Biotinylation Kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Antibodies were purified by antigen affinity chromatography using Thermo Scientific AminoLink Plus Coupling Resin according to the manufacturer’s protocol (Thermo Fisher Scientific). The generated antiserum was shown to recognize exclusively the germ cells in testes and early embryos and was used in Western-blot analysis (dilution 1:1000).
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bioRxiv - Immunology 2020Quote: ... Preformed conjugates for antigen-specific B cell sorting were made as described (46) using streptavidin conjugated to PE or AlexaFluor 647 (Life Technologies). B cell probes were made using clade B JR-CSF gp120 ...
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bioRxiv - Microbiology 2022Quote: ... 100 μg of antigen plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Microbiology 2023Quote: ... 100 μg of antigen plasmid and 320 μl of ExpiFectamineTM CHO reagent were mixed in 7.7 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...
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bioRxiv - Genomics 2023Quote: ... From this sample a cell clone was derived from primary cells isolated from bat kidney tissue and immortalized via the transfer and stable production of the Simian virus 40 Large T antigen (SV40LT). Kidneys were cut in small pieces (ca. 8 mm3) and incubated in 0.05% Trypsin-EDTA solution (Gibco # 25300-054) for 15 h at 4 ºC ...
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bioRxiv - Neuroscience 2023Quote: Low-passage-number Human embryonic kidney cells transformed with SV40 T-antigen (HEK293T) cells were cultured in DMEM (Thermo Fisher Scientific) with 10% fetal bovine serum at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... The antibody-antigen complexes were incubated overnight at 4°C and eluted following Pierce Co-Immunoprecipitation (Co-IP) Kit (Thermo Fisher). Samples were then separated by gel electrophoresis followed by immunoblotting ...
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bioRxiv - Immunology 2023Quote: ... The monomer form of the antigens was biotinylated and the subsequent tetramerization of these proteins was done with FITC-labeled avidin (Invitrogen, A821), in brief ...
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bioRxiv - Immunology 2023Quote: ... and B2(TN99-419) antigens were expressed in FreeStyle 293-F cells by transient transfection in FreeStyle 293 expression media (Thermo Fisher). Cells were co-transfected at a 4:1 ratio of plasmids encoding human metapneumovirus F and furin ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... Slides were processed through de-waxing and antigen retrieval in citrate buffer at pH 6.0 using a heat-induced epitope retrieval PT module (Thermo Fisher Scientific) before proceeding with immunostaining ...
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bioRxiv - Microbiology 2024Quote: ... 25 μg F antigen plasmid and 80 μl of ExpiFectamineTM CHO reagent were mixed in 1.9 ml of cold OptiPRO™ medium (Thermo Fisher). After the first feed on day 1 ...